Serological Response Of Broilers To Adjuvant Containing Avian Influenza Virus (H9 Type) Vaccine
By: Aayesha Riaz Malik
| Dr.Khushi Muhammad.
Contributor(s): Dr.Khalid Saeed | Dr.Muhammad Akram Munir | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2001Subject(s): Department of MicrobiologyDDC classification: 0723,T Dissertation note: Avian Influenza due to subtype AIV (H9 N2) caused high mortality in breeder, layer and broilers in different regions of Pakistan, during 1998 and 1999. AIV was obtained from Olympia laboratories, Lahore Road Sheikhupura. The virus was characterized by cultural characteristics in chicken embryos, virus induced death of chicken embryos within 48-72 hours post inoculation. Virus in AAF showed 1-IA titer 1:1024, while its Eli)50 was 1W 84/ml. Virus neutralization test confirmed the virus as AIV (1-19 N2).
Virus was inactivated by 0.12% formalin and then 3 adjuvant containing A1V (119 N2) vaccines were prepared with inactivated virus. The vaccines were (a) Alum precipitated Al vaccine (AP-AIV) (b) Aluminum hydroxide gel Al vaccine (AH-AIV) (c) Oil based Al vaccine (0B-AIV). Oil based vaccine was prepared with hydrophile lipophile balance (HLB) values between 5,8 and 9. The vaccine with HLB value 8 was the most stable.
Four groups of broiler birds were divided as A. B, C and D (10 birds each). 013- AIV was inoculated in birds of group A at 10th day of age and no boosting of it was required. Birds of group A showed high level of Ill antibody titer till 6 weeks of age. When AP-AIV and AHl-AIV were injected in birds of group II and C' respectively at 5' day of age and boosting with AP-A1V and AH-AIV at 14th1 day of age respectively, then birds of group B and C displayed effective antibody level till the 5 week of age
However unvaccinated birds (control group) were negative to HI antibody titer. Cumulative geometric mean titre (CGMT) of 0B-AIV. AH-AIV and AP-AIV were 124.86. 63.56 and 67.74 respectively, while the standard deviation (P>0.05) from the mean of OB-AlV, AH-AIV and AP-AIV was 78.92, 40.56 and 36.13 respectively which showed that AH-AIV and AP-AIV were non significantly different. The result indicated that each of the adjuvant containing vaccine was effective to inchice anti-HI antibody.
The cost of three vaccines was calculated and these were 285.2 Rs. for AP-AIV, 287 Rs. for AH-AIV and 365 Rs. for OB-AIV for 1000 doses of vaccines. The oil-based vaccine was cost effective and quality conscious. It is recommended for all types of commercial birds.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0723,T (Browse shelf) | Available | 0723,T |
Avian Influenza due to subtype AIV (H9 N2) caused high mortality in breeder, layer and broilers in different regions of Pakistan, during 1998 and 1999. AIV was obtained from Olympia laboratories, Lahore Road Sheikhupura. The virus was characterized by cultural characteristics in chicken embryos, virus induced death of chicken embryos within 48-72 hours post inoculation. Virus in AAF showed 1-IA titer 1:1024, while its Eli)50 was 1W 84/ml. Virus neutralization test confirmed the virus as AIV (1-19 N2).
Virus was inactivated by 0.12% formalin and then 3 adjuvant containing A1V (119 N2) vaccines were prepared with inactivated virus. The vaccines were (a) Alum precipitated Al vaccine (AP-AIV) (b) Aluminum hydroxide gel Al vaccine (AH-AIV) (c) Oil based Al vaccine (0B-AIV). Oil based vaccine was prepared with hydrophile lipophile balance (HLB) values between 5,8 and 9. The vaccine with HLB value 8 was the most stable.
Four groups of broiler birds were divided as A. B, C and D (10 birds each). 013- AIV was inoculated in birds of group A at 10th day of age and no boosting of it was required. Birds of group A showed high level of Ill antibody titer till 6 weeks of age. When AP-AIV and AHl-AIV were injected in birds of group II and C' respectively at 5' day of age and boosting with AP-A1V and AH-AIV at 14th1 day of age respectively, then birds of group B and C displayed effective antibody level till the 5 week of age
However unvaccinated birds (control group) were negative to HI antibody titer. Cumulative geometric mean titre (CGMT) of 0B-AIV. AH-AIV and AP-AIV were 124.86. 63.56 and 67.74 respectively, while the standard deviation (P>0.05) from the mean of OB-AlV, AH-AIV and AP-AIV was 78.92, 40.56 and 36.13 respectively which showed that AH-AIV and AP-AIV were non significantly different. The result indicated that each of the adjuvant containing vaccine was effective to inchice anti-HI antibody.
The cost of three vaccines was calculated and these were 285.2 Rs. for AP-AIV, 287 Rs. for AH-AIV and 365 Rs. for OB-AIV for 1000 doses of vaccines. The oil-based vaccine was cost effective and quality conscious. It is recommended for all types of commercial birds.
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