Pigeon belongs to the avian family, Columbidae with in the order Columbiformes.
They are strong kept by the humans for variety of purposes such as food, hobby(racing) and
treatment of various diseases. However, pigeons are susceptible to a variety of diseases, but
viral diseases are more common. Newcastle disease (ND) is an acute, highly contagious viral
disease in birds, which can cause high level (up to 100%) mortality in chickens, the most
important natural host of the disease, but it can also affect a wide variety of avian species,
causing severe disease.
This disease is endemic in Pakistan and has a huge impact on the poultry industry.
Besides vaccination if hyper immune yolk is properly harvested and purified it can be used in
field condition for the treatment of infected birds .Therefore this study had been conducted to
check the effectiveness of hyper immune yolk in treatment of experimentally infected
birds.Suitability in usage of hyperimmune yolk in field condition aginst NewCastle
Disease.In order to get the hyper immune yolk 10 eggs from Big Bird hatchery were
collected.Antibody titer against NDV in egg yolk was determined by Haemagglutination
inhibition test HI their titer was 1:512 which was significant for trial.Birds were separated
into five groups A, B, C, D and E. They were challenged with NDV and treated with hyper
immune yolk in different concentration.Group A was given hyper immune yolk first then
after 24 hour NDV was given, Antibody titers at different days were calculated .Geometric
Mean Titer (GMT) values of Heamagglutination Inhibition (HI) test against NDV at day 0,
10, and 22 were log2
`2.23, log2 3.23, and log2 3.18 respectively.Group B hyper immune yolk
and NDV were given simultaneously in group the GMT values of Heamagglutination
Inhibition (HI) test against NDV at day 0, 10, and 22 were log2 log2 2.35, and log2 3.19 an
log2 3.22 respectively.Group C NDV was given first then after 24 hour hyper immune was
Summary
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injected intramuscularly. Geometric Mean Titer (GMT) values of Haemagglutination
Inhibition (HI) test against NDV at day 0, 10, and 22were log2, log2 2.47, and log2 2.99 and
log23.16 respectively.In Group D NDV given first then after signs and symptoms hyper
immune yolk was injected.GMT values of Heamagglutination Inhibition (HI) test against
NDV at day 0, 10, and 22 were log2 2.30 log2 2.83, and log2 3.06 respectively was
calculated.Group E was kept as positive control it was infected with NDV only the GMT
values of Heamagglutination Inhibition (HI) test against NDV at day 0, 10, and 22 were
log22.13 log2 0.00, and log2 0.00 respectively was calculated. The hyper immune yolk
containing 32 units against NewCastle Disease virus was injected in group A and B induced
80% protection.The yolk containing 64 units of antibodies was given in the group C resulted
into 60% protection.Hyper immune yolk consisting 128 units of antibodies injected in the
group D,in which clinical sign and symptoms of disease were shown given 60% protection
against the New Castle Disease. From this experiment it is inferred that usage of 128 units of
antibodies can induce protection against NDV infected birds and 32 and 64 units of HIY can
be used as prophylactic measure.