1.
Improvement In The Nutritive Value Of Fibrous Crop By-Products By Urea Treatment
by Rafaqat Ali | Dr. Nisar Ahmed Mian | Dr. Muhammad | Dr. Talat Naseer Pasha.
Material type: ; Format:
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Publisher: 1994Dissertation note: Wheat straw and rice straw are two of the largest annually harvested crop by-products in Pakistan, and are commonly fed to buffaloes as well as cattle as a staple roughage source. These coarse roughages are essentially energy feeds in which the cell wall constituents may be as high as 80 percent of the total dry matter. However, productivity in ruminants given such straw diets is limited, primarily due to energy deficiency. Low digestibility and low nitrogen content of these roughages does not adequately satisfy microbial growth requirements, which are therefore major limiting factors responsible for poor efficiency of nutrient utilization.
In the present study, two fibrous crop by-products - wheat straw and rice straw, were treated with urea (as a source of ammonia), at 50 percent moisture level with or without added urease source at two different temperatures (220C and 390C) for three different periods (14, 21 and 28 days). Digestibilities of the treated and untreated feedstuffs were determined by in sacco technique. All the samples were chemically analysed for proximate as well as cell wall constituents before and after any treatment and after incubation in the rumen.
Increasing duration of urea-treatment invariably increased the crude protein content in all the treatments. At 28 days, difference between crude protein values of samples treated with urea alone or urea along with urease source was not statistically significant. Crude fiber contents of wheat and rice straw was inversely effected both by higher temperature and longer treatment period. Both mineral matter contents and ether extract values were not significantly effected by any of the treatment while NFE values were significantly reduced. Decrease in NDF and hemicellulose values after treatment was observed. Addition of urease hastened the process, though at 28 days, the difference between samples treated with or without the addition of urease was non-significant. Treatments increased the in sacco disappearance of DM, CF, CP, NDF, ADF and hemicellulose. Addition of urease and higher temprature increased the rate of change. Difference between urea treated and urea plus urease treatmed samples was relatively more marked in case of wheat straw than in rice straw, though in both the feedstuffs, it was non-significant at the end of treatment i.e., 28 days.
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2.
Comparative Study On The Effects Of Dietary Aflatoxin And Detoxified Aflatoxin On Functions Of Liver In Commercial
by Kamran Younas Chohan | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Dr. Talat Naseer Pasha | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1997Dissertation note: The effect of dietary aflatoxin and detoxified aflatoxin was studied on postmortem lesion, total serum protein, serum bilirubin, SGOT and SGPT in commercial broiler, which were fed on broiler starter ration upto 21 days. From 21st day to 42nd day they were divided into 5 group and fed toxicated feed along with different treatment. Aflatoxins were produced on corn by moistened it at room temperature for 15 days, and the concentration of aflatoxin in corn was determined through direct ELISA (Brinton, 1987) from a commercial Laboratory. This corn were ground and appropriate amount of this was mixed into broiler finisher ration to generate 70 ppb aflatoxin B1 level.
One hundred day old broiler chicks were obtained from a commercial hatchery and fed on broiler starter ration upto 21 days. At 21st day these were randomly divided into 5 groups, having 20 birds each. Each birds in each. One group was received simple feed, 2nd group was received contaminated feed with aflatoxin B1 70 ppb, 3rd, 4th, and 5th group were received contaminated feed with 70 ppb aflatoxin and treated for detoxification with extrusion, sodium bentonite and Mycofix plus inclusion respectively.
All the birds were thoroughly observed throughout the experimental period to detect any clinical sign deviated from normal. All the birds of each group were slaughtered at the end of experiment at 42nd days their blood was taken for separation of serum and gross postmortem lesions were recorded.
The common clinical symptoms observed in chicken at 70 ppb level were dullness, depression, paleness of combs, wattles and shank. The severity of these symptoms were less in groups feed a diet contaminated with aflatoxin with detoxification with extrusion and Mycofix plus. The group fed diet containing sodium bentonite, normal combs, wattles and shanks. Birds were remained active.
The postmortem changes in the group receiving 70 ppb dietary aflatoxin showed the enlargement of liver, kidney, pancreas and spleen. Haemorrhages were present on pancreas and on intestine. The severity of lesion on these organs were less in groups fed on diet contaminated with aflatoxin with detoxification with extrusion, Mycofix plus and sodium bentonite. The group fed on sodium bentonite added contaminated diet has normal intestine.
The total serum protein in group 2 decreased at 70 ppb aflatoxin level in feed. In group 3 which was treated with extrusion serum protein level also decreased. In group which was treated with sodium bentonite protein level increased and showed much improvement. In group 5 it also increased but less than group 4. Increase was highly significant (P<0.01) at the end of experiment.
The total serum bilirubin in group 2 increased at 70 ppb aflatoxin level. Improvement was in group 3,5 and 4 which were treated with extrusion, Mycofix plus and sodium bentonite. Much improvement in group 3 whose feed was detoxified with extrusion process. Decrease was highly significant (P<0.01) at the end of experiment.
The activity of SGOT in group 2 were increased at 70 ppb aflatoxin level in feed as compared to control. The activity of SGOT in treated groups were also increased. The treatment means of SGOT differed significantly (P<0.01).
The activity of SGPT in group 2 was increased at 70 ppb aflatoxin level in feed as compared to control. The activity of SGPT is treated group were also increased. The treatment means of SGPT differed significantly (P<0.01).
It was concluded from this study that the aflatoxin contaminated feed can be detoxified with extrusion, Mycofix plus or with addition of sodium bentonite. Addition of these in the feed improved the general body condition and liver function of the chickens.
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3.
Effect Of Different Antioxidants In Rice Polishing On The Performance Of Broiler Chicks
by Aziz, M | Prof. Dr. Nisar Ahmad Mian | Dr. Talat Naseer Pasha | Prof. Dr.
Material type: ; Format:
print
Publisher: 1997Dissertation note: The oxidation of rice polishing and complete feed, can significantly decrease nutritional value. The end result is a loss in nutrient quality, impairmeI of health of birds, reduction in performance and increased production cost.
The detrimental effects of oxidation can be minimized by adding antioxidants to rice polishing. Quality antioxidants interrupt the reaction and control subsequent losses in nutrient value. Antioxidant protect the quality of rice polishing and feed as formulated by the nutritionist to achieve maximum growth and feed efficiency.
An investigation was undertaken to study the comparative value of various antioxidants to minimize (Peroxide value, free fatty acid number) the effect of oxidation in rice polishing on the performance of broiler chicks. Six experimental rations viz, A, B, C, D, E and F were prepared for the said purpose. Ration A served as negative control (Fresh rice polishing without antioxidant), Ration B served as positive control (Rancid rice polishing without antioxidant). Ration C (Feedox) 125 gm/ton of rice polishing, Ration D Santoquin 500 gm/ton of rice polishing, Ration E Oxistat 125 gm/ton of rice polishing, Ration F Butylated hydroxy toluene (BHT) @ 500 gm/ton of rice polishing.
One hundred and eighty, day old broiler chicks were randomly divided into six groups having thirty birds in each group and which were further subdivided into three replicates comprising of ten birds in each. The body weight, feed consumption and feed conversion ratio were recorded at the end of each week. Pancreas weight, dressing percentage, mortality percentage and comparative study of antioxidants were determined at the end of experiment. However, the peroxide value of rice polishing at fresh, rancid and on weekly basis was analyzed. The experiment lasts for six weeks.
The weight gain of the birds fed on various experimental rations A, B, C, D, E and F from 5-6 weeks of age were 645.0, 538.33, 655.33,709.17,663.33 and 643.0 gms respectively. Significantly more weight gain (709.17gm) was observed in the birds fed on ration D containing santoquin. Overall total weight gain of birds fed on experimental rations A to F were 1628.8, 1343.7, 1484.2, 1608.8, 1508.1 and 1471.1 gms respectively. However, the results revealed non-significant differences among weight gain of birds.
The results of feed consumption indicated that birds fed on different experimental rations A, B, C, D, E and F from 0-6 weeks of age were 3063.77, 3082.0, 3277.2, 3247.13, 3360.0 and 3223.33 gms respectively. However, apparently the birds fed on ration E containing Oxistat antixodant consumed more feed as compared to other rations.
