1.
Comparative Study On Ordinary Alum Precipitated And Special Alum Precipitated H.S Vaccine By Dense
by Sajjad Hussain | Dr. Ata-ur-rehman rizvi | Dr. Muhammad | Dr. Muhammad naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 1994Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0351,T] (1).
2.
Studies On Contribution Of Sheep & Goats In The Epidemiology Of Haemorrhagic Septicaemia
by Masood Anjum | Dr. Muhammad Amin Sheikh | Dr. Haji Ahmad | Dr. Muhammad Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 1994Dissertation note: A total of 500 animals (sheep 250, goat 250), of both sexes and different age groups were examined for harbouring Pasteurella multocida in their respiratory tracts. The samples from live animals were collected by sterile cotton swabs, gently passed in their nasal cavity. The slaughtered animal's laryngotracheal region were directly approached for collection of samples. Lungs showing pathological lesions were collected from fifty animals each of both species. Tryptic soya broth was used for primary isolation of organism.
Only one goat yielded Pasteurella multocida which proved highly virulent both for rabbits and mice. The isolate fermented glucose, fructose, mannose, mannitol, sorbitol and saccharose producing acid only. A negative reaction was observed for arabinose, dulcitol, maltose, lactose, raffinose and salacin. The organism was positive for catalase, oxidase, indole production, nitrate reduction and hydrogen sulphide production tests. It gave a negative reaction for methyl red, voges proskauer, urease activity and gelatin liquefaction tests. The organism was found non-motile in hanging drop preparation.
The organism was highly sensitive to ampiclox, cefazolin and velosef, moderately sensitive to cephalexin, slightly sensitive to tobramycin, cloxacillin and resistant to lincocin, erythrocin, amoxydillin, doxycillin and sulpha-methaxazol trimethoprim.
Amongst the various disinfectants tested, the organism showed maximum susceptibility to sanitizer and poulphene, moderate sensitivity to saniguard and sanitec and resistance to phenyl, sanitol and phenol.
The organism in infected faecal matter maintained its viability for 9 days in shady place, sunlight and at room temperature. At a controlled temperature of 32°C. with 75-80% humidity, the organism remained alive for 10 days.
Availability: Items available for loan: UVAS Library [Call number: 0373,T] (1).
3.
Serosurveilance Of A Disease Complex In Commercial Broilers
by Hassan Rizvi, Syed | Dr. Muhammad Naeem | Dr. Muhammad | Dr. Muhammad Sheikh Amin | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: In this study 400 samples of blood obtained from different farms situated around Jaranwala, an important broiler raising area of Punjab, were examined serologically. The Newcastle disease virus titer in most cases were low and non protective against exposure to field strain. The birds had antibodies against pathogens such as Infectious bronchitis virus and Infectious bursal disease virus to which they had not received vaccine indicating the presence of field strain of these viruses in the area. Some of the birds at these farms had antibodies against Hycoplasma galliseplicum, Mycoplasnia svnoviae and Salmonella gallinarum. Poor hygienic conditions coupled with poor ventilation may lead to the rapid dissemination of these pathogens within a community of birds and cause severe economic problems.
Availability: Items available for loan: UVAS Library [Call number: 0382,T] (1).
4.
Comparative Seroprevalence Of Bovine Viral Diarrhoea In Local And Exotic Cattle Breeds At Different Government
by Liaqat Ali Khan | Dr. Muhammad Naeem | Dr. Syed Ata-ur-Rehman Rizvi | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: The project was designed to find out the prevalence of bovine viral diarrhoea BVD virus antibodies in the sera of local and exotic cattle breeds, randomly selected from four different sources. For this purpose one thousand serum samples were collected and agar gel diffusion technique was used for the detection of antibodies against BVD.
The results of the study revealed that an overall 11.4% cattle population was having antibodies against BVD virus. The prevalence of the BVD in local breed was 14.4% while that of exotic breed was 8.4%. The prevalence rate of antibodies against BVDV was found higher in local cattle breeds as compared to exotic breeds. Various epidemiological factors were considered. Age of the animal was an important factor influencing the prevalence of disease. So regarding the age of the animal high prevalence rates were found in adult as compared to young animals. Maximum number of reactors were found in the adult age group.
The finding of the survey revealed a moderate exposure in cattle population. The occurrence of antibodies against BVD virus in apparently healthy unvaccinated animals was indicative for prevalence of the disease in the country. Therefore it is suggested that a complete survey should be carried out for this problem throughout the country and appropriate control measures for the disease.
