1.
Serodiagnosis Of Ovine Hydatidosis
by Javaid, M | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: A study was under taken to find out the incidence of hydatidosis and to evaluate the efficacy of indirect haemagglutination (IHA) test for the confirmation of natural hydatidosis in sheep slaughtered at Lahore municipal abattoir.
Blood samples from 200 sheep (50 each from hydatidosis affected and free sheep on the basis of postmortem findings and 100 blood samples Elected randomly without considering postmortem finding). The serum from each sample was separated, properly labelled and stored at -20°C.
For the preparation of antigen, crude cyst fluid was aspirated aseptically from hydatid cysts. Blood from healthy sheep was collected in 3.8% sodium citrate solution and red blood cells were separated by centrifugation. A 2.5% red cell suspension was prepared in Phosphate Buffered Saline (PBS). The sheep erythrocytes were sensitized by Hydatid fluid antigen. Optimal dilution of antigen 1:16 was used in Phosphate Buffered Saline (p11 6.4) for sensitizing the sheep erythrocytes. All the sera were inactivated at 56°C for half an hour and serial two f1d serum dilutions were prepared by micropipettes in microtitre U plates and sensitized erythrocytes were added to the plates and incubated at room temperature in a humid chamber for 3 hours. A titre of 1:32 and above was considered as positive. In positive reactions, the cells agglutinated like a carpet at the bottom of the wells where as in negative cases the cells settled as a compact mass in the centre of the wells. By the indirect haemagglutination test Ninety-two percent sheep were found positive for hydatidosis. (Table-4.2). Out of 50 serum samples (Group A2), 46 (92%) were confirmed positive on postmortem while 3 out of 50 (6%) hydatid free samples (Group A3), gave false positive results with IHA test.
It was concluded that indirect haemagglutination test is an accurate, reliable and sensitive test (92%) for the diagnosis of hydatidosis in sheep.
The blood cell counts (TLC, DLC) and blood chemistry (Total protein, A/G ratio) of the samples under investigation were also carried out. From the results it was evident that the hydatid cysts did not affect the blood values of the host significantly. However, only 28% of hydatid positive animals showed eosinophilia ranging from 7 to 23% which was non pathognomonic.
It was thus inferred that blood cell counts and blood c1vmistry of the hydatid cyst patients was of no diagnostic value.
Availability: Items available for loan: UVAS Library [Call number: 0359,T] (1).
2.
The Incidence And Pathology Of Neoplasms Of Buffaloes In Sargodha District
by Khalid Bashir | Dr. Shakeel Akhtar Khan | Dr. Muhammad | Dr. Shahzada Khurram Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: One hundred and forty tissue specimens (suspected neoplasms) were obtained from buffaloes in District Sargodha. These specimens were examined macro and microscopically. It was found that 37 neoplasms were benign and 63 neoplasms were malignant. The benign connective tissue tumours accounted for fibroma (12), melanoma (5). Benign epithelial tumour included papillorna (20). The malignant connective tissue tumour was sarcoma lympho(20) and the malignant epithelial tumour included ocular squamous cell carcinoma (35), horn cancer (6) and Adeno-carcinoma (2).
Availability: Items available for loan: UVAS Library [Call number: 0361,T] (1).
3.
Serosurveilance Of A Disease Complex In Commercial Broilers
by Hassan Rizvi, Syed | Dr. Muhammad Naeem | Dr. Muhammad | Dr. Muhammad Sheikh Amin | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: In this study 400 samples of blood obtained from different farms situated around Jaranwala, an important broiler raising area of Punjab, were examined serologically. The Newcastle disease virus titer in most cases were low and non protective against exposure to field strain. The birds had antibodies against pathogens such as Infectious bronchitis virus and Infectious bursal disease virus to which they had not received vaccine indicating the presence of field strain of these viruses in the area. Some of the birds at these farms had antibodies against Hycoplasma galliseplicum, Mycoplasnia svnoviae and Salmonella gallinarum. Poor hygienic conditions coupled with poor ventilation may lead to the rapid dissemination of these pathogens within a community of birds and cause severe economic problems.
Availability: Items available for loan: UVAS Library [Call number: 0382,T] (1).
4.
Evaluation Of Uretero Jejunocolostomy For Urinary Diversion In The Dog
by Aneeta Hussain | Dr. Mazhar Iqbal | Dr. Muhammad | Dr. Muhammad Arif Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: Urinary diversion is the procedure adopted for urine expulsion in the most natural way, in the absence of the urinary bladder. In the beginning of the twentieth century many surgeons of human side as well as veterinarians used intestines for performing urinary diversion. They adopted many methods for this purpose but only a few were found to be successful. These procedures were used to relieve the animal from malignant bladder carcinomas, severe damage of bladder and urethra during accidents, and extrophy of urinary bladder etc. These diseases mostly cause urinary discomfort, incontinence of urine and painless heamaturia and cystectomy becomes a necessity.
During this study fifteen healthy dogs of either sex were used. Laparotormy was performed under aseptic precautions. The small intestine was exteriorized through. the incision and the terminal portion of jejunum was identified. A 6-8 cm portion of the jejunurn was selected and milked to clear it of from the feacal materials. This portion was isolated from rest of the intestinal tract with the Doyen's intestinal clamps and was cut off with its mesenteric blood supply remaining intact. The continuity of the small bowel was restored by end to end anastomosis. The vent in mesentery was closed by simple continuous sutures. The isolated Jejunal segment was wrapped in a piece of gauze soaked in normal saline.
The urinary bladder was emptied in females by gentle digital pressure and in males by mean of a disposable syringe. The ureters were amputated obliquely down to the trigone area and bladder was excised. Afterwards one end of the Jejunal segment was closed by lambert sutures. A small hole was created on the right side at the upper third portion which cut through the first three layers. From this hole a 2 cm long submucosal tunnel was created going towards the open end of the segment. The cut end of the ureter was sutured to the mucosa of the Jejunal segment using 3/o prolyene simple interrupted sutures. On the left side of the segment the other ureter was implanted similarly.
A longitudinal incision equal to the diameter of jejunal segment was made at the antimesenteric border of the distal portion of colon. The open end of jejunal segment was implanted here using 3/0 chromic catgut via side to end anastomosis with simple I nierrupted crushing sutures, thus creating an intestinal bladder. Finally the abdomen was closed in routine manner. It was concluded on the basis of this study that:
1. Viable segment of small bowel with blood and nerve supply intact can be used as a bladder.
2. Voiding occurs in the most natural possible way without any need of an external reservoir.
3. There was no ascending infection, so kidneys remained normal.
4. Use of antibiotics for rest of the life was not needed.
The successful attempt of making a new bladder strengthened the belief in cases where there was no other way of saving the animal's life except surgical intervention. So the veterinarian should involve himself with the core of his heart in the operative procedure with the aim of achieving good results. Whether he loses or gains, he must have a satisfaction of being loyal to his profession.
Availability: Items available for loan: UVAS Library [Call number: 0423,T] (1).
5.
Induction Of Ovulatory Oestrus In Non Cycling Buffalo Heifers During Summer
by Iqbal Rahim | Dr. Kazim Raza Chohan | Dr. Muhammad | Dr. Muhammad Aleem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: Late maturity in buffalo is one of the major problem which limits its overall productive and reproductive performance. Summer anoestrus plays role to delay the onset of puberty. The present study was therefore conducted to induce oestrus in non-cycling buffalo heifers during summer. Eighteen healthy non-cycling buffalo heifers with 431.38±41.5 kg body weight and 1190.44±170.92 days of age were utilized. These animals were randomly divided into three equal groups viz; group A, B and C; each comprising of 6 animals. Heifers in group A were inserted an ear implant containing (3 mg norgestomet) for 9 days and an injection of oestradiol valerate at implant insertion. At implant withdrawal 700 IU of PMSG was administered intramuscularly. Later these animals were inseminated twice at 12 and 20 hours after the onset of oestrus. The heifers in group B were given 2 injections of 500 pg cloprotenol (2 cc estrumate) at 11 days interval and were given fixed time insemination at 72 and 96 hours after second injection. The heifers in group C received no treatment and served as controls. The animals in oestrus from group C were inseminated at 12 and 20 hours after the onset of oestrus. The rectal palpation was performed for ovarian structures twice in a week for two consecutive weeks and at oestrus for follicular development and on day 8 and 10 after oestrus for corpus luteum. Pregnancy was diagnosed on day 60 post insemination. Blood samples were collected on day 8 and 10 after oestrus for progesterone estimation. Progesterone concentration was estimated by enzyme immuno assay technique. All the heifers in group A exhibited oestrus within 49.83 ± 12.65 hours; after PMSG injection. No animal from group B exhibited oestrus whereas only one heifers from control group C exhibited oestrus. The duration of oestrus in buffalo heifers treated with Norgestomet ear implant was 18.00 ± 0.30 hours. The only heifer from group C remained in oestrus for 20 hours. Only one animal (16.66%) conceived at induced oestrus in group A. None of the animal from group B and C became pregnant. Progesterone levels were found to be indicative of ovarian activity in experimental animals. Plasma progesterone concentration of 0.61 ± 0.16 ng/ml was observed on day 8 and 10 in animals showing inovulatory oestrus from group A whereas progesterone values of 5.2 and 0.7 ng/ml were observed in ovulating animals, exhibiting the presence of active corpus luteum. Low progesterone levels of 0.62 ± 0.17 ng/ml were observed in heifers from group B showing the non functional status of the ovarian. Plasma progesterone levels of 5.2 and 7.5 ng/ml was observed on day 8 and 10 after insemination in the only heifers from the control group C. The remaining 5 animals were having smooth ovaries with basal levels of plasma progesterone (0.53_+ 0.9ng/ml). In conculsion Norgestome treatment was found to be effective for induction of oestrus in buffalo heifers during summer. However studies whoud be undertaken to determine the reasons of lower conception rate. Prostaglandin (PGF2a) treatment was found ineffective in noncycling true anoestrus buffalo heifers.
