In Vitro Study Of The Effect Of Camphor On Electrophysiological Indices Of Isolated Omasum Epithelium In Calves (Record no. 13597)
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fixed length control field | 02922nam a22002057a 4500 |
005 - DATE AND TIME OF LATEST TRANSACTION | |
control field | 20170920105013.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 170920b2017 xxu||||| |||| 00| 0 eng d |
041 ## - LANGUAGE CODE | |
Language code of text/sound track or separate title | eng |
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
Classification number | 2839-T |
100 ## - MAIN ENTRY--AUTHOR NAME | |
Personal name | Uzma Yousaf (2015-VA-23) |
110 ## - MAIN ENTRY--CORPORATE NAME | |
Location of meeting | Dr. Imtiaz Rabbani |
245 ## - TITLE STATEMENT | |
Title | In Vitro Study Of The Effect Of Camphor On Electrophysiological Indices Of Isolated Omasum Epithelium In Calves |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
Year of publication | 2017. |
300 ## - PHYSICAL DESCRIPTION | |
Number of Pages | 44p.; |
502 ## - DISSERTATION NOTE | |
Dissertation note | Agriculture is a backbone of Pakistan and livestock. Omasum is the third part in ruminant’s forestomach which absorbs the fluid.The endproduct of protein metabolism is ammonia and present NH4+ in ruminants and its reabsorption linked by non-selective cation channels.Addition of cation channel modulators such as camphor to diet can enhance absorption of nitrogen in the omasum. As it is suggest that ammonia transport is enhanced by essential oils. The purpose of this study is addition of camphor on isolated omasum epithelium of calves because electrophysiological changes primarily linked to the ammonia transport through activation of non-selective cation channels.Camphor may enhance short circuit currentand ammonia uptake inomasum epithelium of calves. Ten calves of uniform weight and age were slaughtered in a local slaughter house.Omasum will be removed from the abdominal cavity and cleaned in a standard transport buffer solution. Serosal layer of omasum were removed and mounted in the” ussing chamber”. Omasum epithelium after 20 minutes replaced with ammonia containing buffer solution and changes in bioelectric parameters were observed. Camphor in concentration of 1mM wasadded to mucosal side and electrophysiological parameters like Isc and Gt were observed. Moreover, pH of serosal side waschecked after camphor treatment at time intervals 20, 60 and 90 minutes respectively to assess ammonia transport. Camphor affected the electrophysiology of isolated omasum epithelium of calves by increasing short circuit current due to increase in ammonia concentrations in the omasum. Camphoraddition in 40mM NH4Cl containing solution significantly increased current (p < 0.05). ΔIsc was also significant between the groups containing 10 and 40mM NH4Cl containing solution. Change in potential difference was non significant after addition of camphor in both groups. Conductance increased significantly by increasing concentration of ammonia.Data were analyzed by using completely randomized ANOVA followed by post-hoc test used (p<0.05). Camphor treatment affects the electrophysiological indices of isolated omasal epithelium of calves and enhances ammonium transport across omasal epithelium increasing recycling of nitrogen. This may lead to efficient protein utilization by the animals. |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical Term | Physiology |
710 ## - ADDED ENTRY--CORPORATE NAME | |
Corporate name or jurisdiction name as entry element | Dr. Sajid Khan Tahir |
710 ## - ADDED ENTRY--CORPORATE NAME | |
Corporate name or jurisdiction name as entry element | Dr. Sana Ullah Iqbal |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Koha item type | Thesis |
Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
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Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2017-09-20 | 2839-T | 2839-T | Thesis |