Availability: Items available for loan: UVAS Library [Call number: 0392,T] (1).
5.
A Study On The Effect Of Organo Phosphate Insecticide (Neguvon) On Immune Response In Broiler Chickens
by Shoaib Ahmad | Dr. Muhammad Naeem | Dr. Muhammad Naeem | Dr. Shakil | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: Neguvon, an Organophosphate insecticide at a dose of 50mg or 100mg per kg of feed for varrying period of time administered to the birds, interfered with the development of serum antibodies in vaccinated and challenged birds. Neguvon had adverse effects on weights of lymphoid organs such as bursa of fabricius, spleen and thymus. It decreased the live body weights gain and dressed eviserated weights of birds. Neguvon had not beneficial effects on serum antibodies development. It resulted in higher postchallenged mortality of medicated vaccinated birds as compared to vaccinated control birds. So Neguvon is immunosuppressive insecticide when fed at subtoxic dosage levels.
Availability: Items available for loan: UVAS Library [Call number: 0400,T] (1).
6.
Titration Of Infectious Bursal Disease Virus In Different Organs Of Gumboro Infected Birds And Embryonated Hen
by Saeed Anwar, M | Dr. Muhammad Naeem | Dr. Haji Ahmad | Dr. Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: Infectious bursal disease virus (IBDV) was quantitated in different organs of the infected birds and in different parts of the experimentally inoculated embryonated hen eggs through agar gel precipitation technique (AGPT). The AGPT was standardized in the laboratory using known hyperimmune sera against IBDV antigen. The concentration of the agar, antibody and antigen are important factors for the development of precipitation line. Moreover, only gel containing noble agar in distilled water is important for diffusion of the antigen/antibody, while either of the other reagents such as sodium chloride, phenol crystal and sodium azide are presumably added to inhibit the microbial growth.
The serum from birds on 14 days post first bost showed 294.1 ACPT titers, while egg yolk from these bird on 30-45 days post first boost were having 68.6 AGPT titers. Postmortem examination showed the lesions
In liver,Kidney, spleen, intestine and bursa fabricius. It was observed that only bursa of fabricius showed detectable level (1:4 to 1:16) of IBD virus titers. Similarly, chorioaflantoic membrane of the inoculated egg showed 1:4 AGPT titers. From this study it was concluded that AGPT is a crude but highly reliable test for qualitative and quantitative study of IBDV antigen or antibodies.
Availability: Items available for loan: UVAS Library [Call number: 0458,T] (1).
7.
Immune Response Of Broilers Treated With Amprolium And Chloramphenicol
by Rakhshanda Perveen Cheema | Dr. Muhammad Akram Muneer | Dr. Haji Ahmad | Dr. Muhammad Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: The birds that received arnprolium at recommended dosage levels had higher mean body weight than the chioramphenicol medicated, cyclophosphamide treated or untreated control birds.
Arnprolium treatment did not adversely affect the weight of bursa of Fabricius, spleen, thymus and liver of birds. Chioramphenicol treatment slightly depressed the weight of bursa of Fabricius and thymus. Cyclophosphamide treatment of birds in early life resulted in bursal atrophy and slight depression of splenic weight.
As compared to Cyclophosphamide treated and non-rnedicated control birds, the sera of NDV vaccinated birds fed Amprolium had higher antibody titres on day 42. The sera of chioramphenicol treated NDV vaccinated birds had lower antibody titres as compared to non-medicated control birds on day 42. The post-challenge sera of NDV vaccinated birds fed Amprolium also had higher antibody titre as compared to NDV vaccinated cyclophosphamide and chioramphenicol treated birds. The NDV vaccinated chioramphenicol treated and NDV vaccinated cyclophosphamide treated had high post NDV challenge mortality and the NDV vaccinated birds on amproliurn medicated rations and those on non-medicated rations did not have any significant post NDV challenge mortality.
Availability: Items available for loan: UVAS Library [Call number: 0460,T] (1).
8.
Effects Of Live Mycoplasma Gallisepticum Vaccine On Broiler Chicks
by Mumtaz Baig, M | Dr. Muhammad Akram Munir | Dr. Muhammad Naeem | Dr. Shakeel | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: This study indicated that live Mycoplasma gallisepticurn vaccine when administered to the chickens vaccinated with Newcastle vaccination had immunosuppressive effects.
