51.
The Incidence And Pathology Of Neoplasms Of Cattle In District Mirpur Azad Kashmir
by Ahmad Rafi | Dr. Shakeel Akhtar Khan | Dr shahzada khurram Ashraf | Dr. Mohammad | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1993Dissertation note: A survey was conducted in Bhimber, Sammani and Iftikharabad areas of District Mirpur, Azad Kashmir to record the incidence of neoplasms among the cattle. One hundred and thirty tissue specimens (suspected neoplasms) were obtained either by biopsy or from slaughter houses. The tissue specimens were examined macroscopically and microscopically.
Out of total tissue specimens, 100 samples (43 biopsy samples and 57 morbid samples) proved neoplasms. Neoplasms accounted for 74 malignant and 26 benign. These neoplasms included 34% Bovine Ocular Squamous Cell Carcinoma, 16% I-Torn Cancer, 6% Carcinoma, 2% Adenocarcinoma, 16% Lymphosarcoma, 14% Papilloma, 6% Melanoma and 6% Fibroma.
Availability: Items available for loan: UVAS Library [Call number: 0366,T] (1).
52.
Studies On Contribution Of Sheep & Goats In The Epidemiology Of Haemorrhagic Septicaemia
by Masood Anjum | Dr. Muhammad Amin Sheikh | Dr. Haji Ahmad | Dr. Muhammad Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Nature of contents: ; Literary form: Publisher: 1994Dissertation note: A total of 500 animals (sheep 250, goat 250), of both sexes and different age groups were examined for harbouring Pasteurella multocida in their respiratory tracts. The samples from live animals were collected by sterile cotton swabs, gently passed in their nasal cavity. The slaughtered animal's laryngotracheal region were directly approached for collection of samples. Lungs showing pathological lesions were collected from fifty animals each of both species. Tryptic soya broth was used for primary isolation of organism.
Only one goat yielded Pasteurella multocida which proved highly virulent both for rabbits and mice. The isolate fermented glucose, fructose, mannose, mannitol, sorbitol and saccharose producing acid only. A negative reaction was observed for arabinose, dulcitol, maltose, lactose, raffinose and salacin. The organism was positive for catalase, oxidase, indole production, nitrate reduction and hydrogen sulphide production tests. It gave a negative reaction for methyl red, voges proskauer, urease activity and gelatin liquefaction tests. The organism was found non-motile in hanging drop preparation.
The organism was highly sensitive to ampiclox, cefazolin and velosef, moderately sensitive to cephalexin, slightly sensitive to tobramycin, cloxacillin and resistant to lincocin, erythrocin, amoxydillin, doxycillin and sulpha-methaxazol trimethoprim.
Amongst the various disinfectants tested, the organism showed maximum susceptibility to sanitizer and poulphene, moderate sensitivity to saniguard and sanitec and resistance to phenyl, sanitol and phenol.
The organism in infected faecal matter maintained its viability for 9 days in shady place, sunlight and at room temperature. At a controlled temperature of 32°C. with 75-80% humidity, the organism remained alive for 10 days.
Availability: Items available for loan: UVAS Library [Call number: 0373,T] (1).
53.
Efficacy Of Triclabendazole Against Fascioliasis In Sheep And Goat Under The Field Conditions
by Haleem Hasan, Shah | Prof.Dr.Muhammad Ashraf | Dr. Haji Ahmad | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0374,T] (1).
54.
Comparative Efficacy Of Nitrofurans, Sulphadimidine & Monensin Against Coccidiosis In Sheep & Goats Under Field Conditions
by Anselm Lewis, Khokhar | Prof.Dr.Muhammad Ashraf | Dr. Haji Ahmad | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1993Dissertation note: The present work was designed to study the comparative efficacy of Nitrosol, Suiphadimidine and Monensin against naturally infected sheep and goats with coccidiosis. Forty sheep and forty goats (infected) were selected and divided into four groups each i.e. S1, 2, S3, 84 and G, 02, G, 04 respectively. The animals of groups S and were treated with nitrosol 15 mg/kg body weight, S2 and 02 with sulphadimidine 140 mg/kg body weight, S3 and G with monensin 5 mg/kg body weight daily for five days. The sheep and goats in groups S4 and G4 were not medicated and served as control. The oocyst count per gram of faeces were compared on the 7th, 15th and 21st day of post medication.
The oocyst count was reduced in groups S, S2, and S3 by 95.79%, 99.53% and 85.81% and in groups 1, G by 95.97%, 99.60% and 85.01%. The oocyst count in infected untreated control groups S4 and 04 increased.
All the three anticoccidials tested gave appreciable response in reduction of oocyst counts but suiphadimidine was found to be the best amongst the three drugs. Nitrosol proved to be the 2nd whereas monensin proved to be the third in efficacy against coccidiosis in sheep and goats. No side affects of these drugs were observed) it is suggested that further research should be planned to test the efficacy of other anticoccidial drugs like lasalocid, amprolium colpidol, robenidine coxistac etc. under local conditions to select new drugs against coccidiosis in sheep and goats.
Availability: Items available for loan: UVAS Library [Call number: 0376,T] (1).
55.
Experimental Induction Of Coccidiosis In Broiler Chicks With Eimeria Tenella And Comparative Efficacy Of Different
by Noora Jan Issot | Dr. Hajid Ahmad Hashmi | Dr. Muhammad | Dr. Muhammad Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1994Dissertation note: The study was designed to look into the immunogenic properties of Eimeria tenella. Two hundred and twenty five day old broiler chickens were reared under controlled conditions. They were equally divided into five groups i.e. A, B, C, D, and E. Group 'A' was kept as uninfected control. Group 'B' acted as infected control, which was infected with 50,000 sporulated oocysts at day 15, 25 and 35 of age. Group 'C' acted as infected and treated, which received sporulated oocysts on day 15, 25 and 35 alongwith 5 days treatment with salinomycine (60 ppm in finished feed) after 3 days of each primary and challenge dose. To group 'D' 1000 live sporulated oocysts were administered orally at day 3 and 10. Similarly, 'E' group received formalin treated 1000 sporulated oocysts orally at day 3 and 10 of age of chickens. Subsequently the members of group D and E received challenge infections of 50,000 Eimeria tenella sporulated oocysts at day 15, 25 and 35.
Immunogenicity was measured by the passage of oocysts per gm of faeces and by comparative weight gains amongst different groups. It was noticed that no coccidiosis could be established in groups A, C and E and the OPG counts remained nil throughout the period of experimnt. Group B showed high counts and group 'D' showed moderate ounts but towards the completion of experiment the OPG counts gradually reduced to 8400 and 6100 respectively, in both group.
Weight gains recorded were 473, 393, 316 and 310 gin. greater in A, E, D, and C respectively, as compared with group B. Group E depicted 1OC% protection and remained second to the uninfected control group A in respect of weight gains. Group 'D', although developed infection but due to acquired immunity became resistant to challenge infections and was placed 3rd in weight gain. Inspite of complete protection in group C due to Salinomycine treatment, the weight gains remained lower than group D and E.
Availability: Items available for loan: UVAS Library [Call number: 0381,T] (1).
56.
Antiseperim Antibodies: A Plausible Cause Of Repeat Breeding In Nili-Ravi Buffaloes
by Amir Saeed, M | Prof. Dr. Muhammad Aleem Bhatti | Prof. Dr. Rashid Ahmad Chaudhary | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1994Dissertation note: The present study was designed with the assumption that high titres of antisperm antibodies in repeat breeders might be a contributing factor for conception failure in such animals. For this purpose, 32 buffaloes/buffalo heifers were divided into four equal groups. From the normal cyclic and repeat breeder buffaloes, 48 serum samples were collected at 0, 1, and 24 hours after insemination, respectively. Oestrus mucus was also collected from these animals. A total of 16 serum samples were collected from virgin heifers and pregnant buffaloes. The serum and mucus samples were subjected to simple agglutination test, sperm quantitative penetration test and sperm cervical mucus contact test. High antibody titres were recorded in the serum and oestrus mucus of repeat breeders. Moreover, it was observed that lesser number of spermatozoa penetrated through a definite distance in cervical mucus of repeat breeder buffaloes as compared to cervical mucus of normal cyclic animals. Similarly higher number of spermatozoa exhibited shaking movement over a period of time in cervical mucus of repeat breeders, which indicates the presence of spermagglutinins.
Thus, it can be concluded that antisperm antibodies may be responsible for conception failure in repeat breeder buffaloes.
Availability: Items available for loan: UVAS Library [Call number: 0383,T] (1).
57.
Taxonomical Studies Of Endo Parasites & Their Sequence Of Prevalence At Different Ages Of Layers
by Anwar ul Haq | Dr. Hajid Ahmad Hashmi | Dr. Mubasher Saeed Mian | Dr. Sh | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1994Dissertation note: In the present study the total infection of gastro-intestinal parasites were studied in commercial layers, maintained at various poultry farms located in and around Lahore. About 930 faecal samples were collected from those birds which were dewormed at least one month before. Birds were divided into six age groups starting from Week-i to 48.
Out of 930 faecal samples, 689 (74%) were positive for parasitic infection and 241 (26%) were negative. Ascariclia g.1ij was found in 432 (46.4%) faecal samples, Heterakis gallinarum 326 (35%) and Coccidiosis in 237 (25.4%) of the total faecal samples examined.
The age wise incidence of parasites in group A (1-8 wks) was 59.3%, in group B (9-16 wks) 66.04%, iii group C (17-24 wks) 74.1%, in group D (25-32 wks) 74.8%, in group E (33-40 wks) 88.3% and in group F (41-48 wks) 81.2% i.e. ascending order of infection. In case of seasonal prevalence of infection, the occurrence of Nematodes in June was 41.25%, in July 40.25%, in August 45.75% and in September 38.80%. The incidence of Coccidiosis in June was 27.7%, in July 24.8%, in August 16.1% and in September 28.5%. The results were recorded on the basis of E.P.G. (Egg Per Gram) of nematodes and O.P.G. (Oocyst Per Gram) of Eimeria species. In general low body weight gain and low Egg production (30-40%) in the parasitised birds as compared with non-parasitised birds were noted. Statistical analysis revealed non-significant difference between prevalence of different species of parasites in the birds.
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58.
Effect Of Substitution Of Fish Meal With Cotton Seed Meal On Performence Of Broiler Chicks
by Umer Malik, M | Dr. Nisar Ahmad | Dr M. Javed Qurshi | Dr. M. Aslam.
Material type: ; Format:
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Publisher: 1994Dissertation note: The present study was under taken to investigate the effect of substitution of fish meal which is comparatively costly protein supplement with cheaper cotton seed meal on performance of broiler chicks. As one hundred and eighty Star Bro day old broiler chicks were reared on five experimental rations upto 50 day of age. The control ration contained 12 % fish meal (100 %) which was replaced by the cotton seed meal at 0, 4.17, 7.39, 11.03 and 14.72 % level (0, 25, 50, 75 and 100% on Protein equivalent basis). The starter rations were fed upto 28 days of age and finisher rations were fed upto 50th day.
Results revealed however non significant difference among weight gain of chicks fed on different experimental starter rations. But significant differences among weight gain of chicks fed on finisher rations were noticed.
The chicks fed on rations containing 100%,75%, 50% and 25% fish meal were heavier as compared to those chicks fed on rations in which fish meal was replaced by cotton seed meal on protein equivalent basis. Non significant differences in total weight gain were noticed amongst chicks fed on different experimental rations. highly significantly more feed was consumed by the chicks fed on starter and finisher rations containing 100%, 75%, and 50% fish meal as compared to those chicks fed on other experimental rations. Apparently better feed utilization in chicks were noticed by addition of cotton seed meal at different levels i.e 4.17, 7.39 and 11.03 % in the starter and finisher rations. However non significant differences among the feed efficiency values were observed in chicks fed on various experimental rations. However dressing % of all the birds fed on different rations was found non significant. Present study indicated that addition of cotton seed meal did not significantly effect the growth rate, however, slightly decreased growth rate was observed with progressive increase of cotton seed meal in the ration. Feed efficiency was comparable with that of fish meal. Results also indicated that ration containing cotton seed meal were comparatively cheaper and cost per Kg of live weight was decreased. Therefore, net profit per chick was greater.
Availability: Items available for loan: UVAS Library [Call number: 0394,T] (1).
59.
