1.
Introduction to environmental impact assessment /
by Glasson, John | Ch.
Edition: 4th ed.Material type: Publisher: New York : Routledge, 2012Availability: Items available for loan: UVAS Library [Call number: 333.714 Glasson 4th 2012 29295 En.Science] (1).
2.
Science on the March, Unit One : The Air and You
by Clark, John A | Dobinson, C.H.
Edition: 2nd ed.Material type: Publisher: UK: Butler & Tanner; 1961Availability: Items available for loan: UVAS Library [Call number: 500 Clark 8569 2/e 1961 Statistics] (1).
3.
Management and People in Banking
by Ch. Mushtaq Ahmed.
Edition: 1st ed.Material type: Publisher: Lahore: Ilm-o-Irfan; 2004Availability: Items available for loan: UVAS Library [Call number: 332.10683 Mushtaq 22281 1st 2004 Management] (1).
4.
A Comparative Investigation Of The Nutritive Value Of Various Commercially Available Fish Meals In Broiler Feeding
by Anwar ul Haq, Ch | Not Available | Not Available.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1970Dissertation note: Two experimens, one of growth and feed efficiency and the other for determination of metabolizable energy were conducted on broiler chicks. Three fish meals of 55, 45 and 40 grades prepared by M/S. Pak primco Ltd. Karachi were evaluated for their nutritive values. The control ration was supplemented with meat meal at the same level.
An 8 week growth trial was conducted and broiler chicks fed on experimental rations A, B, C, and D gained average daily weight of 18.88, 18.07, 17.59 and 18.25 grams respectively. Feed efficiency for rations A, B, C and D was 3.18, 3.27, 3.36 and 3.22 respectively. When weight gain and feed efficiency data was subjected to analysis of variance, no significant difference was observed.
In the second experiment of one week duration, metabolizable energy value of experimental rations was determined. Nine week old male chicks of equal weights were maintained in battery cages in groups of two. Three groups were fed on each experimental ration. Method of total collection of excreta was adopted. Uncorrected and corrected metabolizable energy values of rations A, B, C and D per gram were calculated and found to be 3.74, 3.35, 3.20 and 3.37 Calories in the former case and 3.50, 3.12 and 3.12 Calories in the latter case on statistical analysis highly significant differences were seen between rations A--B, A--C and A--D, while non significant differences were observed between B-C, B-D and C-D rations.
Chicks fed on rations A, B, C and D metabolized nitrogen at the rate of 28.41, 28.48, 24.89 and 30.39 milligrams per gram of diet. Significant difference at 5 percent level was observed between rations D-C, because of high protein and low mineral contents of ration D than ration C.
Following conclusions can be drawn from the present research study:-
1. All three fish meals used, possessed high nutritive value.
2. Sun curing may be a satisfactory process of drying fish meals and does not deteriorate the quality of fish meals for poultry feeding.
3. Fish meal of lowest grade gave comparable results in growth, feed efficiency and metabolizable energy values with meat meal and the latter can be completely replaced without effecting the biological value of rations.
4. Complete replacement of meat meal with fih meal of lower quality will decrease the feed cost by 15-20 percent as the latter is available at approximately half of the price of meat meal.
5. Fish meals used at 7 percent level did not give any fishy odour to the meat of broilers.
6. More research work is required to evaluate various fish meals preferably at lower levels.
Availability: Items available for loan: UVAS Library [Call number: 0007,T] (1).
5.
Study On Performance Of Broilers Under Different Intensities (Wattages) Of Light And Illuminated Feeders
by Afzal Sajid, M | Ch. Muhammed Saleem | Dr. Nisar | Muhammed Aslam Bhatti.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1988Dissertation note: The study was aimed to find out the effect of different light intensities (wattages) on weight gain, feed consumption feed conversion ratio, dressing percentage, mortality and abnormalities in broiler chicks. 120, one day old chicks were selected and divided randomly in four groups of 30 chicks each. The following light treatments were provided to the chicks of the Groups A,B,C and D. (A) 40 watt bulb at night and natural day light (B) 25 watt bulb at night and natural day light (C) 15 watt bulb at night and natural day light (D) Spot-lighted feeders with 5 watt bulb at night and natural day light. Each chick was given 0.8 sq. ft. floor space. Feeding and watering were provided ad.lib. under indentical managemental conditions.
Weekly data starting from 4th week onwards revealed that at 4th week of age there wa non-significant difference in weight gain, feed consumption and feed convertion ratio among the four treatment groups. Significant differences in weight gai and feed conversion ratio and non-significant differences in feed consumption were noticed among the groups at 8th week of age.
The results indicate that birds of Group A due to more activities under high wattage gained least weight i.e. 1.6937 Kg and sonsumed more feed i.e. 4.8017 Kg and showed poorest feed conversion ratio i.eo 2.84 as compared to the birds of rest of three groups, i.e. B, C and D. The birds of Group B gained less weight i.e. 1.7343 Kg, consumed more feed i.e. 4.7983 Kg and showed poorer feed conversion ratio i.e. 2.77 than the birds of Group C and D. The birds of Group C gained less weight, i.e. 1.8293 Kg consumed more feed i.e. 4.6733 Kg and showed poor feed conversion ratio i.e. 2.55 when compared with the birds of Group D but showed better performance tha the birds of Group A and B. The birds of Group D gained significantly more weight (P<0.05) i.e. 1.8567 Kg, at low feed intake i.e. 4.6510 Kg and showed significantly better feed conversion ratio (P<0.05) i.e. 2.50 as compared to the birds of Group A and B, as the light intensity (wattage) available to the birds of Group D was very low, hence there was less activity due to less movement, as compared to Group A,B and C. Therefore, the birds of Group D converted more available energy into weight gain and showed best performance as compared to other three groups i.e. A, B and C.
Dressing percentage recorded after 8th week of age was 61.85% 62.24% 62.86% and 63.11% for Group A, B, C and D respectively. A non-significant difference was observed among all the groups.
There was no mortality throughout the experimental period and apparently no considerable abnormality was observed.
Availability: Items available for loan: UVAS Library [Call number: 0016,T] (1).
6.
Effect Of Different Freezing Levels On The Freezability Of Buffalo Bull Semen
by Javaid Iqbal Ch | Imtiaz Hussain Khan | Ata-Ur-Rehman | Rashid Ahmed Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1989Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0043,T] (1).
7.
Availability Of Calcium From Different Sources In Layers
by Riffat Hamid, Ch | Mubashar Ahmed Shah | Muhammed Nawaz Asghar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1979Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0076,T] (1).
8.
Influence Of Varying Levels Of Tallow On Economical Performance Of Broiler Chicks
by Asif Humayun, Ch | Nisar Ahmed | Muhammed | Muhammed Yaqoob Malik.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1987Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0078,T] (1).
9.
Comparative Evaluation Of Differnet Feeding Techniques And Suture Materials After Standard Esophagotomy In The Dog
by Shahid Hameed, Ch | Iqbal Ahmed | Mubasher Saeed | Zafar_ud-Din | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1989Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0103,T] (1).
10.
Proteolytic Enzyme As An Adjuvent To Antibiotic Treatment Of Surgical Wounds In Dogs
by Mukhtar Ahmad Ch | Zafar -Ud -Din Khan | Muhammed | Muhammed Tufail Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1978Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0112,T] (1).
11.
Influence Of Intermittent Periods Of Light Of Feed Conversion Ratio And Rater Of Growth In Broiler.
by Shafqat Mahmood | Ehtisham Pervaiz | Asif Rabbani | Ch. Muhammed Saleem.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1987Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0123,T] (1).
12.
Stuies On The Histopathology And Haematology Of Experimentally Induced Avian Leucosis
by Afzal Ch., M | Muhammed Tufail Khan | Not Available | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1970Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0126,T] (1).
13.
A Study On The Effects Of Natural Haemohnchosis On Total Erythrocytic Count, Haemoglobin, Serum Total Protein,
by Munawar Sultana Ch | Tufail Muhammed Khan | Asif Rabbani | Manzoor Ahmed Basra | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1989Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0130,T] (1).
14.
Bacterial Causes Of Pneumonia In Sheep
by Khadim Hussain, Ch | Dr . M . Ajmal | Mr . H . A | Nr . M . A . Majehd | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1972Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0186,T] (1).
15.
Morphological Abnormalities Of Spermatozoa In Pure Bred Friesian, Sahiwal Bulls And Friesian X Sahiwal
by Ahsan Rashad, M | Prof. Dr. Rashid Ahmad Ch | Dr. Imtiaz Hussain Khan | Dr. M. Akram | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: Of the 15 normal semen producing breeding bulls, five bulls from each of pure bred Sahiwal, Friesian and the cross-breds (Friesian X Sahiwal) of the same age group were selected. These bulls were maintained at Semen Production Unit, Qadirabad (Sahiwal) under similar conditions of housing, feeding and management. Two consecutive ejaculates once a week were collected. A total of 395 ejaculates were evaluated for various seminal characteristics, extended, processed and frozen during August, September and October, 1990. During these three months semen parameters like ejaculate volume, mass motility, percent motility and the doses produced per ejaculate declined with increase of ambient temperature and humidity.
The second ejaculate (195) at each collection and for each bull was always taken for studying the sperm morphology. Two slides from each semen sample were prepared in 10% solution of Nigrosin stain. A total of 200 sperm cells were counted in each slide and average values for various morphological abnormalities of spermatozoa in each breed were studied. The sperm cell abnormalities were accounted for head (abnormal, detached), neck (poor attachment, abaxial attachment and fractured neck), thickened mid-piece, protoplasmic droplet (proximal, distal droplet) and tail (detached, coiled- tail-droplet-enclosed, coiled tail).
The mean values of different breeds so obtained for different abnormalities during various months were analysed. The average values for head abnormalities for three breeds were 9.69 ± 1.88, 5.12 ± 060 and 1.56 ± 0.14% in Friesian, cross-bred and Sahiwal, respectively. These abnormalities were highest during August and September in Friesian bulls and lowest in Sahiwal bulls during October.