Live Mycoplasma gallisepticum vaccine partially interfered with the development of serum antibodies against NDV in vaccinated chickens. However, when live MG vaccine is done alone in the chickens it gives good serum antibody titres. rube injection to baby chicks on first 4 consecutive post hatching days with cyclophosphaniide resulted in lower body weights, poor FCR, destruction of the bursa of fabricius, poor antibody response of birds to vaccinations against MG and ND.
The weight gain and FCR studies. indicated that the birds which were vaccinated either against live MG or NDV had significantly higher body weights and FCR values than those vaccinated both against MG and NDV.
These studies further indicate that the birds vaccinated against live MG and ND had significantly lower serum antibody titres on day 48 than the birds which were vaccinated against either live MG or NDV.
From the results of this study it was concluded that F-strain MG vaccination effective the growth performance and feed conversion efficacy of broiler chicks. The live MG vaccination also interfered in the development of immune response (antibody titers) against Newcastle disease virus.
Availability: Items available for loan: UVAS Library [Call number: 0464,T] (1).
9.
Yolk And Serum: Detection Of Antibodies To Various Infectious Bronchitis Virus Strains Of Chickens
by Nadeem Ibrahim, M | Dr. Muhammad Akram Munir | Dr. Haji Ahmad | Dr. Muhammad Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: A total of 1318 blood samples and 290 egg samples were analyzed for the presence of antibodies to various strains of avian infectious bronchitis virus (AIBV). For this purpose broiler breeder, commercial layer, and layer breeder farms located in various geographical regions of the provinces of the Punjab and North West Frontier Province (NWFP) were visited, and flock histories in terms of production performances, growth patterns arid IB disease prevalence, were recorded) Attempts were made to sample only those farms which had experienced high morbidity, low production and mortality in the past growing or laying flocks housed at such farms. Samples from acute phase of illness and convalescent period were collected where ever possible. It was observed that there was a general trend to house and rear multiple age groups at the same time, the poultry farms were located at a very close distances from each other and were either not following any proper vaccination programmes or were not using any IBV vaccines. The seroprevalence of 1BV antibodies in unvaccinated flocks was more in areas where poultry farms were located in clusters or had developed into poultry estates In case of commercial layers, layer breeder and broiler breeder birds their misshapen and normal eggs were also collected for detection of antibodies to various strains/types of IBV. The serum and egg-yolk samples were analyzed using haernagglutination and haemagglutination-inhibition tests.This study indicated the presence of antibodies to various AIBV types such as Massachusettes-41 (M41), D-274 and D-1466. It was further observed that the breeder and commercial flocks which did not receive any killed or live IBV vaccination had significant levels of humoral and yolk antibodies indicating that various types of IBV were circulating in the poultry flocks in various areas of the Punjab and NWFP. The flocks indicating low egg production and quality; respiratory distress, and poor feed conversion ratios did suffer from the attack of some IBV strain(s) as they also had quite significant levels of humoral/yolk IBV antibodies)
Availability: Items available for loan: UVAS Library [Call number: 0477,T] (1).
10.
Role Of Maternally Derived Antibodies In Protection Against Infectious Bursal Disease Virus.
by Sameera Akhtar | Dr. Muhammad Akram Muneer | Dr. Haji Ahmed | Dr. Muhammad Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1996Dissertation note: This project was designed to study the role of maternally derived antibody in protection against IBD and efficacy of immunization with live and killed IBDV vaccines. A total of 250 day old chicks were divided into five groups i.e. groups A, B, C, D and E, each group having equal number of chicks. Group A was non-treated control for the study of the decay rate of maternal antibodies. The chickens of groups, B, C and D were vaccinated with live, killed and combination of live and killed IBDV vaccines. All the chicks were vaccinated with NDV vaccines except group E which was kept as negative control. There was no interference in the IBDV and NDV in the development of immunity.
The birds showed the presence of passive immunity, both through AGPT and IHA tests. Maternal antibody was detectable only through AGPT. The IHA indicated the presence of immunity in all the birds upto day 14th.
It was further observed that the birds having maternal Ab titres against IBDV (upto a titre of 5.27) also resisted the experimental challenged with the CVS-6).
All the vaccinated groups indicated the immune responses post vaccination. Both the AGP and IHA tests detected decline in immunity on 7th day post-vaccination and then a gradual increase in titres at 14th day. The titres were at the peak after day 28 post booster vaccination.