Availability: Items available for loan: UVAS Library [Call number: 0424,T] (1).
6.
Comparative Evaluation Of Uretero Jejunostomy & Uretero Gastrostomy In The Dog
by Nabila Yousaf Ch | Dr.Mazhar Iqbal | Dr.Muhammad | Dr.Muhammad Younis | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: Cases of rupture of urinary bladder, cystectomy for carcinoma of the bladder or urethra and extrophy of urinary bladder are all important conditions in which urinary diversions is recommended. Many surgeons in madical field as well as Veterinaians have had tried and reported various corrective surgical procedures. In the past several attempts have been made in which the bladder was excised and ureters were implanted in colon (large intestine). But satisfactory results were not obtained because the bacterial count in the colon is much higher which resulted in ascending infection towards kidneys and caused Hydro-nephritis, pylonephritis or gastro-intestinal disturbances (1979, Boree).
Beside these complications, the advantage of this surgery was that the animal would be cured from all those diseases of urinary bladder or urethra which do not respond to other form of therapy. However, the aforementioned problems of pyelonephritis etc. and the life long cover of antibiotics to combat them. Thus compels the clinician to think of some better procedures, a new project comprising ureterojejunostomy and ureterogastrostomy was designed.
Eighteen healthy dogs of both sexes were used for this experimental implantation of ureters in the stomach (pyloric antrum) into the mid and distal jejunum. The urinary bladder was emptied by gentle digital pressure and excised completely after amputating the ureters obliquely down to the trigone area. The cut ends of the ureters were wrapped in sponge moistened with saline. An entrotomy incision about 5 ems long was given on the selected segment of mid or distal jejunum 10 ems cranial to this incision a small nick in between the mesenteric and antimesenteric border was made going through the first three layers. Through the nick 1.5 cm submucosal tunnel was created going towards the entrotomy incision. The ureter was pulled into the lumen of the intestine through this tunnel. The cut end of the ureter was spatulated and sutured to the intestinal mucosa using 3/0 prolyene simple interrupted sutures. The other ureter was similarly implanted on the other side of the jejunum. With the help of a probe the patency of ureter was checked and entretomy incision was closed using 2/0 chromic catgut simple interrupted crushing sutures. Finally the abdomen was closed in routine manner.
In the dogs of group No.3 the ureters were implanted in the least vescular part of the pyloric antrum through gastrotomy incision using the same procedure explained above.
It was concluded on the basis of this study that:
1) Pressure within the jejunum and stomach interfered with drainage and forced air, food particles and organisms up to the renal pelvis.
2) As the stomach and jejunum were acting as reservoir and the urine was in contact with the wall for a longer period, reabsorption of urinary solutes occured and resulted in azotemia.
3) The typical changes were hyperchloraemic acidosis with potassium depletion and this occured in every patient with uretro-jej unostomy and uretero-gastrostomy diversion.
4) When severe the patient developed loss of appetite, weakeness, thirst, listless, coma and died.
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7.
Effects Of Formalinized And Oil Based Hydropericardium Syndrome Vaccines On Haematology & Immunocompetent
by Zafar Abbas | Dr.Javed Rashid | Dr.Muhammad | Dr.Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1998Dissertation note: This study was designed to find out the effects on haematology (Hb, TLC and DLC), immune response (against NDV vaccine) and on organ body weight indices (liver, spleen, bursa of Fabricius and thymus) in broiler chickens when given two different HPS vaccines (Formalinized and oil-based HPS vaccines). Morbid livers of chickens affected with naturally occurred hydropericardium syndrome (HPS) were collected from field outbreaks to prepare the HPS vaccines and inoculum. Sixty broiler chicks were divided into three groups (A, B and C), comprising of 20 chicks in each on 10th day of experiment. At 14th day of age, chickens of groups A and B were given f-HPS and o-HPS vaccines, while group C was kept as unvaccinated control. On 28th day of age chickens of groups A, B and C were challenged with HPS inoculum. To study the effects of f-HPS and o-HPS vaccines, blood samples were collected on 12th (pre-vaccination), 26th (postvaccination) and 35th (post-challenge) day of experiment.
Haematological study revealed that f-HPS and o-HPS vaccines had no significant effects on haemoglobin concentration in groups A and B, but its concentration decreased significantly on 35th day in control group-C. Whereas TLC values increased significantly on 26th and 35th day in groups A and B, but decreased significantly on 35th day in group-C. DLC value revealed that there was relative lymphocytosis and heterpenia on 26th and 35th day in groups A and B, whereas eosinophilia on 35th day. While in control group-C there was lymphopenia and eosinopenia along with hetrophHia on 35th day. Antibody titre against Newcastle disease virus vaccine indicated that there was no significant difference in'GMHI titre values among the groups A, B and C. Results of organ body weight indices indicated that in control group-C (unvaccinated) indices of liver, spleen and bursa of Fabricius were significantly higher than those of groups A and B, whereas no effect was observed on thymic body weight index among groups A, B and C.
The results of this study suggested that f-HPS and o-HPS vaccines had no significant effect on Hb concentration, TLC and DLC values. No significant immuno-modulatory effect was noted on antibody titre against NDV vaccine of these HPS vaccines, whereas significant effect was noted on organ body weight indices of livers, spleen and bursa of Fabricius in group-C as compared to vaccinated groups.
Availability: Items available for loan: UVAS Library [Call number: 0582,T] (1).
8.
Effect Of Feeding Of Canola Meal On The Performance Of Layers
by Imran Hassan | Dr.Javed Ahmad Qureshi | Dr.Muhammad | Prof.Dr.Nisar Ahmad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2000Dissertation note: Canola a genetically improved variety of rapeseed after extraction of oil, as a meal, is the best supplement protein source among the vegetable protein sources which can be incorporated in different classes of poultry rations.
The present experiment was conducted to study the long term effect of canola meal feeding on the performance of 216 layer chicks from 19th 29th weeks. The birds were divided into six main groups, having 36 layers each and further sub-divided into 3 replicates of 12 layers each. Each replicate was treated as an experimental unit. All the managemental programs like, feeding, watering, vaccination, lighting and temperature were provided according to the recommended requirements for Babcock B300 layers.
Six experimental rations, A, B, C, D, E and F were prepared containing 0, 5, 7.5, 10, 12.5 and 15% canola meal in the layer mash, while with the same designation of rations, 0, 9, 12, 15, 18 and 21% canola meal containing starter and grower rations were fed during the previous phase of the project (Phase-I). The rations were formulated and provided according to the Babcock B300 strain requirement.
In this project, effect of feeding different levels of canola meal was checked in lieu of soybean meal on feed consumption, feed efficiency (Kilogram of feed per dozen eggs) egg production, weight of twelve eggs, egg shell thickness, chemical composition of eggs, organoleptic evaluation and economics.
Feed consumption, per dozen egg weight and egg production were significantly lower (P<O.01) with rations containing different canola meal levels as compared to soybean meal diet. While best feed conversion ratio (kilograms of feed per dozen eggs) was observed with canola meal containing ration that is significantly lower (P<O.01) as compared to soybean meal containing diet. Egg shell thickness was also significantly higher (P <0.01) with rations containing canola meal as compared to soybean meal.
Chemical composition and organoleptic evaluation of the eggs from the hens fed on soybean meal or canola meal containing ration did not show any pronounced effect. While the economics of the experimental rations indicated that rations contain canola meal were cheaper as compared to soybean meal containing rations. The overall picture of the whole experiment revealed that canola meal can replace completely soybean meal in laying phase. But it has little problem of poor feed intake which was may be due to picking problem of the birds due to smaller particle size of canola meal.
RECOMMENDATIONS
Based on the present study following are the recommendations:
1. With the energy compensation of the layer ration canola meal can be incorporated upto 15% dietary level.
2. A wide range of glucosinolates is present in canola quality of rapeseed i.e. 1-3O.smol/gm DM meal. It is very critical for formulation.
3. Canola meal should be incorporated in layer mash ration after pelleting in order to avoid the low feed intake problem.
CONCLUSIONS
Results of the present study reveals that canola is one of the best vegetable protein sources that can replace completely soybean meal in the layer diets with respect to the performance of the birds upto 30th week of age.
Availability: Items available for loan: UVAS Library [Call number: 0639,T] (1).
9.
Cholecystoduodenostomy As A Surgical Attempt To Establish Biliary Redirection In Dogs
by Hamid Akbar | Mr. Muhammad Arif Khan | Dr. Muhammad | Dr. Muhammad Athar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2002Dissertation note: Liver is the largest paranchmatous organ of body. Gall bladder is embedded in lobes of liver and it acts as a store house of bile. Liver pours its secretion into gall bladder which temporarily stores it and transports it to duodenum by bile duct which is a small pathway.
Sometimes this pathway (bile duct) is disturbed due to any of multiple reasons described earlier and bile is not transported. Hence there is an immediate need to develop a new connection to restore flow of bile. Cholecystoduodenostomy is a good technique in such cases to restore bile flow. In this project twelve healthy dogs were selected and divided into 3 groups namely, A, B and C. Group A consisted of 8 dogs in which cholecystoduodenostomy was performed. Group B consisted of 2 dogs, in which only laparotomy was experienced, whereas group C also consisted of 2 dogs acted as control.