Influence Of Varying Levels Of Proten & Energy On The Performance Of Broiler Chicks
by Safiq ur Rehman | Dr. Nisar Ahmad | Dr. Ehtisham | Dr. Jawad Ahmad Qureshi.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1994Dissertation note: A study was undertaken to investigate the influence of varying levels of protein and energy on the performance of broiler chicks. The performance of chicks were studied under the heading of weight gain, feed consumption, feed efficiency, dressing percentage, organs weight, abdominal fat and economics.
Nine experimental rations containing metabolizable energy 2600, 2900 and 3200 Kcal ME/kg with 18, 20 and 22% crude protein were tried on two hundred and seventy day old broiler chicks of both sexes for the period of 50 days.
The results showed that the ration containing 20% C.P. and 2600 KCal ME/kg were best in promoting weight gain, feed utilization and were economical as compared to other experimental rations.
Different energy-protein levels did not influenced the dressing percentage and organs weight, but the diet containing high energy resulted significantly more deposition of the abdominal fat.
It was concluded that low energy and high protein combination in poultry feed formulation improved the growth rate, feed efficiency, feed conversion ratio and could be economical as compared to the high energy and high protein ration.
Availability: Items available for loan: UVAS Library [Call number: 0395,T] (1).
60.
Effect Of Vitamin A Deficiency And Excess On Immune System Of Broiler Chickens
by Ahmad Raza | Dr. Shakil Akhtar Khan | Dr. Haji Ahmad | Dr. Shahzada Khurram Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1994Dissertation note: This study was conducted to investigate the effect of different dietary levels of vitamin A (Excess, deficient and recommended) on body weight gain, lymphoid organs, haematological values, humoral response to NDV-vaccination, cellular response to tuberculin and phagocytic activity of neutrophils of broiler chickens.
The results of the study revealed that vitamin A supplementation at 20,000 1U/Kg feed had beneficial effects on the weight gains of birds; weight of lymphoid organs such as bursa of Fabricius, spleen, thymus and liver; serum antibody development against NDV vaccination; feed intake and feed conversion ratio; phagocytic index and delayed hypersensitivity index. Whereas, vitamin A deficiency had adverse effects on body weight gains; weight of lymphoid organs, serum antibody development agaist NDV vaccination, feed intake and feed conversion ratio, phagocytic index and hypersensitivity index.
No significant effect of vitamin A supplementationldeficiency was noted on haematological values.
From the results of this study it can be concluded that vitamin A is not an immunosuppressive agent when used at recommended level or even in excess (for short duration). Vitamin A deficient level in diet had detrimental effects on immune system in terms of lowered humoral and cellular responses and also decreaed phagocytic activity of neutrophils. Whereas, vitamin A supplementation at excess level improves cellular and humoral responses along with improved phagocy bic activity of neutrophils.
Availability: Items available for loan: UVAS Library [Call number: 0398,T] (1).
61.
Studies On The Use Of Sesame Meal (Punjnb Til 90) As A Vegetable Protein Supplement In Broiler Rations
by Safdar Mahmood Saeed | Dr. Nisar Ahmad Supervisor | Dr. Javaid Qureshi Member.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0399,T] (1).
62.
A Study On The Effect Of Organo Phosphate Insecticide (Neguvon) On Immune Response In Broiler Chickens
by Shoaib Ahmad | Dr. Muhammad Naeem | Dr. Muhammad Naeem | Dr. Shakil | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1994Dissertation note: Neguvon, an Organophosphate insecticide at a dose of 50mg or 100mg per kg of feed for varrying period of time administered to the birds, interfered with the development of serum antibodies in vaccinated and challenged birds. Neguvon had adverse effects on weights of lymphoid organs such as bursa of fabricius, spleen and thymus. It decreased the live body weights gain and dressed eviserated weights of birds. Neguvon had not beneficial effects on serum antibodies development. It resulted in higher postchallenged mortality of medicated vaccinated birds as compared to vaccinated control birds. So Neguvon is immunosuppressive insecticide when fed at subtoxic dosage levels.
Availability: Items available for loan: UVAS Library [Call number: 0400,T] (1).
63.
A Study On The Prevalence Of Mange In Camels And Its Effects On Some Blood Parameters During Winter Months
by Bashir Ahmad | Dr. Muhammad Sarwar Khan | Dr. Khalid | Dr. Muhammad Athar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1993Dissertation note: Camel is a versatile creature of the Nature. Its population in Pakistan is 9,58,047 heads. Its importance is obvious from the facilities which it provides to the mankind e.g. transportation and ploughing facilities, lifting of water and pulling of carts, provision of milk meat and earning of foreign exchange. But. on the other hand, camel is ignored in provision of better managernental facilities due to which it is facing many disease problems. Similarly, camel is also ignored by our scientists and a little research work has been done on camel in the world.
Keeping in view all these factors, this project was designed to see the prevalence of mange in camel. This study also include haematological changes in camel blood suffering from mange.
This study was conducted during winter months of the 1992 at Lahore abattoir. For the prevalence of mange, 150 camels suspected for mange were selected and their skin scrapings and blood was collected.
Skin scraping was examined with the help of microscope at Laboratory of Medicine Section, College of Veterinary Sciences, Lahore. During the study 19 camels out of 150 were found positive to mange and this is calculated as 12.66%.
Regarding the 2nd portion of the study, blood of 19 mange infected cases were analysed for total leukocytic count (TLC), differential leukocytic count (DLC) and erythrocytic sedimentation rate (ESR). At the same time blood of 19 mange free camels was also examined and it was found that due to mange infection, TLC values were increased. In case of DLC, Eiosinophils and lymphocytes showed increase in their number. ESR was also increased due to infection of mange in camels.
Availability: Items available for loan: UVAS Library [Call number: 0403,T] (1).
64.
The Host Reaction To Experimentally Induced Eimeria Infections In Broilers Chicks
by Ata ur Rehman Awan | Dr. Haji Ahmad Hashmi | Dr. Mohammad | Dr. Mubasher Saeed Mian | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: The experiment was designed to study the host reaction to the experimentally induced Eimeria species in broiler chicks. For this purpose two hundred and twenty, one-day-old chicks were reared. At the age of day 3, they were divided randomly in four groups each comprising of 55 birds. They were named as A, B, C and D. The group A was kept as un-infected control, while group B, C and D, at the age of day 3, received a primary dose of 1000 virulent sporulated oocysts, through oral, intraperitoneal and subcutaneous routes respectively. The challenge doses of 50,000 virulent sporulated oocysts each were adminigtered to the members of each group B, C and D on days 13, 28 and 43 of age.
The OPG counts in group A remained nil throughout the experiment the maximum OPG counts 65,500 were recorded in group B by the day 21. On the same day OPG counts of group C and D were also on the peak found to be 40,500 and 54,500 respectively. Three mortalities occurred in group B, 4 in C and 6 in group D. Similarly the maximum weight was attained by the members of group A (Control) i.e. 1750 gm, secondly by group B (oral) i.e. 1530 gm, thirdly group D (S/C) i.e. 1410 gm and fourthly group C (I/P) i.e. 1280gm. The difference in B was of 220gm, in C 470 gm, and in D 340 gm lesser as compared to group A.
Availability: Items available for loan: UVAS Library [Call number: 0404,T] (1).
65.
Study On The Pathlogy Of Leg Deformity In Broiler Chickens
by Farrukh Khurshid | Dr. Shakil Akhtar Khan | Dr. Haji Ahmad Hashmi | Dr. Shirin Munir | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: The project was designed to. study the pathology of legdeformity in broiler chickens. Fifty leg deformed broiler chickens were collected from 30 commercial poultry farms of Lahore, Kasur and Pattoki. Abnormal posture or gait, reluctanceto walk and twisted, bent, bowed or rotated legs were the presenting signs. Routine post-mortem examination was performed to observe qualitative (severity and direction of deformity, position of gastrocnemius tendon, subjective assessment of bone strength, swollen and enlarged joints) assessment of bones. Four cases of varus, 30 cases of valgus, 14 cases of slipped tendon, 2 cases of dislocated condyles were recorded, in gross pathology 30 cases of dyschondroplasia, 4 cases of rickets, 7 cases of thickening of tendons, 1:3 cases of synovitis, 5 cases of femoral head necrosis, 1 case of bone abcess and 8 cases of curly toes were observed. It was observed that male birds had greater incidence of leg deformity than females Hubbard strain had greater incidence than other strain and It was noted that the most important age of deformity was 2nd and 3rd week of age and Hubbard birds grew fastly at this age and more prone to leg deformity. The affected portions of bones and tendons were processed for histopathological examination.
Availability: Items available for loan: UVAS Library [Call number: 0408,T] (1).
66.
Comparative Evaluation Of Maize, Wheat And Brokern Rice As A Source Of Energy In Broiler Diets
by Shabbir Ahmad | Prof.Dr.Talat Naseer Pasha | Dr. Jaweeed Ahmad Qureshi | Prof. Dr. Rashid.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1995Dissertation note: Maize, broken rice and wheat are the major energy source being used in poultry diets. The energy contents in these grains are acertained according to the NRC (USA) or ARC (UK) recommendations. It was noticed by the nutritionists of poultry feed industry of the country that these assumptions does not coincide with our indigenous grains. The present study was designed to evaluate the energy contents of these main cereals through a biological trials.
For the study 240 day old chicks were perchased and randomely divided into six groups of 40 birds each and further subdivided into 4 replicate of 10 birds each. Six starter rations containing a conventional ration A (Maize+Rice+Wheat), Ration B (yellow maize), Ration C (broken rice), Ration D (wheat), Ration E (yellow maize+broken rice) and Ration F (wheat+broken rice) were formulated and fed from 0-28 days. All the diets were isonitrogenous (CP 220%) and isocaloric (ME 3000 kcal/kg). Similarly 6 finisher diets containing, a conventional finisher dietA1 (maize+rice+wheat) Ration B1 (maize), Ration C1 (broken rice), D1 (wheat), Ration E1 (rnaize+broken rice) and Ration F1 (wheat+broken rice) were formulated. Finisher diet was fed from 29- 40th days of age. Finisher diets were also isonitrogenous (CR 19.00) and isocaloric (ME 3100 kcallkg).
In the experiment it was assumed that ME value of wheat and broker rice is equal to that of yellow maize, i.e. 3400 kcal/kg.
At the end of experiment (40 days) for rations AA1 (conventional), RB1 (maize), CC, (rice), DD, (wheat), EE1 (maize+rice) and FF, (wheat+rice) average liveweight gain were 1651, 1708, 1900, 1723, 1844 and 1757 gms and feed efficiency ratio was 1.828, 1.845, 1723, 2.028, 1.67 and 1.862, respectively. The results indicated signilicant difference (P<0.01) among the groups in feed consumption weight gain (P<0.05) and feed efficiency (P<0.01). Rice containing ration (CC) was 6.6% more efficient than maize (BB1) whereas, wheat containing (DD1) was 9.9% less efficient that of maize (BB1) based ration. These variation may be due to difference in availability of metabolizable energy (ME) contents of these cereals. The results revealed that if the ME value of maize is 3400 kcal/kg in comparison of it ME value of rice and wheat may be 3624 kcal/kg and 3064 kcal/kg, respectively.
Economic analysis revealed that ration CC, (Rice) was the most efficient than other rations. The production of one kg liveweight for ration CC1(rice) is Rs.2.02 less, as compared to ration BB1 (maize based) and Rs.2.73 less as compared to ration DD1 (wheat) However the dressing percentage of the chicks was not different (P>0.50) among different groups.
Availability: Items available for loan: UVAS Library [Call number: 0414,T] (1).
67.
Studies On The Use Of Brewer'S Dried Yeast (Saccharomyces Yeast) As Vegetable Protein Source In Broiler
by Zahid Hussain | Dr. Nisar Ahmad | Dr. Jawad Ahmad Qureshi | Dr. M. Aslam.
Material type: ; Format:
print
Publisher: 1994Dissertation note: As about 60% of the total cost of poultry production incurs on feed, therefore, it is the major component, which mostly affects the economical poultry production. Unfortunately the poultry industry of Pakistan is mainly affected by substandard poultry feeding, which is due to limited availability of quality feed resources particularly protein sources. The incorporation of various non- conventional vegetable protein sources in poultry feed, if properly added have given satisfactory results.
Keeping in view a study was conducted to determine the nutritional value of Brewer's dried yeast replacing sesame meal on growth rate and feed utilization of broiler chicks.