The results of challenge indicated that the birds having antibody titre (GMT=68.39) against IBDV resisted the IBDV challenge. Typical clinical signs of IBD were noted. The bursa was odematous and double in size. The spleen and thymus were slightly enlarged. Statistical analysis of lymphoid organ body weight ratio's of spleen, bursa and thymus indicated a significant differences in the vaccinated and control chickens.
Availability: Items available for loan: UVAS Library [Call number: 0480,T] (1).
11.
Physicochemical Factors Effecting The Survival Of Newcastle Disease Virus
by Rizwan Qayyum | Dr. Muhammad Naeem | Dr. asif Rabbani | Prof. Dr. S.A.R. Rizvi | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1997Dissertation note: For this research project, about 305 fresh fertile hen eggs were obtained from Veterinary Research Institute, Lahore. These eggs after cleaning were incubated at 37°C in automatic incubator for 11 days. At the 11th day, candling was done to confirm the fertility of eggs, either they are embryonated or not,. Eggs found dead at the time of candling were discarded. Fertile eggs 305 in number were inoculated with physically and chemically treated mesogenic strain of Newcastle disease virus which had already been treated and stored in plastic vials at -20°C. Each egg was inoculated with about 0.lnil of the treated viral sample. Four eggs were set for each of the factor for each time period. Four eggs were kept control in each factor in which viral suspension without physical or chemical treatment was inoculated.
The project was designed to study the effect of physical and chemical factors on the survival of Newcastle disease virus. The physical factors were temperature, p11 and UV light and chemical factors included five disinfectants like Formaline, Iosan, Phenol Aldekol and Bromosept (QAC). It was noted that at 56°C temperature virus lost its haemagglutinating activity after 45 minutes, but survived this temperature at 15 and 30 minutes exposure. It was observed that virus survived at pH 4 and 9 for 6, 12, 18 and 24 hrs but was killed at pH 1 and 13 for all the said time periods. After exposing virus to UV light, it was examined that Newcastle disease virus survived at UV light exposure for 45 minutes.
As far as the chemical factors were concerned, the results showed that 0.48% concentration of formalin inactivated virus in 30 minutes but not in 15 minutes. Other two concentrations i.e. 0.12% and 0.24% could not inactivate the virus. Phenol and Bromosept showed good antiviral activity against ND virus. 0.4% and 0.6% concentrations of Phenol inactivated the virus within 15 minutes but virus retained its HA activity at 0.2% phenol concentrations for 15, 30 and 45 minutes. The virus survived at 0.1% Bromosept concentration for 45 minutes and at 0.5% concentration for 15 minutes time but its haemagglutinating property was lost at 0.5% concentration in 30 minutes and at 1% concentration, the virus was killed within 15 minutes time. 0.1% concentration of Aldekol could not inactivate the virus in 15, 30 or 45 minutes. At its 0.5% concentration virus was inactivated after 45 minutes exposure but not at 15 and 30 minutes. However 1% Aldekol inactivated virus after 30 minutes but not within 15 minutes time. losan with 0.5% and 1.0% concentrations killed the mesogenic strain of Newcastle disease virus in 15, 30 and 45 minutes respectively. So the results of this study show that losan shows excellent antiviral activity against ND virus and is the best for disinfection of this virus at the farm. Bromosept (QAC) and Phenol should be the other two options for farmers to disinfect their sheds and hatcheries to minimize the chances of infection from Newcastle disease virus.
Availability: Items available for loan: UVAS Library [Call number: 0519,T] (1).
12.
A Study On The Effect Of Synthetic Pyrethroid Insecticide Talstar (Bifenthrim) On Immune Response In Broiler Chicken
by Fida Hussain | Prof. Dr. A.R. Rizvi | Dr. Muhammad Naeem | Prof. Dr. Rashid | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0520,T] (1).
13.