The neck abnormalities were highest in cross-breds (4.44 ± 0.57%) and lowest in Sahiwal (1.67 ± 0.14%). However, the neck abnormalities were statistically similar during the months of August, September and October. Similar trend of mid-piece abnormality was observed between various breeds and months. Likewise the values of protoplasmic droplets were highest in cross breds (14.12 ± 1.19%) and lowest in Sahiwal (2.36 ± 0.30%). The tail abnormalities were the highest in cross-breds (3.47 ± 0.45%) and lowest in Sahiwal (0.87 ± 0.12%). Among various months the tail abnormalities, were statistically similar (P> 0.05).
The total values of different sperm abnormalities wçre highest in cross-breds during August (30.93 ± 3.11%) and comparatively lower during September and October (28.66 ± 2.28 and 26.11 ± 2.63%), respectively.
Except head abnormalities, all the other abnormalities of spermatozoa were statistically significant among breeds.
Availability: Items available for loan: UVAS Library [Call number: 0206,T] (1).
16.
Immunomodulatory Effects Of Coccidiostats In Broiler Chicks
by Khalid Munir, Ch | Muhammed Ajmal | S.Ata- ur- Rehman Rizvi | tufail | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: The birds that received salinomycin at recommended dosage
levels had higher body weight than the monensin treated, cyclophosphamide treated or untreated control birds.
Both salinomycin and monensin treatments did not adversely affect the weights of bursa of Fabricius, spleen, thymus, and livers of birds. Cyclophosphamide treatment of birds in early life resulted in bursal atrophy and slight depression of splenic weight gain.
As compared to cyclophosphamide treated and non-medicated control birds, the sera of NDV vaccinated birds fed salinomycin/monensin had higher antibody titers on day 42. The post-challenge sera of NDV vaccinated birds fed salinomycin, and monensin also had higher antibody titer as compared to NDV vaccinated cyclophosphamide treated birds. The NDV vaccinated birds on salinomycin or monensin medicated rations and those on non-medicated rations did not have any post virulent NDV challenge mortality.
Availability: Items available for loan: UVAS Library [Call number: 0211,T] (1).
17.
Studies On Postpartum Buffaloes - Changes In Reproductive Organs And Onset Of Oestrus After Parturition
by Munir Ahmnad Ch | Naeem Ullah Khan | Imtiaz Hussain Khan | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1985Dissertation note: Thirty five (35) buffaloes of Nili-Ravi breed were studied for postpartum reproductive performance. Involution of the uterus and postpartum ovarian activity were monitored by repeated rectal palpations of the genitalia from day 14 postpartum until first observed oestrus. Buffaloes were checked for manifestation of oestrus for five times a day by using bulls with deviated penis. Buffaloes detected in oestrus were examined rectally and inseminated at 12 and 18 hours after the onset of oestrus.
The mean time required for complete involution of uterus was 27.5 ± 1.4 days with a range from 21 to 66 days. The rate of decrease in diameter from day 14 postpartum to complete involution was slower in non-gravid horn than in gravid horn. This overall uterine involution period was not affected by the month of calving and the age of the buffaloes but lactation number effected it significantly (P<0.06). The diameter of cervix on day 14 postpartum was 88.0 mm for buffaloes calving during March and ranged from 66 to 77 mm for buffaloes calving during other months of the study period. The rate of decrease from day 14 postpartum to complete involution was lower in buffaloes which calved in January (1.00 mm/day) as compared to buffaloes of other months (range 1.1 to 1.5 mm/day). On complete involution the diameter of cervix was 47.0 mm and it ranged 34 to 37 mm in other months of study. There was no effect of age and parity on rate of cervical involution. Similarly the rate of decrease from day 14 postpartum to complete involution, of the gravid and non-gravid horns was not effected by month of calving, age and parity of buffaloes.
The time required from calving to complete regression of corpus luteum was 20.6 ± 1.0 days. This period was found to be effected by the month of calving (P<0.06). The interval from calving to the formation of first palpable follicle averaged 27.4 ± 1.2 days. There was significant effect of resumption of follicular activity on formation of first corpus luteum (P<0.001) and first postpartum oestrus (P<0..001). The incidence of first corpus luteum formation without oestrus activity was 62.9 %. The palpable life span of first corpus luteum averaged 6.6 ± 0.7 days. The interval from calving to formation of first corpus luteum was 38.1 ± 1.5 days. It was significantly affected by the postpartum oestrus interval (P<0.009)
The average postpartum period from calving to first observed oestrus was 124.2 ± 14.5 days and ranged from 22 to 280 days. In 22.9 % buffaloes, postpartum oestrus was observed within 30 days, whereas in 57.1 % buffaloes this period was more than 120 days. This interval was highly correlated with interval to uterine involution (P<0.06) and duration of oestrus (P<0.06) . Onset of oestrus was most frequently i.e in 34.3 % buffaloes were obsered at 22.00 hours, followed by 18.00, 02.00, 06.00 and 12.00 hours in 22.9 %, 17.1 %, 14.3 % and 11.4 % buffaloes, respectively.
In contrast to onset, the termination of oestrus occurred more frequently (77.1 %) during day light hours (6 AM to 6 PM) and 6.00 PM was the time when majority of buffaloes (40 %) terminated their oestrus. The overall duration of first postpartum oestrus was 24.7 ± 1.6 hours and it ranged from 6 to 48 hours. The majority of buffaloes (65.8 %) had the duration of oestrus within 9 to 24 hours.
On the basis of 60 days pregnancy diagnosis, the conception rate to breeding at first oestrus was only 14.30%.
Availability: Items available for loan: UVAS Library [Call number: 0276,T] (1).
18.
Comparative Efficacy Of Roux-En-Y Technique With And Without Vagatomy For Pyloric Abnormalities In Dogs
by Stephen Christoper Watts | Dr.Mazhar Iqbal | Dr.Muhammad Younas Ch | Dr.Zafar Iqbal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1992Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0337,T] (1).
19.
Epidemiological Studies On Trematodes Infestation In Buffaloes In District Gujrat
by Irshad Hussain Bhatti, Ch | Khalid Saeed | Mubasher Saeed Mian | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: The study was designed to find out the prevalence and intensity of treinatodes infestation in Buffaloes in Distt. Gujrat. For this purpose twenty villages out of 1516 were randomly selected. The study period was from January to May, 1991. The data were based on:
1. Informations obtained from nine Veterinary Hospitals regarding trematodal treated buffaloes.
2. Information obtained from buffaloes owners by interogations regarding trematodes infestations in buffaloes in the selected area.
3. Findings of five hundred buffaloes faecal samples from twenty villages.
4. In order to record the actual trematodal infestations, two hundred stomach and livers alongwith their bile-ducts were inspected in three abattoirs in District Gujrat.
Data collected from Veterinary Hospitals indicated that 1252 (19.39%) out of 6554 buffaloes were positive for treinatode infection.
Informations collected from owners of the buffaloes of twenty villages showed that 133 (17.52%) out of 746 young buffaloes and 689 (21.92%) out of 3143 adult buffaloes were effected with trematodes.
Five hundred faecal samples were tested by fresh smear and sedimentation method from twenty villages and found that 144 (28.8%) buffaloes were suffering from trematodes in District Gujrat.
In order to determine the intensity of fluke disease in the infected animals, quantitative faecal examination was done and it ranged from 30 - 87.6 epg indicating that the disease is likely to be pathogenic.
Overall incidence of trematodes recorded in slaughtered buffaloes was 76.5%.
It was concluded from the findings of the study that treinatodal infestation in buffaloes in District Gujrat are quite prevalent necessitating to adopt appropriate measures to control these infestations.
Availability: Items available for loan: UVAS Library [Call number: 0340,T] (1).
20.
Study On Fasciioliasis With Estimation Of Haemoglobin & Total Serum Proteins In Camels Brought To Lahore Abbatoir
by Maj. Naeem Ahmed Janjua | Dr. muhammad Sarwar Arkhan | Dr. Ch | Dr. Muhammad Athar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: The present study was conducted to find out the prevalence of fascioliasis in different age groups of camels and to estimate the haemoglobin concentration and total serum protein levels in positive cases and also equal number of negative cases. For this purpose study was carried out on 512 camels of different age groups (calve, young and adult) brought to Lahore Abattoir for slaughtering during a period between May to July 1993.
The investigations revealed that out of 512 camels, .50 (9.76%) were positive for fascioliasis. It was observed that infection rate was higher in adult ( 10.68%) than in young (8.98%) and calf camels (7.27%). The study also indicated that the prevalence qf fascioliasis in months of May June and July was 9.20%, 10.55% and 9.46% respectively.
The present investigations reveled that an average haemoglobin concentration was 7.5 g/100 ml with a range of 4.1 - 10.3 g/100 ml (Table 8) and total serum protein level on average was 7.6 g/100 ml of positive camels with a range of 5.1 - 9.6 (Table 7).
The negative blood samples of 50 camels were also examined for the estimation of haemoglobin concentration and total serum protein levels. The mean values of haemoglobin concentration indicated by the studies was 13.1 g/100 ml ranging between 12.2 - 14.1 g/100 ml (Table 12 and 13). Total serum protein mean value revealed by the study was 6.7 g/100 ml and it ranged between 6.2 - 7.4 g/100 ml (Table 14 and 15).
Availability: Items available for loan: UVAS Library [Call number: 0367,T] (1).
21.