The data on feed efficiency showed that birds fed on different experimental rations A, B, C, D, E and F from 5-6 weeks of age were 2.558, 2.756, 2.473, 2.334, 2.590 and 2.540 respectively. Significantly (P< 0.05) better feed utilization was observed in birds fed on ration D containing Santoquin as compared to chicks fed on ration E containing Oxistat and B containing rancid rice polishing, but there was no difference in the birds fed on ration E, A, F and C. However, the feed efficiency of birds from 0-6 weeks of age fed on experimental rations A to F were 1.969, 2.209, 2.122, 1.943, 2.133 and 2.191 respectively which revealed non-significant difference among the rations.
Present results revealed that weight of pancreas of birds fed on different experimental rations A, B, C, D, E and F from 0-6 weeks of age were 2.692, 3.735, 3.064, 2.89, 3.232 and 2.839 gms respectively. Significantly more weight of pancreas was observed in the birds fed o ration B containing rancid rice polishing. However, non-significant difference were noticed in the birds fed on other experimental rations.
The peroxide value in rice polishing at first and the end of 6th week of experiment of rations A, B, C, D, E and F 0.8, 61.2, 2.1, 1.4, 2.4 and 2.5, at 6th week 10.3, 80.7, 6.3, 3.3, 5.9 and 7.6 respectively.
It was concluded that Santoquin was the best antioxidant in controlling the oxidation process in rice polishing as compared to other antioxidants, i.e., Oxistat, Feedox and BHT used in the present experiment.
RECOMMENDATIONS
1. For further study various antioxidants with various levels and various sources of fat containing ingredients (rice polishing) at various levels of inclusion should be tested.
2. This type of study should be performed with fresh ingredients.
3. Different methods i.e. control of air, moisture, temp. Of storage period, light etc. should adopted to minimize the development of rancidity.
4. Storage duration of rice polishing should be at least six weeks.
5. Storage of feed should be at controlled environment.
6. Processing of rice polishing should be in such a way that lipase enzyme not become active.
7. Santoquin (Ethoxyquin) antoxidant proved to be best in controlling oxidation process in rice polishing i.e. peroxides formation than Feedox, Oxistat and BHT antioxidants.
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4.
Influence Of Varying Levels Of Protein With Constant Level Of Energy On The Performance Of Japanese Quails At Different Stages Of Growth
by Arif, M | Dr. Nisar Ahmed Mian | Dr. Mohammad | Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 1997Dissertation note: Protein level in the ration of quails is considered as an important factor for the efficient growth. But recent studies have shown that besides the protein level, the amino acid profile of feed is more important The present study was conducted to Investigate the optimum dietary protein levels by providing adequate amounts of lysine, methionine, according to the requirements on the performance of quails
Three hundred day old quails were randomly divided into five groups of 60 quails each. Then each group was further subdivided into three replicates of 20 quails each .Five rations A, B, C, D and E containing 22, 23, 24, 25 and 26% protein respectively with Constant Metaholisable energy 2900 Kcal/Kg ration were prepared.
The average total weight gain of chicks fed on experimental rations A, B, C, D and E was 133.89, 133.37, 132.49, 131.73 and 131.49 gms. espectively Apparentlyo the chicks fed on ration A containing 22 percent protein gained more weight. Non-significant difference among weight gain of chicks fed on experimental rations containing various protein levels was observed. The average total feed consumption per chick on experimental rations A, B, C, D and E was 403.04. 395.92, 392.39, 386.81 and 382.38 gms. respectively. The total feed consumption of the chicks fed on ration A containing 22 percent protein was highest. When data was subjected to statistical analysis non-significant difference was noticed among feed consumption of the chicks fed on different experimental rations. The average commulative feed eHicienc on experimental rations A, B, C, D and E was 3.01, 2.97, 2.96. 2.94 and 2.90 respectively. The chicks fed on ration E containing 26 percent protein consumed less feed per unit weight gain as compared to other experimental rations. Statistical analysis revealed non-significant difference among feed efficiency of the chicks fed on experimental rations containing different levels of protein. The averige values of dressing percentage of birds fed on rations A, B, C, D and E respectively were 70.35, 70.17, 69.90, 68.34 and 68.24 respectively The dressing percentage of chicks fed on ration A containing 22% protein was highest. Non-significant differ ence was noticed among dressing percentage on five protein levels. Results also showed that. dressing percentage of females was comparatively higher than males. The cost. per 100 grams of live weight excluding the cost of day old chicks for the rations A, B, C, D and E were Rs 2.30, 2.29, 2.33, 2.36 and 2.40.
The results of the present study indicated that Japanese quails can be reared efficiently and economically on ration containing 23% protein and 2900 Kcal/Kg M.E under local conditions with better weight gain and feed efficiency.
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5.
Effect Of Different Levels Of Fermacto On The Performance Of Broiler Chicks
by Abdul Salam Khan | Dr. Anjum Khalique | Dr. Muhammad | Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 1998Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0573,T] (1).
6.
Effectr Of Different Physical & Chemical Treatments On The Protein Quality Of Defatted Rice Polishing
by Iftikhar Ahmad Saleemi | Anjum Khalique | Dr. Talat Naseer Pasha | Muhammad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2000Dissertation note: The present study was conducted to improve the nutritional value of defatted rice polishing by treating it with certain chemicals and physical methods. 108 male broiler chicks of approximately similar weight were selected out of 220-day-old male broiler chicks priory fed on commercial diets for 7 days settlement period. Seven experimental diets viz, commercial diet; 1-Id treated DFRP; HCI treated and extruded; 1-1202 treated; 11202 treated and extruded; raw DFRP and protein free diet were incorporated into 7 major groups i.e. A, B, C, D, B, F, and 0. The groups 13 to F were having 18 chicks each. Each group was further divided into 2 sub-groups having 9 chicks each and each sub group was dispersed into three replicates having three chicks each. The group A and G having 9 chicks each and divided into three replicate of 3 chicks each. All sub-groups of groups fed on different experimental diets included differently treated rice polishing at 10% and 20% levels respectively.
Over all results showed that weight gain (282g), Feed consumed (387), FCR (1.37), Protein efficiency ratio (3.64), Protein digestibility percentage (93.5), Net protein utilization (59.5), biological value (63.6) were significantly higher than other treatments. It might be due to the fact that 1-1202 treatment proved better to eliminate anti-nutritional factors i.e. phytate, hemoglutinin etc., from DFRP and convert indigestible fiber into digestible form by breaking ligno-cellulose bond.
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7.
Comparative Effect Of Raw & Stabilized Rice Polising On The Performance Of Broiler Chicks
by Dawood Ahmad | Dr . Nisar Ahmad Mian | Dr . Muhammad | Dr . Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 1999Dissertation note: Owing to cheap and abundant availability of rice polishing in Pakistan, researchers are attempting to enhance its inclusion level in poultry ratio. It also compares well with cereal grains in chemical composition. But the prevalence of rancidity due to high fat and other toxic factors limiting its use in poultry. Hence rice polishing was subjected to extrusion cooking for stabilizing the fat and improving its quality. The extrusion cooking of rice polishing was done at National Feed Mill, Sheikhupura.
Present experiment was conducted to observe the comparative effect of Raw and stabilized rice polishing on the performance of broiler chicks. Seven experimental rations (Starter and Finisher) were prepared designated as A, B, C, D, E, F and 0. All rations were isocaloric and isonitrogenous. Starter and Finisher ration "A" was kept as control containing no rice polishing. In rations B, C, and D raw rice polishing was used at levels 20, 25 and 30% respectively while in rations E, F and 0 stabilized rice polishing was used at the same levels. Starter rations were fed for 28 days and finisher ration from 29 days to 42 days.
Two hundred and ten (210), one day old broiler chicks were randomly divided into 7-groups having thirty chicks in each groups and which were further sub-divided into three replicates comprising ten chicks in each.
Results of present study indicated that maximum weight gain was observed in birds fed on ration "E" whereas second best was observed in chicks fed on ration "B". The birds fad on rations E, F and 0 (extruded rice polishing) gained better weight than those fed on rations B, C and D (raw rice polishing) at same levels and of control ration.
Best feed utilization per unit gain was observed in chicks fed on ration "E" and it began to decline as level of rice polishing (raw and extruded) raised to 30%. Overall feed efficiency of birds fed on rations containing (extruded rice polishing) was better than those fed on ration containing raw rice polishing and at the same levels.