In Vitro Studies On The Effects Of Physico Chemical Factors On The Survival Of Mycoplasma Capri
by Abid Akbar, M | Dr. Muhammad Naeem | Dr. Muhammad | Dr. Muhammad Amin Sheikh | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1997Dissertation note: The physical factors under study were pH and temperature. It was observed that at p11 6.5, 7.5 and 8.5, although the growth of the organism took placed but the growth was best achieved at pH 6.5 with 30% absorbance measured by turbidometery. As far as the temperature is concerned, growth at different temperatures i.e. at room temperature (30°C), at incubator temperature (37°C), at refrigerator temperature (4°C) with respect to various sources of serum i.e. horse, cattle, buffalo, sheep and allontoic fluid, were tested and it was observed that at room temperature the growth was best in media containing buffalo serum 25% absorbance, respectively. At incubator temperature the growth of ycoplasnja pj was good enough with media containing cattle, buffalo and sheep serum, giving 25%, 32% and 33%, absorbance respectively and even at refrigerator temperatvye the growth of the said organism was available with media containing cattle serum, buffalo serum, sheep serum and allontoic fluid, giving 9%, 12%, 11.5% and 9% absorbance respectively.
The results of chemical factors showed that the survival and growth of Mycoplasma capri was best attained at these serum sources i.e. horse, cattle and buffalo giving 28%, 26% and 29.5% absorbance, respectively. The effect of different concentrtions of horse serum i.e. 5%, 15% and 20% on ithe growth of the organism depicted that 15% concentration level is good for its growth and survival, giving 31.5% absorbance. Various disinfectants i.e. Formaline, Phenol, Beloran-500 (Ciba), Detol (Reckitt & Colman) were tested for disinfection against the said organism revealed that 1% solution of Formaline, 1% solution of Phenol, 0.5% solution of Beloran-500 and 4% solution of Detol effectively killed the bacteria.
Availability: Items available for loan: UVAS Library [Call number: 0531,T] (1).
14.
Effect Of Myco Ad On Immune Response Of Broiler Chickens Against Newcastle Disease Virus
by Raza Younas, M | Dr. Muhammad Amin Sheikh | Dr. Muhammad Naeem | Dr. Shakil | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1998Dissertation note: The present project was aimed at knowing the ability of Mycoad in countering the ill effects on the health of broiler chicken, offered feed possessing acceptable levels of aflatoxins. The study was conducted on 140 birds, divided into two groups designated as A and B each given further sub-division into Al and A2, B1 and B2, all comprising equal number of birds. Groups Al and B1 were given plain feed and groups A2 and B2 were given medicated feed. The aflatoxin level of each feed sample was estimated prior to their use. The feed fed to group A birds had its aflatoxin level as 41.0 PPb, whereas the feed given to group II birds had 37.6 PPb of aflatoxin. The ill effects on the birds were determined on the basis of feed conversion ratio (FCR) values and immune response against ND virus.
The immune response of the birds given plain feed was poorer as compared to those winch were given Myco-ad treated feed. The GMT values of specific antibodies of the group Al birds came to be 36.8, 22.6, 8.6 respectively on 14th, 28th and 42nd day of the age of
birds, whereas group A2 birds had these values as 39.54, 29.69 and
11.64. The same was true for group B birds, where 111 birds gave
GMT values of 37.28, 26.64 and 41.92, respectively on 14th, 28th and
42nd day of the age of birds and B2 birds gave their values as 39.54,
29.69 and 11.64.
The FCR values based findings were also in support of birds given Myco-ad treated feed. The group Al birds gave their values as
1.26, 1.55, 1.77, 1.98, 242 and 2.32, respectively on 7th, 14th, 21st, 28th, 35th and 42nd day of the age of birds, whereas group A2 birds gave their values as 1.19, 1.50, 1.73, 1.96, 2.06 and 2.24. Similarly, group [11 birds showed FCR values of 1.29, 1.63, 1.73, 1.94, 2.08 and
2.40, respectively on 7th, 14th, 21st, 28th, 35th and 42nd day of their life, whereas group 132 birds gave their values as 1.17, 1.50, 1.68, 1.92, 2.03 and 2.24.
Availability: Items available for loan: UVAS Library [Call number: 0665,T] (1).
15.
Comparative Sensitivity Of Agpt, Iha & Elisa In Determing The Antibody Response Against Ibd Virus Using Intermediate Plus (Hot) Strain 228E and Bur706
by Asim, M | Dr . Muhammad Naeem | Dr . Khalid | Dr . Muhammad Akram Munir | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1999Dissertation note: The present project was aimed at determining the comparative sensitivity of AGPT, IHA and ELISA. By determining the maternal antibody titre against IBDV. The protective efficacy of vaccines 228-E, BUR-706 and Gumboral CT was also evaluated by challenging the vaccinated birds with virulent IBD virus. A total of 200 day-old birds were randomly divided into four equal groups designated A, B, C and D. Chicks of group A, B and C were vaccinated with 228-F, BUR-706 and Gumboral CT, respectively. Group B served as un-vaccinated control.
