Diagnosis And Prevalence Of Trypanosoma Evansi In Camels Through Polymerase Chain Reaction (PCR) And Haematocrit Centrifugation Thechnique (HCT) in Punjab (Pakistan)
By: Jahanzaib
| Prof. Dr. Zafar Iqbal Chaudhary.
Contributor(s): Prof. Dr | Prof. Dr. H.A. Hashmi | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2005Subject(s): Department of PathologyDDC classification: 0862,T Dissertation note: The most important protozoan disease of camels is trypanosomiasis caused by Trypanosoma evansi. There was little epidemiological information on the prevalence of infection. The present study was conducted to find out the prevalence of Trypanosorna evansi in camels through haematocrit centrifugation technique (HCT) and polymerase chain reaction (PCR). A total number of 100 camels of different age and sex groups were selected from different localities including Bahawalpur, Lahore, Gujranwala and faisalabad to find out the prevalence of Trypanosomiasis in Punjab (Pakistan) and to evaluate the sensitivity of PCR assay and HCT for the diagnosis Trypanosoma evansi. Blood samples were collected and examined by haematocrit centrifugation technique (HCT) and polymerase chain reaction (PCR). The prevalence was recorded as 4% and 13% by haematocrit centrifugation technique (HCT) and polymerase chain reaction. The positive samples by the polymerase chain reaction also included the positive animals by the haermatocrit centrifugation technique.
The results showed that PCR was more sensitive method for the detection of trypanosomiasis as compared to the haematocrit centrifugation technique. Thus PCR can be used for the diagnosis of camel trypanosornosis during both acute and chronic phases of infection, and for use in the evaluation of treatment. Application of PCR to field diagnosis is therefore clearly indicated.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0862,T (Browse shelf) | Available | 0862,T |
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The most important protozoan disease of camels is trypanosomiasis caused by Trypanosoma evansi. There was little epidemiological information on the prevalence of infection. The present study was conducted to find out the prevalence of Trypanosorna evansi in camels through haematocrit centrifugation technique (HCT) and polymerase chain reaction (PCR). A total number of 100 camels of different age and sex groups were selected from different localities including Bahawalpur, Lahore, Gujranwala and faisalabad to find out the prevalence of Trypanosomiasis in Punjab (Pakistan) and to evaluate the sensitivity of PCR assay and HCT for the diagnosis Trypanosoma evansi. Blood samples were collected and examined by haematocrit centrifugation technique (HCT) and polymerase chain reaction (PCR). The prevalence was recorded as 4% and 13% by haematocrit centrifugation technique (HCT) and polymerase chain reaction. The positive samples by the polymerase chain reaction also included the positive animals by the haermatocrit centrifugation technique.
The results showed that PCR was more sensitive method for the detection of trypanosomiasis as compared to the haematocrit centrifugation technique. Thus PCR can be used for the diagnosis of camel trypanosornosis during both acute and chronic phases of infection, and for use in the evaluation of treatment. Application of PCR to field diagnosis is therefore clearly indicated.
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