Isolation, Molecular Identification And Antibiotic Resistance Pattern Of Salmonella Enterica From Fancy Birds (Record no. 9662)
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| fixed length control field | 03015nam a2200205 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20161108111608.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 161108b2016 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2615-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Aqeela Kousar (2010-VA-303) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Mr. Muhammad Asad Ali |
| 245 ## - TITLE STATEMENT | |
| Title | Isolation, Molecular Identification And Antibiotic Resistance Pattern Of Salmonella Enterica From Fancy Birds |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2016. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 59p.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Salmonellosis is a disease with serious health issues related to food borne illness and most of world’s population is suffering from it. Early diagnosis in case is very important for treatment of disease. Salmonellosis may hidden as a carrier state, acts as zoonotic components for transmission of disease. Therefore the test with more diagnostic value needs to be developed like Polymerase chain reaction after culturing and microbiological examination.Salmonella enterica infections continue to pose a significant risk for poultry industry and fancy birds. Salmonella infections have been controlled by antibiotics but in recent times antibiotic resistance in microorganisms especially in Salmonella is a global health issue. Antibiotic resistant Salmonella has further compounded the problem. Poultry isolate of Salmonella enterica (n=150) were procured from Jallo park, Safari park and household pets which are taken to Pet Centre University of Veterinary and Animal Sciences Lahore then brought to Department of microbiology UVAS Lahore and identified by biochemical testing, morphology, staining characters and genus specific PCR. Antibiotic Susceptibility was checked by disc diffusion method against amoxicillin (30μg), ampicillin (10μg), cefixime (5μg), , ceftazidime (30μg), ceftriaxone (30μg), ciprofloxacin (5μg), gentamicin (10μg), nalidixic acid and tetracycline (30μg) and resistant pattern was 100 % in ampicillin and tetracycline and 41.18% and 58.82% % in gentamicin and ciprofloxacin respectively while antibiotic show 0% resistance. Fancy birds are carriers of drug resistant Salmonellae. A total of 150 samples collected from Zoo Lahore, safari park and household pet fancy birds each of n=50. Samples will enriched by non-selective and selective media, After isolation on selective media macroscopic, biochemical analysis and microscopic examination done. DNA Summary 53 extracted from culture isolated from cloacal swabs and polymerase chain reaction performed using primers. Amplication will be observed using Agarose gel electrophoresis. Research highlighted the prevalence of Salmonella in fancy birds and its possibility of transmission to human beings. Research also provided data on antibiotic resistance in Salmonellae from fancy birds and its possible role in ever increasing problem of antibiotic resistance. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Microbiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr. Aftab Ahmed Anjum |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr. Mansur-ud-Din Ahmad |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
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| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2016-11-08 | 2615-T | 2615-T | Thesis |