Comparative Study On The Incidence And Pathology Of Morbid Conditions Of Uterus And Cervix Of Teddy And Local Non Descriptive Goats
by Yousaf Hassan Khera | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Prof. Dr. Rashid Ahmed Ch | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1996Dissertation note: The project was designed to study incidence and pathology of uterine and cervical maladies in two breeds (Teddy and Local non- descriptive) of goats. Three hundred uteri and cervices (150 from each breed) of goats of three age groups, 1-2 years, 2-3 years and above three years (50 in each group) were collected from Lahore slaughter house and then thoroughly examined for any gross pathological change. The organs exhibiting macropathological lesions were subjected to morphometric, histopathological and histochemical examinations.
Of the 300 uteri and cervices, 35 (11.66%) showed morbid conditions. In 150 Teddy goats, pathological conditions were observed in, 1-2 years age group 4 (8%), 2-3 years age group 7 (14%) and in above 3 years age group 8 (16%). In 150 local non-descriptive goats, gross pathological conditions were present in, 1-2 years age group 3 (6%), 2-3 years age group 6 (12%) and in above 3 years age group 7 (14%). These results showed that incidence of pathological conditions increased with the age of the goats in both the breeds and incidence was slightly higher in Teddy goats than Local non descriptive goats in the same age groups. The following eight pathological conditions namely metritis, pyometra, pigmentation, endometrial cysts, adhesions, fibrosis, hemorrhages and aplasia of uterus were found. The morphometric, gross and histopathological changes of each disease condition of uterine horns, uterine body and cervices were studied. The histochemical evidence of tissue exhibited PAS and alcian blue reactions revealing presence of carbohydrates were also studied.
Availability: Items available for loan: UVAS Library [Call number: 0440,T] (1).
22.
Effects Of Theileriasis On Various Blood Parameters And Total Serum Protein In Exotic And Cross Bred Cattle Present In And Around Lahore
by Arshad Mahmood, Ch | Dr. Muhammad Afzal | Dr. M. Sarwar | Dr. Muhasher Saeed Mian | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: A study was conducted on Theileriasis in rfleilel.ia species, as well as haematological values were conducted by using 28 crossbred and 22 exotic cattles. Theileria annulata was the only species identified in the erythrocytes of infected cattle. Clinically, signs of panting, temperature ranging from 103.8 to 105.8°F, swelling of superficial lymph nodes, salivation and decrease in milk production were observed in infected animals.
There were significant differences in haematological values in infected cattle as compared with non-infected ones. There was decrease in total erythrocytic count, haemoglobin concentration, packed cell volume, Total leukocytic count, serum protein and neutrophils. Whereas lymphocytes showed an increase in the infected cattle.
Regarding the comparison of crossbred and exotic cattle, there was statistically significant difference in both the means of total erythrocyte count, PCV, Haemoglobin, Serum Protein, lymphocytes and neutrophils. But no significant difference was seen between means of eosinophils, Basophils and monocytes of the exotics as well as the crossbred cattle.
Availability: Items available for loan: UVAS Library [Call number: 0450,T] (1).
23.
Comparative Evaluation Of Uretero Jejunostomy & Uretero Gastrostomy In The Dog
by Nabila Yousaf Ch | Dr.Mazhar Iqbal | Dr.Muhammad | Dr.Muhammad Younis | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
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Publisher: 1993Dissertation note: Cases of rupture of urinary bladder, cystectomy for carcinoma of the bladder or urethra and extrophy of urinary bladder are all important conditions in which urinary diversions is recommended. Many surgeons in madical field as well as Veterinaians have had tried and reported various corrective surgical procedures. In the past several attempts have been made in which the bladder was excised and ureters were implanted in colon (large intestine). But satisfactory results were not obtained because the bacterial count in the colon is much higher which resulted in ascending infection towards kidneys and caused Hydro-nephritis, pylonephritis or gastro-intestinal disturbances (1979, Boree).
Beside these complications, the advantage of this surgery was that the animal would be cured from all those diseases of urinary bladder or urethra which do not respond to other form of therapy. However, the aforementioned problems of pyelonephritis etc. and the life long cover of antibiotics to combat them. Thus compels the clinician to think of some better procedures, a new project comprising ureterojejunostomy and ureterogastrostomy was designed.
Eighteen healthy dogs of both sexes were used for this experimental implantation of ureters in the stomach (pyloric antrum) into the mid and distal jejunum. The urinary bladder was emptied by gentle digital pressure and excised completely after amputating the ureters obliquely down to the trigone area. The cut ends of the ureters were wrapped in sponge moistened with saline. An entrotomy incision about 5 ems long was given on the selected segment of mid or distal jejunum 10 ems cranial to this incision a small nick in between the mesenteric and antimesenteric border was made going through the first three layers. Through the nick 1.5 cm submucosal tunnel was created going towards the entrotomy incision. The ureter was pulled into the lumen of the intestine through this tunnel. The cut end of the ureter was spatulated and sutured to the intestinal mucosa using 3/0 prolyene simple interrupted sutures. The other ureter was similarly implanted on the other side of the jejunum. With the help of a probe the patency of ureter was checked and entretomy incision was closed using 2/0 chromic catgut simple interrupted crushing sutures. Finally the abdomen was closed in routine manner.
In the dogs of group No.3 the ureters were implanted in the least vescular part of the pyloric antrum through gastrotomy incision using the same procedure explained above.
It was concluded on the basis of this study that:
1) Pressure within the jejunum and stomach interfered with drainage and forced air, food particles and organisms up to the renal pelvis.
2) As the stomach and jejunum were acting as reservoir and the urine was in contact with the wall for a longer period, reabsorption of urinary solutes occured and resulted in azotemia.
3) The typical changes were hyperchloraemic acidosis with potassium depletion and this occured in every patient with uretro-jej unostomy and uretero-gastrostomy diversion.
4) When severe the patient developed loss of appetite, weakeness, thirst, listless, coma and died.
Availability: Items available for loan: UVAS Library [Call number: 0470,T] (1).
24.
Effects Of Two Anticoccidial Drugs With And Without Supplementation Of Vitamins On The Immune System Of Broiler Chickens
by Nazim ud Din | Dr.Khalid Munir Ch | Dr.Muhammad | Dr.Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1998Dissertation note: This project was designed to investigate the effects of two anticoccidials Ampisol 20% and Esb3 30% with and without supplementation of vitamins (A, D3, E and K3 as "Symodek") on the immune system of broiler chickens. To evaluate such effects the parameters used for study were; effects, on the weights of immuno regulatory organs (bursa of Fabricius, thymus, spleen and liver), organ body weight indices, histopathology of primary lymphoid organs (bursa of Fabricius and thymus), total and differential leukocytic counts, total serum protein, albumin globulin ratio and immune response to Newcastle disease virus vaccination. A total of 120 chicks, divided into 5 equal groups viz A to E were reared for 42 days. Groups A, B, C, and D were medicated with, Ampisol 20%, Ampisol 20% + Symodek, Esb3 30%, and Esb3 30% + Symodek, respectively while group E was kept as control(no drug medication). All the birds were vaccinated with ND virus vaccine on day 7th and day 2 1st. All the medication, was done through drinking water at the recommended prophylactic dosage levels.
The study revealed that Ampisol 20% had favourable effects on the weights of immunoregulatory organs, and organ (bursa of Fabricius, thymus, spleen and liver):bodyweight indices. Esb3 30% had negative effects on these parameters.
Histological studies of primary lymphoid organs (bursa of Fabricius and thymus) revealed no detrimental effects of different medications except in birds of group C and D given Esb3 30% or Esb3 30% + Symodek, where slight degenerative changes were noted in bursae and thymi.
On day 42 the mean TLC values were slightly lower in birds medicated with Esb3 30% as compared to birds which received Ampisol 20%. On day 42 the mean lymphocytic percentages were higher in groups medicated with Arnpisol 20% as compared to those given Esb3 30%. Vitamins supplementation exerted favourable effects on TLC and DLC values. This study further indicated that mean total serum protein (TSP), mean serum albumin, mean serum globulin and mean A/G ratios were higher in birds medicated Ampisol 20%, than those medicated Esb3 30%. Vitamin supplementation had augmenting effects on these values. On day 28, 35 and 42 the antibody titres in birds medicated with Ampisol 20% were higher than those given Esb3 30%. Vitamin supplementation exerted augmenting effects on the antibody titres.
It is concluded that Ampisol 20% had no immunosuppressive effects rather it exerted favourable effects on the weights of immunoregulatory organs, rpp albumin, globulin, A/G ratio, DLC and HI antibody titres against Newcastle disease virus. Esb3 30% moderately depressed the aforesaid components of immune system. The present study also revealed that vitamins (A, D3, E, K3) supplementation to anticoccidials improved the immune response of birds. However, there is a need for further investigation in this regard, with special reference to immune system of broiler chickens.
Availability: Items available for loan: UVAS Library [Call number: 0579,T] (1).
25.
The Effect Of Different Space Densities And Addition Of Zinc Bacitracin On Growth Rate And Total Serum Protein Level
by Imran Wazir, M | Dr.Muhammad Saleem ch | Prof.Dr.Muhammad Aslam Bhatti.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1998Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0584,T] (1).
26.
A Study On The Prevalence Of Disease And Other Problems Related To Managemental Practices During
by Akhtar, M | Dr.Muhammad Saleem Ch | Prof.Dr.Muhammad Aslam Bhatti.
Material type: ; Format:
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Publisher: 1999Dissertation note: A study on the prevalence of diseases and other problems related to the managemental practices during brooding in broilers was carred out in Muzaffarabad and Mirpur districts of Azad Jammu and Kashmir during the months from May, 1999 to September, 1999. The data was collected through a questionnaire (Appendix-i) from 60 farmers i.e. 30 from each district selected randomly. Various parameters regarding the farming structure in the project area, location and dimensions of farms/sheds, brooding management, feed and feeding, watering, lighting schedule, equipment, litter managemental practices, biosecurity and sanitation, vaccines and vaccination, disease problems during brooding, health cover, mortality, relative humidity, ventilation control, purchase of day old chicks, extension services availability, labour management and farmer's suggestions for broiler farming improvement, were studied.
