000 02216nam a22002057a 4500
005 20160307102410.0
008 160307b2015 xxu||||| |||| 00| 0 eng d
041 _aeng
082 _a2389-T
100 _aMuhammad Salman (2008-VA-135)
_920915
110 _cProf. Dr. Khalid Saeed
_920916
245 _aMolecular Diagnosis Of Anaplasmosis In Buffaloes
260 _c2015.
300 _a46p.;
502 _aBovine Anaplasmosis is a tick-borne haemo-rickettsail disease, caused by Anaplasma species transmitted mechanically by flies, biologically by ticks and blood contaminant fomites. It is an economically important tick-borne disease of buffalo in tropical and sub-tropical areas of the world. In current study, we developed and optimized PCR first for detecting Anaplasma at genus level in buffaloes. One hundred (100) blood samples were collected from buffaloes around the Lahore region. The stained thin blood films were examined microscopically and 37% blood samples were found positive for intra-erythrocytic bodies which were then selected for DNA extraction. The DNA was extracted using commercially available kit for eventual use in optimization of PCR for diagnosis of bovine Anaplasmosis. The primers were designed targeting 16S rRNA gene of Anaplasma. For the detection, the PCR product was run in 2% agarose gel stained with ethidium bromide and thirty seven samples showed the amplification band at 179bp. The selected samples were sent for ABI sequencing to Singapore for the accurate detection of the Anaplasma species. The sequencing results were blasted with database of Genbank and we observed homology with Anaplasma phagocytophilum. We found 37% prevalence of Anaplasmosis in buffaloes through PCR. However more studies are required to confirm the species of Anaplasma infecting buffaloes (Bobalus bobalis) by designing species specific primers. Furthermore, additional studies are needed to establish the epidemiology of Anaplasmosis by using molecular tools in different geographical areas of the country for their better control.
650 _aDepartment of Parasitology
_94290
700 _aDr. Muhammad Imran Rashid
_95074
700 _aDr. Wasim Shehzad
_98918
942 _cTH
999 _c7698
_d7697