Two hundreds and ten day old broiler chicks were randomly divided into seven groups of 30 birds each so as to have three replicate per group, each having ten chicks. The chicks were fed on seven different experimental rations A to G containing Brewer's dried yeast at 0, 5, 10, 15, 20, 25 and 30% of the ration respectively replacing sesame meal.
The results indicated that average weight gain of the chicks fed experimental ration A to G was 1203, 1175, 1175, 1166, 1138, 1127 and 1096 grams respectively. There was a proportionate depression in weight gain as the level of Brewer's dried yeast was increased. The proportionate depression in weight gain with increasing level of Brewer's dried yeast replacing sesame meal could be attributed to poor quality amino acid profile or biological value of the yeast as compared with sesame meal. It could also be attributed to certain anti-nutritional factors or high amounts of nucleic acids of yeast. The feed consumption of the chicks fed experimental ration A to G was 2516, 2484, 2526, 2502, 2436, 2501 and 2469 grams respectively. The chicks fed ration C consumed greater amount of feed, while the chicks fed ration E had the poor feed consumption. The feed consumption showed no proper pattern as was observed in case of weight gain and there was non-significant difference in feed consumption of different groups of chicks The feed conversion ratio of the chicks fed experimental rations A to C was 2.06, 2.11, 2.15, 2.15, 2.14, 2.15 and 2.15 respectively. The results indicated that the chicks fed ration A had the best feed conversion ratio of 2.06 and the feed conversion ratio was adversely affected on account of incorporation of Brewer's dried yeast. The dressed weight of the chicks fed ration A to 0 was 612, 600, 599, 593, 577, 577 and 555 grams respectively. The dressed weight showed the same trend as was observed in weight gain. The dressing percentage was 50.9, 50.1, 50.4, 51.30, 50.80, 51.20 and 50.60 percent respectively and there was hon-significant difference among difference groups. Similarly the weight of internal organs of the chicks per 100 grams of dressed weight i.e. Liver, Spleen, Pancreas, Heart and Gizzard showed non-significant difference among different groups. The economics of incorporation of Brewer's dried yeast revealed that the replacement of sesame meal with Brewer's dried yeast was economically viable.
Availability: Items available for loan: UVAS Library [Call number: 0417,T] (1).
68.
Use Of Matri As Vegetable Protein Source In Broiler Ration
by Saleh, M | Dr. Nisar Ahmed | Dr. Jaweeed Ahmad Qureshi | Dr. Mohammad.
Material type: ; Format:
print
Publisher: 1995Dissertation note: A study was undertaken to find out the appropriate level of matri (Lathyrus sativus linn) as vegetable protein source in broiler ration. Seven experimental rations containing 0, 10, 15 and 20 percent treated and untreated matri were tried on two hundred and ten day old broiler chicks for the period of 50 days. The chicks were randomly divided into seven groups of thirty chicks each. The birds reared on starter ration upto 28 days of age and on finisher ration for rest of the experimental period. The results showed that the starter ration A (control) containing no matri was best in promoting weight gain, feed consumption and feed utilization. The ration B containing 10% matri (autoclaved) gained more weight and consumed more feed and was better in feed efficiency. Finisher ration A (control) containing no matri was the best in promoting weight gain and the ration B containing 10 percent matri (autoclaved) stood second in number as for as weight gain, feed consumption and feed efficiency was concerned. At the end of the experiment dressing percentage and economics of different rations were also calculated Dressing percentage values were statistically non-significant so far as the cost per Kg live weight wasconcerned, ration B was cheaper while ration A was the cheaper while ration A was the cheapest as compared to all other rations. It was concluded that the ration containg 10% matri can safely be used in broiler ration without showing any deleterious effect on growth and feed consumption.
Availability: Items available for loan: UVAS Library [Call number: 0418,T] (1).
69.
Effect Of Routine Deworming On The Development Of Resistance Against Git Nematodes In Sheep
by Akram, M | Prof.Dr.Muhammad Ashraf | Dr. Haji Ahmad | Dr. Khalid Pervaiz | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1994Dissertation note: In this project the effect of routine deworming on GIT nematodes in sheep was studied with particular reference to the development of drug resistance. Comparison was studied by treating the flocks where routine deworming was practiced with the levamisole and albendazole and where routine deworming was not practiced with any medicine. One twenty sheep positive for GIT nematodes infection were selected. They were divided into six groups A, B, C, D, E and F comprising of 20 animal in each group. Animals of group A, B, C were selected from two different floclwhere routine deworming was practiced with levamisole and albendazole respectively. Animals of group D, E, & F were selected from unregistered private flocl where routine deworming was not practiced. Rectal faecal samples were collected before medication and at the seventh day of medication from all the six groups of sheep to count the eggs per gram of faeces and to count the number of larvae after culture under laboratory conditions.
Groups A & D were treated with the levamisole orally at the dosage rate of 7.5 mg/kg. Groups B and E were medicated with albendazole orally at the dosage rate of 5 mg/kg and groups C & F were kept as control and remained untreated. Then the efficacy of levamisole and albendazole in addition to the development of resistance against these anthelmintics was estimated.
Experimental study revealed that the levamisole showed 98.73% efficacy in the first timely dewormed flock and no any resistance was recorded whereas, 72.55% efficacy was noticed in the regularly dewormed flock, with a slight resistance. Albendazole had 97.13% efficacy in the first timely dewormed private flock and 61.24% efficacy in the regularly dewormed flock. This shows a strong evidence of resistance in the albendazole treated flock.
No side effect were observed in both the groups. Levamisole was found better dewormer with less chances of resistance. However, both drugs can be used safely to achieve control of nematode parasites in sheep.
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70.
Pathology Of Infectious Bursal Disease Following Challenge In Broiler Chicks Vaccinated With Three Different Vaccines
by Mamoon Rashid Chaudhry | Dr. Shakil Akthar Khan | Dr. Ahmad Raza | Dr. S. Ata-ur-Rehman | Faculty of Veterinary Sciences.
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Publisher: 1996Dissertation note: This project was designed to study the pathology of IBD following challenge in broiler chicks vaccinated with three different commercial imported live virus vaccines i.e. [BUR-706 (V1), D-78 (V2) and Bursine-2 (V3)]. A total number of 240 day old broiler chicks were randomly divided into eight groups, A through H, each containing 30 birds. Groups A, B and C were vaccinated with vaccines V1, V2 and V3 respectively as per schedule of the manufacturers and were challenged at 30 days of age with the local IBD strain procured from Veterinary Research Institute (VRI), Lahore intraocularly, while groups D, E, and F vaccinated with the V1, V2 and V3 were kept unchallenged. The group G nonvaccinated but challenged at 30 days of age was kept as control. The group H, which was non vaccinated and nonchallenged also kept as control.
All the birds were challenged at day 37 of experiment, lymphoid organs (bursa of Fabricius, spleen and thymus) to body weight indices were determined. These organs were also gross and histopathologically examined. Serum samples of each bird was analyzed for estimation of antibody titre by applying Agar Gel Precipitation technique. The experimental data was statistically analysed by complete randomized design.
The clinical signs like haemorrhages in thigh and pectoral muscles alongwith mucus in intestine were noted only in the group which was non-vaccinated but challenged. Similarly the bursa of Fabricius grossly appeared severely hemorrhagic and atrophied in the birds of group G. But the bursas of the birds inoculated with the intermediate vaccines V1 and V2 were also slightly atrophied. The spleen and thymus of the birds inoculated with the field isolate but non vaccinated were slightly swollen with grey foci on their surfaces.
Histopathological study of the bursa revealed an abundance of plasma cells, macrophages and necrotic areas in the medullary areas of follicles in the group G.
The spleen showed multifocal areas of liquefactive necrosis with lymphocytic depletion in the same group which was kept non vaccinated but challenged. The thymus showed no any marked histologic lesion.
All the inoculated birds were found positive for the Infectious bursal disease virus antibodies.
The statistical analysis of the lymphoid organ: body weight index of bursa showed a highly significant difference in the birds challenged but non vaccinated and the birds either vaccinated challenged or vaccinated nonchallenged and non inoculated birds. The lymphoid organ: body weight indices of spleen and thymus showed non significant difference amongst all the birds. Hence from this study it can be concluded that all three imported vaccines were some what equally efficacious in preventing clinical IBDV infection, except the little immunosuppressive effect of the two intermediate strain vaccines.
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71.
Comparative Study On The Effect Of Experimentally Produced Hydropericardium Syndrome On Some Visceral Organs of Three Different Broiler Chicken Strains
by Imtiaz Rashid Qureshi | Dr. Shakil Akhtar Khan | Dr. Haji Ahmad | Dr. Shahzada Khuram Ashara | Faculty of Veterinary Sciences.
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Publisher: 1994Dissertation note: Susceptibility, liver function , kidney function and gross and histopathological changes in different organs among different strains of broilers were studied in this experiment.
For this purpose 300 days old broiler chicks of three different strains (Hubbard,Lohmann and Indian River)were obtained from two different commecial hatcheries .Each strain was divided into two equal groups comprising of 50 birds each. These groups were further subdivided into two equal subqroups comprising of 25 birds each .Sub groups so formed were A1,A2,r3l,E2.Cl.C2,Dl,D2,El,E2,Fl and F2. Sub groups (Bl,B2,Dl,D2,Fl and F2) were challenged with HPS inoculum thiough S/C route on 21st day whereas groups (Al,A2,C1,C2,E1 and E2) were kept as control and inoculated with normal saline through S/C route on the same day. Clinical symptoms observed were depression, ruffled feathers, loose dropings, inappetance, disinclination to move and lowering down the head with beak point rested on the litter. Gross lesions observed were hydropericardium,hepatomagaly,nephritis, congestion and oedema of lungs. Microscopically necrosis, congestion, oedema, haemorrhages and leukocytic infiltration was recorded. For the estimation of liver and kidney function, tests for the estimation of total protein concentration and urea concentration in serum were carriedout which showed significant decrease (P<0.0l)in the total serum protein concentration and significant increase (P>0.0l) in urea concentration in the serum of experimentally challanged chicks.
This study revealed that highest mortality/slaughter occurred in Hubbard strain of broiler in which the increase in the level of urea concentration in the serum of inoculated group was the highest. Similarly lowest mortality/slaughter was recorded in Lohrnann strain in which the increase in the level of urea concentration in the serum of experimentally challenged chicks was the lowest. This study indicated that succeptibility directly depends upon the damage occurred to liver and kidneys.
The decrease in the level of total protein concentration in the serum was almost equal in all strains. As regards mortality this study exhibited that maximum mortality occurred in hubbard strain of broiler and lowest in lohmann strain which indicated that susceptibility for HPS varied among different strain of broilers.
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72.
Comparative Efficacy Of Anthelmintics Against Gastro Intestinal Parasites Of Buffalo Calves And Their Effects On Blood Parameters
by Tariq Humayun | Dr. Mubasher Saeed Mian | Dr. Haji Ahmad Hashmi | Dr. Muhammad | Faculty of Veterinary Sciences.
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Publisher: 1995Dissertation note: A total of 90 (60%) out of 150 fecal samples from buffalo calves of different age groups were found positive for gastrointestinal parasites. Coprological examination revealed Toxocara vitulorurn (42.22%) as single infection and 31.11% as mixed infection. Strongy.Zoides papillosus as 21.11% as single infection arid 20% mixed infection. Nematodirus spp. 1.11% as single. Haemonchus sp., 2.22% as single and 1.11% mixed infection. Honiezia sp. 3% as single and Eimeria spp., 21% as single and 13.33% as mixed infection. Intensity of infection of gastro-intestinal parasites in buffalo calves was determined as 41.1% of calves upto 500 eggs per gm of faeces, in 22.22% calves from 501 to 1000 epg, in 16.66% calves from 1001 to 1500 epg and in 20% calves greater than 1500 epg of faeces.
Comparative percentages of means of EPG counts of infected control subgroups of groups A,B and C showed increasing trend of EPG counts by day 14. However subgroup A3, showed 'I3.l% reduction, subgroup B3 showed 61.15% reduction and C) showed 72.29% reduction in epg counts on day 3 after medication with oxfendazole. Similarly on day 7 of medication, the reduction percentage were, 95.52, 89.93 and 96.38 in respective subgroups. Albendazole in subgroups A4, 84 and C4 showed reduction on day 3, by 67.08, 64.89 and 78.57 and 95.24 respectively. On day 14, both the anthelmintics showed 100% etticacy No significant change was noted in RBC, UBC counts Hb gm/mi and PCV%, however, Eosinophils and lymphocyte counts depicted siynificant increase in the infected subgroups.
