Antibiotic Resistance Profiling Of Enterococcus Faecium Recovered From Retail Chicken Meat From Lahore City
By: Amina Habib (2010-VA-313) | Dr. Ali Ahmad Shiekh.
Contributor(s): Dr. Fareeha Akhtar | Dr. Wasim Shehzad.
Material type: BookPublisher: 2016Description: 56p.Subject(s): Department of MicrobiologyDDC classification: 2489-T Dissertation note: Enterococcus faecium is gram positive bacteria which are normal intestinal flora of warm blooded animals and humans beings. It is responsible for various types of diseases such as neonatal meningitis, endocarditis, urinary tract infections and intra-abdominal or pelvic wounds. Retail chicken could be a source of Enterococcus faecium. Irrational use of antibiotics in chicken rearing can lead to emergence of antibiotic resistant Enterococcus faecium. The chicken meat gets contaminated at the time of slaughtering and resistant bacteria may transfer to human beings through food chain. In present study prevalence of E. faecium recovered from retail chicken meat samples collected from various areas of Lahore city was estimated. A total 43 chicken sample (leg or wing) were processed for isolation of E. faecium. Identification of E. faecium was made using standard culturing and biochemical reactions. Out of 43 samples, 30 samples (69%) were found positive for Enterococcus faecium. Antibiotics resistance profiling showed that the isolates were resistant to following antibiotics mentioned as below: Ampicillin (100%) >Tetracycline (73%) > Erythromycin (53%) > Ciprofloxacin (46%) > Chloramphenicol (40%) > Rifampicin and Vancomycin (36%) > Teicoplanin (33%) > Doxycycline (20%) > Fosfomycin (0%). From the study it is concluded that retail chicken is the carrier of antibiotic resistant Enterococcus faecium and could transfer resistance to humans. Efforts should be made to use antibiotics wisely and hygienic practices should be followed during slaughtering and processing of chicken meat to avoid bacterial spread from animal source to human beings.Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis | UVAS Library Thesis Section | Veterinary Science | 2489-T (Browse shelf) | Available | 2489-T |
Enterococcus faecium is gram positive bacteria which are normal intestinal flora of warm blooded animals and humans beings. It is responsible for various types of diseases such as neonatal meningitis, endocarditis, urinary tract infections and intra-abdominal or pelvic wounds. Retail chicken could be a source of Enterococcus faecium. Irrational use of antibiotics in chicken rearing can lead to emergence of antibiotic resistant Enterococcus faecium. The chicken meat gets contaminated at the time of slaughtering and resistant bacteria may transfer to human beings through food chain.
In present study prevalence of E. faecium recovered from retail chicken meat samples collected from various areas of Lahore city was estimated. A total 43 chicken sample (leg or wing) were processed for isolation of E. faecium. Identification of E. faecium was made using standard culturing and biochemical reactions. Out of 43 samples, 30 samples (69%) were found positive for Enterococcus faecium. Antibiotics resistance profiling showed that the isolates were resistant to following antibiotics mentioned as below: Ampicillin (100%) >Tetracycline (73%) > Erythromycin (53%) > Ciprofloxacin (46%) > Chloramphenicol (40%) > Rifampicin and Vancomycin (36%) > Teicoplanin (33%) > Doxycycline (20%) > Fosfomycin (0%). From the study it is concluded that retail chicken is the carrier of antibiotic resistant Enterococcus faecium and could transfer resistance to humans. Efforts should be made to use antibiotics wisely and hygienic practices should be followed during slaughtering and processing of chicken meat to avoid bacterial spread from animal source to human beings.
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