During the whole experimental period, 4% mortality was observed In birds fed on different rations. This was due to heat stress .e. due to high ambient temperature (June, July months). The average dressing percentage was 55.20, 56.00, 54.5, 54.40, 54.77, 56.07 and 54.34 respectively. A non-significant difference was observed regarding dressing percentage. The average pancrease weight also showed a non-significant difference among treatments. However, a slight increase in pancrease weight was observed in birds fed on rations containing raw rice polishing. This might be due to secretion of bile (Lipase Enzyme). With respect of cost per kg live weight gain, the rations containing extruded rice polishing were observed slightly economical as compared to ration containing raw rice polishing.
RECOMMENDATIONS
As extrusion processing holds great promise as a mean to improve efficiency of nutrient utilization, it is therefore recommended that the use of extruded feedstuff, especially for non-traditional, feed ingredients should be promoted in poultry ration. The level of extruded rice polishing can be raised safely upto 25%.
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8.
Influence Of Varying Levels Of Protein And Energy On The Performnace Of Japanese Quails
by Dr . Aamir Mahmood, Rana | Dr . Jaweed Ahmad Qureshi | Dr . Muhammad | Dr . Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 1998Dissertation note: The project was designed to find out the efficacy of ivermectin and Coumaphos against ectoparasites in cattle and buffaloes. Thirty buffaloes infested for ectoparasites were selected for the treatn,ent trials and divided into three groups i.e. Bi, B2 and B3 having ten animals in each group and thirty cattle infested for ectoparasites were selected for the treatment trials and also were divided into Cl, C2 and C3 groups.
The comparative acaricidal drug trials were conducted on 20 infested animals against ectoparasites of group Bi and Cl having ten animal in each were medicated with Ivermectin (Ivomec: MSD) injected once by s/c route at dose rate of 200 pg/kg body weight. Observation were made on 7th and 14th day post-medication. Skin scrapping examination was done. The overall efficacy of invermectin on 7th and 14th day in buffaloes was 40% and 80% and in cattle 50% and 90% respectively.
The comparative acaricidal drug trials were conducted on 20 infested animals for ectoparasites of B2 and C2 having ten animals in each were treated with Coumaphos (Asuntol: Bayer) at the dose rate of 30-50 gm/animal. On the 7th and 14th day post medication skin scraping examination was done. The efficacy of Coumaphos on 7th and 14th day in buffaloes was 50%, 50% and in cattle 70%, 70% respectively.
Animals were also observed for any side effect of both medicine. No side effects were observed during the period from treatment to last observation in any group i.e. groups Bi, Cl and B2, C2 groups. On last observation no spontaneous recovery was noted. In B3 and C3 in untreated and control groups.
From the results of present study it is concluded that lvermectin (Ivomec MSD) is the drug of choice for the treatment of ectoparasites in cattle and buffaloes when it is given at the dose rate of 0.2 mg/kg body weight by subcutaneous injection.
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9.
Effect Of Different Levels Of Lysine In Vegetable Protein Based Rations On The Performance Of Japanese Qualils
by Khalid Siddique | Dr . Nisar Ahmad Mian | Dr . Muhammad | Dr . Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 1998Dissertation note: Besides the protein levels, amino acid profile is considered to be more important at the time of ration formulation. The plant protein sources have been proved to be better source of protein because there is no danger of adulteration and also they have appropriate prices but at the same time most of the cereal are deficient in essential amino acids including lysine, methionine, therionine and tryptophane. under practical conditions these limitations may be rectified by judicious use of suitable protein rich ingredients and pure amino acids supplementation. The present study was planed to check the effect of different levels of lysine in vegetable protein based diets on the performance of Japanese quails (courtnix courtnix Japonica).
Three hundred quail chicks were randomly divided into five groups of 60 quails each and each group was further subdivided into 20 quails each. five experimental rations A, B, C, D, and E having lysine levels 1.10%, 1.20%, 1.30%, 1.40% and 1.50% were prepared.
The average total weight gain of chicks fed on rations A, B, C, D and E was 154.53, 148.13, 148.37, 145.73 and 139.86 gms respectively. The chicks fed on ration A having lysine level 1.10% gained more weight than all the other groups. The total feed consumption per chick on experimental rations A, B, C, D and E was 486.23, 503.66, 519.33, 521.00 and 502.60 gms respectively. The total feed consumed by chicks fed on ration D with lysine leveLi.40%was highest. When the data was subjected to statistical analysis, a non-significant difference was observed among the chicks fed on various experimental rations. The average commutative FCR of groups A, B, C, D and E was 3.26, 3.38, 3.40, 3.44 and 3.48 respectively. Best FCR was observed in chicks fed on ration A with lysine level 1.10%. The difference in FCR of ration A was also found to be more significant than all the other groups. The average values of dressing percentage of birds fed on rations A, B, C, D and E was 69.03, 69.65, 69.71, 68.29 and 68.61 gms respectively. The dressing percentage of chicks fed on ration C with lysine level 1.30% was best to all the other rations. Non- significant difference was observed in dressing percentage on five levels of lysine.
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10.
Effect Of Phytase On The Low Phosphorus Layer Diets Based On Canola Meal
by Shahid Nadeem, M | Dr . Nisar Ahmad Mian | Dr . Muhammad | Dr . Talat Naseer Pasha.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2000Dissertation note: The present study was conducted to asertain the effect of Phytase on the low phosphorus layer diets based on .Canola meal and egg production, egg weights, egg specific gravity, eggshell weights, eggshell thickness, feed consumption, feed efficiency, body weight gain, cost per dozen eggs and mortality were recorded.
Phytase enzyme is primarily responsible for the better conversion of minerals i.e. phosphorus, calcium and zinc. Phyase in tern generally results in the reduction of nutrients excreted with the faeces. It is now known to cause the release of phosphorus from phytic acid by catalysing a series of hydrolysis reactions. Phytate has been recognized as a nutrient because it contains phosphorus. It is also considered as anti-nutritional factor, which binds certain essential metals and reduces their availability for absorption from the diet and re-absorption after their secretion in digestive juices by forming insoluble compounds in the intestine. Phytase can hydrolyse the phytic acid present in canola meal. Canola meal is widely accepted and used ingredient in poultry and is a by-product of oil industry, available after extraction of oil from the seeds by solvent extraction.
Thirty weeks old 162 layers of Babcock 13300 strain were housed in cages (two birds in a cage). Birds were dived into 6 groups and offered different experimental rations having different levels of phosphorus i.e. 0.40%, 0.35%, 0.3%, 0.25%, 0.2% and 0.15% and then further each group was subdivided into 3 replicates having 9 birds in each replicate. Each replicate was reared as an experimental unit.
Phytase level was used as 400 FTU/kg of feed in each group having decreasing levels of phosphorus except control group (A). Seven days preliminary period was observed to remove the effects of previous diets. All the inanagemenial conditions like Feeding, watering, lighting, ventilation and temperature were provided according to requirements of strain.
The results of the study revealed that there was nonsignificant difference in egg weight, egg specific gravity, eggshell weights and eggshell thickness. There was a significant difference in egg production, feed efficiency, body weight gain and Feed consumption. For comparison of means of significant differed parameters, leist significant difference (LSD) test was applied. It was further revealed that there was non-significant difference between groups A (control with 0.4% Avl.P), B (with 0.3 5% Avl.P), C (with 0.3% Avl.P) in egg production and feed consumption and there was also improvement in feed efficiency and body weight gain. Economics analysis of different diets showed that phytase may be used in feeding of layers for getting eggs at low price.
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11.
Effect Of Enzyme (Kemzyme Hf) Supplementation Of Sunflower Meal On The Performance Of Japanese Quails
by Hammad-ur-Rehman | Prof. Dr. Nisar Ahmad Mian | Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2001Dissertation note: The quail has gamed an important place in the poultry farming due to its better growth rate, less space requirement that is 8 or 10 quails occupy the same space on which a broiler can be reared. They mature at an early age and the female birds are usually in full production by about 50 days of age, moreover quail meat has more protein than chicken meat. Its meat is very tasty and tender. It is fortified with nutrients required for human health and has a very low percentage of cholesterol More over quail meat has comparatively more protein percentage as compared to chicken meat and is a good source of certain B. vitamins.
Sunflower (Helianthus annuus) oil meal is a by-product obtained after the extraction of oil from the decorticated sunflower seed. Being good source of vegetable protein (34% CP) the sunflower meal can be developed as a good vegetable protein supplement for different poultry rations.
Sunflower meal is cheaper than soybean meal. It possesses high fiber content and chiorogenic acid an anti-nutritional factor, which can be reduce with the inclusion of enzymes.