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73.
Comparative Study On The Incidence And Pathology Of Morbid Conditions Of Uterus And Cervix Of Teddy And Local Non Descriptive Goats
by Yousaf Hassan Khera | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Prof. Dr. Rashid Ahmed Ch | Faculty of Veterinary Sciences.
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Publisher: 1996Dissertation note: The project was designed to study incidence and pathology of uterine and cervical maladies in two breeds (Teddy and Local non- descriptive) of goats. Three hundred uteri and cervices (150 from each breed) of goats of three age groups, 1-2 years, 2-3 years and above three years (50 in each group) were collected from Lahore slaughter house and then thoroughly examined for any gross pathological change. The organs exhibiting macropathological lesions were subjected to morphometric, histopathological and histochemical examinations.
Of the 300 uteri and cervices, 35 (11.66%) showed morbid conditions. In 150 Teddy goats, pathological conditions were observed in, 1-2 years age group 4 (8%), 2-3 years age group 7 (14%) and in above 3 years age group 8 (16%). In 150 local non-descriptive goats, gross pathological conditions were present in, 1-2 years age group 3 (6%), 2-3 years age group 6 (12%) and in above 3 years age group 7 (14%). These results showed that incidence of pathological conditions increased with the age of the goats in both the breeds and incidence was slightly higher in Teddy goats than Local non descriptive goats in the same age groups. The following eight pathological conditions namely metritis, pyometra, pigmentation, endometrial cysts, adhesions, fibrosis, hemorrhages and aplasia of uterus were found. The morphometric, gross and histopathological changes of each disease condition of uterine horns, uterine body and cervices were studied. The histochemical evidence of tissue exhibited PAS and alcian blue reactions revealing presence of carbohydrates were also studied.
Availability: Items available for loan: UVAS Library [Call number: 0440,T] (1).
74.
Adverse Effects Of Kanamycin In Dwarf Goats (Teddy Goats)
by Atta Ullah, M | Dr. Muhammad Sabir | Dr. Haji Ahmad | Dr. M. Arshad Qureshi.
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Publisher: 1995Dissertation note: Kanamycin, an aminoglycoside antibiotic is commonly employed as a therapeutic agent for the treatment of many infectious diseases.
Goat farming is considered to be more profitable than other farm animals. Especially the
Teddy goats are abundently kept in plains and mountaneous and barani, areas of Pakistan on account of their rapid growth, early maturity and higher rate of twins, triplets in this species.
An experiment was conducted to observe the influence of kanamycin sulphate on the haematologic and enzymatic activity in teddy goats.
In this experiment kanamycin sulphate was injected to the goats at the dose rate of 10 mg/kg body weight twice daily for five successive days. The blood sample were taken after one week and then once a week for four weeks of injection The sera thus collected were subjected to analytical examination.
The study indicated the significant increase in the total number of erythrocytes and leukocytes. The packed cell volume and the haemoglobin concentration did not change
significantly. The activity of alkaline phosphatase, alanine aminotransferase (OPT), aspartate aminotransferase (GOT) was also estimated'. The alkaline phosphatase activity was enhanced during the experimental period which returned to normal at the end of the experiment. The value of OPT activity increased after the first week of injection and persisted through out the experiment, althou9h it decreased in the later stages of study.
On the basis of the observations made during this study, it is suggested that kanamycin do have damaging effect on the liver which has resulted in an increased activity of ALP, OPT and GOT.
The increase in the number of erythrocytes and leukocytes during this study might be due to the increase in haemopiosis, resulting in the production of immature blood cells.
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75.
A Study Of Gastro Intestinal Helminthiasis And The Effects Of Natural Infection On Various Blood Parameters In The Buffaloes
by Ishtiaq Ahmad | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
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Publisher: 1995Dissertation note: A total of 76 buffaloes brought to the Lahore Metropolitan Slaughter house were studied for helminthiasis. Out of which 50 (65.79%) were found positive.
The helminthiasis positive guts were examined for the presence of helminths and the following species were identified Haemonchus contortus (11.84%), Haemonchus placei (10.53%), Ostertagia ostertagi (7.89%), Trichostrongylus axei (3.95%), Mecistocirrus digitatus (2.63%), Trichostrongylus colubriformis (7.89%), Cooperia oncophora (9.21%), Cooperia punctata (5.26%), Cooperia pectinata (6.58%) , Nematodirus helvetianus (2.63%), Bunostomum phiebotomum (2.63%), Strongyloides papillosus (2.63%), Oesophagostornum radiatum (3.95%), Trichuris globulosa (1.32%), Paramphistomum cervi (3.95%), Paramphistomum microbothrium (2.63%) and Monezia benedeni (2.63%)
The effect of helminthiasis on blood parameters like, total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration was investigated and the values recorded were 5.840±0.110x106 Cimm, 5.447±0.074x103 Cumm, 32,858±0.230%, 38.004±0.217 mm/hr. and 10.368±0.096 gm/lOOml respectively. The differential leucocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
43.16±0.531%, 43.82±0.718%, 6.40±0.187%, 5.620±0.241% and 1.040±0.124% respectively.
Similarly the helminth free (control> animals studied for their total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration, gave their averages as 7.382±0.068x106 Cmm, 6.321±0.084x103 Cimm, 38.927±0.286%, 28.213±0.148 mm/hr. and 11.892±0.286 gm/lOOml respectively. The differential leukocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
29.15±0.365%, 56.0±0.513%, 8.12410.28%, 5. 852410 .264% and 0.90±0.191% respectively.
The average values for helminth infected and helminth free animals were analysed statistically. The infected animals had a significantly decreased total erythrocytic count, total leukocytic count, packed cell volume, haemoglobin concentration, lymphocytes and eosinophils with increased erythrocytic sedimentation rate and neutrophils, while no significant difference was seen in monocytes and basophils.
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76.
The Pathology Of Experimental Vitamin A Exess And Deficiency In Broiler Chickens
by Sami Itrat, M | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Dr. Muhammad Athar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1995Dissertation note: This study was conducted on broiler chickens for 7 weeks to investigate the effect of three different dietary levels of vitamin A on different body organs (oesophagus, liver, kidneys and ureters) by studying their weight and pathological lesions. The haematological parameters (TEC, Hb estimation and PCV) were also observed.
The results of the study revealed that vitamin A supplementation at 20,000 lU/Kg feed had beneficial effects on weight of organs such as oesophagus, liver, kidneys and ureters. Whereas vitamin A deficiency had adverse effects on body organs in terms of weight and morphology. No effect of different levels of vitamin A on haematological values.
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77.
Effect Of Different Levels Of Protein And Salt On Water Metabolism In Broilers And Layer Chicks
by Faisal Gulzar Ahmad | Dr. Nisar Ahmed Mian | Dr. Jaweed Ahmed Qureshi | Dr. Muhammad.
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Publisher: 1995Dissertation note: Wet litter in poultry houses is a problem of considerable economic and pathological importance; This is due to the excessive elimination of water in the droppings and that contained in the expired air of the birds. As a result of this excessive moisture the litter becomes wet which in turn encourages the development of various diseases, unnecessary increase in labour and managemental hazards/expenses. Wet litter also encourages fly development, reduces beneficial bacterial population and creates problems in manure handling and removal.
A solution of the problem of wet litter would thus involve determination of those factors responsible for the elimination of excessive amounts of water via renal and alimentary routes. One of these causal factors is diet. The present study was designed to determine the effect of sodium chloride and certain dietary protein levels on water intake, feed consumption, moisture content of various organs as well as that of droppings.
Experiment was conducted at the department of Animal Nutrition, College of Veterinary Sciences, Lahore for a period of 8 weeks. Two hundred and seventy day- old chicks each of broiler and layer were randomly divided into six groups i.e. B1, B2 B3, B4, B5, B6 (Broilers) and L1, L2, L3, L4, L5, L6 (Layers) of 45 birds each. Each group was further subdivided into three replicates of 15 birds each. These groups were assigned randomly to six experimental rations having 20 and 23 percent crude protein and salt supplementation of 0.0, 0.75 and 1.0 percent level. Weekly feed consumption, weight gain and water intake were recorded. Moisture contents of crop, intestine, caecum, carcass as well as that of droppings were determined at the end of 4th and 8th weeks of age. Abdominal fat contents of broilers and layers were also recorded at the end of 4th and 8th weeks of age.
In broilers, the feed conversion ratio was significantly (P<0.05) affected by the level of salt supplementation. At 20 percent crude protein level, F.C.R. improved from 2.60 to 2.41 when the salt supplementation was increased from 0.0 to 0.75 percent. But the best F.C.R. (2.33) was obtained at 23 percent crud protein with 0.0 percent salt supplementation level. However, feed efficiency decreased at both the protein levels when level of salt supplementation was increased to 1.0 percent. The feed consuption within the group was not significantly (P<0.05) affected by the increase in crude protein level from 20 to 23 percent or by increae in the level of salt supplementation both for layers and broilers. In layers, the weight gain, feed consumption and F.C.R. followed a similar pattern as was seen in broilers.
Increase in the level of protein and salt supplementation in the ration increased the water intake of both the broilers and layers, though the difference was statistically non-significant. However, level of salt supplementation had a significant (P<0.O 1) effect on water intake and water-feed ratio.
At 4 weeks of age increase in level of salt supplementation at both the protein levels significantly (P<0.01) increased the faecal moisture percentage. Results of the statisical anlysis of the data at the end of 8 weeks were similar to those obtained at the end of 4 weeks.
It was observed that increased moisture contents of intestines lead to increased moisture in. the faecal material. At the end of 8th week the intestinal moisture content was significantly affected as result of increase in protein and salt levels.
The caecum is the chief site for water reabsorption in birds, and water contents of caecum have direct bearing on the faecal moisture and in turn on the wetting of litter. At 8 weeks of age, borth the protein levels did not exert a significant (P>0.05) effect of protein levels on the caecal moisture contents. Change in the caecal moisture contents as a result of increase in level of salt supplementation was also non-significant (P>O.05). Salt supplementation level significantly (P<0.01) increased the carcass moisture percentage. At the end of 8 weeks all the three factors and their interactions, except breed and salt interaction were significantly (P<0.0 1) different.
At the end of 4th and 8th weeks the abdominal fat contents did not show any significant pattern which could be correlated specifically with salt or protein levels. At the end of 4 weeks all the factors and their interactions except breed and protein interaction significantly (P<0.0 1) affected the abdominal fat contents. At the age of 8 weeks all the factors and interaction had significant (P<0.01) effect on abdominal fat contents.
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78.
Effect Of Various Dietary Antibiotics On The Performance On Broiler Chicks
by Ishaque, M | Dr. Nisar Ahmad | Dr. Ashfaq | Mr. Shahid Abbas.
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Publisher: 1994Dissertation note: A research study was conducted to find out the effect of virginiamycin, oxytetracycline and Furazolidone as a growth promoter in broiler rations.
Two hundred and forty day old broiler chicks were divided into four groups of 60 chicks each. Each group was further subdivided into three replicates of 20 chicks each.
Two experimental rations (starter and finisher) were prepared and fed to experimental chicks upto 50 days of age.
Virginiamycine, oxytetracycline and Furazolidone were mixed to experimental rations at the level of 10 mg/kg feed. The effects of these additives on weight gain, feed consumption, feed efficiency, mortality, weights of internal organs and residual effect on meat were recorded.
Feed and water were given libitum. Continuous light was provided for 24 hours.
The results revealed non-significant differences among weight gain, feed consumption and feed efficiency of the chicks fed on starter ration.
However the results obtained from 29-50 days showed significant difference (P <0.05) in weight gain, feed consumption and feed efficiency. The best weight gain was obtained with oxytetracycline supplementation followed by virginiamycine, furazolidone and the controlled group. Maximum feed was consumed by the birds fed on control ration. The best feed efficiency was noticed with birds fed on ration supplemented with oxytetracycline.
The results obtained from 0-50 days showed highly significant difference (P < 0.01) in weight gaiu of the birds. Feed consumption and feed efficiency also showed significant difference (P<0.05).