Liver function tests were carried out in all the dogs before and after surgery. The enzyme values were increased when tested at 8 weeks post-operatively. The dogs were also monitored for various parameters to check the efficacy of technique.
All the dogs were euthanized at 12th week post-operatively to chck healing at surgical site. The analysis of results has clearly indicated that cholecystoduodenostomy is a safe and sound method in obstructive bile duct problems, and this technique can be used without putting the patient in dilemma.
Availability: Items available for loan: UVAS Library [Call number: 0745,T] (1).
10.
Effect Of Diclofenac Sodium On Experimentally Induced Aseptic Arthritis In Equines
by Dr . Aqeel Javeed | Prof.Dr.Muhammad Ashraf | Dr . Muhammad | Dr . Muhammad Ovais Omer.
Material type: ; Format:
print
Publisher: 2002Dissertation note: Aseptic arthritis of the knee joint was induced in 12 healthy donkeys, weighing 60 to 85kg by intra-articular injection ot O.3m1 of turpentine oil. The animals were randomly divided into 2 groups A and B of 6 animals each. Group A served as a control whereas in Group B anti-arthritic drug, diclofenac sodium (Diclocare 5%) was administered intramuscularly at the dose rate of 2.5mg/kg body weight daily for 5-6 days starting one day after the induction of arthritis. In group B, the joint circumference became normal on 6th day after induction of arthritis while in group A (control), the joint circumference took 16 to 20 days to become normal. According to this trial, diclofenac sodium provided an early return to normal stance and weight bearing in treated group B, taking longer time in control group A. No significant change in blood Haemoglobin, Total erythrocyte count, Total leukocyte count, Erythrocyte sedimentation rate and Packed cell volume was observed in control and treated animals. It is concluded that diclolènac sodium is an effective treatment for arthritis in donkeys.
Availability: Items available for loan: UVAS Library [Call number: 0780,T] (1).
11.
Effect Of Various Extenders On Semen Characteristics Of Beetal Buck (Capra Hircus)
by Aoun Raza | Prof. Dr. Muhammad Aleem Bhatti | Dr.Muhammad | Dr.Muhammad Ashfaq Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2003Dissertation note: The artificial use of germ cells of genetcally superior bucks can enhance
the growth potential of goat population to meet the shortage of mutton meat in Pakistan for daily slaughtering, sacrificial events, skin, hair and goat milk too.
Beneficial use of superior germ cells can be made only when there is extension of life of germ cells for long periods, maintenance of motility of spermatozoa and increase in number of doses of ejaculate, for which an extender of choice has to be developed. Semen characteristics of forty ejaculates of bucks were evaluated. Pooled samples of ejaculates having motility estimates of at least 60% were used for evaluation. After washing of seminal plasma with physiological normal saline (20% ringer solution) and centrifugation at l000xg for 10 minutes to remove the sperm toxic factor Lecithinase-A. Pooled semen samples were extended in Tris yolk fructose citric acid (TYFCA), milk yolk (MY) and egg yolk citrate (EYC) extenders. Samples were extended using one
step extension at a ratio of 1:60 in such a way that each milliliter of semen contained 30x106 progressively motile spermatozoa. Finally extended semen samples were placed at 5°C and 37°C for evaluation of motility percentage after every 24 hours interval and livability (hours) and absolute index of livability o spermatozoa, respectively. Mean±S.E. values of ejaculates of bucks for volume, pH, mass motility, individual motility percentage, sperm cell concentration, live and dead percentage, sperm abnormalities was recorded and post-extension motility percentage at 5oC, livability (hours) and absolute index of livability of of' spermatozoa of pooled semen at 37°C was recorded. Significant differences were observed (P<0.01) in post extension motility percentage at 5°C, at all intervals except at 120 hours interval where deference vas non-significant (P>0.05) between milk yolk and egg yolk citrate extenders. Post extension motility percentage at 5°C was highest in TYFCA than MY and EYC e\tenders. Post extension livability (hours) at 37°C was significantly different among three extenders at a level of probability (P<0.05) but non-significantly different among three extenders at a level of' probability (P>0.01). Absolute index of' livability at 37°C shows significant differences (P<0.01) for all extenders under statistical analysis. Livability (hours) and absolute index of' livability was higher in TYFCA than MY and EYC extenders. Based on these results and effect of extenders on semen characteristics of Beetal Buck, Tris- Yolk-Fructose-Citric Acid was developed as an extender of choice for short term preservation of' semen of' Beetal Buck.
Availability: Items available for loan: UVAS Library [Call number: 0798,T] (1).
12.
Efficacy Of A Newly Developed Herbal Diuretic Mixture In Broiler Chickens
by Mahreen Zahara | Prof.Dr.Muhammad Ashraf | Dr.Muhammad | Dr.Muhammad Ovais Omer.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2002Dissertation note: A number of diuretic drugs are presently used in poultry under the name of "flushers". These drugs play an important role in poultry disorders especially in cases of ascties, liver disfunctions, nephritis, Urates deposition, restoring normal functions of kidneys by eliminating toxic substances and extra fluid (through urinary system) and in oedematous conditions. The present study was under taken to evaluate the efficacy of a new locally prepared herbal diuretic mixture containing Cuminum Cyminum, Robonate of Poast, Sneeze wort and Salt petre and was compared with uronex in broiler chicken.
For this purpose, one hundred a day-old boiler chicks were reared under standard conditions. At the age of 28 day they were randomly divided into groups A, B, C, D and E. The birds of group A, B and C were given herbal diuretic mixture at different dilutions 1%, 2% and 3% in drinking water. The birds of group D were given uronex in drinking water and birds of group E were given with normal water, being control group. Diuretic efficiency of this herbal mixture was evaluated by measuring total serum protein, electrolytes (sodium and potassium) creatinine and uric acid contents before and after drug administration in broiler chicks.
Non significant (P>O.05) changes were observed in the serum protein and serum creatinine levels. The values of serum sodium, potassium and uric acid were significantly (P< 0.05) decreased in treated birds after medication as compared to control birds.
From these observations it is concluded that the newly prepared herbal diuretic mixture was effective and can be successfully used for the elimination of toxic and oedematous fluids in broiler chicks.
Availability: Items available for loan: UVAS Library [Call number: 0813,T] (1).
13.
Effects Of Excessive Sodijm Salts In Feed Of Broiler Birds
by Farrukh Ali Khan | Dr. Shakil Akhtar Khan | Dr.Muhammad | Dr.Zafar Iqbal Chaudhry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2003Dissertation note: This study was conducted on broiler birds for 6 weeks to investigate the effect of excessive sodium salt i.e. sodium chloride and sodium bicarbonate on immune status of birds, against NDV by haemagglutination inhibition test. Presence of edematous lesion on postmortem examination, estimation of serum sodium concentrations by spectrophotoinetry and calculation of FCR and weight gain was also performed. A total of 100 (one day old) broiler chicks were purchased and were divided in 4 groups A, B, C and D. The birds of group A were fed on 0.36% NaCI in feed, group B was fed on 0.36% NaHCO3 in feed and the birds of group C were fed on feed having 0.18% NaC1 and 0.18% NaHCO3. The group D was control group fed on routine feed having 0.18% total sodium salts. They were maintained under similar hygienic managemental conditions and were provided feed and water ad libitum
The results showed that the birds of group A had better feed conversion ratio as compared to the birds of group B, C and D. Whereas birds of group D had poor FCR as compared to the birds of group A, B and C. The results showed that birds of group A had better weight gain as compared to the birds of group B, C and D. Whereas birds of group D had poor weight gaul as compared to the birds of group A, B and C. On analysis of serum sodium concentration, the birds of group A had maximum sodium concentration and birds of group D had lowest serum sodium concentration. Statistical analysis showed a significant difference in the serum sodium levels of all groups except within group B and C. The highest GMHI titer against ND virus was observed in sera of birds of group D and the lowest ill the sera of birds from group A. No edematous lesions were observed in birds of any group.
Availability: Items available for loan: UVAS Library [Call number: 0839,T] (1).
14.
Effect Of Vitamine (Alpha-Tocopherol) Supplementation On Total Lipid Profile In Rabbits
by Aman Ullah | Prof.Dr.Muhammad Ashraf | Dr. Muhammad | Mr. Shahid Abbas | Faculty of Biosciences.
Material type: ; Format:
print
Publisher: 2005Dissertation note: An experimental trial of forty five days duration was conducted in July and August, 2004 at Physiology Department, Faculty of Bio-Sciences, University of Veterinary & Animal Sciences, Lahore. The study was conducted to see the effect of vitamin E (alpha tocopherol) on total lipid profile in rabbits. Thirty two, 2 months old white rabbits were purchased, weighed and randomly divided into four groups, comprising of eight rabbits each i.e. group A, B, C and D. The rabbits of group A were fed on normal diet and acted as control animasl. The rabbits of group B were also fed on normal diet along with replacement of 2% cholesterol. The objective to add cholesterol is to induced hypercholesterolemia artificially from the beginning of the experiment. They were orally administered cholesterol powder through disposable syringes from start to end of experiment. During the experiment these rabbits were not treated with vitamin E (alpha tocopherol). The rabbits of group C were also fed on normal diet alongwith replacement of 2% cholesterol for first fifteen days of experiment to induced hypercholesterolemia. After the induction of hypercholesterolemia these rabbits were treated with vitamin E at a dose rate of 50 mg/kg body weight. The rabbits of group D were also fed on normal diet alongwith replacement of 2% cholesterol for first fifteen days of experiment to induced hypercholesterolemia. After the induction of hypercholesterolemia these rabbits were treated with vitamin E at a dose rate of 75 mg/kg body weight. The feed for rabbits were prepared and were given with green fodder twice a day. The rabbits were reared in room carpeted with dry forage and dry soil, from the start to the end of the experiment. Standard conditions of feeding, water and temperature were maintained. The rabbits were kept in light for 24 hours throughout the experimental period. Biochemical analysis i.e. serum cholesterol, serum low density lipoprotein (LDL), serum triglycerides (TG) and serum high density lipoprotein (HDL) carried out. It was concluded that vitamin E has significant effect on total lipid profile of hypercholesterolemic rabbits. Vitamin E as an antioxidant can reduce the incidents of atherosclerosis in hypercholeterolemic rabbits and ultimately reducing the risk of heart disease in rabbits as a human model.
