Development Of Dna Based Diagnosis Of Theileriosis In Cattle And Its Specificity With Blood Smear Microscopy (Record no. 9255)

000 -LEADER
fixed length control field 02545nam a22002177a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20160919104024.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 160919b2016 xxu||||| |||| 00| 0 eng d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 2547-T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Uzma Sarwar (2014-VA-777)
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Wasim Shehzad
245 ## - TITLE STATEMENT
Title Development Of Dna Based Diagnosis Of Theileriosis In Cattle And Its Specificity With Blood Smear Microscopy
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2016.
300 ## - PHYSICAL DESCRIPTION
Number of Pages 75p.;
502 ## - DISSERTATION NOTE
Dissertation note Theileria annulata and Theileria parva are intra-erythrocytic parasites which are responsible for
causing tropical theileriosis and East Coast fever in cattle respectively. This parasite is
transmitted by ticks to vertebrate host i.e. cattle. Currently used diagnostic methods for diagnosis
of bovine theileriosis are clinical symptoms, peripheral blood smear microscopy and serological
tests (IFAT and ELISA).
Current study was conducted to compare the specificity and sensitivity of blood smear
microscopy and PCR techniques to diagnose bovine theileriosis. This study is comparative as
well as developmental in nature. Although peripheral blood smears microscopy is cost effective
and quick method of diagnosis in case of high or moderate parasitaemia in blood. But the
limitations associated with microscopy include false negative diagnosis in case of low
parasitaemia in chronic and asymptomatic infection, morphological similarity of Theileria with
other species of Plasmodium and Babesia. These limitations may lead to misdiagnose the
infection due to which disease may remain unnoticed. PCR based method, developed in this
study, and is found to be more specific and sensitive than conventional microscopy. Fifty blood
samples were collected from September, 2015 to November, 2015. These samples were screened
microscopically as well as with PCR for presence of Theileria. Nine samples were found to be
positive microscopically but 18 samples were found positive by PCR. The results obtained from
the study clearly show that PCR is more reliable, precise and sensitive assay for diagnosis of
bovine theileriosis then microscopy. It is hoped that proposed method to diagnose Theileria will
help to nullify the problems associated with microscopy. This will ultimately facilitate in the
formulation of effective treatment control and vaccine development strategies.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Molecular Biology and Biotechnology
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term IBBT
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. M. Yasir Zahoor
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Ali Ahmed Sheikh
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2016-09-19 2547-T 2547-T Thesis


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