The maximum weight gain was noticed with birds fed on oxytetracycline supplementation followed by virginiamycine and furazolidone supplementation, while the least weight gain was shown by the birds fed on control ration.
Maximum feed was consumed by the birds fed on control ration and the least feed consumption was observed with birds fed on oxytetracycline supplementation followed by virginiamycine and furazolidone. The poor feed efficiency was observed with the birds fed on control ration.
Dressing percentage and weight of internal organs showed statistically non-significant.
The residual effect of antibiotics was not found in the chicken meat.
From the present study it was concluded that the addition of antibiotics in the broiler rations proved economical as compared to control.
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79.
Titration Of Infectious Bursal Disease Virus In Different Organs Of Gumboro Infected Birds And Embryonated Hen
by Saeed Anwar, M | Dr. Muhammad Naeem | Dr. Haji Ahmad | Dr. Khushi Muhammad | Faculty of Veterinary Sciences.
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Publisher: 1995Dissertation note: Infectious bursal disease virus (IBDV) was quantitated in different organs of the infected birds and in different parts of the experimentally inoculated embryonated hen eggs through agar gel precipitation technique (AGPT). The AGPT was standardized in the laboratory using known hyperimmune sera against IBDV antigen. The concentration of the agar, antibody and antigen are important factors for the development of precipitation line. Moreover, only gel containing noble agar in distilled water is important for diffusion of the antigen/antibody, while either of the other reagents such as sodium chloride, phenol crystal and sodium azide are presumably added to inhibit the microbial growth.
The serum from birds on 14 days post first bost showed 294.1 ACPT titers, while egg yolk from these bird on 30-45 days post first boost were having 68.6 AGPT titers. Postmortem examination showed the lesions
In liver,Kidney, spleen, intestine and bursa fabricius. It was observed that only bursa of fabricius showed detectable level (1:4 to 1:16) of IBD virus titers. Similarly, chorioaflantoic membrane of the inoculated egg showed 1:4 AGPT titers. From this study it was concluded that AGPT is a crude but highly reliable test for qualitative and quantitative study of IBDV antigen or antibodies.
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80.
Immune Response Of Broilers Treated With Amprolium And Chloramphenicol
by Rakhshanda Perveen Cheema | Dr. Muhammad Akram Muneer | Dr. Haji Ahmad | Dr. Muhammad Naeem | Faculty of Veterinary Sciences.
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Publisher: 1994Dissertation note: The birds that received arnprolium at recommended dosage levels had higher mean body weight than the chioramphenicol medicated, cyclophosphamide treated or untreated control birds.
Arnprolium treatment did not adversely affect the weight of bursa of Fabricius, spleen, thymus and liver of birds. Chioramphenicol treatment slightly depressed the weight of bursa of Fabricius and thymus. Cyclophosphamide treatment of birds in early life resulted in bursal atrophy and slight depression of splenic weight.
As compared to Cyclophosphamide treated and non-rnedicated control birds, the sera of NDV vaccinated birds fed Amprolium had higher antibody titres on day 42. The sera of chioramphenicol treated NDV vaccinated birds had lower antibody titres as compared to non-medicated control birds on day 42. The post-challenge sera of NDV vaccinated birds fed Amprolium also had higher antibody titre as compared to NDV vaccinated cyclophosphamide and chioramphenicol treated birds. The NDV vaccinated chioramphenicol treated and NDV vaccinated cyclophosphamide treated had high post NDV challenge mortality and the NDV vaccinated birds on amproliurn medicated rations and those on non-medicated rations did not have any significant post NDV challenge mortality.
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81.
Induction Of Oestrus In Non Cycling Nili Ravi Buffalo Heifers
by Hafeez Khan, A | Dr. Kazim Raza Chohan | Prof. Dr. Rashid Ahmad Chaudhry | Faculty of Veterinary Sciences.
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Publisher: 1994Dissertation note: Twenty one non-cycling Nili-Ravi buffalo hcifers with mean (± S.E) age of 31.71 ± 1.32 months and body weight of 417.61 ± 11.26 Kg were randomly divided into three equal groups. Animals in group A were injected 700 IU PMSG intramuscularly. Whereas, animals in group B (n=7) were treated with 1400 IU of PMSG. Animals in group C (n7) were administered 2 CC normal saline, which served as controls. Animals in oestrus were naturally bred with progeny tested bulls. Blood samples were collected before treatment, at oestrus and 10 days after oestrus for plasma progesterone estimation. Rectal palpations were done before treatment, at oestrus, 10 days after oestrus for ovarian structures and on day 60 post breeding for pregnancy diagnosis.
All the buffalo heifers in group A and B exhibited oestrus following treatment with 700 and 1400 IU of PMSG. Three animals from group C (42.85%) showed oestrus. The time from treatment to the onset of oestrus differed non significantly and was 6.14 ± 0.56, 6.00 ± 0.45 and 6.66 ± 0.47 days for groups A, B and C, respectively. No difference was observed towards expression of oestrus symptoms with varying doses of PMSG. The behavioural symptoms and physical changes i.e. frequent urination, acceptance to male, vulvalar sniffing of other females or allowing others to sniff, mucus discharge, vulvalar swelling and uterine tone were found indicative of oestrus in experimental heifers. The duration of oestrus also differed non significantly among the groups. This duration was 22.00 ± 2.01; 27.71 ± 1 .67 and 19.66 ± 0.98 hours for groups A, B and C, respectively.
Three animals from group A (n=7) conceived at induced oestrus. Whereas, no heifer from group B setteled at induced oestrus. Four animals showed next oestrus and two of them conceived. Three animals from group C exhibited oestrus and one of them conceived, rest of the animals remained non cyclic. The overall conception rates were 42.85, 28.57 and 14.28 percent for groups A, B and C, respectively.
Progesterone levels were found representative of ovarian activity in all the groups. Basal levels (> 0.5 ng/ml) of plasma progesterone were observed with smooth ovaries. Higher values for plasma progesterone ranging between 0.95 ± 0.02 to 5.68 ± 0.22 ng/ml were observed in cycling and pregnant animals reprsenting functional corpus lutuem.
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82.
Efficacy Of Various Ionophorous Antibiotics Against Coccidiosis In Quails
by Riaz Ahmad Khan | Dr. Khalid Pervez | Dr. Haji Ahmad | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
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Publisher: 1994Dissertation note: Two hundred and fifty, day old quail chicks were pruchased from a local hatchery and reared up to three weeks of age under hygienic conditions. After three weeks of age, quails were divided randomly into five groups. Group A was kept as control, group B as infected non treated, group C as monensin treated, group D as salinomycin treated and group E as suiphaquinoxalim treated.
After three weeks of age, groups B, C, D, and E were infected orally with mixed infection of avian Eimeria species, while group A was kept as control. Signs and symptoms of coccidiosis apperared after six days of infection. After apperarance of signs and symptoms the birds of group C and D were treated with monensin and salinomycin and group E treated with sulphaquinoxaline.
The effect of medication on feed consumption, weight gain, feed conversion ratio, mortality, oocyst count and dressing percentage were recorded weekly upto 6 weeks of age. There was significant difference (P<O.05) of feed intake between group B, C, D and E. Maximum feed intake was in group A, followed by group E, C, D, and B, respectively. Weight gain was maximum of group A followed by group E, C, D, and B respectively.
The feed conversion ratio (FCR) of control group A, was better than all other groups while feed conversion ratio of group B (infected nontreated) was poor than all other groups.
Oocysts count was zero on six days post of medication in group C, (monensin treated) while it was zero after eight days of treatment in group D (salinomycin treated) and oocyst count was zero after four days of medication in group E (suiphaquinoxaline treated). Oocyst count was 32.000 oocystlgm faeces in group B (infected nontreated) after 42 days of age. While it remained zero through out the experiment in control group (group A). Mortality was maximum (10%) in group B (infected non trearted) followed by in group D (4%) and group C, and group E (2%) respectively. While mortality remained zero in group A (control). Dressing percentage was better in group A (62.02%) followed by group E (59.2 1%), group C (58.45%) respectively.
Observing the effects of sulphaquinoxaline monensin and salinomycin on feed intake, weight gain, feed conversion ratio, mortality, oocysts count and dressing percentage in experimentally infected (coccidiosis) quails, it was concluded that sulphaquinoxaline has comparatively better results than monensin and salinomycin.
Availability: Items available for loan: UVAS Library [Call number: 0468,T] (1).
83.
Efficacy Of Various Chemotherapeutic Agents Against Experimentally Induced Avian Mycoplasmosis In Broiler Chicks
by Naveed Ahmad Khan Niazi | Dr. Muhammad Athar Khan | Dr. Asif | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
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Publisher: 1994Dissertation note: A total number of 200 day old broiler chicks were procured to determine the drug efficacy of tiamulin, tylosin and oxytetracycline against jylycoplasma gallisepticum infection in-vivo. The organism prior to its use was verified on the basis of morphology, staining reaction, cultural characteristics and biochemical tests. These birds were divided into 5 groups each having 40 birds. The birds of all groups were infected experimentally except group A birds. On the appearance of symptoms of CRD, group C, D and E were treated with tiamulin, tylosin and oxytetracycline respectively at the recommended doses of manufacturers for three consecutive days. The birds of group B were kept as untreated control. The efficacy of each drug was based upon morbidity percentage, mortality percentage, case fatality, clinical symptoms, feed-intake and necropsy lesions on postmortem. The tiamulin proved superior over other two drugs as only one bird died unlike tylosin-treated group D which recorded 2.5% mortality.
The oxytetracycline treated group E showed 7.5% mortality with the death of 2 birds. A total of 29 birds revealed no pathological lesion at necropsy in contrast to group E birds showing only 27 birds without necropsy lesions of CRD. The feed-intake was poorest in group E and was remained normal in both group C and D. After 144 hours post medication, the clearance of lesions were 96.6%, 90% and 76.6% for group C, D and E respectively.
Availability: Items available for loan: UVAS Library [Call number: 0469,T] (1).
84.
Helminthiasis: Its Dissemination And Treatment With Fenbendazole In The Migrating Sparrows
by Ali Razmaara, Syed | Dr. Muhammad Athar Khan | Dr. Haji Ahmad | Dr. Khalid Pervaiz | Faculty of Veterinary Sciences.
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; Nature of contents: ; Literary form: Publisher: 1995Dissertation note: The present study was conducted to bust upon the worm load in the naturally parasitized migratory sparrows, and also to determine the effect of worm load on the blood picture. Furthemore the effect of broad spectrum anthelmintic like Panacur (fenbendazole) in the experimental group in terms of eggs per gm. of faeces pre and post medication.
For this experiment 400 live migratory sparrows were purchased randomly, out of which 200 birds were slaughtered and an incidence of 64.5% parasitic fauna was found. Sparrows were found infected, with two species of nematode i.e. Ascaridia gaul was 39.5% and Heterakis gallinarum was 25% respectively. None of the bird was found infected with trematode and cestode.
The hematology of the above 64.5% parasitized sparrows was performed. The study revealed that due to nematode infection there was a slight increase in Total leucocytic count and Heterophils whereas the Total erythrocytic count, Haernoglobin level, Lymphocytes and Eosinophils were decreased which may lead to anemia, loss of growth and production.
From the left 200 wild naturally parasitized sparrows, 86 (43%) birds were found positive. These sparrows were divided into two groups one was left as control and the other group was subjected to drug efficacy. Panacur (fenbendazole) at the rate of 9.9 mg/kg body weight which was given orally. The results were observed in terms of eggs per gm. of faeces pre and post medication after 24, 48 and 96 hours respectively. The control group showed progressive increase in ova count i.e. 12.82%, 24.79% and 48.72% respectively, whereas reduction in the ova count of the medicated group was gradually observed as 83.72%, 92.25% and 95.35%.
The poultry breeders and scientists are urged to make an effort how the wild birds could mechanically transmit the parasites from one region to another. An attempt should also be made for the better understanding of bacterial and viral infections dissemination.
Availability: Items available for loan: UVAS Library [Call number: 0474,T] (1).
85.
Comparative Evaluation Of Tension Band Wire And Steel Suture For Repair Of Transversepatellar Fracture In The Dog
by Sarfaraz Ahmad, M | Dr. Muhammad Arif Khan | Dr. Mazhar Iqbal | Dr. Mubashar | Faculty of Veterinary Sciences.