Kemzyme is stabilized multi-enzyme product from Kemin Europa NV, Belgium. It contains alpha - amylase beta - glucanase, lipase, protease, cellulase and hemicellulase complex including pentosanase, xylanase, pectinase and cellubiase. The enzyme mixture is claimed to break down the non-starch polysacchardies (NSP), reduces the viscosity of gut content and improves the utilization of feed nutrients,mainly cellulase and hemicellulase complex of Kemzyme by degrading high amounts of crude fiber in the sunflower meal.
The present experiment was conducted to study the effect of enzyme (Kemzyme HF) supplementation on the nutritive value of diets containing different levels of sunflower meal on the performance of Japanese quails.
Two hundred and seventy (270) day old quail chicks of mixed sexes were purchased from local hatchery and randomly divided into six groups of 45 quails each. Then each group was further subdivided into three replicates of 15 quails each and were kept in battery cages. Six isocaloric and isonitrogenous rations were prepared using three levels of sunflower meal viz., 5, 10, 15% without enzyme (Kemzyme HF) supplementation designated as A, B & C and with enzyme (Kemzyme HF) supplementation (500 g/ton) designated as D, E & F.
The data on the weekly feed consumption and body weight recorded during the experiment. The total weight gain and feed consumption were used to calculate feed conversion ration and economics. At the end of the experiment three birds from each replicate were slaughtered to determine the dressing percentage. Proximate analysis of experimental rations were done according to AOAC (1984).
The data collected was analyzed by applying analysis of variance technique using Least Significant Difference (LSD) test to detect the difference among the treatments and means, were compared by using least significant difference test.
The results showed that the maximum weight gain (190 gm) feed consumption (515 gm), feed conversion efficiency (2.71) and dressing percentage (69%) were observed in the quails fed on ration E, containing 10% sunflower meal with enzyme supplementation. While the minimum weight gain (125 gm), lowest feed consumption (427 gm) poor feed conversion efficiency (3.41) and lowest dressing percentage (60%) were observed by the chicks fed on ration C containing 15% sunflower meal without enzyme supplementation.
Economics of different experimental rations fed to birds with and without enzyme supplementation revealed that enzyme supplementation sunflower meal based diets increased the profit margin by the reduction in feed cost per unit gain.
CONCLUSIONS
On the basis of the results of present study the following conclusions may be drawn:
1. The optimum inclusion level of sunflower meal, without dietary modification in quail rations may be below 10 percent.
2. Enzyme (Kemzyme HF) supplementation can improve the weight gain, feed consumption, feed conversion ratio and dressing percentage and was the economical.
3. Enzyme (Kernzyme HF) supplementation of quail diets can only be feasible when sunflower meal used at 10 percent level.
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12.
Studies On The Effect Of Ammoniation On The Nutritive Value Of Different Roughages In The Fattening Of Lambs
by Talat Naseer Pasha | Dr.Muhammad Yaqoob Malik | Dr . Manzoor | Dr.Saghir Ahmad Jafri.
Material type: ; Format:
print
Publisher: 1984Dissertation note: Sufficient quantity of agricultural by-products in the form of dry roughages are available in the Country for livestock feeding. The nutritive improvement of these roughages could be accomplished by proper chemical treatments. Ammoniation is one of the methods employed to improve the nutritive value of dry roughages for feeding of livestock.
A fattening trial was conducted on four groups of 64 male of Lohi lambs breed to study the effect of ammoniatin on the nutritive value of wheat straw and sugarcane pith. The roughages were treated with ammonia at 3.5 % level for 15 days and used at 22.5 % level of the four experimental rations having untreated and ammonia treated wheat straw and sugarcane pith. The experiment lasted for 84 days. Feed consumption, weight gain, feed efficiency and digestibility of the animals were recorded.
There was a significant (PLO.05) improvement in the weight gain and feed efficiency of the lambs fed ammonia treated roughages as compared with the non-treated roughages. However, there was no significant difference in the feed consumption of the animals fed different experimental rations. The digestibility trial revealed that the differences with regard to the digestibility of dry matter, crude protein,crude fibre, ether extract and nitrogen free extract between treated and untreated sugarcane pith and wheat straw were F; non-significant. But when both the roughages whether ammonia treated or untreated were compared with each other, wheat straw had significantly higher values of nutrients digestibility as compared with sugarcane pith.
The study indicated that ammoniation of wheat straw and sugarcane pith improve their nutritive value at a significant level (PLO.05) for the fattening of lambs.
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13.
Effect Of Locally Produced Phytase Enzyme On Growth Of Layer Chicks
by Farhan Ahmad | Dr. Anjum Khalique | Dr. Azhar | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2003Dissertation note: The objectives of this study were, to produce phytase on large scale and to make sure its effectiveness in utilization of phytate P in diet of growing pullets. The fungal species Aspergilus niger was used as fermentation organism, for the production of extra cellular high active phytase enzyme on defatted rice bran through solid state fermentation (SSF). Cultivation conditions for phytase production including substrate; water ratio, temperature, were optimized. Phytase production was found maximum after 96 hrs of incubation, at 40% moisture level. The developed conditions were then utilized for production of phytase on large scale basis. After production, the activity of enzyme was measured. Then this phytase was supplemented to layer grower negative control diet (NPP 0.3 1%) in different proportions i.e., 0, 1000, 1500 and 2000 tg/kg of diet to increase the bioavailability of phosphorus. Rice polishing was used as a major ingredient, as it contains high phytate contents. During experiment, feed intake, weight gain and FCR of birds were measured on weekly basis. Excreta were collected at the end of experiment to determine the effect of phytase on P availability. Also samples of toes were collected and percentage toe ash was measured.
The total feed consumption from 9-18 wk of pullets fed on diet A, B, C and D was 2919.7, 2861.9, 2913.6 and 2930.9g respectively. Weigh gain was 743.15, 718.37, 749.70 and 768.OOg respectively and FCR was 3.93, 3.99, 3.89 and 3.81 respectively.
The results showed that when low P diet (0.31% NPP) was supplemented with different levels of phytase, there was slight improvement in the performance of birds with the addition of phytase in respect of feed consumption, weight gain and FCR. Group "D", fed with diet containing 0.31% non phytate phosphorus (NPP) plus phytase (2000 igfKg of diet) showed best performance than all other groups. Group "B", fed with diet containing 0.3 1% NPP plus phytase (1000 j.tg/Kg of diet), showed poorest performance. While the performance of group "C", fed with diet containing 0.3% NPP plus phytase (l500.ig/Kg of diet) was almost similar to control group (A) fed with diet containing 0.4% NPP without phytase. The data was subjected to statistical analysis, using completely randomized design (CRD). The statistical analysis revealed non-significant difference (P> 0.05) among all treatments in respect of feed consumption, weight gain and FCR. The results were non-significant; it indicates that even lowest level of phytase (1OO0tg/Kg of diet) with 50% DCP was sufficient to maintain optimum performance of pullets. It also indicates that further investigations are required to decrease the DCP level below 50% with addition of different levels of phytase.
Phosphorus availability percentage of groups A, B, C and D was 42.67, 50.00, 54.68 and 60.67% respectively. While percentage of toe ash was 11.9, 11.2, 12.1 and 12.7% respectively. The results of P availability measured from the P intake through feed and P voided through feces showed that there was significant linear increase (P < 0.05) in percentage of P availability with increase in level of phytase. In group D (2000ig phytase 1Kg of diet) there was highest availability of P while in control group there was lowest availability of P.
In respect of toe ash there was significant difference (P < 0.05) among different experimental pullet groups. Highest toe ash percentage was in D (2000.tg phytase /Kg of diet) group while lowest in group B (l000.tg phytase /Kg of diet). Toe ash percentage of group C (1 500.tg phytase 1Kg of diet) was slightly better than group A (control). Statistically groups A and C were better then group B, but non-significant among each other. Toe ash was higher (P < 0.05) in group D than all other groups except group C.
Conclusion
- We can reduce 50% of inorganic source of phosphorus i.e. DCP with the addition of phytase, in the diet of growing pullets, with out any adverse effect on the performance of pullets.
- Other benefit of phytase supplementation is that we can improve availability of P and ultimately can reduce P in feces of birds and thus can reduce the environmental pollution.
- Further investigations are required to reduce DCP level more than 50% or even up to 0 levels with the addition of phytase.
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14.