Mostly the farmers (55%) were having an educational level upto Matric and a few farmers were above Matric. Construction of sheds differed in Muzaffarabad and Mirpur districts due to different climatic conditions and availability of materials. Most of the farmers(61.7%) had no separate brooder houses and those who had brooder houses did not give attention to ventilation system. There was lack of knowledge about advanced brooding managemental practices among the farmers. Improper feeding and watering space was given to chicks during brooding. Disease outbreaks were observed even after vaccination due to poor vaccination practices. Lack of sanitation and biosecurity resulted in outbreaks of many diseases such as omphalitis, ]. ij, Gumboro, coryza, Newcastle Disease etc. at many farms during brooding period. The farmers mostly depended upon labour and did not manage their farms, which resulted into losses. Most of the farmers blamed market fluctuation, while some blamed the low quality of feed, chicks and vaccines which resulted in decline in poultry farming.
Availability: Items available for loan: UVAS Library [Call number: 0611,T] (1).
27.
A Study On The Surveillance Of Management Related Problems In Broiler Farms Of Lahore District
by Irfan Shahzad Sheikh | Dr.Muhammas Saleem Ch | Prof.Dr.Muhammad Aslam Bhatti.
Material type: ; Format:
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Publisher: 1999Dissertation note: A survey was conducted to collect the data from 150 randomly selected broiler farms, through a questionnaire to evaluate the surveiling management related problems and present status of broiler farms of Lahore district. It was observed that of the total 150 respondents 4.6% were found to be illiterate whereas majority of the farmers i.e. 58.7% were of below matric education level. 46.7% farmers took broiler farming as part time business. 57.33% farmers had no agriculture land and 42.67% had their own land.
Most of the farmers (52.%) kept single shed only and maximum number of farms (46.7%) were raising 2001-5000 birds with their current strength of 50%. Of the total farms, 60 (40%) farms were rented and 90 (60%) farmers had their own farms.
It was noticed that 63.34% farms were located within the range of 25-40 kms of Lahore city and 94.67% farms had a main road facility within 5 km of range. At 14.66% farms boundry walls were present. It was also observed that there was no trend of homernix and automatic feeding in broiler farming. It was found that 87.3% farmers purchased feed on credit, 70.67% farmers practiced ad-libitum feeding programme and 61.3% farmers stored feed inside the sheds.
It was seen that at 8.57% farms proper water storage facility was not available. E. coli was observed at 42.0% farms and 9.3% farmers were using manual waterers. It was also revealed from the data that out of 150 farms 16.0% were using tubelights, 68.7% used bulbs whereas 15.3% were using lanterns, gas lamps etc. due to nonavailability at their farms. It was seen majority of farmers (89.3%) used wood and/or coal brooder during brooding.
It was observed that 48.8% farmers used rice husk, 30% saw dust, whereas 28.7% were of in favour of using sand in summer season, but there was no use of sand in winter season. Most of the farmers (82.7%) were aware of racking and change of litter material and 62.7% farmers used litter material from 1-2".
At all farms there was a strong labourer interaction, no labour disinfection was practiced. There was no restriction to farm visitors and at 8% farms other livestock was also present. At all farms vaccination was done by feed company vaccinators and role of private manifactured vaccines was observed to be 86.67%. It was noted that 49.3% farmers received 98-99% A grade chicks, whereas 92.67% farmers took help of middlemen in marketing of their birds.
Gumboro ranked first in prevalence of disease with a percentage of 52% and most of the farmers (64.1%) had mortality percentage ranging from 6-15%. It was find out that 83.33% farm labourers had charges in a range of 2001-2500 rupees and 82.6% labourers were raising from 1600-2500 birds. It was also found that 75.33% farmers had feeding cost from 65-70%. It was also evident that 86% farmers had vaccine and medication cost upto 5 rupees per bird. It was noted that almost at all the farms technical services were provided by the Technical staff of Feed Mills, Hatcheries and Pharmaceutical companies.
Availability: Items available for loan: UVAS Library [Call number: 0612,T] (1).
28.
A Study On The Effect Of Gnrh Analogue On Anovulation In Repeat Breeding Cross Bred Cows And Their Conception Rate
by Asif Rafiq | Prof.Dr.Rashid Ahmed Ch | Dr.Imtiaz Hussain Khan | Dr.Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1999Dissertation note: The incidence of repeat breeding is high in cross-bred cows due to low LH level in the blood at the time of estrous. In this study Dalmarelin a GnRH analogue which induces ovulation in cows was used. Nine healthy animals with the history of repeat breeding and clinically normal genital tract were selected at the Livestock Production and Research Institute, Qadirabad, Okara. These animals were injected with dalmarelin (Lecirelin), at the dose rate of 2 ml IM. The injection was given on the zero day of estrous and later animals were inseminated artificially. Two blood samples were collected from these animals, one before the injection and artificial insemination i.e. on the zero day of estrous and the second at 10th day after the injection of Lacirelin for the detection of plasma progesterone concentration. Results indicated that five animals i.e. 55.5% had conceived. Similarly when detected 12 hours after the end of estrous five animals had ovulated out of which three animals were conceived. Finally, these animals were palpated rectally on the 90th day after artificial insemination and five animals with high concentration of progesterone on the 10th day, were, detected positive for pregnancy. None of the control animals ovulated and conceived.
Availability: Items available for loan: UVAS Library [Call number: 0623,T] (1).
29.
Concentration Of Serum Electrolytes In Buffaloes In The Late Pregnancy Parturition & Postparturition Periods
by Shahzad Hussain | Dr.Muhammad Amir Saeed | Dr.Muhammad | Prof.Dr Rashid ahmad ch | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2000Dissertation note: This study was executed on 30 pregnant Nili-Ravi buffaloes. Serum was harvested for the estimation of calcium, magnesium, phosphorus, sodium, potassium and iron in the late pregnancy (at 8t5 and 9th month of gestation), at parturition and in the postpartum periods (at 1st and 2" month postpartum). The mean values of Ca were 9.30, 7.95, 6.90, 8.43 and 7.33 mg/dl and of Mg were 2.38, 2.18,.2.81, 2.61 and 2.50 mgldl of serum in the above mentioned 5 stages, respectively. The mean concentration of P was 6.98, 4,15, 3,177, 4,76 and 5.38 mg/dl, respectively. The mean concentrations of Na were 147.14, 145.71, 146.50, 141.37 and 137.69 mmol/1 and of K were 4.90, 4.53, 4.31, 4.53 and 4.38 (mmol/1) of serum, respectively. The mean value of Fe was 269,36, 225.58, 212.82, 260.77 and 282.31 pg/dl of serum, respectively. The concentrations of Ca and P were significantly (P< 0.05) lower at the time of parturition. The value of Mg was significantly (P<zO.05) higher at the time of parturition. The concentrations of Na and K did not show much variation. The values of Fe also decreased significantly (P<0.0S) near parturition (at month of gestation) and at parturition. No puerperal disorder was observed in any of the experimental animals. Hence, the data of normal electrolyte values near parturition, at parturition and following parturition was obtained.
Availability: Items available for loan: UVAS Library [Call number: 0628,T] (1).
30.
Use Of Gnrh To Improve The Conception Rate Of Nili Ravi Buffaloes During Low Breeding Season
by Ghulam Ahmad | Dr.Muhammad Ahmad Ch | Dr.Masood | Prfo.Dr.Rashid Ahmad Ch | Faculty of Veterinary Sciences.
Material type: ; Format:
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not fiction
Publisher: 2000Dissertation note: In this study the main objective was to improve the conception rate of repeat breeder Nili-Ravi buffaloes during low breeding season by the use of GnRH (Dalmarelin) . For this purpose 21 .repeat breeder Nili-Ravi buffaloes were randomly selected and equally divided into three groups (A, B and C) Group A was treated with 2 ml Dalmarelin IM at the time of insemination, group B was treated with lml of Dalmarelin IM at the time of insemination and imi twelve hours of post insemination, while group C (control) received an injection of 2m1 normal saline IN at the time of insemination. The mean progesterone concentration in group A was 0.95 ng/ml, group B, it was 0.94 ng/ml and in group C, O.93ng/ml on the day of estrus (day 0), while progesterone concentration of group A was 1.16 ng/ml, group B, it was 1.089 ng/ml and in Group C, it was 1.07 ng/ml on the 10th day of estrus cycle. The conception rate was recorded as 42.8% in group A, 28.6% in group B and 14.3% in group C.
Availability: Items available for loan: UVAS Library [Call number: 0630,T] (1).
31.
Comparative Efficacy Of Craniodorsal & Ventral Surgical Approaches For Femoral Head And Neck Excision In Dogs
by Aamir Aslam, Ch | Dr . Muhammad Arif Khan | Dr . Asim Aslam | Dr . Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2000Dissertation note: There are different joint problems in dogs but out of all the incidence of hip joint problems is higher. There are different problems of hip joint mainly, hip dysplasia, leg-calve-perthes disease, degenerative joint disease, irreparable fracture of acetabulum, femoral head and neck. Different methods are used as a treatment for above mentioned ailments. The methods used for such type of problems are categorized into conservative, palliative and surgical.
The surgical treatment is further divided into (1) salvage procedure i.e. femoral head and neck excision (2) total hip replacement.
The present project was launched to find out the efficacy of two approaches used for the femoral head and neck excision in dogs. The study was conducted on 18 mongrel dogs of either sex ranging in weight from 20.25 kg, which were divided into three groups of 6 dogs each.
In group A femoral head and neck excision was performed by using craniodorsal approach, whereas in group B ventral approach was used for the same procedure. Group C was kept as control in which the femoral head was luxated by using either approach. All the dogs were kept in kennels for a period of sixteen week and results were collected on the basis of clinical and radiographic evaluations. All the dogs were euthanized at the end of experimental period and operation sites were explored to find out the gross changes and also to find out the evidence of false joint formation in the muscles.
