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008 161214b2016 xxu||||| |||| 00| 0 eng d
041 _aeng
082 _a2620-T
100 _aJaveria (2008-VA-627)
_927497
110 _cProf. Dr. Masroor Ellahi Babar
_95179
245 _aIdentification And Expression Analysis Of Genes Involved In Obsessive Compulsive Disorder In Pakistani Population
260 _c2016.
300 _a103p.;
502 _aThe background of this study is that WHO reports that psychiatry disorders affect worldwide 0.8 to 2% population. Anxiety illnesses are a class of illness associated with unreasonable and disturbing sensation of fear and tension. There are several types of anxiety disorders, such as panic disorder, agoraphobia, specific phobia, social phobia, OCD. Obsessive-compulsive disorder is a chronic disabling condition. OCD is characterized by repetitive, intrusive thoughts, images, and impulses and by repetitive, ritualistic physical or mental acts performed to reduce the attendant anxiety. The severity of OCD depends on the amount obsessions and compulsions. The Yale-Brown Obsessive Compulsive Scale (Y-BOCS) is a reliable and consistent scoring system that can be used to categorize OCD. The major genes involve in OCD are SLC6A4, BDNF, SLC1A1 and COMT genes. The study was enrolled patients treated for OCD. Blood samples have been collected from the patients. DNA extracted from fresh blood. Primers were designed. Then DNA amplification have done by Bio-Rad thermal cycler. Then gel electrophoresis was done for PCR product quantification. PCR products precipitated and sequenced. SNPs were identified. Real-time quantitative RT-PCR was performed for each sample with TaqMan Universal PCR mastermix which showed down regulation of COMT gene in OCD patients in Pakistani population. The aim of this study was SNP identification in Pakistani Population in Obsessive Compulsive disorder and to analyze the gene expression of COMT gene involved in OCD in Pakistani Population.
650 _aMolecular Biology and Biotechnology
_927498
650 _aIBBT
_927499
650 _aPhd Theses
_917640
700 _aDr. Muhammad Wasim
_95181
700 _aProf. Dr. Muhammad Abdullah
_95804
942 _cTH
999 _c9832
_d9831