000			02449nam a22002057a 4500
005			20160719095429.0
008			160719b2016 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2486-T
100			_aShahpal Shujat (2010-VA-494) _924845
110			_cDr. Maryam Javed _917643
245			_aRole Of Maoa Polymorphism In Criminal Violence Among Convicted Offenders
260			_c2016.
300			_a145p.;
502			_aCriminal violence is the violent act of crime that can be of different category such as Homicidal, Sexual and Physical violence. Criminal violent behaviour is under the control of brain having serotonergic and dopaminergic system that is under the influence of genes .MAOA gene lies under the control of serotonergic system. There are 2 polymorphic forms of MAOA gene on the basis of its activity i-e MAOA-L (Low activity allele) and MAOA-H (High activity allele). MAOA-L in males but in females MAOA-H is linked with violent behaviour in combination with environmental factors. Blood/Saliva /Buccal swabs samples (n = 20) were collected from District Jail Sheikhupura, Pakistan Organic/Inorganic method of DNA extraction was used. Primers for PCR amplification was designed using Primer3 software. PCR products were sequenced by using Sanger method from CAMB. Violent behaviour was determined by using Buss and Perry aggression Questionare Physical aggression scoring. Sequencing results were analyzed using CHROMAS software. Sequence alignment tool like CLUSTAL was used for aligning multiple sequences of convicted samples and compared with control (DNA mixture amplification) and standard samples and detected for SNP. Then SNP were detected for the amino acid change by using ExPASy software.SNP statistical analysis was done by calculating POPGENE software and association analysis was performed by using ANOVA. SNP in exon 8 at locus 43591035 of MAOA was identified that was T instead of G while in exon 13 two SNPs were identified at locus 43603089 and 43603112 .Both SNPs for exon 13 was heterozygous and changes T into A .The synymous SNPs were at locus 43591035 and Summary 73 43603089 . But the SNP at locus 43603112 was non-synonymous .The association study showed that there was no association between SNP and violence scores among convicted offenders.
650			_aDepartment of Forensic Science _95695
700			_aDr. Wasim Shehzad _98918
700			_aDr. Saadat Ali _924846
942			_cBK
999			_c8766 _d8765