Effect Of Autoclaving And Extrausion Of Rice Polishing On Apparent And True Metabolizable Energy In Cockerels
by Muhammad Fayyaz Ahmad | Dr. Anjum Kahliq | Dr. Ishfaq | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2004Dissertation note: The project was conducted to determine the apparent metabolizable energy (AME) and true metabolizabole energy (TME) of untreated, autoclaved and extended rice polishing in cockerels by force-feeding method. Three feedstuffs commonly used in poultry rations were assayed. Forty birds of uniform weight and size were selected out of 60 birds for experimental purpose. Each rice polishing sample was force fed to 10 cockerels for estimation of AME and TME. Ten birds were kept as control for the estimation of endogenous energy losses. The pre feeding fasting period was 24 hours and excreta were collected over a period of 24 hours. Proximate analysis for Moisture, Crude Protein, Ash, Fiber and Nitrogen free extract were carried out. The gross energy of the ingredients and the excreta were measured by using adiabatic oxygen bomb calorimeter. AME and TME were worked out; the AME values of untreated and autoclavedrice rice polishing were statistically non significant (P>0.05).
There was no affect of autoclaving on metabolizable energy contents in rice polishing. It might be due to the fact that water soluble nutrient lost during autoclaving. The values of extruded rice polishing were statistically high than untreated and autoclaved rice polishing (3255 kcal/kg). This was due to provision of heat during treatment. This high temperature may be cause of denaturing of complex carbohydrates and thus increase of soluble carbohydrates. The treatments also improve the nutritional value of rp in terms of protein, energy vitamin, minerals etc., it was noted that there was direct relationship between the crude fiber content and AME of feed stuffs. As crude fiber content of feed stuffs increased the AME values decreased and vice versa.
True metabolizable energy values were obtained by the addition of metabolic faecal and endogenous urinary energy (Fe + UE) to AME. True metabolizable energy of untreated rice polishing, autoclaved and extruded were 3532, 3516 and 3650 kcal/kg, respectively.
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15.
Effect Of Different Doses Of Bovine Somatotropin Hormone In-Ravi Buffaloes On Growth, Milk Production and Physiological Parameters
by Hafiz Faisal Majeed | Prof. Dr. Makhdoom Abdul Jabbar | Dr. Muhammad | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2007Dissertation note: The present study was conducted to see the effect of different doses of bovine sornatotropin hormone (bST) on milk production, milk composition, growth! body weight, and physiological parameters (Hemoglobin, Total protein, 1-lernatocrit values, Cholesterol, Glucose, Urea and ESR level). For this purpose 21 Nili-Ravi lactating buffaloes were involved in this experiment. They were kept at Livestock Experimental Station Bahadur Nagar Okara with similar stage of lactation were selected. They were randomly divided in to three groups A, B and C with 7 animals in each group. The group A was injected with full- recommended dose of bST hormone (250 mg /animal) with trade name of Boostin-250 S/C at an interval of 14 days, while the animals of group B were given S/C injection on alternate days with divided doses of(36 mg/animal). The group C was kept as control. The maintenance requirement of the animals was met through by available green fodder at the rate of 40-50 kg per animal per day while production requirements were met through concentrate ration at the i-ate of half of the milk production. Milk production was recorded daily and milk sarnpes weve coXeeted on weakly basis or analysis. The blood samples were collected on fortnight basis to see the effect of bovine Sornatotropin hormone on physiological parameters. The research trial continued for 6 month till the completion of lactation.
The average (rnean± SD) milk production of groups A, B and C were 7.38±1.30, 7.29±1.20 and 6.66±0.80 litters respectively. The increase in milk production was found to be 11% as compared to group C (control). The average (mean± sd) Fat, SNF and T.S percent in animals of group A were recorded 6.59±0.10, 9.13±0.28 and 15.73±0.34, respectively Where as in group B it was 6.58±0.15, 9.28±0.26 and 16.01±0.30 respectively. The averages (mcan±SD) Fat, SNF and TS percent in control group C were, 6.42±0.111, 8.83±0.47 and 15.45 ±0.245 respectively. There was improvement in Fat, SNF and TS percent of the treated group versus control group. The average (mean±SD) body weight found in subgroups A, B and group C was 528.78±50.64, 561.02±91.35 and 518.06±37.50 kgs respectively. There was slight increase was observed in treated group.
The average values of Hemoglobin, 1-Iaernatocrit, ESR, Cholesterol, Total protein, lipid glucose, and Urea in experimental animals of group A were I 1.44g/dl, 32.84%, 94.15mm/h 173.87mg/lOOrni, 10.99g/dl, 567.57 g/dl, 86.25 mg/dl, and 4.089 rng/dl and respectively. In animals of group B there were 11.96 g/dl, 32.04%, 74.70 mm/h 181.10 mg/lOOm!, 11.10 g/dl, 548.03 g/dl, 84.655 mg/dl and 3.7474 rng/dl respectively. Whereas in-group C (control) the average values of Hemoglobin, Haernatocrit, ESR Cholesterol, Total protein, lipid glucose, Urea were 12.20 g/dl, 33.11%, 83.11 mm/h 190.78, mg/lOOm!, 10.60 g/dl, 557.32 rng/dl, 84.92 rng/dl and 3.8848 g/dl, respectively. It is concluded that the bovine Somatotropin hormone had no desirable effect on physiological parameters. It is concluded that treatment of bovine sornatotropin hormone had no effect on blood composition, milk composition and physiological parameters in Nili-Ravi buffaloes. It is also concluded that dose of bSt of 250mg injected at 14 days interval is beneficial for overall performance of Nili-Ravi buffaloe. To overall bST had positive effect on economic parameters in Nili-Ravi buffaloes.
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16.
Effect Of Sodium Bentonite On Growth Performance And Feed Efficiency
by Ghayur Ahmad Khan | Prof. Dr. Makhdoom Abdul Jabbar | Prof. Dr | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2007Dissertation note: A trial was conducted to determine the effect of supplementing sodium bentonite, to find the effectiveness of sodium bentonite on the growth performance of Sahiwal male calves. Twelve animals of almost same age and weight were selected from the available stock at Animal Nutrition Centre, Rakh Dera Chahl, Lahore. These animals were divided into three groups and fed a standard ration "A" according to their nutrient requirements. Rations of group B and C were supplemented with sodium bentonite at the rate of 2.5 and 5.0 per cent while group A served as control. The trial was run for a period of 90 days with normal managemental practices. During the experimental period body weight gain and feed consumption of the individual animals was recorded. At the end of the experiment the data were analyzed statistically. The result of the experiment suggested that addition of sodium bentonite had statistically non-significant effect on the body weight gain, feed consumption, and feed conversation ratio and rate of passage of digesta through the gastro-intestinal tract. Based on the results of this trial it could be inferred that supplementation of sodium bentonite may have some beneficial effect on the performance of calves. However, use of this clay over extended period of time needs to be investigated for any adverse effect in future
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17.
Effect Of Long Term Use Of Bovine Somatotropic Hormone On Milk Production ,Production Nutrient
by Iftikhar Ahmad | Makdoom Abdul Jabbar | Dr. Talat Naseer Pasha.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2009Dissertation note: Use of bovine somatotropic hormone (bST) for increased milk production has been widely investigated in dairy cattle, whereas very little work has been done in buffaloes. To observe the effect of bST on buffalo for long term duration study was planned with the objectives to investigate the effects of long term use of bST on milk production, milk composition, reproduction, hematological and biochemical parameters in Nili-Ravi buffaloes. For this study 30 lactating Nili-Ravi buffaloes with similar milk production and stage of lactation were selected and randomly divided in to two groups A and B with 15 animals in each group. The group A (0 bST) served as control while animals in group B (+bST) were given injection of bST (250 mg Boostin-250/animal) at 14d interval. Nutritional requirements of experimental animals were met through available green fodder (45-50kg/day) supplemented with concentrate ration @ half of milk production. The milk production was significantly (P<0.05) increased by 18.04 % in treated group compared with control. The results showed that there was no significantly variations in parameters like milk composition, dry period and lactation length, calving interval in both the groups. The postpartum estrous period and service period were significantly (P< 0.05) improved which reflected positive effect of bST on reproductive parameters. However, the difference in services per conception was non-significant. Small variations were found in the prevalence of contagious and non contagious diseases in both experimental groups during the study period. The differences among body weights, hematological and biochemical parameters were also non-significant expect blood urea nitrogen (p< 0.05). The proceeds over a lactation period of 305 days was PKR. 4227.0 with the use of bST.