The analysis of results clearly indicated that craniodorsal approach was a better procedure than ventral approach for the femoral head and neck excision in dogs.
Availability: Items available for loan: UVAS Library [Call number: 0667,T] (1).
32.
Serological Surey Of Brucellosis In Man & Various Age Groups Of Cattle And Buffaloes
by Abbas Chaudhry, M | Dr.Irshad Ahmad Ch | Dr.Imtiaz Hussain Khan | Prof.Dr Akram | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: The sero-prevalence of brucellosis in cattle, buffaloes and various personnel employed at Livestock Production Research Institute, Bahadar Nagar, District Okara, was surveyed. Four hundred and seventy blood samples of different age groups of various groups of cattle, buffaloes and man were analyzed for the seroprevalence of Brucellosis. The various serological tests used for this investigation included the Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT). The higher incidence of the disease was observed in cattle than buffaloes. The seroprevalence of disease in man was found to be related with the prevalence of Brucellosis in animals.
The overall prevalence of Brucellosis in clinically healthy buffaloes and cattle was found to be 7.64% and 10.0% respectively, by SAT and 11.17% and 14.11% respectively by RBPT.
A prevalence of 36.67% by RBPT and 26.67% and 23.33% by SAT in clinically sick buffaloes and cattle was recorded.
The serum samples from workers subjected to RBPT and SAT revealed prevalence of 15.71% and 11.43%, respectively. The incidence of Brucellosis in adult buffaloes and cattle above 2-10 years of age was 11% and 13%, respectively by SAT. Whereas in young buffaloes and cattle under 2 years of age, it was found to be 2.86% and 5.71%, respectively by SAT. The RBPT indicated 15% and 5.7 1% incidence of the disease in adult and young buffaloes, respectively.
Similarly prevalence of 19% and 7.14% in adult and young cattle, respectively was found to be demonstrated by RBPT. rpIe SAT and RBPT established a greater incidence of brucellosis in female animals than male cows 10.67% and buffaloes 8.67% by SAT.
Availability: Items available for loan: UVAS Library [Call number: 0673,T] (1).
33.
A Study On The Effects Of Different Photperiods On The Performance Of Coturnix Coturnix Japonica (Japanese Quails)
by Khalid Mahmood | Ch Muhammad Saleem | Dr Muhammad Aslam Bhatti | Dr Saghir.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1985Dissertation note: The study was aimed to find out the effect of different photoperiods on body weight gain, feed consumption, feed conversion ratio, age at first egg laid, mortality and dressing percentage of Japanese quails (coturnix coturnix Japonica) . The photoperiods provided were natural day light, 6 hours light and 18 hours darkness, 10 hours light and 14 hours darkness and 14 hours light and 10 hours darkness to groups A, B, C and D respectively.
One hundred and eighty, one day old quail chicks were divided into four groups comprising 45 birds each. These groups were further sub-divided into three replicates of 15 birds each. They were provided 25 sq. inches floor space per bird and fed ad-libiturn on a ration containing 26% protein throughout the study period of 70 days. Feeding, watering, environmental and manage mental conditions were made identical for all the birds. The average live weight of the quails recorded at the end of the 10th week was 161.25 gms., 134.52 gms., 165.59 gms., and 186.33 gms., with feed consumption of 870, 699, 882, and 1031 gms., for the groups A, B, C and D respectively. A highly significant difference was observed for weight gain among all the groups except groups A and C. For feed consumption a highly significant difference (P/0.0l) was observed in groups B and D while the difference in groups A and D was significant (PL0.05) and no significant difference was noticed in groups A and C, because of almost equal hours of light. The feed conversion ratio was 5.68, 5.53, 5.60 and 5.79 for groups A, B, C and D respectively, and no significant difference was observed among all the groups.
The age in days at which the first egg laid was 55, 69, 58 and 51 in groups A, B, C and D respectively. The mortality percentage was 16.00, 33.55, 13.33 and 11.00 and the mean dressing percentages recorded at the end of experiment were 62.5, 63.0, 62.7 and 64.0 for groups A, B, C and D respectively. No significant difference was observed for dressing percentage in all the groups.
CONCLUSION
From the results of the present study it was concluded that Japanese quails can be reared economically and efficiently under natural day light throughout the year in Punjab for table purpose. It is advisable to market the broiler quails at 6 weeks of age as keeping them longer is uneconomical.
However, if the quails are to be kept for breeding purpose, at least 14 hours continuous light per day should be given in order to attain early sexual maturity and onset of egg production.
It is suggested that the study should be extended upto two successive generations to assess the productive potential of Japanese quails in detail under local environmental conditions of Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 0784,T] (1).
34.
Comparativge Efficacy Of Gnrh And Pgf2A For Oestrous Synchronization In Nili Ravi Buffalo
by Abdohoo Abdali, M | Dr.Imtiaz Hussain Khan | Dr.Masood | Dr.Rashid Ahmad Ch | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: Buffaloes occupy an important role in agricultural economy of Pakistan as a triple purpose (milk, meat and draught) animals. Total population of buffalo in Pakistan is 21.6 million. '1'oal milk production in the Pakistan is 24,874 metric tons, out of which 16,665 in Punjab and the share of buffalo is 72.12%.
In buffalo husbandry seasonality in breeding is a problem of great importance, because seasonal variation in breeding, as its effects on calving profoundly affects the economy of milk production also.
The appearance of oestrous in buffalo is at the maximum (37.56%) during October to December. Therefore, population of the country parturate from July to October. Eventually, during the summer months there is a marked decline in total milk production but demand of milk escalates.
To device ways and methods to overcome summer shortage of milk we designed this project to change the breeding pattern of buffaloes by oestrous cycle synchronization in a way to have maximum milk yield during summer months.
In this study 30 non-suckling postpartum Nili-Ravi buffaloes were used having average age 2592.66±127.41 days and average weight 464.7U,±8.27 kgs. The 30 buffaloes were randomly divided into two groups viz group A and B, having 15 animals in each group respectively. The buffaloes in group A (n=15) received intramuscular injection of Dalmazine, analogue of PGF2 a 2CC, without palpation at 11 days interval.
In group B (n=15) received intramuscular injection of Dalmarelin, Analogue of GnRH 2CC without palpation at 11 days interval.
All the animals were observed for (a) Response of treatment (b) interval to the onset of oestrous post-treatment (c) Numbers of buffaloes showing symptoms of oestrous (d) duration of oestrous (e) conception rate (0 cost comparison of both injections per animal.
From the present experiment it was observed that, the response of oestrous in group A was 86.66% and in group B was 80%, the interval of oestrous in group A was 3.53±0.32 days while in group B was 12.5±0.34 days. The duration was 21.69±0.53 hours in group A and 20.9±0.67 hours in group B. The symptoms was frequent urination, bellowing, segregation from the herd, acceptance of teaser bull, vulvular sniffing, vulvular swelling and mucous discharge in both groups. The conception rate was 76.92% and 75% in group A and B respectively. The cost comparison was Rs.420 in group A and Rs.452/animal in group B which was more in group B, I .e. Rs.32/animal. It was concluded that PGF2 is more effective for oestrous synchronization, by less time, high conception rate, high percentage of response. However, a detailed study is required to determine the blood hormonal levels after the induction of both the injections, for better improvement.
Availability: Items available for loan: UVAS Library [Call number: 0796,T] (1).
35.
Mercury Intoxication In Grass Carp (Ctenopharyngodon Idella)
by Shehar Yar Khan, M | Dr.Zafar Iqbal Ch | Dr.H.A.Hashmi | Dr.Muhammad Nadeem Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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not fiction
Publisher: 2003Dissertation note: The present project was carried out to study the effects of mercuric chloride (HgCI2) toxicity in grass carp (Ctenopharyngodon idella). For this purpose 360 fish were procured from local fish farm and after one week of acclimatization, lethal concentration 50 (LC50) of HgCI2 was determined. Follow this two experiments were conducted to study viz, acute and chronic effect of mercury poisoning. For acute phase experiment 48 fish were divided into four equal groups (A, B, C and D). Groups B, C and D were given HgC12 at sublethal dose rate of 0.4, 0.5 and 0.6 mg/L, respectively. Group A acted as control group. Skin, gills and kidneys were collected after 48 and 96 hours for histopathological study. For chronic phase experiment 72 fish were divided into five equal groups (E, F, G, H and I). Groups F, G, H and I were given HgCI2 at sublethal dose of 0.3 nig/L for 4, 8, 12 and 16 days, respectively. Group E acted as control group. Skin, gills and kidneys were collected from each group after 4, 8, 12 and 16 days for histopathological study. In mercury exposed groups normal morphology of epidermis was disturbed and number of immature cells were increased. Overall skin layers were atrophied and withered. Histopathology of gills showed hyperplasia of epithelial cells of gill filaments, fusion of secondary lamellae giving a club shaped appearance of filaments. On certain areas there was sloughing of respiratory epitheliurn in mercury exposed groups. Histopathological examination of kidneys also showed a wide range of toxicity lesions. Certain areas of kidney showed disintegration and disorganization of cells of both renal and interstitial tissue and consequent dilatation and thickening of glomeruli and renal tubules. Mild to sever tubular epithelial degeneration, and necrosis was also evident. In chronic phase experiment effects of HgC12 on clinical signs and growth rate of fish were also studied. There was suppression of growth in chronic exposure groups (G, H, & I). Restlessness, difficult breathing, fin flickering and jerky movements were also observed in treatment groups. Suppressed growth rate was observed. Therefore, it was concluded that mercury intoxication not only resulted in marked histopathological changes and abnormal clinical signs but also depressed the growth rate.
Availability: Items available for loan: UVAS Library [Call number: 0800,T] (1).
36.
