000			02335nam a22002057a 4500
005			20160711093717.0
008			160711b2016 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2458-T
100			_aMuhammad Abid (2014-VA-502) _924561
110			_cProf. Dr. Tahir Yaqub _95165
245			_aCharacterization And Phylogenetic Analysis Of Neuraminidase Gene Of Avian Influenza Virus Subtype H9N2
260			_c2016.
300			_a51p.;
502			_aThe H9N2 AIV are endemic in Pakistan since 1998 and causing serious outbreaks in poultry industry leading to increased morbidity and mortality, reduced egg production and reduced weight gain thus causing great economic losses. As these viruses have segmented genome so there is a lots of antigenic shift, antigenic drift and genetic reassortment which results in the production of new AIV subtypes. Besides causing significant losses the poultry industry, the H9N2 AIV pose a significant threat to public health and this issue has been pronounced with the fact that these viruses caused infections in Chinese children in 1999. This primary focus of this study was to characterize and to determine the phylogenetic relationship of the N2 gene of H9N2 AIV prevalent in Pakistan with other H9N2 viruses. A total of 10 H9N2 AIV were isolated from 100 samples and analyzed through serological and molecular tests. N2 gene of three isolates was amplified and sequenced. The isolates showed 99% homology with the H9N2 AIV recently isolated from Pakistan and their phylogenetic analysis revealed that all belonged to the same G-1 lineage and fell in clusters of more recently and closely related H9N2 viruses. There were some amino acid substitutions in different positions of the NA gene as compared to previous H9N2 viruses of Pakistan and these substitutions were the same to other H9N2 viruses isolated in 2015 from Pakistan. Due to the mutating nature of the H9N2 AIV there is need for the continuous surveillance and characterization of the prevailing H9N2 avian influenza viruses as these virus have the potential to cause serious outbreaks in poultry and also pose a significant threat to the public health.
650			_aDepartment of Microbiology _94609
700			_aDr. M. Zubair Shabbir _924562
700			_aProf. Dr. Aneela Zameer Durrani _924563
942			_cTH
999			_c8676 _d8675