Second trial was conducted to study the effect of dose interval of bST in Nili-Ravi buffaloes. For the proposed study 21 Nili-Ravi lactating buffaloes with similar milk production and stage of lactation were randomly divided into three groups A, B and C with 7 animals in each group. The group A was injected with full dose of bST hormone (250 mg/animal) with trade name of Boostin-250 at an interval of 14 days, while animals in group B were given injection on alternate days with divided dose of 36 mg/animal. Group C was kept as control. Duration of study was 5 months and the animals were kept on green fodder supplemented with concentrate ration half of milk production. The concentrate ration had 17.2% CP and 72.0% TDN. The milk production increased by 18.35% and 15.27% in-group A and B compared with group C (control) but increase was non-significant (P>0.05) . Similarly data revealed that dose interval had no affect on milk contents, reproductive and hematological parameters in all the experimental groups.
In a third trial feed digestibility and efficiency for milk production was studied. For the study fourteen Nili-Ravi buffaloes at their mid lactation with almost same level of milk production were randomly divided into two groups A and B with seven animals in each group. The group A was kept as control, while group B was injected bST hormone (250 mg/animal) at an interval of 14 days and continued for 60 days. The nutritional requirements of animals in both the groups were met through TMR according to NRC recommendations. The milk production was increased by 7.0% in. treated group (B) as compared with control group (A) and the increase was statistically non-significant (P>0.05). However, the feed efficiency for milk production was significantly improved (P< 0.05) in treated group. The differences in milk composition (Fat, SNF, TS and Protein percent) body weight gain digestibility of dry matter and other nutrients in treated and control groups were found non-significant (P>0.05).
The fourth trial was conducted to determine the effect of energy on milk production and its quality under the influence of bST hormone in Nili-Ravi buffaloes. Multiparous (n12) buffaloes with mid lactation and similar level of milk yield were selected and randomly divided in to three groups i.e. A, B and C with four animals in each group. All the experimental animals were injected bST with trade name of Boostin - 250. The dose level was 250 mg per animal and injection was given at fortnightly interval during study period. The nutritional requirements of three groups animals were met through TMR with varying levels of energy (15% low and 15% above the recommendations of NRC). The milk yield was significantly higher (p<O.O5) on medium and high energy ration but the difference of milk yield was non significant (p>O.O5) between medium and low energy diets. The milk components and body weight gain were similar on all rations, while feed efficiency and nutrient intake (except ether extract) in low energy diet was significantly higher (p<O.O5) from two other rations. It may be concluded that 15% higher energy than recommended by NRC favoured milk production in Nih Ravi buffaloes when they were injected bST hormone.
Conclusion
On the over all there was consistency of results for milk production and milk composition with reference to available literature. However, some reproductive parameters including postpartum estrus and service period were significantly improved with the use of bST hormone. This effect has not been reported in the previous literature which needs to be further investigated and verified. Similarly the dose level in buffaloes needs to be further studied.
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18.
Effectis Of Supplementation Of Phytase On Growth Performance And Mineral Utilization (Ca, P & Zn) In Japanese Quail
by Muhammed Arshad Shad | Dr. Saima | Prof. Dr | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2008Dissertation note: Two third of phosphorus present in poultry diets is in bound form (Phytate P) and is predominantly not available to poultry, as non ruminant species lack sufficient endogenous phytase activity. Phytate probably compromises the utilization of protein/amino acids, energy and minerals including calcium, ferrous, zinc, magnesium, potassium & many others and can contribute to mineral deficiencies. Dietary inclusion of phytase enzyme economically generates blo-available phosphorus and reduces the Phosphorus load on the environment. Phytase is the requisite enzyme to degrade phytate and notionally phytase has the capacity to hydrolyze the phytate and release inorganic Phosphorus.
Keeping this thing in view a 28 day long feeding trial was planned. A total of 900 day old Japanese quail chicks were randomly assigned to 6 dietary treatments each having 10 replicates of 15 birds. Six corn soybean based diets (A & B) were formulated in such a way that they contained similar levels of CP and ME (24% & 2900 kcal/kg) but were different in Phosphorus & Calcium levels. Diet A was in accordance with the nutritional requirements of Japanese quail as specified in NRC (1994) whereas, diet B had lower levels of total Phosphorus and Calcium(0.20% and 0.15% less relative to diet A respectively). Five levels of phytase enzyme (250, 500, 750, 1000 FTU/kg of feed) were added to diet B to obtain diets C, D, F and F, respectively. Body weight gain, feed consumption, FCR, plasma calcium and phosphorus, zinc retention, keel /shank length, dressing %, tibia ash%, toe ash%, deficiency symptoms, mortality % and economics of all experimental rations were the parameters considered. The data thus obtained were subjected to statistical analysis using Analysis of Variance (ANOVA) Technique. The difference among the treatment means were tested by Duncans's Multiple Range (DMR) Test.
The results of the experiment revealed that body weight gain, feed consumption, FCR, plasma calcium and phosphorus, keel /shank length, dressing %, tibia ash% and toe ash% of groups E and F were significantly higher than group B, C and D, (P<0.05).The performance of group E and F was comparable with that of group A (+ve control). No significant difference was observed between these groups.
As far as deficiency symptoms and mortality is concerned, a large number in the groups B (39.30%), C (2 1.33%) and D (16.00%) was found suffering from leg weakness, swollen joints and crippled legs. A higher mortality rate was also recorded in these groups (4.67%, 3.33% and 2.67%, for groups B, C and D, respectively). Very less number of birds showed the above mentioned signs in groups A (0%), E (8.67%) and F (3.33%), similarly mortality percentage in these groups also remained considerably low (1 .33%, 2.67% and 2.00% for groups A, E and F, respectively).
Data pertaining to economics of the experiment revealed a considerably low cost of feed/kg of the group F than all the other treatment groups. The difference between group F and A was calculated as Rs. 1.89. A large amount can be saved as profit if diets are formulated with out DCP and are supplemented with phytase ensuring optimum utilization of phytate.
From the results of above experiment it is concluded that phytase supplementation ensures phytate degradation making available, the minerals and other nutrients chelated to it. Phytase supplementation not only facilitates the feed formulatin but also make possible the inclusion of ingredients which contain anti nutritional factor named, "phytate". This consequently results in considerably improved performance and ultimately higher profitability of the farm.
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19.
Studies On Levels Of Calcium And Phosphorous In Sheep And Goats Of Different Eclogical Zones Of Punjab
by Muhammad Ilyas | Prof. Dr. Makhdoom Abdul Jabbar | Prof. Dr | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2010Dissertation note: To determine the values of Calcium and Phosphorous in blood of Sheep and Goats of Centeral Mix Zone (Lahore, Kasur and Okara) and Cotton Zone (Sahiwal and Pakpattan), about 1000 blood samples were collected. In addition to blood, samples of feed, fodder, soil and water were also collected from same sites. Species under the study were sheep and goat. Sampling was done in two seasons i.e., summer and winter. For blood collection, animal were subdivided in two groups i.e., adult and young stock. In Sheep and Goats 50% samples were taken from adult animals and 50% from lambs and kids. Heparin was used as anticoagulant in blood samples. By using centrifuge machine blood plasma was separated and preserved in freezer. Feedstuff and soil samples were dried in oven and ground to 1mm particle size by using Willy mill. Water samples were given no treatment except filtration. Plasma samples were digested by using 10% TCA, whereas soil and feedstuff samples were subjected to wet digestion. After dilution all samples were run in atomic absorption spectrophotometer for estimation of Calcium and Phosphorous.
The mean plasma calcium values for Goats in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in winter season are 9.15±0.5, 8.73±0.45, 10.08±1.23, 10.12±1.3 and 8.99±0.76 respectively. The mean plasma calcium values for Goats in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in summer season are 9.18±1.1, 8.83±0.98, 9.96±1.22, 10.6±1.32 and 8.77±0.76 respectively. The mean plasma calcium values for kids in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in winter season are 9.15±1.21, 8.73±1.12, 10.1±1.67, 10.1±1.78, 8.99±1.13 respectively. The mean plasma calcium values for kids in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in summer season are 9.18±1.13, 8.83±1.11, 9.96±1.23, 10.6±1.76, 8.77±1.45 respectively.
The mean plasma calcium values for Sheep in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in winter season are 9.24±1.34, 9.26±1.22, 10.1±1.43, 9.74±1.1 and 8.4±0.98 respectively. The mean plasma calcium values for Sheep in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in summer season are 9.09±1.1, 8.80±0.99, 10.1±1.56, 10.2±1.58 and 8.93±0.99 respectively. The mean plasma calcium values for Lambs in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in winter season are 9.24±1.21, 8.88±0.98, 10.0±1.76, 10.2±1.88 and 8.71±1.34 respectively. The mean plasma calcium values for lambs in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in summer season are 8.84±0.98, 8.60±0.89, 9.98±1.13, 10.1±1.88 and 8.81±0.95 respectively.
