Toxigenic Properties Of Staphylococcus Aureus From Human And Animal Origin (Record no. 2053)

000 -LEADER
fixed length control field 02865nam a2200205Ia 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20150916131849.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150525s1984 xx 000 0 und d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 0288,T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Tayyaba Rashid
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Ata-Ur-Rehman Rizvi
245 ## - TITLE STATEMENT
Title Toxigenic Properties Of Staphylococcus Aureus From Human And Animal Origin
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 1984
502 ## - DISSERTATION NOTE
Dissertation note A total number of hundred strains of Staphylococcus aureus including 50 human and 50 horse wounds strains, were isolated on Staphylococcus medium No.110. The isolated strains were maintained on nutrient agar slants. The strains of Staphylococcus aureus were examined for the production of coagulase, fibrinolysin, alpha haemolysin,beta haemolysin and delta toxin. The isolated strains were coagulase positive but a variable coagulase activity against the plasma of human, sheep and rabbit. Human plasma gave better results than rabbit and sheep plasma. brinolytic activity of Stapylococcus aureus was tested in oxalated plasma of human, sheep and rabbit. Strains isolated from human surgical wounds were found to be more fibrinolytic as compared to horse isolates. Human and rabbit plasma were a better indicators for checking fibrinolytic activity. None of the isolated strains showed fibrinolytic activity against sheep plasma.

The isolates of Stphlococcus aureus were cultured for alpha haernolysin production in nutrient broth culture. Alpha toxin was purified and its haemolysin activity was determined qualitatively and quantitatively against human, sheep and rabbit erythrocytes. Maximum haemolytic activity was observed against rabbit erythrocytes. Out of hundred strains, sixty-eight produced alpha haemolysin including 45 from human and 23 from horse wounds.

The isolates of Staphylococcus aureus were cultured in semi-solid Heart infusion agar for the production of eta haemolysin. This toxin was purified and its haemolytic activity was determined qualitatively and quantitatively against human, sheep and rabbit erythrocytes. Naximum haemolytic activity was observed against sheep erythrocytes. Out of the hundred strains, beta haemolysin was produced by sixty-two strains including 15 from human and 47 from horses.

The isolates of Staphylococcus aureus were cultured in Brain Heart infusion agar for .the production of delta toxin. This toxin was purified and its haernolytic activity was observed qualitatively and quantitatively against human, sheep and rabbit erythrocytes. Maximum haemolytic activity of dlta toxin was observed against human erythrocytes. Out of the hundred strains, seventy-one produced delta toxin including 43 from human and 28 from horses.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Microbiology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Manzoor
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Muhammed Ajmal
710 ## - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Veterinary Sciences
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-05-26 0288,T 0288,T Thesis


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