Effect Of High Dietary Calcium And Low Phosphorus On Urinary System Of Broiler Chicks
by Majeed Ansar | Dr. Shakil Akhtar Khan | Dr.Nisar | Dr.Zafar Iqbal Ch | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2003Dissertation note: The present study was carried out to examine the effect of high dietary calcium and low phosphorus on urinary system of broiler chicks. A total of ninety (day-old) broiler chicks were divided into3groups designated as A. 13 and C. The birds were given starter feed in the first 3 weeks of their life and the finisher feed in the remaining 3 weeks of the experiment. The calcium phosphorus ratio in the feed for groups A, B and C was 1 : 0.5, 2: 0.5 and 3 : 0.5 respectively, throughout the experiment. The birds of group A served as control. Various parameters studied included, determination of serum calcium and phosphorus concentrations, estimation of Feed Conversion Ratio (FCR), kidney body weight ratio, gross pathological examination of urinary system and histopathological examination of kidney.
The birds of groups B and C, fed high dietary calcium and low phosphorus revealed hyperealcaemia and hypophosphataemia in their sera. The FCR values of groups A, B and C, came to be 1.728, 1.876 and 2.089 respectively, reflecting inferior feed conversion efficacy of treatment groups as compared to the control one. The mean kidney body weight ratio of groups A, B and C was 9.41, 11.95 and 16.08 respectively, thereby indicating adverse effects of high calcium and low phosphorus on kidney body weight ratio of experimental birds. The urinary system of the experimental birds, in general, displayed inflammatory lesions, showing abnormalities of colour, size, shape and texture of kidneys and ureters. The latter also experienced occlusion and distention. The microscopic examination of the experimental birds showing gross pathological lesions revealed common hostopathological changes, involving glomeruli, tubular cells and interlobular veins, comprising increased cellularity, degeneration, haemonrrages etc.
The findings of the present study have shown the adverse effect of high dietary calcium and low phosphorus on urinary system of broiler birds. It is therefore, suggested that for obtaining optimal growth and performance of broilers, a diet with calcium and phosphorus ratio of 1.0 : 0.5, may be formulated.
Availability: Items available for loan: UVAS Library [Call number: 0808,T] (1).
37.
Study On The Experimental Inoculation Of Mycoplasama Gallisepticum In Chemically Immunosuppressed Broiler
by Hassan Mujtaba Naqvi | Dr.Zafar Iqbal Ch | Dr.Masood Rabbani | Prof.Dr.Ikram | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2003Dissertation note: The project was carried out to study the effect of Mycoplasma gallisepticarn infection in chemically immuno-suppressed and normal broiler chicks by recording weight gain, calculating feed conversion ratio, detecting the immune response against Newcastle disease virus (NDV) vaccine and observing the pathological changes in lungs at gross and histopathological levels.
For this study 180 day-old broiler chicks were purchased. The birds were divided into three equal groups i.e. A, B and C having two replicates in each group. The birds of group A were subjected to immunosupression using cyclophosphamide and challenged with M. gallisepticum at day 14 of age. The birds of group B were not immuno-suppressed and only challenged with M. gallisepticum at the same day while the group C was kept as control. Weight gain and feed conversion ratio was studied at day 14 of age before inoculation and at days 24, 34 and 44 of age after the inoculation. The blood and lungs samples from each group were also collected at day 14 of age before inoculation and at days 24, 34 and 44 of age after the inoculation for detection of immune response against NDV vaccine and for observing the pathological alterations, respectively.
Lowest weight gain was recorded in birds of group A which was both immuno-suppressed as well as infected with M. gallispecticum (PcO.05) amongst the three treatment groups.
Similarly, feed conversion ratio of the group A was significantly lower (P<O.05)than those of group B and group C.
Geometric mean haemagglutination inhibition (GMHI) titers against NDV vaccine of immunosupressed and M. gallisepticum infected birds was lower than those of M. gallisepticum infected and control birds.
Gross pathological findings of the lungs of infected birds revealed congestion, consolidated spots, haemorrhages and accumulation of frothy material. The bronchi of the infected birds showed caseous exudates. Histopathological changes in lungs of infected birds depicted odema, hyperplasia of alveolar walls with massive mononuclear cell infiltration. This mononuclear cell infiltration was also seen in alveoli of infected lungs. However, these gross and histopathological changes were more severe in group A (immunosuppressed and M. gallisepticum infected) than that of group B (M. gallisepticum infected). No such gross or histopathological changes were observed in the control group C which was neither immunosuppressed nor infected with M. gallisepticurn.
Availability: Items available for loan: UVAS Library [Call number: 0825,T] (1).
38.
Prevalence And Chemotherapy Of Balantidium Coli In Cattle Around River Ravi Bank Lahore
by Ch. Qasim Bilal | Prof. Dr. Khalid Pervez | Dr. Muhammad Sarwar Khan | Prof. Dr. Haji | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2006Dissertation note: The present project was designed to find out the prevalence of Balantidium coli and to evaluate the efficacy of Terramycin, Dysen forte, Flagyl against naturally occurring balantidosis in cattle under field conditions around the river Ravi bank Lahore. The faecal samples from animals were collected directly from rectum of animals and brought to the laboratory of Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore for coprological examination.
For this purpose two hundred cattle were examined coprologicaly around the bank of river Ravi in Lahore. Out of 200 cattle, 50 were positive, so the prevalence of Balantidium coli in cattle was 25%. Out of 50 positive animals thirty two cattle having balantidiosis were selected for chemotherapeutic trials and eight non effected cattle were selected and divided into five groups viz. A, B, C, D and E comprising eight animals each. The animals of Group-A were treated with oxytetracycline capsules (Terramycin) @ 8mg/kg orally, Group-B with secnidazole tablets (Dysen forte) @ 10mg/kg orally, Group-C with metronidazole tablets (flagyl) @ 25mg/kg orally. The animals in Group-D were serving as infected untreated control group and Group- E were serving as untreated healthy control.
The efficacy of drugs was evaluated on the basis of disappearance of clinical signs and by reduction in number of cysts/trophozoites on zero, 3, 7 and 1 0 day post medication. Efficacy of oxytetracycline (terramycin) was 25%, 50% and 62.5% on 3, 7 and 10 day, respectively. Efficacy of secnidazolc (dysen forte) was 37.5%, 75% and 87.5% on 3, 7 and 10 day, respectively. Efficacy of metronidazole (flagy) was 12.5%, 25% and 37.5% on 3, 7 and 10 day, respectively.
It was concluded from the above results that Dysen Forte was more effective than Terrarnycin or Flagyl. However, Terramycin showed better efficacy than that of Flagyl.
Availability: Items available for loan: UVAS Library [Call number: 0927,T] (1).
39.
Molecular Detection Of Babesia Bigemina And Babesia Bovis In Carrier Cattle By Duplex Polymerase Chain Reaction
by Muhammad Suleman | Prof. Dr. Zafar Iqbal Ch | Dr.Asim Aslam | Prof. Dr Abdul Rauf Shakoori | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 2006Dissertation note: Babesiosis is a highly important disease in the world, caused by the intraerythrocytic protozoan parasites of the genus Babesia. A wide range of domestic and wild animals and occasionally man are affected by this disease, which is transmitted by ticks and has a worldwide epidemiological distribution. While the major economic impact of babesiosis is on the cattle industry, infections also occurs in other domestic animals , including horses, sheep, goats, pigs and dogs.
The present study targeted the carrier cattle infected with Babesia bigemina and Babesia bovis, as they are difficult to detect because of the low numbers of parasites that occur in peripheral blood. However, diagnosis of low-level infections with the parasite is important for studying the transmission and standardizing epidemiological studies. Using the Polymerase Chain Reaction (PCR) to amplify a portion of the gene from the parasite, and tested the ability of this method to detect carrier cattle.
A study was conducted to detect the. Babesia in blood samples through PCR based techniques. A PCR assay was described which could differentiate Babesia bigemina and Babesia bovis by using specific primer in carrier cattle. Blood samples of 100 cattle were randomly analyzed with PCR assay 29 (29.0%) out of 100 blood samples were positive for babesiosis in which 18% were positive for Babesia bigemina and 11% were positive for Babesia bovis, While the Light Microscopy detected only 18 (18%) out of the same samples. The samples found positive by LM were reconfirmed during the PCR assay but no sample was found to be having both Babesia bigemina and Babesia bovis infections simultaneously.
Thus it is concluded that PCR is a reliable molecular diagnostic technique to detect low level of infections in carrier animals in a population and thus could be used as an effective screening tool for the control and eradication of disease.
Availability: Items available for loan: UVAS Library [Call number: 0929,T] (1).
40.
Standardization Of Avian Leukosis Diagnostic Techniques Through Polymerase Chain Reaction (Pcr) And Confirmation With Enzyme Linked Immunosorbant Assay (Elisa)
by Abdul Razzaq (M.Phil) | Prof. Dr. Zafar Iqbal Ch | Mr. Asim Aslam | Prof. Dr.Abdul Rauf Shakoori | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2006Dissertation note: Avian Leukosis Virus type J infection of chickens is a neoplastic disease affecting chickens. ALV-J is of great economic significance not only because of tumor mortality, but also because of decreased egg production in meat breeding stocks, increased rate of infections, poor response to vaccination and weight suppression in broilers. There is wide spread prevalence of ALV-A and ALV-J in commercial chicken flocks. For control of ALV's eradication programmes based solely on dam testing may be less effective than those where dam testing is combined with procedures to mitigate early horizontal transmission in progeny chicks. For this purpose PCR along with antigen capture ELISA was used in combination for detection of ALV-J proviral DNA, and ALV group specific antigen i.e. p 27 antigen of ALV-J. Polymerase chain reaction technique was standardized by using improved version of H7 primers specific for ALV sub group J targeting env gene encoding gp85 for the detection of avian leucosis virus type J and its confirmation was carried out by comparing it with antigen capture immunosorbant assay which measures group-specific antigen (GSA) i.e. p27 antigen. Feather pulp and serum samples from 50 broiler birds of up to 7 weeks of age were randomly selected from 10 different broiler poultry farms of district Lahore Pakistan. The prevalence of ALV-J was 22 % for antigen capture immunosorbant ELISA and 34 % for polymerase chain reaction (PCR).
