Anwar Mahmood, M
Comparative Immunogenic Evaluation Of Live Sheep Pox Vaccines Prepared In Vitro And In Vivo - 1977
Romanian sheep pox virus was pronagate1 in milk teeth susceptible sheep using Borrel techniqne.
The vaccine has a titre of 10-5 in sheep.
A dose of 0.1 ml. of dilution 1:20 was recommended for each sheep. Vaccinated sheep showed a medium size (1 ½ cm- 2 cm) skin reaction at the site of inoculation and mold fever which remained for two days.
Non of the vaccinated sheep showed any secondary lesions or ill effects.
Confluent primary monolayer of testis cells was achieved after 48 hours, CPE started 96 hours post-infection, and 80 percent CF! was noticed on 7th post-infection day.
Vaccine had titre upto 10-4.5 in vitro which correlates The titre in vivo.
0.5 Tissue culture vaccine Containing 100 TCID50 was injected sub/cut to each sheep. It conferred solid immunity.
Both the vaccines withstood challenge infection with a dose of 1 ml (10,000RD) for animal given on 14th post vaccination day.
0.5 ml dose of 1:25 dilution of Romanian virus vaccine gave larger skin reaction which lead to necrosis. Therefore, 0.1 ml dose of 1:20 dilution was used in the study.
5 ml (1.00 TCID 50) of Tissue vaccine when given to sheep did not show any untoward reactions.
Both vaccines gave cross protection against local sheep pox virus and are antigenically related.
Both vaccines showed cross immunity with each other.
The propagation of sheep pox virus in tissue culture is most economical and reliable method.
Tissue culture sheep pox virus vaccine is easy to administer and is recommended for a mass vaccination/sheep pox control programme.
Department of Microbiology
0201,T
Comparative Immunogenic Evaluation Of Live Sheep Pox Vaccines Prepared In Vitro And In Vivo - 1977
Romanian sheep pox virus was pronagate1 in milk teeth susceptible sheep using Borrel techniqne.
The vaccine has a titre of 10-5 in sheep.
A dose of 0.1 ml. of dilution 1:20 was recommended for each sheep. Vaccinated sheep showed a medium size (1 ½ cm- 2 cm) skin reaction at the site of inoculation and mold fever which remained for two days.
Non of the vaccinated sheep showed any secondary lesions or ill effects.
Confluent primary monolayer of testis cells was achieved after 48 hours, CPE started 96 hours post-infection, and 80 percent CF! was noticed on 7th post-infection day.
Vaccine had titre upto 10-4.5 in vitro which correlates The titre in vivo.
0.5 Tissue culture vaccine Containing 100 TCID50 was injected sub/cut to each sheep. It conferred solid immunity.
Both the vaccines withstood challenge infection with a dose of 1 ml (10,000RD) for animal given on 14th post vaccination day.
0.5 ml dose of 1:25 dilution of Romanian virus vaccine gave larger skin reaction which lead to necrosis. Therefore, 0.1 ml dose of 1:20 dilution was used in the study.
5 ml (1.00 TCID 50) of Tissue vaccine when given to sheep did not show any untoward reactions.
Both vaccines gave cross protection against local sheep pox virus and are antigenically related.
Both vaccines showed cross immunity with each other.
The propagation of sheep pox virus in tissue culture is most economical and reliable method.
Tissue culture sheep pox virus vaccine is easy to administer and is recommended for a mass vaccination/sheep pox control programme.
Department of Microbiology
0201,T