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Publisher: 1994Dissertation note: The fracture of patella usually falls in a special type of fracture, which is not treated by ordinary limb and bone fixation devices. Hence emphasis is given to counteract the distracting forces and to convert them into compressive forces to achieve the desired effects. Different types of internal fixation devices have been tried in the past for the repair of avulsion fractures like Tension Band wire, compression screws, compression plates, an steel sutures.
This study was conducted on 20 mongrel dogs divided into two groups of 10 animals each. These groups were designated as group A and group B. Two methods, Tension Band wire and square steel suture were used for the repair of transverse patellar fracture in dogs.
In group A, the right patella was surgically exposed, fractured and repaired by Tension Band wire with Thomas Splint on the same limb when the operated dog started bearing weight on its operated leg, the left patella was exposed and repaired using same technique but without Thomas Splint.
In group B the right patella was repaired by square steel suture with Thomas Splint. After six weeks, the left patella was operated and repaired using same technique but without Thomas Splint. The dog of each group was kept for a period of 16 weeks after surgery. The results were obtained on the basis of their clinical and radiographic picture.
The results of this study indicate that the use of steel suture is a better fixation device for the repair of transverse patellar fracture.
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86.
Yolk And Serum: Detection Of Antibodies To Various Infectious Bronchitis Virus Strains Of Chickens
by Nadeem Ibrahim, M | Dr. Muhammad Akram Munir | Dr. Haji Ahmad | Dr. Muhammad Naeem | Faculty of Veterinary Sciences.
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Publisher: 1995Dissertation note: A total of 1318 blood samples and 290 egg samples were analyzed for the presence of antibodies to various strains of avian infectious bronchitis virus (AIBV). For this purpose broiler breeder, commercial layer, and layer breeder farms located in various geographical regions of the provinces of the Punjab and North West Frontier Province (NWFP) were visited, and flock histories in terms of production performances, growth patterns arid IB disease prevalence, were recorded) Attempts were made to sample only those farms which had experienced high morbidity, low production and mortality in the past growing or laying flocks housed at such farms. Samples from acute phase of illness and convalescent period were collected where ever possible. It was observed that there was a general trend to house and rear multiple age groups at the same time, the poultry farms were located at a very close distances from each other and were either not following any proper vaccination programmes or were not using any IBV vaccines. The seroprevalence of 1BV antibodies in unvaccinated flocks was more in areas where poultry farms were located in clusters or had developed into poultry estates In case of commercial layers, layer breeder and broiler breeder birds their misshapen and normal eggs were also collected for detection of antibodies to various strains/types of IBV. The serum and egg-yolk samples were analyzed using haernagglutination and haemagglutination-inhibition tests.This study indicated the presence of antibodies to various AIBV types such as Massachusettes-41 (M41), D-274 and D-1466. It was further observed that the breeder and commercial flocks which did not receive any killed or live IBV vaccination had significant levels of humoral and yolk antibodies indicating that various types of IBV were circulating in the poultry flocks in various areas of the Punjab and NWFP. The flocks indicating low egg production and quality; respiratory distress, and poor feed conversion ratios did suffer from the attack of some IBV strain(s) as they also had quite significant levels of humoral/yolk IBV antibodies)
Availability: Items available for loan: UVAS Library [Call number: 0477,T] (1).
87.
Comparative Study Of Growth Rate And Feed Efficiency Of Buffalo And Sahiwal Cattle On Concentrate Ratios
by Ehsan Mohsin | Prof.Dr.Talat Naseer Pasha | Dr. Nisar Ahmad Mian | Mr. Shahid.
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Publisher: 1996Dissertation note: There has been a gradual decline fn per capita availability of proteinous food particularly of animals origin for human consumption in our country. The feasible solution of the problem of meat shortage is to increase the production of meat by fattening the male buffalo calves which are available in the country. These are sold at lower rate when they are weaned. These calves can be fattened by using the rations containing wheat-straw and concentrate mixture available in the country. There is an increased competition between animal and human being for consumption of cereal grains and at the same time there is scarcity of green fodder in different areas of the country. Moreover, there are high prices of conventional energy sources in the country which limits there use in animal feeding. The present experiment was planned to determine the growth rate and feed efficiency of Sahiwal and buffalo male calves fed on different fiber (wheat straw) and concentrate ratios.
A fattening trial was conducted on four groups of 20 Sahiwal and 20 Nih Ravi buffalo male calves, having 10 calves in each group of buffalo and Sahiwal calves, fed on two different rations (A and B). The experiment was continued for 90 days. Feed consumption, weight gain, feed efficiency and economic evaluation of the rations and effect of ambient temperature and relative humidity on the performance of the calves were the parameters under study.
The results of the study revealed significant difference in weight gain and non-significant differences in feed consumption and feed efficiency. The non-significant difference for factor A and interaction but significant difference for factor B was observed during economic evaluation of the rations. The ration (with 40% wheat-straw and 60% concentrate mixture) was most economical as compare to ration A (with 20% wheat-straw and 80% concentrate mixture) in fattening, of Sahiwal and buffalo male calves.
Availability: Items available for loan: UVAS Library [Call number: 0481,T] (1).
88.
Antimicrobial Efficacy Of Different Drugs Against Experimentally Induced Salmonella Pullorum Infection In Broilers
by Ashfaq Ahmad Mirza | Dr. Muhammad Sarwar Khan | Dr. Mazhar Iqbal | Dr. Shakil | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1996Dissertation note: A total of 120 broiler chicks were randomly selected and divided into six groups viz A, B, C, D, E and F, consisting of 20 birds each. At 4 week of age, A to E groups were experimentally infected with Salmonella pullorum inoculum. Group E (infected, non-medicated) and F (non-infected and non-medicated) were kept as control. The groups A, B, C and D were treated with Ampicillin 20%, Oxy-N-50, Chioricol10 and Trimodin forte, respectively, post infection. All the groups were kept under close observation to record signs and symptoms of disease, mortality, body weight gain and Feed Conversion Ratio. Gross lesions in visceral organs were also noted during this study. Some blood parameters (TEC, TLC and Hb content) were also estimated, pre and post infection. A mortality percentage in groups A, B, C and D were recorded as, 25%, 35%, 15% and 10%, respectively, while 70% mortality was recorded in control group F. According to this trial Trimodin forte (Trimethoprim Plus Sulphadiazine) . afforded maximum protection against infection and proved best in relation to weight gain and F.C.R. Chloricol-10 (Chioramphenicol) stood second in the list, while Ampicillin-20% (Ampicillin Trihydrate) was the third drug in furnishing protection against the infection. Whereas, Oxy-N-50 (Oxytetracycline plus neomycin sulphate) was the least effective drug in respect of protection against pullorum disease. The signs of ill health appeared 3 to 4 days post infection were listlessness, ruffled feathers, droopy wings, loss of appetite, poor growth, depression, increased thirst and severe diarrhoea of chalky white colour. The postmortem findings were enlarged and congested liver streaked with haemorrhages, 2-3 times enlarged spleen (spleenomegaly), enlarged heart and pericarditis, congested and distended kidneys, area of gray hepatization in lungs, thickened and inflammed intestinal walls and presence of necrotic foci in cardiac muscles, liver, spleen, lungs, caeca, large intestine and muscles of gizzard. The Total Erythrocytic count and Haemoglobin contents were decreased and Total Leukocytic Count, were increased 3 days post infection. The findings of the present study will further elucidate the disease and help in diagnosis and treatment of this malady on large scale.
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89.
Effect Of Varying Levels Of Methionine Cystine And Lysine In The Vegetable Protein On The Performance And Growth
by Abdul Haseeb Ahmad | Dr. Nisar Ahmed | Dr. Jawad Ahmed Qureshi.
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Publisher: 1996Dissertation note: The present study was undertaken to investigate the effect of varying levels of methionine, cystine and lysine supplemented in the vegetable protein diet on the performance of broiler chicks. 180 day old chicks were reared separately on the six experimental starter and finisher rations upto 50 day of age. The starter rations had a 23% protein while the finisher rations with 20% protein. All starter and finisher rations had a similar inetabolizable energy of 3200 k cal/kg. The starter rations were fed upto 28 days of age and for the remaining 22 days finisher rations were fed.
Results revealed highly significant difference amongst wt. gain of chicks fed on different experimental starter rations. A highly significantly less weight (1859.86 grams) was gained by the chicks fed on ration "A" without supplementation of amino acids as compared to all other rations. Similarly the experimental finisher rations had non significant difference amongst the weight gain of chicks. The results of over all weight gain of all groups of chicks fed on rations A,B,C,D,E and F were 1859.86, 1881.65, 1972.44, 2008.32, 2013.20 and 2052.53 gm respectively, while the statistical analysis reveled non significant difference in weight gain of chicks fed on different experimental rations.
Highly significant difference in the feed consumption were noticed in the chicks fed on different experimental starter and finisher rations. Significantly more feed was consumed by the chicks fed on ration A, B, C, D, E as compared to ration "F".
Overall feed consumption of male chicks fed on ration A, B, C, D, E and F were 4826.30, 4735, 4563.16, 4465.36, 4381 and 4298.26 grams respectively, whereas the statistical analysis revealed highly significant difference amongst the feed consumption of chicks fed on different experimental starter and finisher rations.
Approximately better feed utilization in chicks was noticed when fed on "F" and "E" as compared to the other experimental rations A, B, C and D.
Non-significent differences between the dressing percentage, weight of internal organs and abdominal fat deposition of chicks fed on different experimental rations was observed.
The present results indicated that the male chicks can be reared economically on starter ration having 23% protein and finisher ration having 20% protein from vegetable sources alongwith 0.20% supplementation of lysine and methionine. Weight gains, feed consumption and feed efficiency was better in male chicks. The male chicks were economical to grow and cost/kg of live weight of chicks was less which was Rs.17.40/- as compared to that of control which costs Rs.20.52/-.
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90.
A Serological And Coprological Study Of Paramphistomiasis In Buffalo
by Jameel Ahmad | Dr. Khalid Pervaiz | Dr. Haji Ahmed | Dr. Muhammad Ashraf | Faculty of Veterinary Sciences.
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Publisher: 1997Dissertation note: Two hundred faecal samples, serum samples and paramphistomes used in the present study collected from main slaughter house of Lahore and from surrounding of the city.
Faecal examinations were carried out with fresh smear technique and 61.5% animals found positive for paramphistomiasis. Serum was separated from the blood samples collected from infected and suspected buffaloes for the performance of enzyme-linked immunosorbent assay. Paramphistome crude antigen was prepared from fresh stomach flukes, which contained 95.66 mg proteins/mi at 545 nm.
For performing the enzyme linked immunosorbent assay, the 96 well ELISA plates were coated with crude antigen & kept overnight & then the other procedure for the assay was followed, & the serum samples were tested with paramphistome crude antigen. In positive samples, the yellowish-brown colouration was formed.
In faecal examination, 61.5% and in the ELISA 100% of the animal found positive.
From the conclusion of the results if was found that ELISA (100%) has given more positive results then faecal examination (61.5%).
Availability: Items available for loan: UVAS Library [Call number: 0490,T] (1).
91.
Effect Of Fermacto On The Function And Morphology Of Liver And Kidneys Of Broiler Chicks
by Tahir Aziz | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Dr. Haji Ahmad Hashmi | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1997Dissertation note: This project was designed to study the effect of two levels of Fermacto feeding for different durations on general metabolic state of broiler chickens by determining the functional and morphological state of liver and kidneys. One hundred and five day-old broiler chicks were randomly divided into seven groups viz A through G each containing fifteen birds. Group A was kept as control. To other six groups Fermacto feeding was done at two different levels (2 gm/kg and 4 gm/kg of feed) and for different durations. The experimental period was 42 days. Organ: body weight indices, FCR, gross and histopathological lesions of liver and kidneys, serum glucose, serum cholesterol, serum total protein and serum uric acid levels and antibody titres against Newcastle disease studied.
The statistical analysis of organ body weight index for left kidney and right kidney showed a highly significant difference between all treated and control groups. The highest mean left kidney body weight index was achieved in the birds of group E. Similarly the highest mean right kidney body weight index was obtained in the birds of group C. Organ: body weight index for liver also showed a highly significant difference between control and treated groups and the highest liver body weight index was achieved in birds of group G. Best feed conversion efficiency was shown by the birds of group B. The gross pathological study of liver and kidneys revealed some nonspecific gross lesions such as slight atrophy, little changes in colour and streaks of haemorrhages in some birds of control and experimental groups. No characteristic histopathological lesion was observed in liver and kidneys of birds except accumulation of RBCs in some birds of control and experimental groups.