Availability: Items available for loan: UVAS Library [Call number: 0888,T] (1).
15.
Seroprevalence Of Ftoxoplasma Gondii Infection In Sheep And Goats
by Sher Hayat Khan | Prf.Dr. Azhar Maqbool | Dr. Muhammad | Prof. Dr. Haji Ahmad Hashmi | Faculty of Veterinary Sciences.
Material type: ; Format:
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not fiction
Publisher: 2005Dissertation note: Keeping in view the zoonotic importance of Toxoplasmosis the present study was carried out to determine the seroprevalence of Toxoplasma gondii infection in sheep and goats using commercially available kit "Toxopalasmosis Latex" based on the principal of Latex agglutination. . For this purpose 100 blood samples (50 sheep & 50 goats) were collected randomly from slaughter house of Lahore and local sheep & goat forms, the serum was separated and analyzed for anti Toxoplasma antibodies. The animals were divided in three groups, Group I included animals below one year, Group II 1-4 years and Group III included animals aged 4 years and above. The overall 8% and 6% seroprevalence were recorded in sheep and goats respectively. Age titer relationship in sheep showed that Group III had (12.5%), Group 11(7.6 %) and Group I had no positive cases in sheep examined. In goats Group III had (10 %), Group II (5.7%) and no seropositive cases in Group I could be recorded. These results indicate that the infection was higher in older animal than young ones, Breed wise seroprevalence indicated that, Kchhi sheep 14.28%, Desi (non descript) 8.33% and no seropositive case in Lohi and Thali breeds was recorded. In goats Teddy breed had 11.11%, Desi (non descript) 5.55% while no seropositive case in Beetal goats could be recorded.
From the data analyzed it is concluded that although the seroprevalence of T gondii infection in sheep and goats is relatively low in this area as compared to the other parts of the world. Yet adequate management might be useful and essential to further minimize the infection in sheep and goats herds. Consequently, preventive measures could further reduce the exposure of toxoplasmosis in the human population.
Availability: Items available for loan: UVAS Library [Call number: 0899,T] (1).
16.
Determination Of Apparent Metabolizable Energy Of Indigenous Vegetable Protein Sources In Poultry
by Hammad Ali | Prof.Dr.Talat Naseer Pasha | Dr. Muhammad | Mr. Muhammad Kamran.
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Publisher: 2006Dissertation note: An experiment was conducted to determine the apparent metabolizable energy (AME) and true metabolizable energy (TME) of indigenous vegetable protein sources in cockerels by force feeding method. Six indigenous vegetable protein sources viz. soybean meal, canola meal, rapeseed meal, guar meal, sunflower meal and linseed meal were used. Thirty five 18 weeks old broiler breeder cockerels were used in such a way that five birds were force fed 25 gm of each feedstuff for estimation of AME and TME. Five birds were kept fasted to serve as negative control for determination of metabolic faecal and urinary endogenous energy excretions. The pre-feeding fasting period was 24 hours and excreta was collected over a period of 24 hours. The gross energy of test ingredients and the excreta was measured by using adiabatic oxygen bomb calorimeter. Proximate analysis for moisture, crude protein, ether extract, crude fiber, ash and nitrogen free extract were carried out. All had almost similar dry matter and ether extract values. Sunflower meal and canola meal had less CP% because of presence of sulphur containing amino acids. The observed AME values for soybean meal, canola meal, rapeseed meal, guar meal, sunflower meal and linseed meal were 2556, 1920, 1887, 1847, 1629 and 2061 Kcal/Kg, respectively. Soybean meal had the highest value 2556 Kcal/Kg and sunflower meal had the lowest value 1629 Kcal/Kg. The observed TME values for soybean meal, canola meal, rapeseed meal, guar meal, sunflower meal and linseed meal were 2694, 2053, 2022, 1992, 1764 and 2198 Kcal/Kg, respectively. Soybean meal had the highest value 2694 Kcal/Kg and sunflower meal had the lowest value 1764 Kcal/Kg. Endogenous energy ranged from 2.93 Kcal/gm to 2.95 Kcal/gm with the average of 2.94 Kcal/gm. It was also found that crude fiber has indirect relationship with both AME and TME. While, it has also come to notice that ether extract has direct relationship with gross energy of the feedstuff.
Availability: Items available for loan: UVAS Library [Call number: 0936,T] (1).
17.
Effect Of Detomidine And Chloramphenicol On Propofol-Induced Ana Esthesia
by Salamat Ali | Prof.Dr.Muhammad Ashraf | Dr. Muhammad | Dr. Muhammad Ovais Omer.
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Publisher: 2007Dissertation note: In clinical practice anaesthetics are of great importance as for as surgeries are concerned whether in human beings or animals. This project was designed to evaluate the effect of Chioramphenicol and Detomidine on Propofol-induced anaesthesia in mice and dogs. The study was conducted in two separate experiments. In first experiment, sixty mice were divided into six groups; group A, group B, group C, group D, group E and group F. Each group comprised of ten mice. Group A was treated with Propofol (100 mg/kg). Chioramphenicol pretreatment (100, 150, 200mg/kg), 15 minutes prior to administration of Propofol (100 mg/kg) were used in Group B, C, D. Group F was treated with normal saline 0.3 ml only. Both the drugs were given as intraperitoneal injection. In second experiment, twelve dogs were divided into three groups i.e. group A, B and C. Each group comprised of four dogs. Group A was treated with Propofol (6 mg/kg, I/V) as a single bolus, while group B was given Detomidine hydrochloride (40 pg/kg body weight) intramuscularly as a premedication 15 mm before Propofol intravenously (6 mg/kg) while group C was treated with 3 mg/kg Propofol along with 40 pg/kg of Detornidine as premedication. After a wash out period of two weeks the dogs of group A were treated with Detomidine hydrochloride (40 jig/kg body weight) intramuscularly as a premedication 15 mm. before the Propofol intravenously (6mg/kg body weight) while, the dogs of group B were treated with Propofol 6 mg/kg body weight intravenously as a single bolus and group C was repeated with the 3 mg/kg Propofol along with 40 pg/kg of Detomidine as premedication. Anaesthetic parameters were noted. Serum level of Calcium, Chloride, Potassium and Sodium fell and were normal after recovery.
The results indicated that induction time remained same and was smooth with these premedication while duration of anaesthesia increased to threefold compare to control groups and uncomplicated recovery was recorded.
The collected results from this study conclude that this premedication gives better results and should be used in clinical practice.
Availability: Items available for loan: UVAS Library [Call number: 0976,T] (1).
18.
Morphology Of Some Bat Species Of District Bajaur Agency And Adjacent Areas
by Hamid-ullah | Dr. Muhammad Mahmood-Ul-Hassan | Dr. Muhammad | Dr. Muhammad Ali Nawaz.
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Publisher: 2009-2011Dissertation note: This one year study extending from June 2010 to May 2011 was made to collect information on various bat species inhabiting Bajur Agency (FATA) and some adjacent areas (KPK). A total of 30 bats were captured and measured for body and cranial parameters during the present study. Of these twenty specimens were collected from Bajaur Agency and ten from Dir (Lower). All the potential roosting sites were thoroughly searched and their GPS locations were recorded. Bat samples were collected through mist nets and hand nets. Each captured specimen was tagged in the field indicating a specific field number, date and time of capture, exact locality, age, sex and the name of town or village of Bajaur Agency. They were preserved in 95% Alcohol before weighed, measured and autopsied. The standard morphometric, cranial and bacular measurements of each specimen were recorded and compared with that given in the literature.
Twenty bats were captured from two Tehsils of district Bajaur Agency (SA1). Fourteen bats were captured from Tehsil Khar. These included Scotophillus heathii (1?) captured from Molakaly, Pipistrellus ceylonicus (1?, 1?), Pipistrellus tenuis (1?) Pipistrellus cromendra (1?), Pipistrellus javanicus (1?) and Scotophilus kuhlii (2?) captured from Tuhaidabad and Pipistrellus cromandra (1?, 5?) captured from Daray. Six bats were recorded from Teshil Salarzo. These included Pipistrellus pipistrellus (2?), Pipistrellus tenius (1?, 1?) and Pipistrellus javanicus (1?) captured from Malkana and one Rhinolopus hipposiderous (?) captured from Tattay. Ten bats were captured from Dir (Lower). These included Megaderma lyra (1?,4?) Rhinolopus ferrumequinum (1?) and Rhinolopus hipposiderous (4?).
Availability: Items available for loan: UVAS Library [Call number: 1363,T] (1).
19.