The mean plasma Phosphorous values for Goats in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in winter season are 5.19±0.5, 6.10± 0.45, 4.84±0.34, 6.33±0.34 and 6.02±0.65 respectively. The mean plasma phosphorous values for Goats in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in summer season are 5.3 1±0.45, 6.12±0,56, 4.81±0.34, 6.29±0.75 and 6.00±0.67 respectively. The mean plasma Phosphorous values for Sheep in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in winter season are 4.95±0.45, 6.16±0.80, 5.07±0.56, 6.4±0.8 and 5.79±0.55 respectively. The mean plasma Phosphorous values for Goats in Kasur, Lahore, Okara, Pakpattan and Sahiwal districts in summer season are 5.56±0.54, 6.19±0.65, 4.72±0.43, 6.38±0.74 and 6.17±0.8 1 respectively.
The Phosphorous is present in normal range in the plasma of goats in the districts of Kasur, Okara and Pakpattan in the winter and summer seasons but the Lahore and Sahiwal districts are deficient in winter and summer seasons. The calcium is present in normal range in the plasma of sheep in the districts of Kasur, Okara and Pakpattan in the winter and summer seasons but the Lahore and Sahiwal districts are deficient in winter and summer seasons. The oat fodder, jantar, maize fodder, sorghum fodder, wheat grains, corn grains, oat grains, rice grains, wheat straw, wheat bran, rice husk and feeding plan like sorghum plus wheat straw are deficient in calcium in all the district of both zones but the grasses, barseem fodder, toria fodder, millet fodder, lucern fodder, sawank, kasni, Cotton seed cake and feeding plans like grazing, barseem plus wheat straw are normal for calcium concentration for all districts in both of the zones. All the districts are in normal range for calcium concentration in soil. Water mean calcium values were found higher in Sahiwal (3.42 ppm) district as compared to Lahore (2.65 ppm) district.
The Phosphorous is present in normal range in the plasma of goats and sheep in all the districts in the winter season and summer season.The grasses, barseem fodder, toria fodder, millet fodder, jantar, lucern, sawank, kasni maize fodder, sorghum fodder, wheat grains, corn grains, oat grains, rice grains, wheat bran cotton seed cake, grazing and feeding plans like barseem plus wheat straw, sorghum plus wheat straw are normal or marginal normal but the oat fodder, wheat straw and rice husk are deficient in phosphorous for all the districts of both zones. The Okara and Sahiwal districts are in normal range for phosphorous concentration but the Kasur, Lahore and Pakpattan are marginal deficient for phosphorous concentration. Water mean phosphorous values recorded in this study were higher in the Pakpattan (123 ppm) district and lower in Lahore (24.2 ppm) district.
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20.
Determination Of Feed Digestibility And Growth Performance Of Indian Major Carps (Catla Catla, Cirrhinus Mrigala And Labeo Rohita) Reared Under Similar Feeding Conditions
by Farzana Abbas | Prof. Dr. Naureen Aziz Qureshi | Prof. Dr. Azhar | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Artificial feed plays an important role in semi-intensive fish farming, where density of fish is high. Fish feed development is the least developed sector in aquaculture, particularly in third world countries. The formulation of nutritionally balanced and acceptable diet for fish feed is possible only if information regarding its digestibility is available. It is well known that these two parameters, nutritionally balanced diet and its digestibility, play a key role for the development of artificial feed for fish. Determination of nutrient digestibility is the first step in evaluating the potential of an ingredient for use in the diet of reared species. Information on digestibility coefficients of feed ingredients is very useful not only to enable formulation of diets that maximize fish growth by providing appropriate amounts of available nutrients but also to reduce fish wastes.
The present study was therefore, designed to find out the digestibility of nutritionally balanced diet that contains cheap and easily available ingredients. The experimental and reference diets were evaluated for comparative growth and diet utilization efficiency in three Indian major carps (Catla catla, Labeo rohita and Cirrhinus rnrigala). The highest weight gain was observed in the Cirrhinus miri gala (20.57 ± 2.8 g & 0.7 ± 0.4 cm) followed by Labeo rohita (19.17 ± 3.02g & 1.1 ± 0.4 cm) and Catla catla (19.23 ± 3.Og & 0.4 ± 0.3cm), with the experimental or test diet (TD). The lowest growth was also observed in the Catla catla (14.6 ± 3.8 g & 0.2 ± 0.1cm) with reference diet (RD). Growth, FCR and digestibility of nutrients (Protein, EE, DE) were assessed. Proximate analysis (i.e., crude protein, gross energy, dry matter and marker estimation) of feed ingredients and formulated diets was also done. The highest protein digestibility was observed in Labeo rohita (83.4±3.5%) followed by Cirrhinus mn gala (82.2 ± 4.2) and Catla catla (81.8± 4.6%) The over all digestibility's of nutrients was higher for experimental diet in three fish species i.e. crude protein (84.4 ± 2.1), dry matter (33.3 ± 8.4), crude fat (82.1± 3.1) and gross energy (61.0 ± 5.1). Nutrient digestibility of reference diet were lower (crude protein 77.2 ± 1.3, dry matter 53.0 ± 3.0, crude fat 78.7 ± 0.58 and gross energy 58.3 ± 4.5) as compared to the experimental diet.
Similarly the FCR and FCE values for experimental diet (3.1 ± 0.12 and 32.4 ± 0.69) were better than the reference diet (3.8 ± 0.06 and 25.6 ± 0.58). Among three fish species the Cirrlzinus miri gala has better FCR and FCE (3.4 ± 0.57 and 29.6 ± 5.1) followed by Cat/a catla (3.5 ± 0.42 and 29 ±.0 4.2) and Labeo rohita (3.5 ± 0.49 and 28.5 ± 4.95), respectively. FCR and FCE ratios for reference diet was the lowest in the Labeo rohita (3.9 and 25.0), whereas Cirrhinus mrigala and Cat/a cat/a showed similar ratios (3.8 0 and 26.0), respectively.
Key physico-chemical parameters viz, temperature, dissolved oxygen (DO), pH, conductivity, total dissolved solids, were regularly monitored during the study period. All the parameters showed positive significant correlation with each other except DO which showed negative significant correlation. The correlation of physico-chemical parameters with growth was negatively significant whereas positive correlation was observed between DO and growths indicating that DO have a significant effect on the growth.
The over all results showed that the experimental diet (40% protein level) has better growth and nutrient digestibility as compared to the reference diet (24.5% protein level) which resulted poorer growth and digestibility values.
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21.
Processing, Nutritional Evaluation And Utlilization Of Rumen Content For Fattening Of Nili-Ravi Male Calves
by Muhammad Waqar Khan | Prof. Dr. Arata Koga | Prof. Dr | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2010Dissertation note: The cost of feed, as percentage of total production costs, accounts for about 50-60% of ruminant feeding systems. According to estimates, Pakistan is deficient by 40 and 80 percent in forage and concentrate feeds. For economical production of balanced feed for livestock, poultry, fish and pet animal, non-conventional feed resources need to be explored to find solution to the existing gaps. For this purpose the present study was conducted to determine the nutritional profile, processing and utilization of rumen contents in buffalo feed. The study was conducted in three phases. In phase one, bovine rurnen contents were collected from the slaughter house near Bakar Mandi and were analyzed at Nutrition laboratory. UVAS, Lahore. In phase two, rumen contents were ensued with wheat straw. with and without addition of 6% molasses for a period of 90 days at Ravi Campus, Pattoki. Proportion of'rumen contents and wheat straw were 1:1 on DM basis. Wheat straw was also treated alone by reducing its DM to 44% with water and by addition of 6% molasses. In two types of silages the wheat straw was urea treated @ 4% on DM basis of wheat straw by making 25% solution. The pH of silagesi (containing rumen contents and wheat straw 50:50 on DM basis) of ruinen contents is 4.78 and of silage2 (containing rumen content, wheat straw and molasses. 47:47:6 on DM basis respectively) is 4.70. The pH of silage3 (containing rumen contents and wheat straw (urea treated), 50:46:4 respectively) is 4.92 and pH of silage4 (containing rumen contents and wheat straw (urea treated) and molasses, 47:43:4:6 respectively) is 5.1 Irespectively. The silage containing rumen contents and wheat straw showed a increase in CJ' as compare to simple rumen content and silage which were urea treated have significant (p<O.05) increase in CF. In phase three, a metabolic trial was conducted at Buffalo Reaearch Institute, Pattoki, in which 25 male buffalo calves were fed the diets having tOtal mixed ration (100%TMR) (Tc), 70%TMR with 30% silagel ('I'I), 70% 1'MR plus 30% silage2 (T2), 70%TMR with 30% silage3 ('13), and70% TMR plus 30% silage4 (T4). The data thus obtained was statistically analyzed using one way analysis of variance (ANOVA) technique. The significant differences between means were tested by Duncan's Multiple Range test. Among the treatments containing ensued rumen contents without molasses, feed efficiency was significantly (P<0.05) more efficient than those silages which were ensiled along with molasses. I lowever, the I)M intake was significantly (P<0.05) higher in control group and silage treated with noIasses and urea. When means were compared there were significant differences among all treatments for DM intake and feed efficiency. Comparatively efficient TMR (I &3) are those which formulated with those silages (silagel&3) which do not contain molasses. TMR1 is the most economical and the best efficient TMR which is formulated with silagel (containing just rumen content and wheat straw 50:50 without any treatment of urea and molasses). Even TMR formulated with silage I is more efficient than control 'l'MR and it also economical as compare to control TMR. It was concluded that rumen contents could replace 50% of conventional TMR without any adverse effects on health of buffalo calves.
