000			01696nam a22002057a 4500
005			20160307095552.0
008			160307b2015 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2393-T
100			_aZunaira Zafar (2009-VA-542) _920903
110			_cDr. Wasim Shehzad _98344
245			_aExpression And Purification Of A Potent Surface Antigen (Sag1) Of Toxoplasma Gondii In Prokaryotic Expression System
260			_c2015.
300			_a49p.;
502			_aToxoplasma gondii, an intracellular obligate parasite infects almost all warm-blooded animals including human. Toxoplasmosis, caused by T. gondii, may show minute to severe clinical results in humans. Currently, there is no vaccine available for human use. SAG1 is a major candidate of interest for vaccine development that elicits humoral as well as cellular immune response against this devastating parasite. rSAG1 that had already been ligated in pET28/His expression vector, was transformed in E. coli (BL21) host and expression was confirmed by means of SDS-PAGE and western blotting. Nickel columns were utilized for affinity based chromatographic purification of rSAG1. This purified protein was then quantified via protein quantification kit. Immunogenic recombinant SAG1 can be used in diagnostic antigen-antibody tests e.g. in ELISA. Moreover, it might be used in vaccination against T. gondii. Vaccine against this parasite may alleviate socio-economic burden on human society that ultimately modulates the health parameters for better living.
650			_aDepartment of Molecular Biology and Biotechnology _911414
700			_aDr. M. Yasir Zahoor _920904
700			_aDr. Imran Rashid _95071
942			_cTH
999			_c7693 _d7692