ELISA was found more sensitive than AGPT and IHA in determining maternal antibody titre. Higher antibody titres were produced by 228-F compared to BUR-706 and Gumboral CT, respectively. Maximum mean ELISA titres in group A, B, C and D on day 35 were 10,080, 8539, 7022 and 537, respectively.
Decline in maternal antibody titre was observed weekly. AGPT could detect it up to day 14, while IHA up to day 2] and ELISA up to day 35.
228-F [Intermediate Hot (Plus) strain] provided maximum protection in vaccinated birds when challenged on day 20 and day 35 post-vaccination.
Availability: Items available for loan: UVAS Library [Call number: 0675,T] (1).
16.
Immuno Prophylaxis Of Entrerotoxaemia In Sheep And Goats
by Shahzad Jawed | Dr . Muhammad Naeem | Dr . Asif | Dr . Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 1999Dissertation note: The present project was designed to study the comparative efficacy of three different adjuvant (Potassium aluminium sulphate, Aluminium hydroxide gel and mineral oil) for enhancing the immune potential of enterotoxaemia (combined) vaccine. T)ifferent standard biological media alongwith the supplementation of amino acids, minerals, plants and animal extracts and special modified formulated media were used for the production of alam precipitated vaccine, aluminium hydroxide gel adsorbed vaccine and oil adjuvant vaccine.
It was concluded that addition of various ingredients, including yeast extracts, trace elements, amino acids, plants and animal extract, and cystine hydrochloride in proper concentration, increased the level of prototoxin and toxin in culture media due to the availability of essential required nutrients. Mouse model was chosen to study the safety and potency test of all the vaccines. The potency of all three vaccines was compared. In this experiment alam precipitated vaccine proved inferior to aluminium hydroxide gel adsorbed and oil adjuvant vaccine. In case of oil based vaccine especially in sheep the IHA antibody was significant in vaccine having potency of 750 HU/ml but on the other hand vaccine have potency 250 and 500 HU/ml were proved non significant, and the day 45 was proved significant in developing the antibody titre than that of 15 and 30 days. Same the picture was observed in case of goals that oil adjuvant vaccine was significant that of aluminized and toxoid adsorbed vaccine. It was observed that the protection afforded to goats by multivalent clostridial vaccine was higher than afforded to sheep.
Availability: Items available for loan: UVAS Library [Call number: 0683,T] (1).
17.
Detection Of Toxoplasma Gondii Infectionin Butchers And Buffaloes By Polymerase Chain Reaction (PCR) and Latex Agglutination Test
by Rana Sajjad Ahmed | Prof. Dr. Zafar Iqbal Chaudhary | Prof. Dr. Muhammad Naeem Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2005Dissertation note: Toxoplasmosis, a common parasitic zoonotic infection is usually aymptomatic in immunocompetent persons although it may be present as lymphadenopathy, febrility, etc. but it is a life threatening opportunistic infection in congenitally infected patients and in immunocompromised individuals (those with AIDS, malignancy, organ transplantation, etc). Human beings become infected with T. gondii usually by ingesting oocysts in food and water contaminated with cat feces or by consuming tissue cysts in undercooked meat.
The diagnosis of toxoplasmosis is mainly based on serological tests latex agglutination test (LAT). Detection of specific DNA seems to be of clinical value in the ingestion of patients infected with toxoplasmosis.
In this study, latex agglutination test was used for the detection of the antibodies against Toxoplasma gondii and Polymerase Chain Reaction (PCR) based on the amplification of repetitive B1 gene of T. gondii. The study was based on a total of 200 samples involving 50 butchers, 50 buffalo's sera and whole blood respectively.
LAT established an overall infection of T. gondii in butchers and buffaloes as 20 % and 22 % respectively. The PCR analysis confirmed this T. gondii prevalence in butchers and buffaloes.
LAT proved to be an efficacious method for routine serological screening for antibodies to T. gondii. The costly and sophisticated PCR results in our investigation showed good correlation with the serological data of these patients showing that LAT can be used as an alternation to PCR. The results demonstrated that PCR analysis of clinical samples of patients suspective for acute toxoplasmosis including those with an acquired infection presented by lymphadenopathy can be a promising diagnostic method that enables direct detection of parasitic DNA.
Availability: Items available for loan: UVAS Library [Call number: 0861,T] (1).
