Nucleotide Sequence Variation In Heat Shock Protein 70-1 Gene Of Capra Aegagrus Blythi (Record no. 9035)
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| 000 -LEADER | |
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| fixed length control field | 02127nam a22002057a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20160817091646.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160817b2016 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2524-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Fehmeeda Fatima (2014-VA-775) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr. Asif Nadeem |
| 245 ## - TITLE STATEMENT | |
| Title | Nucleotide Sequence Variation In Heat Shock Protein 70-1 Gene Of Capra Aegagrus Blythi |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 92p.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Heat shock protein 70 (HSP 70) plays a vital role in survival of an organism by providing cytoprotection against various kinds of stresses. Among all the HSPs present in the cell, the ubiquitous HSP 70 proteins are the most abundant and temperature sensitive. Considering the importance of HSP70-1 gene in conferring thermotolerance, present study has been designed to characterize this gene in Sindh ibex which is a wild goat species of Pakistan. The characterization of HSP70 gene might be helpful for deriving phylogenetic relationship among different species and identifying new functions among the related species. Blood/meat samples (n=25) were collected from Kirthar national park, Sindh. Standard DNA extraction method was used for DNA extraction. PCR primers were designed by Primer3 software and amplification of gene was done by Polymerase Chain Reaction. PCR product was sequenced bi-directionally by Big DyeTM Terminator on ABI 3130XL Genetic Analyzer. Multiple sequence alignment was performed for polymorphism identification. Genetic diversity was calculated by using DnaSP v.5.0. Phylogenetic analysis using the MEGA v.6.0 software package was performed and neighbor joining and UPGM trees were constructed. The results indicated that Sindh ibex HSP70.1 gene was highly similar to of domestic goat, sheep, cattle, buffalo, camel and horse which indicates their origin from a common ancestor. The results of this data might be helpful in designing effective conservation strategies for Sindh ibex. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Molecular Biology and Biotechnology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Maryam Javed |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Abu Saeed Hashmi |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2016-08-17 | 2524-T | 2524-T | Thesis |