Availability: Items available for loan: UVAS Library [Call number: 0943,T] (1).
41.
Comparative Efficacy Of The Transverse Pylorpplasty And Y-U Advancement Pyloroplasty As A Relief To Pyloric Stenosis in Dogs
by Ayesha Safdar Ch | Dr.Muhammad Arif Khan | Dr.Asim Khalid Mehmood | Prof.Dr..Zafar | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2007Dissertation note: Stomach is important and biggest dilatation of alimentary canal which mainly serves the purpose of digestion of food. The stomach has two extremities cardia and pylorus, it also has fundus and body. The pyloric extremity is connected with the duodenum by pyloric canal. The gastric emptying of food is controlled by pyloric sphincter. Many problem have been diagnosed in the pylorus such as gastric and duodenal ulcers, neoplaisa, chronic gastritis, drugs and chemicals resulting in pyloric stenosis. Various surgical attempts were made in the past to treat such problems, these includes pyloromyotomy, pyloroplasty, transverse and YU advancement pyloroplasty and partial or complete pylorectomy.
Keeping in view the importance of this part of stomach two surgical procedures were tried Transverse pyloroplasty and YU advancement pyloroplasty as a solution to the obstructive problems of the pylorus. Although these techniques are well established methods and have been used by the various surgeons in the past as a remedy to the stenotic problems of the pylorus but no work has been done on comparative efficacy of these two procedures concerned.
The present project was designed to study the comparative efficacy of these two techniques. Twelve healthy mongrel dogs were selected and were divided into three groups comprising four animals each.ln group A Transverse Pyloroplasty , in group B YU Advancement Pyloroplasty was performed and dogs of C group were kept as control. The efficacy of the procedures was evaluated on basis of physical examination, radiographic evaluation, and postmortem examination.
Laparotomy was performed in aseptic condition and pylorus was exteriorized in both the groups. In dogs of group A, longitudinal incision was given over the pylorus which was closed in transversed fashion. After checking any leakage at the operated site the abdomen was closed in routine manner. In group B, Y shaped incision was given at the pylorus which was converted into U. Dogs of group C were kept as control and they were not subjected to any surgical procedure.
Barium study procedure was performed in the dogs of all groups to estimate the rate of gastric emptying and post mortem was preformed to evaluate the change in diameter of the pylorus.
The results of this study clearly indicated that although both the techniques are effective in increasing the diameter and improving the gastric emptying but Y U Advancement Pyloroplasty was more useful and effective procedure.
Availability: Items available for loan: UVAS Library [Call number: 0988,T] (1).
42.
Diagnosis Of Surra In Equines By Indirection Fluorescent Anitobody
by Malik Ahsan Nadeem | Dr.Asim Aslam | Dr.Kamran | Prof.Dr.Zafar Iqbal Ch | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2007Dissertation note: Trypanosomiasis (‘surra) is the most widely distributed arthropod- born protozoan disease affecting the equines. This study was conduàted to check the efficacy of indirect fluorescent antibody test (IFAT) for the diagnosis of Surra. For this purpose 200 blood samples were collected from horses and donkeys from different areas of Gujranwala district. Thin blood smears were prepared on clean glass slides and blood samples were centrifuged to separate the serum. Serum was transferred into the vacutainers and transported to laboratory. The serum was separated by centrifugation and stored at -70°C. 200 thin blood smear slides were fixed with methanol and subjected to Giemsa stain for further microscopic examination. Then the 200 thin blood smear slides were fixed with acetone for further processing in indirect fluorescent antibody test (IFAT). The prevalence rate of 2% and 6% by using thin blood smear and indirect fluorescent antibody test (IFAT) was obtained respectively. The results helped us to determine accuracy of indirect fluorescent antibody test (IFAT) for diagnosis of Surra.
Availability: Items available for loan: UVAS Library [Call number: 0994,T] (1).
43.
Diagnosis Of Paratuberculosis (Johne,S Disease) In Cattle And Buffaloes Through Histopathological Techniques And Polymerase Chain Reaction
by Farhan Anwar Khan | Dr.Zafar Iqbal Ch | Dr.Asim Aslam | Prof .Dr.Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2008Dissertation note: Paratuberculosis, one of the infectious disease, is the emerging cause of poor health, low productivity and finally death due to single infectious agent among dairy and beef yielding animals (cattle and buffaloes) in the World. Mycobacterium avium subsp. paratuberculosis is the most common cause of bovine Johne's disease.
The study was conducted in Lahore to compare conventional methods and PCR for the diagnosis of paratuberculosis caused by M avium subspp. paratuberculosis in 300 cattle's and buffalo's tissue samples (150 of each specie), including terminal ileum and mesenteric lymph nodes. Conventional methods included Ziehi-Neelsen's (ZN) acid fast staining and histopathology. For M paratuberculosis insertion sequence IS 900, specific 626 bp fragment, were targeted. The sensitivity and specificity of PCR was found significant in comparison to Ziehl Neelsen staining and histopathology for the diagnosis of paratuberculosis in cattle and buffaloes.
Availability: Items available for loan: UVAS Library [Call number: 1011,T] (1).
44.
Seroprevalence Of Toxoplasma Gondii Infection In Camels In And Around Bahalwalpur
by Umer Naveed Ch | Dr.Kamran Ashraf | Dr.Azhar Maqbool | Prof.Dr.M.Sarwa | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2008Dissertation note: Toxoplasma gondii is an intracellular parasite, which infects humans and animals by ingestion of tissue cyst, raw or undercooked meat or oocysts from soil, vegetables, fruits, water, soil and food contaminated by cat faeces or by transmission through the placenta, milk and blood transfusion. Although toxoplasmosis found throughout the world. Seropositivity levels vary widely among different regions of the globe and according to sociocultural habits, geographic factors; climate and transmission routes and typically rise with age.
In present study, the overall prevalence of T gondil infection in camels at Bahwawlpur recorded as 10% by using Toxoplasma Latex Agglutination kit (LAT). 2 camels were found seropositive at 1:16 dilution showing residual or non specific immunity, 5 camels were found seropositive at 1:128 showing acquired or evolving immunity, whereas 3 camels were positive at antibody titer of 1:256 giving an evidence of present infection.
It was also noted that seropositivity of T gondii in camels was higher in age group from 6- 10 years, infection was higher in female camels having abortion history than male.
Availability: Items available for loan: UVAS Library [Call number: 1049,T] (1).
45.
Detection Of Mycobacterium Tuberculosis And Mycobacterium Bovis From Spum And Blood Samples Of Human Using a Duplex PCR
by Asma Nawaz | Prof.Dr.Zafar Iqbal Ch | Dr.Azhar | Dr.Muhammad Younas Rana | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2009Dissertation note: Tuberculosis is common infectious disease in the world. Mycobacterium tuberculosis is the most common cause of tuberculosis in the humans. Tuberculosis is endemic in Pakistan with about 1.5 million people infected. M.bovis is the major cause of gastrointestinal tuberculosis in humans.
The study was conducted in Lahore to compare 100 blood and 100 sputum samples from patients of active tuberculosis. The methods employed were conventional methods including Ziehl-Neelsen staining, culture on Lowenstein Jenson medium and biochemical tests. The Duplex PCR and conventional methods for diagnosis of tuberculosis caused by M.bovis and M.tuberculosis were compared on the sputum and blood samples. For M.tuberculosis and M.bovis the pncA gene and the species-specific 500-bp fragments were targeted in the Duplex PCR, respectively.
The sensitivity and specificity of Duplex PCR was found statistically significant in comparison to the conventional methods including Ziehl-Neelsen staining and culture for the differential diagnosis of tuberculosis caused by M.tuberculosis and M.bovis. Therefore Duplex PCR is a better choice of diagnostic test in the clinical setups where clinical urgencies necessitate a reliable, sensitive and specific test with the results in a short time period.
Availability: Items available for loan: UVAS Library [Call number: 1054,T] (1).
46.
Pathology Of Naturally Infected Broilers With Mycoplasma Gallisepticum And Its Diagnosis Through Pcr
by Aamir Islam | Dr.Asim Aslam | Prof.Dr.Mansur- | Prof.Dr.Zafar Iqbal Ch | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2009Dissertation note: Two hundred broiler birds (200) showing the clinical signs of respiratory signs from fifty (50) poultry farms located in and around Lahore District were analyzed for the detection of Mycoplasma gallisepticum. The tissue samples (trachea and lungs) were subjected to PCR using for Mycoplasma gallisepticum 16S rRNA gene amplification with a set of primers (MG14-F and MG13-R). Out of 200 samples, 86 were found positive with MG. These positive samples were further analyzed for histopathological changes. Lungs showed hemorrhages, congestion and massive necrosis. Lymphocytic infiltration and oedema was also observed in lungs sections. Liver showed coagulative necrosis around the central vein, congestion and infiltration of lymphocytes. Similarly, heart section revealed necrosis and degeneration in cardiac muscles. Trachea revealed the epithelial and mucosal infiltration with lymphocytes. Hypertrophy of epithelial mucosa and catarrhal exudates recorded in trachea. Sloughing of the mucosa and sub mucosa of varying degree was noted in trachea. Few birds showed no obvious changes in the organs but were positive on PCR analysis.
Availability: Items available for loan: UVAS Library [Call number: 1055,T] (1).
47.