Prasitic Contamination Of Vegetables Eaten Raw In Lahore
by Shafa-ul-Haq | Prof. Dr. Azhar Maqbool | Dr. Muhammad | Dr. Nisar Ahmad.
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not fiction
Publisher: 2012Dissertation note: Fresh vegetables are important part of human diet. Eating raw vegetable is customary in many parts of the world including Pakistan, but these raw vegetables can be a major source of parasites. A parasitic survey on vegetables collected from major markets in Lahore was conducted for the discovery of human and animal parasites. Ten species of vegetables: beet (Beta vulgaris), cabbage (Brassica denceal), carrot (Davcus carota) chili (Capsicum frutecense), coriander (Corriandum sativum) cucumber (Cucumis sativa), lettuce (Lactuca sativa), Mint (Mentha viridis), radish (Raphanus sativus), tomato (Lycopersicon esculentun) were evaluated in this study. Fifty samples of each vegetable were taken, comprising a total of 500 samples for the study.
Hundred gram of each vegetable was washed with a cationic solution of Hyamin detergent containing glass particles for the elution of eggs. Concentration of eggs/cysts was achieved by centrifugal sedimentation technique at 5000 rpm for 5 minutes. Sediment was examined under microscope for the presence of parasite eggs, cysts and larvae.
Examination of vegetables revealed twelve genera of parasites. All vegetables were highly contaminated with parasites with an overall prevalence of 31.2%. Of parasites studied, Ascaris eggs found to be the highest (37.1%), followed by Hook worm(10.8%) and Trichostrongyloides sp.(8.9%), while the least common parasite was Toxoplasma gondii (1.9%).
Lettuce showed the highest contamination (48%), followed by Cabbage (44%) and Mint (podina) (42%) while chili showed the least contamination (16%).
The results would seem to indicate that one of the important routes of parasitic infection is due to consumption of raw and unwashed vegetables. So, enlightenment programs for the public on necessity of food sanitation and personal hygiene should be intensified.
Availability: Items available for loan: UVAS Library [Call number: 1419,T] (1).
20.
Dna Barcoding Of Pakistan Avian Families (Sturnidae & Columbidae)
by Emma Umar | Dr. Ali Raza Awan | Dr. Muhammad | Ms. Saeeda Kalsoom.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1515,T] (1).
21.
Higher Production Of Algnate By A Mutant Of Azotobacter Vinelandii By Fermentation
by Saria Rahim | Ms. Shagufta Saeed | Dr. Muhammad | Ms. Huma Mujahid.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1521,T] (1).
22.
The Diagnostic Value Of Tta Codon Substitution In Los Angeles Galactosemia
by Sahr Malik | Dr. Muhammad Imran | Dr. Muhammad | Miss. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1590,T] (1).
23.
Mutational Analysis Of Myelocytomatosis (Myc) Gene Isolated From Dog & Cat Tumours.
by Anum Kamal | Dr. Mhammad Wasim | Dr. Muhammad | Ms. Sehrish Firyal.
Material type: ; Format:
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Publisher: 2013Dissertation note: Now a day's pets, dogs & cats form an important part of society and their health care have gain much popularity among owners. The hazardous radiations, along with other ailments cause different cancers in them which are the most fatal diseases. This increase in cancer prevalence is also related to animals living to increasingly older ages due to better nutrition, vaccination and health care provided to them. In order to provide proper treatment and care to these animal cancer patients, it is important to gather knowledge about the genes involve in causing them. There are many genes which regulate the cell cycle progression, cell proliferation and cell differentiation. Here in this study we have tried to understand this dreadful disease at molecular level. And hoping the knowledge gain through this would help in providing better treatment in future, not only to pets but also to humans. Dogs and humans share anatomical and physiological similarities, and a large number of cancers are diagnosed and managed to some extent in dogs annually. More importantly the basic biology of cancer in dogs is analogous to human cancers. According to an estimate every fourth dog suffers with cancer.
The deregulation and mutations in proto-oncogenes are the subjects under study. One such proto-oncogene is Myc, whose translated protein act as a transcription factor and regulates the expression of various genes. Myc protein binds DNA at specific sites, i-e e-box sequence and initiates the transcription of other genes, and controls their expression. Myc is assumed to regulate 15% of all cellular genes. Elevated expression of Myc is spotted in about 70% of all cancers.
The sampling was done from pet dogs and cats, belonging to different breeds with diagnosed tumor type, from the pet centre of university of veterinary and animal sciences, Lahore and various private pet clinics. 3-5 ml blood was collected aseptically and genomic DNA was extracted from blood using inorganic extraction method & its quantity was checked by NanoDrop spectophotometer. Four primer sets were designed to amplify protein coding region of Myc gene. After amplification through PCR, DNA sequencing was done. Data interpretation was done by using several softwares like BLAST alignment tool, Chromas Lite, Mega 5.2, Phyre 2, VMD 1.9.1, & PyMOL. A polymorphic change was detected in the protein coding region of Myc gene, which causes an amino acid substitution at lys355 by Arg, thus changing the Myc protein sequence. But this change might not affect protein structure much, as in some bHLH proteins Arg355 resides normally. Some changes in the 3'UTR were also detected which might play crucial part in stabilizing the Myc protein, by altering silencer box or miRNA binding sites. Thus high level of stable Myc protein causes increase cell division leading to tumor production.
This study will make available the genetic data and contribute substantial addition in the existing information in animal genetic resources. It would also aid in future to assess the possibility whether Myc can serve as a useful marker for diagnosis and prognosis of these malignancies. The need for today is to develop valid biomarkers, which can be incorporated in ongoing in vivo and in vitro clinical mechanistic and improve the diagnosis & prognosis of this dreadful disease.
Availability: Items available for loan: UVAS Library [Call number: 1604,T] (1).
24.
Molecular Epidemiology Of Subclinical Tuberculosis In Peri-Urban Human Population Of Lahore.
by Sadeem Shahzad | Dr. Muhammad Yasir Zahoor | Dr. Muhammad | Prof. Dr. Tahir Yaqub.
Material type: ; Format:
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drama
Publisher: 2013Dissertation note: Tuberculosis (TB) is known to be a major health problem worldwide causing disease among millions of people every year. Major cause of tuberculosis in human is the infection with M.tuberculosiswhich usually causes pulmonary or lungs TB but an unknown number of patients are also infected with M.bovis which causes tuberculosis in humans as zoonotic agent along with its major hosts like cattle and deer. In developing countries where raw milk is used without pasteurisation there is a heavy risk of tuberculosis infection with M.bovis. TB infection with M.bovis mainly appears as extra pulmonary tuberculosis with and without specific symptoms of the disease.Diagnosis of subclinical asymptomatic tuberculosis and that of extra pulmonary tuberculosis is a difficult task and most of the time disease remains undiagnosed or misdiagnosed due to the unavailability of specific and sensitive diagnostic tool to diagnose the disease at early stage. Moreover prevalence of M.bovisinfection is not properly known.
This study was designed to measure the diagnostic value of Interferon gamma release assay (IGRA) for early and reliable diagnosis of subclinical extra pulmonary TB along with the molecular epidemiology of subclinical extra pulmonary TB to check the prevalence of M.bovisinfection. IGRA is a latest blood test with high specificity and sensitivity based on the principle of Interferon gamma released by effector T-Cell when exposed to M.tuberculosis antigens like ESAT-6 and CFP-10 in controlled in-vitro conditions. Eighty patients were selected for the study on the bases of the history of having day to day cattle contact along with feelings of sickness. Biopsy tissue samples of all the patients which were positive with IGRA were requested, however 24 out of 27 positive samples were collected and were first examined histologically. Twenty seven samples out of eighty were found positive with IGRA while 22 out of 24 samples were confirmed by histological examination as infected with MTB.
Both IGRA and histological examination are unable todifferentiate between the specie specific infection with M.tuberculosis orM.bovis for which differential amplification of specific fragments of bothof the species was done by running a multiplex PCR using M.tuberculosis specific 185 bp pncA product and M.bovis specific 500 bp segment. Genomic DNA was extracted from previously formalin fixed paraffin embedded (FFPE) tissues which requires pretreatment for deparaffinization. Xylene was used as deparaffinization agent. All of the twenty two samples positive with IGRA and histological study were found positive for M.tuberculosis infection and none of the sample was found positive for M.bovis infection. Results showeda close correlation among all three techniques with their specific benefits and limitations.
Study concluded that T.Spot TB (IGRA) is a potentially reliable test for the diagnosis of subclinical, extrapulmonary TB.Formalin Fixed Paraffin Embedded (FFPE) tissues may be used for TB diagnosis and other DNA based researches. Duplex PCR is a reliable technique for differential diagnosis of infection with different species of MTB complex, though none of the sample was found positive for M.boviswhich is may be due to small sample size of the study and it may further be studied in future researches. The research findings will help the clinicians to depend on IGRA testing for timely and reliable diagnosis of extrapulmonary subclinical tuberculosis and potential use of FFPE tissue samples as appropriate specimen for molecular based diagnosis of TB. Further studies are however, required to check the prevalence of M.bovis infection byincreasing sample size.
Availability: Items available for loan: UVAS Library [Call number: 1621,T] (1).
25.
Leptin Mutations In Morbidly Obese And Severely Lean Individuals From Pakistan
by Muhammad Wasim | Dr. Sehrish Firyal | Dr. Muhammad | Dr. Muhammad Imran.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1623,T] (1).
26.
