000			02743nam a22002057a 4500
005			20161024123539.0
008			161024b2016 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2588-T
100			_aHuma Minhas (2014-VA-499) _926607
110			_cDr. Muhammad Ovais Omer _95036
245			_aEvaluation Of Antiviral Activity And Embryonic Toxicity Of Ivermectin And Ibuprofen Alone And In Combination Against Avian Influenza H9
260			_c2016.
300			_a66p.;
502			_aThis project was designed to analyze the antiviral andembryotoxicity of ivermectin and ibuprofenalone and in combination against H9 virus by using embryonated chicken eggsof 10 days old. Three different concentrations of these agents were selected for current project and two fold dilutions were made. Mixing of drug dilutionnswith avian influenza H9 virus was done and administered in embryonated eggs. They were kept in incubator for 72 hours. Eggs viability was checked during incubation at 37°c temperature. After overnight chilling,haemagglutinition test was done to evaluate antiviral activity. Antiviral activity of these dilutions was calculated as embryo survival percentage and positive and negative hemagglutination activity. To checkembryotoxicity,drug dilutions were made without virus and mortality ratio was checked after 48 hours of incubation. The study provided information regarding antiviral activity and embryotoxicity of ivermectin and ibuprofen alone and incombination at different concentrations. The present study showed that antiviral activity of ivermectin was very strong at all concentrations however at higher concentration it was toxic for embryo. Results of antiviral analysis showed that ivermectinand ibuprofen had antiviral activity alone and in combination afterusing combination of ivermectin and ibuprofen the antiviral activity was further increased and embrytoxicity was also diminished by combination therapyso these agents can be used as alternative therapy against avian influenza H9 virus. The outcomes were statistically analyzed by one-way ANOVA and Post-hoc Test was used to compare difference of means. Comparative analysis of antiviral activity of ivermectin and ibuprofen alone and in combination showed that ivermectin had very strong antiviral activity but it was embryotoxic at higher concentrations when ibuprofen was used in combination then had further strong antiviral activity. It’s antiviral activity was stronger as compare when these agents used alone. In term of embryotoxicity these these agents were not toxic in combination.
650			_aPharmacology and Toxicology _915285
700			_aDr. Qamar Niaz _917208
700			_aDr. Imran Altaf _97834
942			_cTH
999			_c9474 _d9473