Molecular Detection And Speciation Of The Canme Piropiasm
by Isma Nazli Bashir | Prof. Dr.Zrafar Iqbal Ch | Dr.Peter J.Irwin | Prof. Dr. Azhar Maqbool | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2008Dissertation note: An epidemiological study of babesiosis in dogs was conducted at Pet center, University of Veterinary and Animal Sciences, Lahore, for one year and information on age, sex and breed was gathered. It was found that from a total number of 6204, dogs up to two years of age were more susceptible than other age groups (2-4, 4-6 and above 6 years).The data regarding genders revealed that males were more prone to the disease than female dogs. As far as the breeds were concerned, crossbred dogs were more susceptible followed by Pointers, Alsatians, German shepherds and Bull terriors.Hot and humid months (June to September) have greater impact on the occurrence of disease. The study regarding identification of ticks revealed that Rhiphicephalus sanguinus is the predominant vector of the disease in Pakistan.
Molecular studies were conducted to characterize and identify the species responsible for canine babesiosis in Pakistan. In this regard, a nested polymerase chain reaction-Restriction fragment length polymorphism (PCR-RFLP) technique was employed on different specimens (Blood, Body tissues and Ticks). For this purpose blood samples were collected from twenty four chronically infected dogs and applied on the Flinders Technologies Associates (FTA) cards for transportation to Australia. Different body tissues (Liver, Spleen, Kidney, Intestine, Bone marrow and Pancreas) were procured after euthanizing the two dogs and DNA was extracted, for further studies. Similarly, the eighty eight ticks were also collected from the infested dogs in the 70% ethanol for transportation to Australia. A nested PCR-RFLP assay was used for the detection and differentiation of Piroplasm species on the basis of the 1 8S ribosomal RNA gene. The assay potentially amplified and identified Babesia gibsoni as the main canine piroplasm. Similar assays on the DNA extracted from body tissues and ticks revealed Babesia gibsoni as the main piroplasm. The PCR was found to have a high detection limit (equivalent to i0 dilution), when using the DNA extracted from blood applied to FTA cards, body tissues and ticks. A new technique was developed for extraction of DNA from FTA cards and tick, in this technique, instead of using the FTA specified punching machine, we used scalpel blades, and so the rest of the chemicals used are'generally and easily available. The same protocol was used for extraction of DNA from ticks, only chemicals used in different quantities with different spinning times. Both of which, resulted in cost reduction, less effort and speedy DNA extraction. The technique reported here has the potential to be standardized for routine DNA extractions from FTA cards and ticks.
Availability: Items available for loan: UVAS Library [Call number: 1061,T] (1).
48.
Effect Of Total Particulate Matter From Commercial Cigrettes On Wound Healing In Mice
by Sidra Abdil Rahman | Prof.Dr.Muhammad Ashraf | Dr.Sheryar Afzal | Prof.Dr.Zafar Iqbal Ch.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2009Dissertation note: ACigarette smoking is considered as the most important cause of death in developed countries. Wound healing in the skin is a multifarious orchestration of cellular processes and cigarette smoking may grounds for delayed wound healing. The aim of this study was to investigate, the plausible association between exposures of cigarette total particulate matter (TPM) on wound healing. The invivo mice model of wound healing was customized for determinenation of assorted events of wound healing. A total of 49 adult mice separated in seven groups and kept under standard conditions of ventilation and temperature. Four brands of commercially available cigarette were selected for the current study. All the treated animals were exposed to TPM ointment for 12 days. A highly considerable diminution in wound closure was pragmatic among all TPM treated groups from day 6 to day 8 post-wounding. Histological investigations unveiled a noteworthy impede in the outcome of re-epithelialization, dermal matrix regeneration and maturation of collagen bundles among all TPM-exposed wounds. Delayed commencement of neovascularization was pragmatic among TPM treated groups, at day 12 post wounding. Quantification was done with the help of scan probing image processing software and image processing system. Abbot curve, angular spectrum and different other parameters of 3D surface behavior of wounds revealed a very highly significant reduction in angiogenesis at day 6 and day 8 post-wounding, which points that application of TPM instigate extensive delay in trigging the progression of angiogenesis, resulting delayed onset of wound healing. These annotations validate the damaging effects of TPM on the healing of wounds and excessive use of TPM may lead to the production of chronic wounds and oral ulcers.
Availability: Items available for loan: UVAS Library [Call number: 1071,T] (1).
49.
Comparison Of Different Diagnostic Techniques For John'S Disease In Small Ruminants
by Saba Badar | Prof.Dr.Zafar Iqbal Ch | Dr. Mansur-ud-Din | Dr.Asim Aslam | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Paratuberculosis is one of the most hazardous infectious diseases, causing heavy economic losses due to poor health, low productivity and high fatality rate among domestic and wild ruminants. Mycobacterium avium subsp. paratuberculosis is the etiological agent of Bovine Johne's disease. In this study PCR were used to detect the presence of the Acid Fast Bacillus Mycobacterium paratuberculosis, in the intestinal tissues and Mesenteric Lymph Nodes of small ruminants causing Paratuberculosis. PCR was compared to HEY medium culture on the Herrold's Egg Yolk Media.
The samples were collected from Lahore Slaughter house and brought to the Molecular Pathology Laboratory at the Department of Pathology, University of Veterinary and Animal Sciences, Lahore. The study was conducted to compare PCR and the HEY medium culture for the diagnosis of paratuberculosis caused by M avium subsp. Paratuberculosis. A total of 500 tissue samples, 250 of the ileum and 250 of the mesenteric lymph nodes were collected randomly for the identification of Johne's disease. All samples were inoculated on the HEY medium prepared in the same laboratory aseptically. Followed by DNA extraction through the Kit method then run the PCR for insertion sequence IS 900, specific 626 bp fragment, were targetted in the genome of M paratuberculosis.
The results of the study showed more samples detected positive by PCR as compared to conventional culture methodology. Also they showed in the mass of 500 tissue samples that more bacilli are prone to the samples of small intestines than associated mesenteric lymph nodes. Regarding the sensitivity of the two techniques the PCR seemed more sensitive to detect the mycobacterium in the tissues than the conventional, laborious and time consuming HEY medium culture technique; though culture has been used as golden standard in this study also. When statistically analyzed results were insignificant due to small sample size.
The study will help in comparison of the two latest techniques for the diagnosis of M paratuberculosis, to check the validity of the better technique. In this study the sensitivity and specificity of PCR was checked and compared with culture on the HEY medium staining for the diagnosis of paratuberculosis in small ruminants.
Availability: Items available for loan: UVAS Library [Call number: 1077,T] (1).
50.
Immunohistochemical And Pathomorphological Studies Of Chronic Granulomatous Enteritis (John'S Disease) in Bovines
by Muhammad Shahid | Prof.Dr.Zafar Iqbal Ch | Dr.Asim Aslam | Prof.Dr.Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Paratuberculosis, a disease caused by Mycobacterium paratuberculosis is a peril for both livestock and human beings. The present project was designed to study the pathmorphological changes induced by the organism and standardize more reliable diagnostic techniques to identify the M paratuberculosis. Tissue samples from ileurn and mesenteric lymph nodes were randomly collected from 1 50 cattle and buffalo, each in present study that was conducted in Lahore.
Gross lesions were recorded on a Performa. The samples were subjected to acid fast staining of smears from pellets after density gradient centrifugation and paraffin embedded tissue sections. All the samples also subjected to polymerase chain reaction and immunohistochemistry. The smears prepared from bacterial pellets of mucosal and cortical scraping of terminal ileum and MLN were stained indicated 11.4 % small intestine and 12.7% lymph nodes of cattle's and 8.7% and 10.7% lymph nodes of buffalo's tissue samples were positive. ZN staining of paraffin embedded tissue showed 8.0 % small intestine and 10% MLN of cattle's and 6.0 % of small intestine and 8.7% MLN in buffalo's tissue samples were positive. On basis of PCR 5.4% intestinal tissue samples and 6.0% MLN of cattle were positive. 3.4% intestinal tissue samples and 07(4.7%) MLN of buffaloes were positive. In buffaloes 4.0% intestinal tissue samples and 6.0% MLN were positive by IHC. In cattle 6.7% intestinal tissue samples and 8.0% MLN tissue samples were positive by IHC. In cattle, 27/150(18.0%) animals showed lesions in both intestine and mesenteric lymph nodes while 5/32 (15.7%) animals showed lesions in lymph nodes only. Out of 27/150(18.0%) intestinal tissue samples, 20/27 (74.1%) samples showed corrugation of the intestinal mucosa while 7/27 (26%) showed diffuse thickness.
In buffalo, 24/150 (16.0%) animals showed lesion in both intestine and mesenteric lymph nodes while 2/26 (7.7%) animals showed lesion in lymph nodes only. Out of 24 intestinal tissue samples, 19/24(79.2%) with gross lesion, samples showed corrugation of the intestinal mucosa while 5/24(20.9%) showed diffuse thickness.
In histopathology 20/27 samples of cattle showed focal granulomatous lesions while 7/27(26%) samples showed sever infiltration of macrophages and lymphocytes while 28/32(87.5%) lymph nodes showed infiltration of paracortical and cortical region of the lymph nodes with macrophages ,lymphocytes and multinucleated giant cells While 4/32 (12.5%) samples showed moderate infiltration of macrophages.
In buffaloes 19/24 (12.7%) samples showed focal granulomatous lesions while 5/24 (20.9%) samples showed sever infiltration of macrophages and lymphocytes while 22/26 (84.7%) lymph nodes showed infiltration of paracortical and cortical region of the lymph nodes with macrophages ,lymphocytes and multinucleated giant cells While 4/26 (15.4%) samples showed moderate infiltration of macrophages.
The sensitivity and specificity of immunohistochemical method was found significant in comparison Ziehl-Neelsen staining and histopathology for the diagnosis of paratuberculosis in cattle and buffaloes.
Availability: Items available for loan: UVAS Library [Call number: 1078,T] (1).
