000			02127nam a22002057a 4500
005			20160817091646.0
008			160817b2016 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2524-T
100			_aFehmeeda Fatima (2014-VA-775) _925603
110			_cDr. Asif Nadeem _95126
245			_aNucleotide Sequence Variation In Heat Shock Protein 70-1 Gene Of Capra Aegagrus Blythi
260			_c2016
300			_a92p.;
502			_aHeat shock protein 70 (HSP 70) plays a vital role in survival of an organism by providing cytoprotection against various kinds of stresses. Among all the HSPs present in the cell, the ubiquitous HSP 70 proteins are the most abundant and temperature sensitive. Considering the importance of HSP70-1 gene in conferring thermotolerance, present study has been designed to characterize this gene in Sindh ibex which is a wild goat species of Pakistan. The characterization of HSP70 gene might be helpful for deriving phylogenetic relationship among different species and identifying new functions among the related species. Blood/meat samples (n=25) were collected from Kirthar national park, Sindh. Standard DNA extraction method was used for DNA extraction. PCR primers were designed by Primer3 software and amplification of gene was done by Polymerase Chain Reaction. PCR product was sequenced bi-directionally by Big DyeTM Terminator on ABI 3130XL Genetic Analyzer. Multiple sequence alignment was performed for polymorphism identification. Genetic diversity was calculated by using DnaSP v.5.0. Phylogenetic analysis using the MEGA v.6.0 software package was performed and neighbor joining and UPGM trees were constructed. The results indicated that Sindh ibex HSP70.1 gene was highly similar to of domestic goat, sheep, cattle, buffalo, camel and horse which indicates their origin from a common ancestor. The results of this data might be helpful in designing effective conservation strategies for Sindh ibex.
650			_aMolecular Biology and Biotechnology _925604
700			_aDr. Maryam Javed _911391
700			_aDr. Abu Saeed Hashmi _925605
942			_cTH
999			_c9035 _d9034