000			02645nam a22002057a 4500
005			20160523094250.0
008			160523b2015 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2437-T
100			_aAisha Nazir (2013-VA-851) _923929
110			_cDr. Muhammad Ovais Omer _95036
245			_aEvaluation Of Antiviral Activity And Embryonic Toxicity Of Doxycycline, Ciprofloxacin Alone And In Combination With Ibuprofen Against Avian Influenza H9
260			_c2015.
300			_a95p.;
502			_aThis project was designed to analyze the antiviral and embryotoxicity of doxycycline, ciprofloxacin alone and incombination with ibuprofen against H9 virus by using embryonated chicken eggs of 10 days old. The different concentrations of these agents were taken and two fold dilutions were made. Dilutions were mixed with avian influenza H9 virus and inoculated in embryonated eggs. Eggs viability was checked during incubation at 37°c temperature. After overnight chilling, haemagglutinition test was performed for evaluation of antiviral activity. Antiviral activity of these dilutions was calculated as embryo survival percentage and positive and negative hemagglutination activity. For embryotoxicity, dilutions were made in normal saline without virus and checked the results by mortality ratio after 48 hours of incubation. The study provided information regarding antiviral activity and embryotoxicity of doxycycline, ciprofloxacin alone and incombination with ibuprofen at different concentrations. The present study showed that antiviral activity increased when used doxycycline and ibuprofen incombination. After using incombination it’s antiviral activity was high at these concentrations. Results of antiviral analysis showed that doxycycline, ciprofloxacin and ibuprofen had mild antiviral activity alone and after using combination of doxycycline and ibuprofen the antiviral activity was increased. So these agents can be used as alternative therapy against avian influenza H9 virus. The outcomes were statistically analyzed by one-way ANOVA and Post-hoc Test was used to compare difference of means. Comparative analysis of antiviral activity of doxycycline, ciprofloxacin and ibuprofen alone and in combination showed that doxycycline and ibuprofen when used incombination had comparatively strong antiviral activity. It’s antiviral activity was stronger as compare when these agents used alone. In term of embryotoxicity these agents are not toxic.
650			_aDepartment of Pharmacology and Toxicology _915285
700			_aDr. Aqeel Javeed _923930
700			_aDr. Imran Altaf _97834
942			_cTH
999			_c8497 _d8496