The data obtained by the analysis of serum statistically indicated that the concentration of serum glucose and serum cholesterol levels were significantly increased in treated groups than that of control group. Concentration of serum total protein was increased in all treated groups except for C group. But there was no significant difference in serumuric acid levels of all treated and control groups. The data obtained by the analysis of antibody titre against Newcastle disease on zero, 14th, 28th and 42nd day of experiment showed that geometric mean HI titres of all groups carried no significant statistical difference on any of the above mentioned days.
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92.
Effect Of Different Levels And Sources Of Fish Meal On The Performance Of Broiler Chicks
by Shahid Raza | Dr. Nisar Ahmad Mian | Dr. Javed Ahmed Qureshi | Dr. M. Saleem.
Material type: ; Format:
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Publisher: 1996Dissertation note: The present study was undertaken to investigate the effect of different levels and sources of fish meals on the performance of broiler chicks. One hundred and eighty day-old hubbard chicks were randomly divided into 18 experimental groups of 10 chicks each. The chicks of two commercially available fish meals with three levels of Gwadar and Sindh fish meals were compared in this study. The chicks were assigned to 6 experimental rations in such a way that there were three groups of 10 birds each under each ration. Th starter rations were fed upto 0-28 days of age while the finisher rations from 29-42 days of age. At the end of experiment three birds from each replicate were taken at random and slaughtered to find out the dressing percentage.
The average weight gain of chicks fed on Rations A, B, and C (containing Gwadar fish neal) were 1879.2, 1770.65 and 1712.82 gms respectively. The maximum weight (1879.52 gms) was observed in ration A (containing 8 percent fish meal). The average weight gain of chicks fed on rations D, E and F (containing Sindh fish meal) were 1697.78, 1644.5 and 1677.9 gms respectively. The maximum weight gain (1697.78 gms) was observed in ration D (containing 8 percent fish meal). The average weight gain by chicks fed on two different sources of fish meals; i.e. Gwadar and Sindh were 1787.844 and 1673.411 gms respectively.
Results revealed significant differences between the two sources and non-significant difference among the various levels of fish meals for weight gain data which showed that Gwadar fish meal was better as compared to Sindh fish meal and apparently 8 percent level was better than 11 and 14 percent levels of fish meals.
The average feed consumption of chicks fed on rations A, B, and C (containing Gwadar fish meal) were 3560.1, 3540.16 and 3373.49 gms respectively. The maximum feed consumption (356b.1)was observed in ration A (containing 8 percent fish meal). The average feed consumption of chicks fed on rations D, E and F (containing Sindh fish meal) were 3535.6, 3374.3 and 3462.73 gms respectively. The maximum feed consumption (3535.56 gms) was observed in ration D (containing 8 percent fish meal). The average feed consumption by chicks fed on two different sources of fish meals i.e. Gwadar and Sindh were 1929.74 and 18&6.777 gms respectively.
Feed consumption of chicks fed on rations containing two sources of fish meal were non-significant. On the other hand there was significant differences in feed consumption of chicks fed on rations containing various levels of fish meals. Apparently better feed consumption was observed in Gwadar fish meal as compared to Sindh fish meal.
Statically non-significant difference was observed in the feed efficiency value for both different sources and various levels of fish meals. Apparently Gwadar fish meal had better feed efficiency than Sindh fish meal while in case of levels 8 percent had better feed efficiency as compared to 14 and 11 percent respectively.
Availability: Items available for loan: UVAS Library [Call number: 0501,T] (1).
93.
Changes In The Harderian Gland And Respiratory Tract Of The Broiler Chicken Following Intraocular Vaccination And Infection With/Against Newcastle Disease Virus
by Amer Jumal, M | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Dr. M. Asif Rabbani | Faculty of Veterinary Sciences.
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Publisher: 1997Dissertation note: This project was designed to determine the immune response, macroscopic and microscopic changes in the Harderian gland (HG) and respiratory organs of young chickens reared in well controlled environment following eye-drop vaccination against ND and subsequent challenge with a NDV field strain. This endeavour also represents an effort to raise the curtain on the extent of local and humoral immunity in response to eye-drop vaccination against NDV and also after challenge by two different routes (intraocular and intratracheal).For this project a total of 150 day old broiler chicks were procured from local commercial hatchery. At day one 6 birds were used to determine the maternal antibody titres against ND. On day 7, chicks were randomly subdivided into 6 subgroups Al and A2, Bi and B2, Cl and C2, each subgroup having 24 birds. Three subgroups were vaccinated through eye-dropping against NDV (i.e. Al, A2 and Cl) at 7th and 21st day of age. The other three sub-groups were kept non-vaccinated (i.e. Bi, B2 and C2). Challenge of virulent NDV was administered at 28t1i day of age by two different routes to the different subgroups. Intraocular challenge was given to Al and Bi subgroups and intratracheal challenge was given to A2 and B2 subgroups, whereas Cl and C2 subgroups were kept as control. Determination of antibody response against NDV by heamagglutination inhibition test and gross and histopathology of Harderian glands, trachea and lungs of six birds of all experimental and control subgroups were performed on days 14, 28, 31 and 35 of age. Ocular vaccination engendered higher level of circulating antibodies against NDV but developed poor local protective immunity in tracheal rrucosa. Intratracheal challenge, caused pronounced changes in vaccinateçl birds by eye-dropping in comparison to intraocular challenge, so it can be infered that route of challenge of NDV played a characteristic' role in developing disease to the host vaccinated through eye-droppings. Availability: Items available for loan: UVAS Library [Call number: 0504,T] (1).
94.
Comparative Efficacy Of Cloprostenol, Estradial And Gentamycine For The Treatment Of Endometritis In Buffaloes
by Chaudhry, Shafiq, M | Prof. Dr. Rashid Ahmad Chaudhry | Prof. Dr. Muhammad Aleem Bhatti | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1996Dissertation note: This study was designed to compare the efficacy of Cloprostenol, EstradioJ and Gentamycine for the treatment of endometritis in buffaloes.
Eighty buffaloes suffering from endometritis were selected amongst the animals brought for A.J. on repeat breeding at different A.I. Center/Sub-Centres in Lahore and Gujranwala city. After detailed gyanaecological examination animals were randomly divided into four treatment groups. Twenty buffaloes in group A were injected 500 ig of Cloprostenol (Estrumate). On day 8 of the oestrus cycle and cured animals were inseminated at the following oestrus. Twenty buffaloes in group B were given 3 intrauterine infusion of stilbestrol. On day 3, 5 and 7 of the oestrus cycle and cured animals were inseminated at the following oestrus. Twenty buffaloes in group C were given single dose of gentamycine (intrauterine infusion) on the day of the oestrus cycle and cured animals were inseminated at the following oestrus. Twenty buffaloes in group D served as control and were inseminated without giving any treatment. After two months all the animals were rectally palpated for pregnancy diagnosis. Seventy animals were tested for pregnancy diagnosis. While others were later transferred to untraceable places. The percent curative and conception rate observed in Cloprostenol treated group 'A' were 65% and 58.33% respectively similarly in group B Stilboestrol treated animals the curative rate was 75% and conception rate was 64.28%. In group 'C', the animals treated with Gentamycine, the curative and conception rate were observed 45% and 42.85%. In control group 'D' only 35.71% animals were conceived after insemination.
On the basis of curative and conception rates Stilboestrol was found better than Cloprostenol and Gentamycine for the treatment of endometritis buffaloes. The cost of treatment with stilboestrol is lower compared with Cloprostenol and Gentamycine. On the other hand a certain degree of invonvience remains with Stilboestrol therapy as intrauterine infusion demands special skill.
It is concluded that Stilbestrol and Cloprostenol results were signficanly higher as compared to treated with Gentamycine and control group.
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95.
Taxonomical Study Of Treatment Infections And Their Effect On Blood Picture In Sheep And Goats
by Sulman Hameed | Dr. Asif Rabbani | Dr. Haji Ahmad Hashmi | Dr. M. Sarwar | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1996Dissertation note: In the present study two hundred sheep and two hundred goats both above one year of age and below one year age were examined after their slaughtering at abattoirs of Lahore for the presence of Gastrointestinal trernatodes during the month of May to August, 1995.
Monthwise prevalence of p. Cervi, f... hepatica a6% D. dendriticum and D. dendriticurn during the month of May to august, 1995, was as under:
May 1995 15% cervi, 20% f hepatica 6% D. dendriticum
June // 19% P.cervi 27% f. hepatica 8% D. dendriticum
July // 33% P.cervi 40% hepatica 16% D. dendriticum
August // 28%P.cervi 32% f hepatica 13% D. dendriticurn
It was observed that the infection increased gradually and reached at peak in the month of July and then declined. An overall incidence of 64.25% of trematodes was recorded in both the species. The parasitic incidence was as . cervi 23.75%, .J hepatica (29.75%) and .. dendriticum (1 0.75). So the rate of infection was on the peak in the month of July in P.cervi, E. hepatica and . dendriticurn as shown in Figure 1. The hernatological values of sheep and goats infected with trernatodes were :total Erythrocytic count 1 2.01 3 ± 0.096 million/cubic microliter, Haemoglobin level 8.037±0.160 g/l00rnl, Packed cell volume 30.10 ± 0.326%, IErythrocytic sedimentation rate 2.71 ± 0.242 mrn/24 hours and Total Erythrocytic count 10.973 ± 0.094 million/ microliter, Haemoglobin 7.637±0.128 g/1 OOml, Packed cell volume 28.15 ± 0.316% and Erythrocyte sedimentation rate 1.94±0.246 rnm/24 hour respectively for sheep and goats. It was observed that the infection with trernatode had an adverse effect on total erylhrocyte count, haemoglobin level and packed cell volume.
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96.
Taxonomuy And Prevalence Of Eimeria Species Infecting Young Buffalo And Cattle Calves And Their Effect On Various
by Afzal, M | Dr. Muhammad Afzal | Dr. Haji Ahmad Hashmi | Dr. Shakil | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Nature of contents: ; Literary form: Publisher: 1996Dissertation note: This study was contemplated to evaluate some aspects of prevalent species including taxonomy of Eimeria infection& in young cattle and buffalo calves. In addition the effects of Eimeria infections on various haematological parameters i.e., Haemoglobin, PCV, RBC count, ESR and Differential leucocyte count were also studied. For this purpose the animals (Buffalo and Cattle calves) were divided into two groups. Firstly those having diarrhoea and secondly those which were apparently healthy. The buffalo and cattle calves were divided into four groups (A, B, C and D) on the basis of the age in days under the maximum age of 120 days. A group ranged from 1-30 days, B ranged 31- 60 days, C ranged 61-90 and D ranged 91-120 days. The haematological observations were recorded from both the groups formulated upon the basis of age as A, B, C and D in both cattle and buffalo calves and findings compared with the control groups A, B, C and D having the same age range.
Results revealed that out of 816 animals (442 cattle and 374 buffalo calves), 167 (84 cattle and 83 buffalo calves) were found to be positive (20.46%) for Eimeria species infection. Whereas 62 out of 250 (24.80%) suffering from diarrhoea and 22 out of 192 (11%) apparently healthy cattle calves showed coccidial infections. For buffaloes 63 out of 220 cases (29%) were positive from animals showing diarrhoea and 20 out of 154 (13%) were found positive for coccidiosis from apparently healthy group. Four species of Eimeria namely, Eimeria zuernii, Elmerip bovis, Eimeria cylindrica and Eimeria ellipsoidalis were found in the above mentioned ca1ves
E. zuernhi was the most prevalent species found (48%) followed by . bovis (27%), Eimeria cvlindrica (16%) and Eimeria ellipsoidalis (10%) respectively. OPG count carried out ranged from 2500-70,000 oocyst per gram of faeces.
Haematological observations showed anaemia, which was a feature of the diarrhoeic calves and not found in apparently healthy animals although some of them had been harbouring Eimeria infection. The PCV decreased proportionately with haemoglobin whereas erythrocyte count also showed decrease in positive cases. On the other hand ESR of the above referred calves increased. There was neutrophilia in the infected animals versus control animals and lymphocytes decreased in infected animals. There was no significant change in eosinophil and monocyte counts.
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97.
