Isolation And Characterization Of Avian Isolates Of Lactobacilli Species And Their Antisalmonella Activity (Record no. 6368)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02321nam a22002057a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20151020121242.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 151020b2015 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2313-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Anum Shaukat (2009-VA-220) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr. Muhammad Nawaz |
| 245 ## - TITLE STATEMENT | |
| Title | Isolation And Characterization Of Avian Isolates Of Lactobacilli Species And Their Antisalmonella Activity |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 78p.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Poultry industry is second largest industry of Pakistan. Poultry industry is cheaper source of protein and provides jobs for more than 1.5 million people. It is facing several problems due to microbial diseases. Salmonella is one of the leading causes of diseases in poultry. These are being treated with antibiotics but misuse and overuse of antibiotics result in antibiotic resistant strains of microorganisms. We need some alternatives for treatments. Lactobacilli are one of the alternatives to antibiotics used as probiotics. It has been found that the Lactobacilli of poultry origin have antimicrobial activity against Salmonella. Lactobacilli was isolated from the droppings, cloaca and caecum of rural poultry birds using deMan Rogosa Sharpe (MRS) medium. The isolates were screened for anti-Salmonella activity against S. enterica along with their properties to resist low pH and bile acids, antibiotic sensitivity, auto-aggregation and co-aggregation. The isolates showed anti-salmonella activity were identified using microscopic characters and biochemical profile. The isolates were confirmed by PCR using species specific primers and sequencing 16S rRNA gene. The data was analysed using one-way ANOVA at significance level P value <0.05 by using the statistical software Graph Pad Prism version 5.3. The study was conducted on a total of 60 samples including caecal swabs (n=20), cloacal swabs (n=20) and dropping (n=20) of indigenous poultry. From these samples, five isolates were Summary 57 selected based upon the tests performed. Isolates namely CLB-41, CLB-45, PDL-13, PDL-26 and PDL-33 showed best results. Further characterization was done by PCR and sequencing. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Microbiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Jawad Nazir |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr. Mansur-Ud-Din Ahmad |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-10-20 | 2313-T | 2313-T | Thesis |