000			02702nam a22002057a 4500
005			20151109084956.0
008			151109b2015 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2341-T
100			_aFarid-Ul-Haq (2013-VA-555) _917647
110			_cProf. Dr. Tahir Yaqub _95165
245			_aComparison Of Antifungal Activity Of Human Salivary Histatin Between Diabetic And Nondiabetic Individuals
260			_c2015
300			_a44p.;
502			_aHistatins are antimicrobial proteins found in human saliva. These proteins have also been observed to have the ability to aid in wound healing in various organisms. The genes HTN1 and HTN3 have been studied to govern these proteins. Histatin proteins have a vast array of antimicrobial properties. While a fungus, Candida albicans or C. albicans is a part of the human normal gut flora, it is a threat to people who have a compromised immune system. An overgrowth of the fungi belonging to the Candida family leads to candidiasis in humans, and oral candidiasis has been reported to a large extent namely in diabetic patients. The antifungal activity of histatin proteins laid the basis of the current research work. In this study, the antifungal activity of saliva from a total of 64 healthy and diabetic human samples against Candida albicans has been evaluated. The samples of both healthy and diabetic human samples belong from different age ranges: 15-25, 25-35, 35-45 and 45-55 years in order to change in antifungal activity with respect to age of an individual. Antifungal activity was observed through both agar well and agar disk diffusion methods, with agar disk diffusion methods showing positive results. According to the outcomes of this study at least 120μL of healthy saliva sample is required to create a zone of inhibition. Saliva from diabetic individuals showed no antifungal results. This occurrence led to the next part of this study involving amplification of HTN3 gene. The nucleotide sequences of both healthy and diabetic individuals were compared together and showed that the absence of antifungal activity in diabetic individuals might have reasons other than a genetic one, according to this study. The results observed from the present study indicate that healthy human saliva possesses antifungal activity against Candida albicans. In accordance Summary 39 to these results, the naturally occurring antimicrobial activity of histatin proteins present in human saliva can have immense use in the field of medicine.
650			_aDepartment of Molecular Biology and Biotechnology _911414
700			_aDr. Ali Raza Awan _95023
700			_aDr. Muhammad Tayyab _95761
942			_cTH
999			_c6499 _d6498