Analysis Of Medulla In Human Head Halr In Different Castes
by Summaiya Aurangzeb | Dr. Wasim Shehzad | Dr. Muhammad | Dr. Muhammad Tayyab.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1633,T] (1).
27.
Assessment Of Parasitological Contamination Of Fresh Ad Dry Fruits In Lahore
by Muhammad Anis Khan | Dr. Nisar Ahmad | Dr. Muhammad | Prof. Dr. Azar Maqbool.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Fruits are important part of human diet. Eating unwashed fruits is common in
many parts of the world including Pakistan, but these can be a major source of parasites.
A parasitic survey on fruits collected from major markets in Lahore was conducted for
the discovery of human and animal parasites. Ten species of fruits: Guava, Apple,
Sapodilla, Sweetsop, Strawberry and dry fruits: Apricot, Date, Persimmon, Fig and
Raisins were evaluated in this study. Fifty samples of each fruit and a total of 500
samples were taken for the study.
Hundred gram of each fruit was washed with a cationic solution of Hyamin
detergent containing glass particles for the elution of eggs. Concentration of eggs/cysts
was achieved by centrifugal sedimentation technique at 5000 rpm for 5 minutes.
Sediment was examined under microscope for the presence of parasite eggs, cysts and
larvae.
Examination of fruits revealed nine genera of parasites. All fruits were highly
contaminated with parasites with an overall prevalence of 35.2%. Of parasites studied,
Ascaris found to be the highest (36%), followed by Trichuris (14.3%) and
Trichostrongyloides sp.(11.9%), while the least common parasite was Fasciola (2.9%).
Fruits are the source of parasitic infestation. The most contaminated fresh fruit
was strawberry (54%) followed by guava (48%). Whereas the least contaminated fresh fruits was sweetsop (22%). The most contaminated dry fruits was fig (46%) followed by
date (38%). whereas the least contaminated dry fruits was (20%).
The results indicate that one of the important routes of parasitic infection is due to
consumption of unwashed fruits.
Conclusions:
Strong efforts should be made to prevent contamination of fruits and vegetables
during production, transport, processing and handling, much improvement is still needed
in our country if hygienic production of fruits and vegetables is to be ensured. This study
provides an overview of the hazards associated with eating contaminated fruits. Parasitic
infections like Cryptosporidiosis can be acquired by people if orchards or water sources
near cow pastures become contaminated from infected cows and people consume the fruit
without proper washing. The study needs to be carried out in other areas of Pakistan and
more fresh and dry fruits should be included in the study. Information regarding the
contamination of the fruits should be published in the national newspapers because
awareness of the public is very important for the control of these contaminations.
Availability: Items available for loan: UVAS Library [Call number: 1645,T] (1).
28.
Dna Quantification Of Blood Stains Recovered From Washed Fabrics
by Zeenat Salah-ud-din | Dr. Muhammad Yasir Zahoor | Dr. Muhammad | Dr. Muhammad Wasim.
Material type: ; Format:
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; Literary form:
drama
Publisher: 2013Dissertation note: Bloodstains are extremely important forensic evidences for DNA profiling. Bloodstains
recovered at crime scene can help to identify and confirm the credentials of the victim. Criminal
can make all efforts to destroy and hide possible evidence at crime scene therefore these
bloodstains on the crime place can hardly provide any evidence against the culprit. The criminal
will try to take away or change clothes with the bloodstains of the victim. Once perpetrator has
removed the bloodstains, culprit will feel secure and satisfied. But as a suspect if clothes are
recovered with washed bloodstains and forensic evidence of DNA of the victim can be recovered
from the washed clothes and can provide strong evidence against suspect. Present study has been
planned to quantiate DNA from 10 different types of bloodstained fabrics with possible ways of
washing to hide the blood from fabrics. 100 ìl of preservative blood was used in creating blood
spot on fabric. After that dried bloodstained fabrics were hand washed with tap water and in
detergent for 5 and 10mins and air dried at room temperature (25°C- 30°C) in laboratory. DNA
was extracted through Chelex® 100 extraction procedure from these fabrics and analyzed on
Real time PCR kit i.e. Quantifiler™ Human DNA Quantification kit which is specific for Human
DNA. Research study showed there was no statistically significant difference in DNA
concentrations among different washing methods (p-value = 0.318). There was statistically
significant difference in DNA concentrations among different types of fabrics divided into two
categories (p-value = 0.001, 0.002). Research findings showed that cellulose fibers recovered
highest quantity of DNA, followed by artificial fibers and then protein fibers (cellulose
>artificial>protein fiber) despite their washing methods. Thus provides a lead in solving such
type of criminal cases.
Availability: Items available for loan: UVAS Library [Call number: 1663,T] (1).
29.
Gender Differentiation From Fingerprint Ridge Count In Pakistani Population
by Ahmed Fayyaz | Prof. Dr. Tahir Yaqub | Dr. Muhammad | Ms. Sehrish Firyal.
Material type: ; Format:
print
Publisher: 2013Dissertation note: In forensic science, fingerprinting has been used for decades as an efficient tool for identification of persons linked to an illegal activity or a crime scene. Different methods for the development and analysis of the latent fingerprints have been introduced including optical, physical and chemical methods. Each method has its own importance in the development and examination of the latent prints, which are invisible to naked eye before the application of fingerprint development methods. A lot of work has been published worldwide regarding fingerprinting. It was also reported that there is a significant difference in the ridge density of males and females. Ridge count might be helpful in the gender differentiation in Pakistani population. Patent prints of 100 males and 100 females were taken on A4 size paper or card paper using pelikan black inkpad and analysis was done with the help of 10x magnification lens. The ridges were counted diagonally within a square of 5mm x 5mm. This value depicts the number of ridges per 25 mm2. Results were analyzed by using Multivariate analysis of variance (MANOVA). The results of this study are used as a helpful tool for forensic expert and law enforcement. It reveals that females have finer epidermal ridge detail than males. The degree of ridge density is used as presumptive indicator of gender of unknown print left at a crime scene. First we qualitatively examine if prints appear coarse or fine and then by quickly quantifying ridge density or ridge count in a manner similar to method described in this study. The outcomes of this study will be helpful in exoneration of innocents in different crimes.
Availability: Items available for loan: UVAS Library [Call number: 1668,T] (1).
30.
Molecular Characterization Of Antimicrobial Resistance Genes In Salmonella Isolates From Poultry
by Saba Zeb Khan | Prof. Dr. Tahir Yaqub | Dr. Muhammad | Ms. Sehrish Firyal.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 2013Dissertation note: Salmonella is a gram negative bacteria which can cause a number of different diseases including gastroenteritis, bacteremia, and typhoid fever, with the most common being gastroenteritis, some serotypes of it are pathogenic and cause serious food poisoning in humans and major economic losses in both chicken and turkeys. The birds can be the reservoir of Salmonella species which cause food borne infections in human. Human get such infections by ingesting contaminated products. In poultry farms, Salmonella can be introduced by means of contaminated feeds, particularly those that contain animal raw materials.
Use of antibiotics in poultry has become a popular practice. Different antibiotics like tetracycline, streptomycin, trimethoprim etc. are given in poultry via water and feed for growth and protection against diseases. Extensive and uncontrolled use of antibiotics resulted in increased development of antibiotic resistant bacteria. Statistical data shows that Salmonella is resistant to many antibiotics especially tetracycline.
The goal of our study was Molecular characterization of tetracycline resistance genes in Salmonella spp. and to check the prevalence of tetracycline resistance genes in Salmonella isolates from poultry drinking water.
Total 50 water samples were collected from different poultry farms and poultry meat shops in Lahore district.Various biochemical tests were performed to confirm the isolated strains as Salmonella. Tetracycline resistance was examined against isolates. Plasmid DNA was extracted from these bacteria. Antibiotic resistant plasmid genes were amplified by PCR. After gel electrophoresis the resulting fragments were sequenced through genetic analyzer. After sequencing the sequence thus obtained was compared with the reported sequences of tet genes in Salmonella strains in NCBI.
It was found out that Salmonella isolates from the poultry drinking water are highly resistant to tetracycline, as 83% of the isolated Salmonella from poultry drinking water showed their resistance towards tetracycline.PCR amplification of tet genes indicated the presence of tetA gene in 100% of tetracycline resistant Salmonella, whereas 64% of the samples contained tetB gene. TetB gene was present only in combination with tetA gene. None of the sample contained tetC, tetD and tetGgene.
This study helped to find out the prevalence of antibiotic resistant genes in Salmonella isolated from poultry drinking water, which were potential threats to human being and this study will also help us in future to develop strategies to restrict the emergence of antibiotic resistant genes and their spread.
Availability: Items available for loan: UVAS Library [Call number: 1714,T] (1).
31.
Levels Of Maternal Antibodies Against Pasteurella Multocida In New Born Buffalo Calves
by Muhammad Bilal | Dr. Aneela Zameer Durrani | Dr. Muhammad | Dr. Muhammad Ijaz.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Buffalo is called black gold. The buffalo is raised for meat and milk purpose in Pakistan.The buffalo calves are at risk of many infectious diseases. One of the most important diseases is Hemorrhagic septicemia (HS). Hemorrhagic septicemia is caused by Pasturellamultocida important strains in Asia are B:2 and B:2.5. Buffalo calves are more suspected to the Hemorrhagic septicemia than adults one. In Pakistan the high prevalence of 49% is in the rainy season (Farooqet al. 2007)
Clinically the signs of Hemorrhagic septicemia includes profuse salivation,pyrexia, respiratory distress, swelling in throat, discharge through nostrils, protrusion of tongue, edema in the brisket area as well as in forelegs. Hemorrhagic septicemia can be diagnosed on the bases of clinical signs and laboratory conformation by Gram staining and. Serum agglutination test, Counter immune-electrophoresis and ELISA techniques are also used.Enrofloxacine was found to be effective treatment in case Hemorrhagic septicemia. Best protection measure vaccination by alum precipitated vaccine (Boudewijn et al. 2008).
