Jameel Ahmad
A Serological And Coprological Study Of Paramphistomiasis In Buffalo - 1997
Two hundred faecal samples, serum samples and paramphistomes used in the present study collected from main slaughter house of Lahore and from surrounding of the city.
Faecal examinations were carried out with fresh smear technique and 61.5% animals found positive for paramphistomiasis. Serum was separated from the blood samples collected from infected and suspected buffaloes for the performance of enzyme-linked immunosorbent assay. Paramphistome crude antigen was prepared from fresh stomach flukes, which contained 95.66 mg proteins/mi at 545 nm.
For performing the enzyme linked immunosorbent assay, the 96 well ELISA plates were coated with crude antigen & kept overnight & then the other procedure for the assay was followed, & the serum samples were tested with paramphistome crude antigen. In positive samples, the yellowish-brown colouration was formed.
In faecal examination, 61.5% and in the ELISA 100% of the animal found positive.
From the conclusion of the results if was found that ELISA (100%) has given more positive results then faecal examination (61.5%).
Department of Clinical Medicine & Surgery
0490,T
A Serological And Coprological Study Of Paramphistomiasis In Buffalo - 1997
Two hundred faecal samples, serum samples and paramphistomes used in the present study collected from main slaughter house of Lahore and from surrounding of the city.
Faecal examinations were carried out with fresh smear technique and 61.5% animals found positive for paramphistomiasis. Serum was separated from the blood samples collected from infected and suspected buffaloes for the performance of enzyme-linked immunosorbent assay. Paramphistome crude antigen was prepared from fresh stomach flukes, which contained 95.66 mg proteins/mi at 545 nm.
For performing the enzyme linked immunosorbent assay, the 96 well ELISA plates were coated with crude antigen & kept overnight & then the other procedure for the assay was followed, & the serum samples were tested with paramphistome crude antigen. In positive samples, the yellowish-brown colouration was formed.
In faecal examination, 61.5% and in the ELISA 100% of the animal found positive.
From the conclusion of the results if was found that ELISA (100%) has given more positive results then faecal examination (61.5%).
Department of Clinical Medicine & Surgery
0490,T