000 02472nam a22002057a 4500
005 20161108111158.0
008 161108b2016 xxu||||| |||| 00| 0 eng d
041 _aeng
082 _a2602-T
100 _aAhmad Raza (2008-VA-271)
110 _cDr. Iahtasham Khan
245 _aSeroprevalence Of Brucellosis In Dairy Cattle In Three Selected Districts Of Punjab, Pakistan
260 _c2016.
300 _a40p.;
502 _aBrucellosis is an infectious disease of domestic and wild animals with serious zoonotic implications in humans. The disease in animals causes tremendous economic losses. Since brucellosis is primarily an animal disease, emphasis should be given to control the disease in animal populations by adopting well organized control strategies to protect the public from the risks of acquiring this zoonosis. A total of 300 blood samples (N=300) were collected randomly from cattle species with one hundred samples (n=100) from each district of Chiniot, Sargodha and Sahiwal. Approximately 04 mL of blood was collected and transferred in a blood Vacutainer having gel containing clot activating factor, stored immediately at 4 °C in an ice box and was transported to laboratory. The serum samples were screened by RBPT which is screening test for brucellosis and it was observed that 38 animals were seropositive by RBPT. Then serum samples were further confirmed by the use of most specific and sensitive serological test known as ELISA. Out of 300 samples, 33 animals were confirmed as seropositive by Indirect ELISA. The overall prevalence in each of this district by RBPT was found to be 12%, 12% and 14 % in Chiniot, Sargodha and Sahiwal respectively. The overall prevalence in each of this district by iELISA was found to be 10%, 11% and 12 %. By RBPT, Seroprevalence in females was 13.57% based on gender, animals having abortion history had high seroprevalence for brucellosis (30%) than the non-aborted animals (10.83%) and the animals with history of retained placenta are 19.30%. Seroprevalence of iELISA based on gender showed 11.79%, cattle having history of abortion 30% and animals having history of retained placenta in cattle (24.57%). The risk factors i.e. sex, abortion, retained placenta and areas are highly significant.
650 _aEpidemiology and Public Health
700 _aProf. Dr. Muhammad Younus
700 _aDr. Muhammad Faiz Qamar
942 _cTH
999 _c9661