During the present study blood samples were collected from the calves at different intervals of times. Serum was separated from that blood and was analyzed by using Indirect Haemagglutination(IHA) Test to observe the antibodies titer in the blood.The data was analyzed to calculate geometric mean titer (GMT) of the antibodies. Immunity status is much important in the defense of disease especially in the newly born claves which are more at risk of infections.The result of present study showed geometric mean titer 16 in colostrum of vaccinated dams and their newly born claves showed 0, 3. 4, 7 and 3 at 0 hour, 72 hour, 7, 15 and 30 days of their age. Colostrum of non-vaccinated dams showed GMT 8 and their newly born claves showed 0, 1. 3, 3 and 1 at 0 hour, 72 hour, 7, 15 and 30 days of their age.The maternal antibodies which are produce in the body of mother are transferred to their calves.The calves given birth by the vaccinated dams receive much higher level of antibodies from their mother than the calves born from non-vaccinated dams. This antibodies production in non-vaccinated dams is because of the carrier status of hemorrhagic septicemia.The non-vaccinated dams which are not expose to the any type of HS infection or not expose to the vaccines, they do not produce antibodies against Pasturellamultocidaand also not transfer to their young babies after birth.
The present study showed that claves of vaccinated dams are much protected in their early period of life against hemorrhagic septicemia. Buffalo calves need quick and intense care at early age and they need quick vaccinations.
The high maternal antibodies level against Pasturellamultocidain vaccinated dams then in non-vaccinated dams antibodies levels in newly born buffalo calves remain effective for longer period.
This study suggested for the vaccination of dams not only for the protection of themselves but also for the protection of their newly born claves at the crucial period of their young age.
Availability: Items available for loan: UVAS Library [Call number: 1807,T] (1).
32.
Prevalence And Chemotherapy Of Fasciolosis In Cattle In Nangarhar Afghanistan
by Redwanullah | Prof.Azhar maqbool | Dr.Muhammad | Dr.Muhammad Lateef.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1866,T] (1).
33.
Study Of Carrier Potential Of Newcastledisease Virus In Common Wild Bird Species
by Tahir Munir | Prof. Dr. Asim Aslam | Dr. Muhammad | Dr.Ishtiaq Ahmed.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1892,T] (1).
34.
Epidemiological Study Of Zoonotic Leishmaniasis In Humans And Dogs In District Jhelum
by Behzad kayani | Dr. Mamoona chaudhry | Dr. Muhammad | Dr. Muhammad hassan mushtaq.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2000,T] (1).
35.
In-Silico Functional Prediction Analysis Of Prion Protein Polymorphisms In Sheep Scrapie
by Mubashar ahmad | Dr. Muhammad imran | Dr. Muhammad | Dr. Wasim shehzad.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2012,T] (1).
36.
In Silico Functional Prediction Of Prion Protein Polymorphisms In Chronic Qasting Disease
by Iqra khizar | DR. Muhammad imran | Dr. Muhammad | Dr. Muhammad yasir zahoor.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2024,T] (1).
37.
Microbial Quality Of Fresh And Frozen Fish From Markets Of Lahore
by Irfan iqbal khan | Dr. Naureen naeem | Dr. Muhammad | Ms. Farasat rizwan.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2068,T] (1).
38.
Pathological Studies On Contagious Edthyma In Naturally Infected Small Ruminants
by Muhammad Usman ghani | Dr. Mati ur rehman khan | Dr. Muhammad | Prof. Dr. Asim aslam.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2070,T] (1).
39.
Prevalence And Chemotherapy Of Canine Babesiosis
by Faisal Shrif | Dr. Nisar Ahmad | Dr. MUhammad | Prof. Dr. Azhar Maqbool.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2117,T] (1).
40.
Prevalence And Chemotherapy Of Trichostrongyloids In Camels In Charsada
by Muhammad Qasim | Prof. Dr. Azhar Maqbool | Dr. Muhammad | Prof. Dr. Kamran Ashraf.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2131,T] (1).
41.
Molecular Diagnosis Of Feline Babesiosis
by Muhammad Younus Khan | Dr. Haroon Akbar | Dr. Muhammad | Dr. Muhammad Lateef.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2168,T] (1).
42.
Comparative Efficacy Of Casoni Skin Test, Indirect Haemagglutination And Double Diffusion Tests For The Detection Of Hydatidosis In Goats
by Azam Mahmood | Prof. Dr. Tufail Muhammad Khan | Dr. Manzoor Ahmad Basraa | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0149,T] (1).
43.
To Study The Effect Of Different Dietary Protein & Energy Level On The Growth Performance Of Nili-Ravi Buffalo Male
by Rehman Khan, A | Dr. Nisar ahmed mian | Dr. Jaweed ahmad qureshi | Dr. Muhammad.
Material type: ; Format:
print
Publisher: 1993Dissertation note: The buffalo is an important animal in the agriculture economy of our country and the most useful animal for many millions of poor people, especially those living in Asia.
So far, no definite studies with regard to the nutritional requirements of the buffalo at various stages of growth and production (meat and milk) have been conducted. The feeding of buffalo in the sub-continent is based on various cereals and cash crops and are generally subjected to various nutritional imbalances, as a result, the genetic potential with regard to growth and production (meat and milk) of buffalo is not fully exploited.
Thus there is a desperate need for determining the specific feeding requirements of the indigenous dairy buffalo. For the purpose digestibility and metabolic studies for various nutritional needs for growth, fattening and production are to be conducted. Thus the present study was designed to study the effect of different plans of nutrition on growth, efficiency of feed utilization and nutrients digestibility in Nili-Ravi buffalo male calves.
Twenty-seven buffalo male calves of 9-12 months of age were randomly divided into nine groups. Three different levels of protein and metabolizable energy (80, 100 and 120% of NRC, 1976 Standards for beef cattle) were incorporated in the nine experimental rations.
After the trial of 140 days, the buffalo male calves gained weight 102.67, 110.64, 90.33, 36.00, 73.33, 127,34, 98.67, 82.34 and 110.34 Kgs. in groups 1-9, respectively. Maximum growth was recorded in group 6 [CP 100% and ME 120% of NRC (1976) Standards for beef cattle). Weight gain was non-significant due to varying levels of protein in the rations while weight gain was significant (P<0.05) due to varying levels of energy in the rations. Maximum growth rate of 909 gms. per day in group 6 (CP 100% and ME 120%) was recorded. The regression equation for weight gain with the relation to protein and energy intake was as follows: Weight gain (WG) = -297.28 + .425 (Pr) ± 63.67 (En)
The feed consumption decreased with the increase of protein level in the rations while feed consumption increased with the increase of ME level in the ration but the effect was statistically non-significant.
Maximum feed was consumed in group 6 (CP 100% and ME 120%). It was observed from the study that dietary protein had significant effect on feed efficiency and it was improved with the increase of dietary protein in the ration. Feed efficiency also improved significantly with the increase of energy in the rations. It was noted from the study that the dry matter intake reduced with the increase of dietary protein but dry matter intake increased with the increase of ME level in the ration. The effect was statistically non-significant. Protein intake was significantly increased with increase of protein level while ME level in the ration had non-significant effect. ME intake was non-significant with the increase of protein level but it was significantly increased with the increase of ME level in the ration.
The digestibility of crude protein and ether extract increased with the increase of protein and energy level in the rations but the differences were nonsignificant. The digestibility of dry matter was increased at varying levels of protein and energy but the differences were significant (P<0.0l). The digestibility of NFE and crude fibre were also increased at varying levels of energy significantly (P<0.01) but these were low at 100% protein level than 80 and 120% protein level. It was observed that the digestibility of all the nutrients increased at high plan of nutrition (protein and energy).
It was concluded that protein requirements of buffalo male calves were the same as recommended for beef cattle NRC (1976) but metabolizable energy requirements were 20% higher than the recommended level for beef cattle, NRC (1976)
Availability: Items available for loan: UVAS Library [Call number: 0348,T] (1).
44.
Comparative Study On Ordinary Alum Precipitated And Special Alum Precipitated H.S Vaccine By Dense
by Sajjad Hussain | Dr. Ata-ur-rehman rizvi | Dr. Muhammad | Dr. Muhammad naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 1994Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0351,T] (1).
45.
Sero-Epidemiological Study Of Brucellosis And Leptospirosis In Cattle And Buffaloes
by Arshad, M | Dr. Muhammad Akram Munir | Dr. Mohammad Naeem | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1991Dissertation note: Serum samples were coil collected from 400 buffaloes & 400 cattle maintained at Livestock Production Research Institute Bahadurr.aqar Okara The serum samples were subjected to serological test to detect the presence of antibodies against brucellae and leptospirae..
An over all 7.75 percent animals were found to harbour the antibrucella antibodies. The incidence by R8PT was 5.75 percent & 6,5 percent in cattle and buffaloes respectively whereas 7.0 percent and 8.5 percent samples were found positive to brucellae in cattle and buffaloes respectively by SAT.
The incidence of leptospirosis in cattle and buffaloes was recorded to he 9.75 percent and 10.0 percent respectively with an over all incidence of 9.38 percent.
Availability: Items available for loan: UVAS Library [Call number: 0354,T] (1).
