A Serological And Coprological Study Of Paramphistomiasis In Buffalo (Record no. 2243)

000 -LEADER
fixed length control field 01686nam a2200205Ia 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20150917144110.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150525s1997 xx 000 0 und d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 0490,T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Jameel Ahmad
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Khalid Pervaiz
245 #2 - TITLE STATEMENT
Title A Serological And Coprological Study Of Paramphistomiasis In Buffalo
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 1997
502 ## - DISSERTATION NOTE
Dissertation note Two hundred faecal samples, serum samples and paramphistomes used in the present study collected from main slaughter house of Lahore and from surrounding of the city.

Faecal examinations were carried out with fresh smear technique and 61.5% animals found positive for paramphistomiasis. Serum was separated from the blood samples collected from infected and suspected buffaloes for the performance of enzyme-linked immunosorbent assay. Paramphistome crude antigen was prepared from fresh stomach flukes, which contained 95.66 mg proteins/mi at 545 nm.

For performing the enzyme linked immunosorbent assay, the 96 well ELISA plates were coated with crude antigen & kept overnight & then the other procedure for the assay was followed, & the serum samples were tested with paramphistome crude antigen. In positive samples, the yellowish-brown colouration was formed.

In faecal examination, 61.5% and in the ELISA 100% of the animal found positive.

From the conclusion of the results if was found that ELISA (100%) has given more positive results then faecal examination (61.5%).
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Clinical Medicine & Surgery
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Haji Ahmed
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Muhammad Ashraf
710 ## - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Veterinary Sciences
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-05-27 0490,T 0490,T Thesis


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