000			04014nam a22002057a 4500
005			20160118092728.0
008			160118b2015 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2364-T
100			_aSafdar Fareed Rabbani (2012-va-637) _919332
110			_cProf.Dr.Asim Aslam _95090
245			_aEffects Of Fish Oil On The Histopathology Of Lymphoid Organs Of Broiler Experimentally Infected With Infectious Bursal Disease Virus
260			_c2015.
300			_a53p.;
502			_aInfectious Bursal Disease has become the major poultry disease in Pakistan. The disease causes immune suppression due to damage to primary lymphoid organs (Bursa of Fabricius) in affected birds. Supplementation of fish oil enhances the immune status and subside the inflammatory conditions. So this study was designed to study the effects of fish oil on histopathology of lymphoid organs and growth performance of broiler birds. For this purpose a total number of 80 day old Hubbard broiler chicks were randomly divided into four group i.e. A, B, C and D. Group A and B were challenged with IBD Virus with known (EID50 106/ml) dose was 0.1 ml intranasal on 21th day of age, Group B and C were treated with 3% fish oil while group D acted as a negative control. Blood was collected from birds on irregular interval of days 20, 22, 29, 36 and 42 for monitoring of antibody titer against IBD virus and ND vaccine. For the histopathology the lymphoid organ were collected on day 20, 22, 29, 36 and 42. Feed consumption of group A and D during 1st week of age was significantly higher than group B and C. The broilers of group A consumed feed without fish oil significantly higher than broiler of group D while, the broilers of group C consumed feed containing fish oil significantly higher from the broilers of group D after challenged with IBD. The body weight of birds of group B and C supplemented with fish oil mixed feed was significantly (p<0.05) higher than broilers receiving feed without fish oil before challenged, however after challenge the body weight gain of group C was significantly higher than group B while that of group D was significantly (p<0.05) higher group A. The feed conversion ratio (FCR) of birds supplemented with fish oil was significantly different from the broilers receiving feed without fish oil before challenged. Summary 48 However after challenged significant difference was observed with highest value of FCR by group C, followed by group B, group A and group D. Before challenged, group C show the highest Mean titer value (606.0) for IBDV followed by group B (466.0), C (288.0) and D (106.5), However, after challenge the highest value of Mean titer for IBDV was observed in group B with 5006.0, followed by group C, A and D with Mean titer of 4608.0, 4469.0, and 4015.0, respectively on 42nd day of age. During Histopathological examination, congestion, depletion of lymphocytes, dysplasia of thymic lobules, thinning of cortex, focal necrosis, disappearance of lymph follicles and interfollicular edema like lesions were observed in the lymphoid organs of challenged birds. However, cellular hypertrophy, increased lymphocytes population and presence of lymphoblast were the prominent changes in lymphoid organs of broilers supplemented with 3% fish oil, while no pathological lesions were notified in the lymphoid organs of control group. From this study, it was concluded that Infectious Bursal Disease causes immune suppression and damage immune organs in broiler, with an associated loss of response to Newcastle disease vaccine. It was also concluded from the current study that, fish oil had beneficial effect on the antibody titer against Infectious Bursal disease virus and on growth performance in broilers. The fish oil also showed therapeutic effect against Infectious Bursal Disease.
650			_aDepartment of Pathology _94635
700			_aDr.Muti-Ur-Rehman _919333
700			_aProf.Dr.Kamran Ashraf _94897
942			_cTH
999			_c7134 _d7133