46.
Comparative Study Of Oral And Parenteral Therapies Of Experimentally Induced Spirochaetosis In Broiler Chicken
by Shaif abdo Salem | Dr. Muhammad Sarwar Khan | Dr. Muhammad | Dr. Muhammad Ather Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: With rapid progress in commercial poultry farming the need to control poultry diseases, spirochaetosis is responsible for heavy economic losses with a mortalitry rate of 60 to 80 percent. (Mc Neil 1949).The disease is caused by Borrelia anserina and is of worldwide in distributed,whereever the tick Argas Percicus, which acts as the intermediate host is present.
This disease has characteristic symptoms of pyrexia, weakness,drowziness, anaemia, diarrhea, emaciation,paralysis, and finally death (Marcos et al 1946) The bfrdswhich survive remain weak,ariaemic, emaciated, and do not thrive well.This disease is of great economic importance in the poultry industry and it causes great financial ioss.It is therefore,inspective that an effective and economical treatment be devised to eradicate this problem.
This experiment.was conducted on 120 A grade day old chicks,which were reared under ideal hygienic condition.At the age of four weeeks the birds were divided into six equal groups, A, B, C, D, E and F with 20 bires in each respective group:
Group A (Non-infected , non-medicated
B (Infected and non-medicated)
C (Infected and medicated with Pencillin)
G (parenterally)
D (Infected and medicated with oxytetracyclin parenterally)
E (Infected and medicated with oxytetracyclin orally)
F (Infected and medicated with Amoxyclin Clomaxal orally)
Except for group A which was the control, all the remaining five groups showed the typical signs of the disease after 48 hours of in oculatiori.
In Group 13, the mortality rate was 40% ,Group C had a 20% mortality rate at 96 hours.Cure was l0O%.Group D had a 25% mortality and 100% cure at 96 hours post inoculation.Group E had a 30% mortality rate which was the highest of all the groups and curative rate 80%.The Group F had a 15% mortality which was the lowest record in all the six groups.It had 100% cuire at 96 hours.
From the results of this experiment it could be concluded that the 1\moxycillin (Clomoxal) was drug of choice. Penicillin G and Oxytetracycline (Terramycin orally ) showed the poorest results.
Availability: Items available for loan: UVAS Library [Call number: 0364,T] (1).
47.
A Comparative Study Of Lungworms In Sheep & Goats & The Effect Of Mixed Namatode Infection On Certain Blood Parameters
by Irshad Ahmed Sherazi | Dr. Sh,. Asif Rabbani | Dr. Muhammad | Dr. Muhammad Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: The present study was conducted to find out the incidence of lungworm infestation, identify the different species and to observe the effect of mixed nematode infections on certain blood parameters in sheep and goats. The study was conducted at Parasitology Laboratory, College of Vety. Sciences Lahore. For this purpose sheep & goats brought to Lahore abattoirs for slaughtering were closely examined before and after slaughter and animals suspected for lungworms were selected for collection of blood and tissue samples. 200 samples of lungs and blood 100 each from sheep and goats were collected for parasitological and haematolog ical examination, respectively.
The results revealed that incidence of lungworms infestation in sheep and goats was 31 and 11 %, respectively. It was observed that Dictyocaulus filaria, Protostrongylus rufescence and Muellerius capillaris infection in lung of sheep was in the order of 22, 8, and 1 % respectively, whereas, the incidence of lungworms infestation in lungs of goats caused by the same species was 8, 3 and 0 %, respectively.
The highest incidence of lungworms infection observed was that of Dictyocaulus filaria in both the sheep and goats, while Muellerius capillaris was found only in lungs of one sheep and infection by this species could not be detected in goats.
The findings of this study showed that average red blood cell count, heamoglobin level and packed cell volume were reduced due to nematode infection in sheep and goats. The results have also indicated that nematode infection in sheep and goats enhanced erythrocyte sedimentation rate. From the results of the present study, it may be concluded that incidence of lungworm infestation in sheep and goats is on the higher side which warrants that suitable treatment and control measures should be adopted to safeguard our valuable livestock from this serious infection. It is also evident from the results that nematode infection adversly affects the components of blood in sheep and goats which may lead to anaemia and loss of growth and production.
Availability: Items available for loan: UVAS Library [Call number: 0371,T] (1).
48.
A Profile Of Serum Albumin, Globulin Ratio, Total And Differential Leukocytic Count In 6-10 Months Old Buffalo Calves
by Khalid Hameed, Chughtai | Dr. M. Athar Khan | Dr. Muhammad | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: One hundred arid fifty samples of each coagulated and non- coagulated blood were collected from buffalo calves (100 healthy and 50 diseased buffalo calves) of 6 to 10 months of age
The samples were analysed for total serum protein, serum albumin, serum globulin and total leuckocytic count by applying Biuret method with commercial kit (Proti) using a spectrophotorneter, and standard techninques of Cole for total leuckocytic count.
The mean total serum protein values for healthy buffalo calves of 6 to 10 months of age were observed between 5.4 19 to 7.468 g/dl. A gradual increase in totalserum protein levels was observed with an increase of age. The total serum protein values for diseased buffalo calves varied as compared to healthy calves. A decrease in mean total serum protein levels. (6.189) was associated with malnutrition, diarrhoea, fever and Fasciollosis as compared to the mean total serum protein levels in healthy calves (6.404). An increase in total serum protein level (5.793 g/dl and 7.576 g/dl) were observed during 6th and 10th month of age as compared to healthy calves (5.419 g/dl and 7.468 g/dl). This increase was due to dehydration.
A gradual increase in mean serum Albumin levels 2.458 to 3.449 g/dl was observed in healthy buffalo calves of 6 to 10 months of age. A significant (P < 0.05) difference in serum albumin values was observed in all age groups both in healthy and diseased buffalo calves. A decrease in serum albumin levels (2.516) was observed In calves suffering from malnutrition, diarrhoea, fever and fasciollosis.
The mean serum globulin levels observed in healthy buffalo calves of 6-10 months of age was observed between 3.218 to 4.144 g/dl. These serum globulin levels showed a gradual increase with increase in age of buffalo calves. An increase in serum globulin level (3.898) was observed in diseased buffalo calves as compared to healthy calves in all age groups. The diseased calves were saffertig from malnutrition, diarrhoea, fever and fasciollosis. A significant (P <0.05) difference in serum globulin values was observed in age group of 6th month of age in healthy and diseased buffalo calves whereas in all other groups it was non-significant. The mean total leukocytic count hveIs observed in healthy buffalo calves of 6 to 10 months of age ranged between 8.765 to 9.655x103 microliter. A gradual increase iii mean total leukocytic count (9.26) x 103 microlitre was observed with an increase of age in healthy buffalo calves of 6 to 10 months of age. An increase in total leukocytic count level (10.966) was observed in diseased buffalo calves as compared to healthy calves in all age groups. A significant (P <0.05) increase in total leukocytic count was observed in calves suffering from diarrhoea, fever and fasciollosis.
From the above mentioned facts it was concluded that these blood parameters are important tools in investigation/diagnosis of many diseases. These parameters enable us to understand and compare the leveI of humoral and cellular defensive components in a natural farm co idition in buffalo calves of 6 to 10 months of age.
Availability: Items available for loan: UVAS Library [Call number: 0379,T] (1).
49.
Efficacy Of Ivermectin (Ivomec) Against Mange Mites And Warbles In Goats
by Saleem Qasur | Dr. Muhammad Sarwar Khan | Dr. Muhammad | Dr. Muhammad Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0380,T] (1).
50.
Experimental Induction Of Coccidiosis In Broiler Chicks With Eimeria Tenella And Comparative Efficacy Of Different
by Noora Jan Issot | Dr. Hajid Ahmad Hashmi | Dr. Muhammad | Dr. Muhammad Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: The study was designed to look into the immunogenic properties of Eimeria tenella. Two hundred and twenty five day old broiler chickens were reared under controlled conditions. They were equally divided into five groups i.e. A, B, C, D, and E. Group 'A' was kept as uninfected control. Group 'B' acted as infected control, which was infected with 50,000 sporulated oocysts at day 15, 25 and 35 of age. Group 'C' acted as infected and treated, which received sporulated oocysts on day 15, 25 and 35 alongwith 5 days treatment with salinomycine (60 ppm in finished feed) after 3 days of each primary and challenge dose. To group 'D' 1000 live sporulated oocysts were administered orally at day 3 and 10. Similarly, 'E' group received formalin treated 1000 sporulated oocysts orally at day 3 and 10 of age of chickens. Subsequently the members of group D and E received challenge infections of 50,000 Eimeria tenella sporulated oocysts at day 15, 25 and 35.
Immunogenicity was measured by the passage of oocysts per gm of faeces and by comparative weight gains amongst different groups. It was noticed that no coccidiosis could be established in groups A, C and E and the OPG counts remained nil throughout the period of experimnt. Group B showed high counts and group 'D' showed moderate ounts but towards the completion of experiment the OPG counts gradually reduced to 8400 and 6100 respectively, in both group.
Weight gains recorded were 473, 393, 316 and 310 gin. greater in A, E, D, and C respectively, as compared with group B. Group E depicted 1OC% protection and remained second to the uninfected control group A in respect of weight gains. Group 'D', although developed infection but due to acquired immunity became resistant to challenge infections and was placed 3rd in weight gain. Inspite of complete protection in group C due to Salinomycine treatment, the weight gains remained lower than group D and E.
Availability: Items available for loan: UVAS Library [Call number: 0381,T] (1).
