000			03015nam a2200205 4500
005			20161108111608.0
008			161108b2016 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2615-T
100			_aAqeela Kousar (2010-VA-303) _927073
110			_cMr. Muhammad Asad Ali _927074
245			_aIsolation, Molecular Identification And Antibiotic Resistance Pattern Of Salmonella Enterica From Fancy Birds
260			_c2016.
300			_a59p.;
502			_aSalmonellosis is a disease with serious health issues related to food borne illness and most of world’s population is suffering from it. Early diagnosis in case is very important for treatment of disease. Salmonellosis may hidden as a carrier state, acts as zoonotic components for transmission of disease. Therefore the test with more diagnostic value needs to be developed like Polymerase chain reaction after culturing and microbiological examination.Salmonella enterica infections continue to pose a significant risk for poultry industry and fancy birds. Salmonella infections have been controlled by antibiotics but in recent times antibiotic resistance in microorganisms especially in Salmonella is a global health issue. Antibiotic resistant Salmonella has further compounded the problem. Poultry isolate of Salmonella enterica (n=150) were procured from Jallo park, Safari park and household pets which are taken to Pet Centre University of Veterinary and Animal Sciences Lahore then brought to Department of microbiology UVAS Lahore and identified by biochemical testing, morphology, staining characters and genus specific PCR. Antibiotic Susceptibility was checked by disc diffusion method against amoxicillin (30μg), ampicillin (10μg), cefixime (5μg), , ceftazidime (30μg), ceftriaxone (30μg), ciprofloxacin (5μg), gentamicin (10μg), nalidixic acid and tetracycline (30μg) and resistant pattern was 100 % in ampicillin and tetracycline and 41.18% and 58.82% % in gentamicin and ciprofloxacin respectively while antibiotic show 0% resistance. Fancy birds are carriers of drug resistant Salmonellae. A total of 150 samples collected from Zoo Lahore, safari park and household pet fancy birds each of n=50. Samples will enriched by non-selective and selective media, After isolation on selective media macroscopic, biochemical analysis and microscopic examination done. DNA Summary 53 extracted from culture isolated from cloacal swabs and polymerase chain reaction performed using primers. Amplication will be observed using Agarose gel electrophoresis. Research highlighted the prevalence of Salmonella in fancy birds and its possibility of transmission to human beings. Research also provided data on antibiotic resistance in Salmonellae from fancy birds and its possible role in ever increasing problem of antibiotic resistance.
650			_aDepartment of Microbiology _94609
700			_aProf. Dr. Aftab Ahmed Anjum _921924
700			_aProf. Dr. Mansur-ud-Din Ahmad _95186
942			_cTH
999			_c9662 _d9661