Study On Various Mineral Levels And Pathology Of Visceral Organs Of Broiler Chickens Affected With Leg Deformities
by Asim Aslam | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Prof. Dr. Rashid Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1996Dissertation note: This project was designed to determine the incidence of various leg disorders in commercial broiler chickens and to elucidate the effects of leg deformity on the metabolism of broiler chickens in terms of serum levels of calcium, phosphorus, sodium, potassium, chloride and also on the morphology of liver, kidneys and pancreas. Fifty leg deformed broiler chickens were collected from 38 different commercial poultry farms in and around Lahore, kasur, Sialkot and Gujranwala Districts.
Out of the observed cases of leg deformity, 18 cases of valgus, 13 cases of slipped gastrocnemius tendon, 9 cases of curly toe, 5 cases of femoral head necrosis, 3 cases of varus and only 2 cases of synovitis were reported. This data of incidence of various leg deformaties were analysed on the basis of age, sex, strain of bird and also on the basis of location of deformity. It was noted that the most frequently encountered leg deformity was valgus. The maximum incidence of valgus occurred at 5th week of age. The males have greater incidence of valgus as compared to females. Hubbard strain had greater incidence of valgus in comparison to other strains of birds.
Unilateral cases of valgus were more common than bilateral. The levels of all minerals were decreased in experimental birds as compared to control ones. The mineral levels of birds affected with valgus deformity showed that there was a highly significant difference in the levels of calcium phosphorus and sodium between the experimental and control groups. But there was a non-significant difference in the levels of potassium and chloride in both the groups. The slipped gastrocnemius tendon was noted frequently at 7th week of age, slightly more in females and all the reported cases were unilateral. Hubbard strain was found to be most liable to slipped tendon. The mineral levels of birds affected with slipped tendon showed a highly significant difference in the levels of calcium, phosphorus and potassium between the experimental and control groups. A significant difference was also noted between the experimental and control groups in terms of levels of chloride, but a non-significant difference was recorded between the experimental and control groups in case of serum sodium level. Curly toe was again recorded to its peak in birds of 7 week age, more in males and mostly bilateral. Hubbard strain was found to be the most affected one. The mineral levels of birds affected with curly toe showed a highly significant difference in the levels of calcium and phosphorus between the experimental and control groups. But a non-significant difference was noted between the two groups in cases of serum levels of sodium, chloride, and potassium. Femoral head necrosis was recorded at its highest incidence at 9th week of age, more in males, and all the recorded cases were unilateral. The strain most liable to femoral head necrosis was Indian river. The mineral levels of birds affected with femoral head necrosis showed a highly significant difference in the levels of phosphorus and potassium between experimental and control groups. A significant difference was also recorded between the experimental and control groups in terms of levels of calcium. But in the serum levels of sodium and chloride a non-significant difference was obtained between the two groups. Varus was recorded more frequently at 6 and 7 week of age, more in females, and mostly unilaterally although bilateral cases were also noted. Hubbard, Arboracres, and Lohmann strains showed more susceptible behaviour. The mineral levels of birds affected with varus showed a highly significant difference in the level of calcium. A significant difference was also recorded between the experimental and control groups in terms of levels of phosphorus and potassium, but a non-significant difference was noted in the sodium level between the two groups. Synovitis was noted mostly between 3 and 6 week of age, more or less equally between males and females. Both unilateral and bilateral cases were seen, and Hubbard and Arboracres strains were found to be more prone to synovitis. The mineral levels of birds affected with synovitis showed a significant difference in the levels of calcium and sodium in the experimental and control groups. But a non-significant difference lies between the two groups.
In all these various types of leg deformities, the macroscopic lesions like, slight enlargement of liver along with discolorization and necrotic areas and in some cases congested livers showing the lesions of perihepatitis were also noticed. The kidneys showed inflammatory signs alongwith urates deposits. A pale fibrosed pancreas showed atrophic changes. The pancreas was the most severely affected organ. In majority of the birds it was severely atrophied presenting a shrunken fibrous band like structure in the convoluted duodenal loop. The colour of these pancreases was white or off-white to pink and were firm in consistency. Microscopically cloudy swellings and severe necrosis were seen in the affected livers. Mononuclear and plasma cell infiltration were also quite evident. Leukocytic infiltration in the interstitial spaces and glomerular region were quite evident. The dilated blood vessels and haemorrhages in tissue spaces were observed. In some of the severely affected cases the glomeruli were enlarged and were less in number as compared to that of normal birds.Lesions of necrosis were also noted in some of the affected kidneys. Pancreas was observed that the acinar cells were atrophied and underwent vacuolation, degeneration and necrosis. The Zymogen granules were almost absent from acinar cells. Heterophil, leukocytic and lymphocytic infiltration were frequently encountered in affected cases. A characteristic change was an inflammatory reaction in one or more pancreatic ducts where the epithelium and fibrous tissues occluded the lumen of the ducts and led to obstruction in pancreatic drainage.
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98.
Comparative Study Of Different Techniques For Diagnosis Of Lymphoid Leucosis In Commercial Layer Chickens
by Azhar Nazir | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1997Dissertation note: This project was designed to study and compare the results of ELISA, impression smears of visceral organs, haematological (TLC and DLC) and histopathological examination for diagnosis of lymphoid leucosis in commercial layers. For this purpose a total of 90 birds were selected from different commercial layer farms, having the history of lymphoid leucosis, age ranged from 5 to 9 months and having enlarged liver and bursa of Fabricious on palpation.
After the selection of birds, their blood was obtained and serum was seprated avoiding the lysis of RBCs. The serum thus obtained was used to detect the lymphoid leucosis antigen with the help of proflock kit of ELISA by KPL USA. Ninety serum samples were put into the ELISA plate well coated with an anti-P27 antibody. The anti-P27 antibody formed a antibody antigen complex. After washing the plate, an affinity purified rabbit anti-P27 peroxidase conjugate was added to each well. The antibody antigen complex remaining from the previous step reacted with the conjugate, brief incubation period was provided and then unreacted conjugate was washed through second wash step. Substrate which contained a chromogen ABTS), was added to each well. Chrornogen colour was changed (from clear to green-blue), which occurred in the presence of peroxidase enzyme. The relative intensity of colour developed in 15 minutes (Compared to control) which is directly proportional to the quantity of P27 antigen in the sample. Now the substrate was incubated, stop solution was added to each well to terminate the reaction. The plate was read using an automatic ELISA reader, which also calculated the SP (sample to positive) values for each sample tested.
The birds having titres of 1000 or above were found positive.
Twenty birds out of total 90 were found positive by ELISA.
These ELISA positive birds were purchased from each farm and the same number of healthy birds from the same flock were subjected to organ body weight ratio and haematological examination (TLC, DLC) and histopathology of positive cases.
The total leukocytic count of the ELISA positive birds showed increase in the leukocytic count when compared with the healthy birds of the same flock. The mean values for ELISA +ve birds of flock number 1, 2, 3 and 4 were 44.93±0.40, 45.90±0.41, 45.90±0.41 and 45.04±0.63, respectively with mean TLC values of 27.32±0.23, 27.38±0.35, 27.38±0.35,27.24±0.29 of healthy birds of flock number 1, 2, 3 and 4, respectively, where the differential leukocytic count showed mild increase in lymphocytes and basophilis and a marked increase in monocyte. The mean value for lymphocytes of the ELISA +ve flock numbers 1, 2, 3 and 4 were 57.35±0.14, 57.30±0.15,57.30±0.15 and 57.76±0.44 respectively compared with the healthy birds of flock numbers 1, 2, 3 and 4 which were 56.0±0.20, 56.0±0.29, 56.20±0.29 and 57.44±0.3. The mean basophilic value for ELISA +ve flock number 1, 2, 3 and 4 were 1.61±0.60, 1.56±0.24, 1.56±0.24 and 1.48±0.37 respectively, compared with the mean value of healthy birds of flock numbers 1, 2, 3 and 4 which were 1.57±0.68, 1.48±0.68, 1.48±0.59 and 1.60±0.37, respectively.
The mean monocyte value for ELISA +ve flock were 14.50±0.13,14.50±0.10, 14.50±0.10 And 14.56±0.44 for flock number 1, 2, 3 and 4, respectively, mean value of the healthy birds were 9.19±0.57, 9.10±0.44, 9.10±0.44, 9.10±0.44 For flock numbers 1, 2, 3 and 4 respectively.
The organ body weight ratio showed a highly significant
increase in the organ body weight indices of affected liver, kidney, spleen, ovary and bursa of Fabricious.
The gross lesions mainly were great enlargement of the liver, spleen, kidney, ovary and bursa of Fabricious, with grayish white, or creamy white foci. In some organ the nodular tumor growth has also seen.
Histopathological studies of the affected birds revealed the infiltration of the lymphoblasts and other blood cells in the affected organ. Areas of necrosis at some places in affected organ were observed. The tumors cells showed the aggrigates of lymphoblast. The impression smears made from the fresh specimen of liver, spleen, kidney, ovary and bursa of Fabricious, stained with Giemsa stain, confirmed the lymphoid leucosis due to the presence of lymphoblast.
Out of 20 positive cases, liver was affected in all the cases, spleen in 18 cases, ovary in 15 cases, kidney in 11 and bursa of Fabricious in 14 cases.
It was concluded that ELISA can be used as a most reliable and effective method of diagnosis in live birds, whereas in dead birds, disease can be confirmed by the presence of lymphoblasts in impression smears of liver and spleen. Blood changes can be use as aid in early diagnosis, whereas histopathology is a time consuming procedure.
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99.
Effect Of Maternal Antibodies On Antigenic Response Of Broiler Birds To Infectious Bursal Disease (228-E strain) Virus Vaccine
by Sajid Afzal, M | Dr. Muhammad Akram Munir | Dr. Atta-ur Rehman Rizvi | Dr. Haji Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1997Dissertation note: This project was designed to study the effect of maternal antibodies on the antigenic response of birds to infectious bursal disease virus (288-E strain) vaccine and to study the duration of maternal antibody against IBDV in broiler. A total of one hundred forty chicks were randomly divided into eight groups i.e. A, B, C, 1), E, F, G and H. All the birds in groups A, B, C, U, E and F were vaccinated against IBDV. The birds in groups 0 and H were unvaccinated controls. However, the groups were IBDV vaccinated according to different schedules.
The presence of IBDV maternal antibodies was tested through agar gel precipitation test, and the maternal antibodies to IBDV were observable upto day 14 of the life of chicks.
All the IBDV vaccinated birds exhibited immune response to the vaccine and their response was detectable through AGPT. Antibodies were detectable on 7th day post vaccination and the responses increased upto 14th day post vaccination. The antibody titres were at peak in all the groups between 28th to 35th day of age.
These findings indicate that maternal antibody waned away between second and third week of life. The mean±standard error values of groups A, B, C, D, E, F, G and H on day 35, were 28.33±8.56, 41.66±12.99, 43.33±11.70, 31.66±13.21, 41.66±12.56, 45.0±14.29, 26.66±11.94 and 26.66±11.94, respectively. The serologic and challenge infection study indicates that the vaccinated birds were immune against IBDV.
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100.
Anthelmintic Efficacy Of Caesalpinia Crista, Fumaria Parviflora, Mebendazole And Ivermectin Against Haemonchus
by Saeed Ahmad | Prof.Dr.Muhammad Ashraf | Dr. Khalid Pervez, CMS (25n) | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1997Dissertation note: This study was conducted to evaluate the anthelmintic efficacy of Caesalpinia crista, Eumaria parviflora, Mebendazole and Ivermectin against Haemonchus ntortus in sheep. Anthelmintic efficacy of Casesalpinia crista at the dose level of 4 gm/kg body weight was 86.85%, Furneria parvifl at the dose level of 4 gm/kg body weight was 85.32%, Mebendazole at the dose level of 15 mg/kg body weight was 98.05% and Ivermectin at the dose level of 200 jig/kg body weight was 96.55%. There was marked decrease in EPG in all the four groups A, B, C and D treated with . crista, . p.ryjflora, Ivermectin and Mebendazole respectively, whereas an increased value of EPG was observed in group E (unmedicated control) in response to the increasing intensity of infection in sheep. Haematological study of infected animals revealed a considerable increase in Neutrophils, Monocytes, Basophils and Eosinophils while a considerable decrease in Lymphocytes, Total leukocytic count and Total serum proteins was observed.
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