000			03898nam a22002177a 4500
005			20160920093144.0
008			160920b2016 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2532-T
100			_aMohsin Hassan (2009-VA-391) _926110
110			_cDr. Saeed Saleem Ahmad _926111
245			_aImmunotherapeutictrials Of Newcastle Disease In Domestic Pigeons
260			_c2016.
300			_a48p.;
502			_aPigeon belongs to the avian family, Columbidae with in the order Columbiformes. They are strong kept by the humans for variety of purposes such as food, hobby(racing) and treatment of various diseases. However, pigeons are susceptible to a variety of diseases, but viral diseases are more common. Newcastle disease (ND) is an acute, highly contagious viral disease in birds, which can cause high level (up to 100%) mortality in chickens, the most important natural host of the disease, but it can also affect a wide variety of avian species, causing severe disease. This disease is endemic in Pakistan and has a huge impact on the poultry industry. Besides vaccination if hyper immune yolk is properly harvested and purified it can be used in field condition for the treatment of infected birds .Therefore this study had been conducted to check the effectiveness of hyper immune yolk in treatment of experimentally infected birds.Suitability in usage of hyperimmune yolk in field condition aginst NewCastle Disease.In order to get the hyper immune yolk 10 eggs from Big Bird hatchery were collected.Antibody titer against NDV in egg yolk was determined by Haemagglutination inhibition test HI their titer was 1:512 which was significant for trial.Birds were separated into five groups A, B, C, D and E. They were challenged with NDV and treated with hyper immune yolk in different concentration.Group A was given hyper immune yolk first then after 24 hour NDV was given, Antibody titers at different days were calculated .Geometric Mean Titer (GMT) values of Heamagglutination Inhibition (HI) test against NDV at day 0, 10, and 22 were log2 `2.23, log2 3.23, and log2 3.18 respectively.Group B hyper immune yolk and NDV were given simultaneously in group the GMT values of Heamagglutination Inhibition (HI) test against NDV at day 0, 10, and 22 were log2 log2 2.35, and log2 3.19 an log2 3.22 respectively.Group C NDV was given first then after 24 hour hyper immune was Summary 41 injected intramuscularly. Geometric Mean Titer (GMT) values of Haemagglutination Inhibition (HI) test against NDV at day 0, 10, and 22were log2, log2 2.47, and log2 2.99 and log23.16 respectively.In Group D NDV given first then after signs and symptoms hyper immune yolk was injected.GMT values of Heamagglutination Inhibition (HI) test against NDV at day 0, 10, and 22 were log2 2.30 log2 2.83, and log2 3.06 respectively was calculated.Group E was kept as positive control it was infected with NDV only the GMT values of Heamagglutination Inhibition (HI) test against NDV at day 0, 10, and 22 were log22.13 log2 0.00, and log2 0.00 respectively was calculated. The hyper immune yolk containing 32 units against NewCastle Disease virus was injected in group A and B induced 80% protection.The yolk containing 64 units of antibodies was given in the group C resulted into 60% protection.Hyper immune yolk consisting 128 units of antibodies injected in the group D,in which clinical sign and symptoms of disease were shown given 60% protection against the New Castle Disease. From this experiment it is inferred that usage of 128 units of antibodies can induce protection against NDV infected birds and 32 and 64 units of HIY can be used as prophylactic measure.
650			_aDepartment of Clinical Medicine _926112
650			_aCMS _926113
700			_aDr. Muhammad Avais _94930
700			_aProf. Dr. Khushi Muhammad _926114
942			_cTH
999			_c9271 _d9270