Aqeela Kousar (2010-VA-303)
Isolation, Molecular Identification And Antibiotic Resistance Pattern Of Salmonella Enterica From Fancy Birds - 2016. - 59p.;
Salmonellosis is a disease with serious health issues related to food borne illness and
most of world’s population is suffering from it. Early diagnosis in case is very important for
treatment of disease. Salmonellosis may hidden as a carrier state, acts as zoonotic components
for transmission of disease. Therefore the test with more diagnostic value needs to be developed
like Polymerase chain reaction after culturing and microbiological examination.Salmonella
enterica infections continue to pose a significant risk for poultry industry and fancy birds.
Salmonella infections have been controlled by antibiotics but in recent times antibiotic resistance
in microorganisms especially in Salmonella is a global health issue. Antibiotic resistant
Salmonella has further compounded the problem. Poultry isolate of Salmonella enterica (n=150)
were procured from Jallo park, Safari park and household pets which are taken to Pet Centre
University of Veterinary and Animal Sciences Lahore then brought to Department of
microbiology UVAS Lahore and identified by biochemical testing, morphology, staining
characters and genus specific PCR. Antibiotic Susceptibility was checked by disc diffusion
method against amoxicillin (30μg), ampicillin (10μg), cefixime (5μg), , ceftazidime (30μg),
ceftriaxone (30μg), ciprofloxacin (5μg), gentamicin (10μg), nalidixic acid and tetracycline
(30μg) and resistant pattern was 100 % in ampicillin and tetracycline and 41.18% and 58.82% %
in gentamicin and ciprofloxacin respectively while antibiotic show 0% resistance. Fancy birds
are carriers of drug resistant Salmonellae.
A total of 150 samples collected from Zoo Lahore, safari park and household pet fancy
birds each of n=50. Samples will enriched by non-selective and selective media, After isolation
on selective media macroscopic, biochemical analysis and microscopic examination done. DNA
Summary
53
extracted from culture isolated from cloacal swabs and polymerase chain reaction performed
using primers. Amplication will be observed using Agarose gel electrophoresis.
Research highlighted the prevalence of Salmonella in fancy birds and its possibility of
transmission to human beings. Research also provided data on antibiotic resistance in
Salmonellae from fancy birds and its possible role in ever increasing problem of antibiotic
resistance.
Department of Microbiology
2615-T
Isolation, Molecular Identification And Antibiotic Resistance Pattern Of Salmonella Enterica From Fancy Birds - 2016. - 59p.;
Salmonellosis is a disease with serious health issues related to food borne illness and
most of world’s population is suffering from it. Early diagnosis in case is very important for
treatment of disease. Salmonellosis may hidden as a carrier state, acts as zoonotic components
for transmission of disease. Therefore the test with more diagnostic value needs to be developed
like Polymerase chain reaction after culturing and microbiological examination.Salmonella
enterica infections continue to pose a significant risk for poultry industry and fancy birds.
Salmonella infections have been controlled by antibiotics but in recent times antibiotic resistance
in microorganisms especially in Salmonella is a global health issue. Antibiotic resistant
Salmonella has further compounded the problem. Poultry isolate of Salmonella enterica (n=150)
were procured from Jallo park, Safari park and household pets which are taken to Pet Centre
University of Veterinary and Animal Sciences Lahore then brought to Department of
microbiology UVAS Lahore and identified by biochemical testing, morphology, staining
characters and genus specific PCR. Antibiotic Susceptibility was checked by disc diffusion
method against amoxicillin (30μg), ampicillin (10μg), cefixime (5μg), , ceftazidime (30μg),
ceftriaxone (30μg), ciprofloxacin (5μg), gentamicin (10μg), nalidixic acid and tetracycline
(30μg) and resistant pattern was 100 % in ampicillin and tetracycline and 41.18% and 58.82% %
in gentamicin and ciprofloxacin respectively while antibiotic show 0% resistance. Fancy birds
are carriers of drug resistant Salmonellae.
A total of 150 samples collected from Zoo Lahore, safari park and household pet fancy
birds each of n=50. Samples will enriched by non-selective and selective media, After isolation
on selective media macroscopic, biochemical analysis and microscopic examination done. DNA
Summary
53
extracted from culture isolated from cloacal swabs and polymerase chain reaction performed
using primers. Amplication will be observed using Agarose gel electrophoresis.
Research highlighted the prevalence of Salmonella in fancy birds and its possibility of
transmission to human beings. Research also provided data on antibiotic resistance in
Salmonellae from fancy birds and its possible role in ever increasing problem of antibiotic
resistance.
Department of Microbiology
2615-T