Availability: Items available for loan: UVAS Library [Call number: 1214,T] (1).
22.
Nutritional Evaluation Of Locally Availabie Feed Stuffs In Different Agro- Ecological Zones Of Punjab
by Muhammad Arif | Prof. Dr. Makhdoom Abdul Jabbar | Prof. Dr. Akram | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Pakistan's economy is mainly based on agriculture and livestock production. The production per unit animal in terms of meat and milk production is low as compared to developed countries. The major reason behind this truth lies in efficient use of feed resources. In Pakistan, availability of data on indigenous feed resources in different agro-ecological zones is scanty and limited. Present study was planned to analyze the nutritional profile of locally available feed resources in different agro-ecological zones of Punjab. This study was conducted into two phases; first phase consisted of collection of samples from ten districts of Punjab. From each district, sample of fodder, dry roughages and concentrate ingredients were collected randomly from different places. The estimated total number of sample was 1020; fodder (440), dry roughages (250) and concentrate (330) samples were collected in both summer and winter seasons. The second phase of the study consisted of chemical analysis of nutritional profile of collected samples. Samples were analyzed for crude protein, ash, detergent fibres i.e., NDF, ADF, cellulose, hemi-cellulose, lignin and cell soluble contents. Data thus collected were subjected to statistical analysis using analysis of variance technique under CR Design.
In wheat straw the average CP percentage was 2.20 ± 0.25% while the value for ash content was 9.79 ± 1.97%. NDF and ADF averaged as 80.33 ± 4.43% and 52.41 ± 2.56% respectively. Higher value for protein was reported from Kasur and Sahiwal district while lowest from Sheikhupura district.
In the rice straw average CP percentage was 1.71 ± 0.24% while the value for ash content was 13.56 ± 1.91%. NDF and ADF averaged as 78.36 ± 5.40% and 54.11 ± 2.25%. Higher value for protein was reported from Sahiwal district while lowest from Lahore and Pakpatan district.
In the maize stovers average CP percentage was 2.76 ± 0.39% while the value for ash content was 7.81 ± 2.16%. NDF and ADF averaged as 75.03 ± 4.78% and 48.15 ± 3.67% respectively. Higher value for protein was reported from Kasur district while lowest from Pakpatan district.
In the sorghum stovers average CP percentage was 2.72 ± 0.15% while the value for ash content was 5.81 ± 1.16%. NDF and ADF averaged as 77.74 ± 4.60% and 49.95 ± 2.31% respectively. Higher value for protein was reported from Sahiwal district while lowest from Hafizabad district.
In the millet stovers average CP percentage was 3.03 ± 0.37% while the value for ash content was 6.45 ± 0.72%. NDF and ADF averaged as 75.80 ± 4.45% and 43.81 ± 0.94% respectively. Higher value for protein was reported from Hafizabad district while lowest from Kasur district.
In the rice husk average CP percentage was 2.99 ± 0.22% while the value for ash content was 16.37 ± 0.58%. NDF and ADF averaged as 78.90 ± 2.90% and 69.01 ± 1.17% respectively. Higher value for protein was reported from Pakpatan district while lowest from Kasur district.
In the oat fodder average CP percentage was 11.89 ± 0.74% while the value for ash content was 10.68 ± 0.57%. NDF, ADF, lignin and CSC averaged as 63.09 ± 1.88%, 32.68 ± 2.33%, 4.26 ± 0.81% and 36.91 ± 1.88% respectively. Higher value for protein was reported from Okara district while lowest from Sheikhupura district.
In the wheat fodder average CP percentage was 11.74 ± 0.51% while the value for ash content was 6.84 ± 0.78%. NDF, ADF, lignin and CSC averaged as 66.65 ± 2.70%, 36.31 ± 2.08%, 9.40 ± 1.72% and 33.35 ± 2.70% respectively. Higher value for protein was reported from Lahore district while lowest from Khushab district.
In the mustarad fodder average CP percentage was 13.42 ± 0.39% while the value for ash content was 11.57 ± 0.66%. NDF, ADF, lignin and CSC averaged as 56.19 ± 1.94%, 38.49 ± 1.98%, 12.24 ± 0.98% and 43.81 ± 1.94% respectively. Higher value for protein was reported from Muzafargarh district while lowest from Pakpatan district.
In the berseem average CP percentage was 17.32 ± 0.82% while the value for ash content was 12.94 ± 0.76%. NDF, ADF, lignin and CSC averaged as 57.37 ± 2.63%, 31.63 ± 1.90%, 10.67 ± 1.04% and 42.63 ± 2.63% respectively. Higher value for protein was reported from Pakpatan district while lowest from Hafizabad district.
In the lucern average CP percentage was 21.34 ± 0.84% while the value for ash content was 11.65 ± 1.69%. NDF, ADF, lignin and CSC averaged as 45.60 ± 2.04%, 37.73 ± 0.63%, 11.87 ±1.05% and 54.40 ± 2.04% respectively. Higher value for protein was reported from Hafizabad district while lowest from Sahiwal district
.
In the sugarcane fodder average CP percentage was 5.60 ± 0.31% while the value for ash content was 11.78 ± 0.92%. NDF, ADF, lignin and CSC averaged as 72.30 ± 3.88%, 38.20 ± 1.76%, 12.94 ± 1.13% and 27.20 ± 3.00% respectively. Higher value for protein was reported from Sheikhupura district while lowest from Lahore district.
In the grasses average CP percentage was 9.91 ± 0.48% while the value for ash content was 11.74 ± 0.94%. NDF, ADF, lignin and CSC averaged as 72.71 ± 5.20%, 36.25 ± 1.96%, 6.29 ± 0.99% and 27.29 ± 5.20% respectively. Higher value for protein was reported from Lahore district while lowest from Sheikhupura district.
In the maize fodder average CP percentage was 8.10 ± 0.75% while the value for ash content was 11.39 ± 1.58%. NDF, ADF, lignin and CSC averaged as 70.88 ± 1.94%, 32.88 ± 1.42%, 7.15 ± 1.87% and 29.12 ± 1.94% respectively. Higher value for protein was reported from Kasur district while lowest from Okara district.
In the sorghum fodder average CP percentage was 6.62 ± 0.44% while the value for ash content was 8.96 ± 1.41%. NDF, ADF, lignin and CSC averaged as 67.75 ± 2.79%, 42.11 ± 2.64%, 7.52 ± 0.96% and 32.25 ± 2.79% respectively. Higher value for protein was reported from Okara and Khushab district while lowest from Kasur district.
In the millet fodder average CP percentage was 7.76 ± 0.34% while the value for ash content was 14.80 ± 0.88%. NDF, ADF, lignin and CSC averaged as 69.90 ± 1.39%, 40.59 ± 4.54%, 9.74 ± 1.24% and 30.10 ± 1.39% respectively. Higher value for protein was reported from Sahiwal district while lowest from Khushab district.
In the corn grain average CP percentage was 9.47 ± 0.22% while the value for CSC content was 75.54 ± 1.39%. While NDF and ADF averaged as 24.46 ± 1.39% and 5.88 ± 8.47%. Higher value for protein was reported from Lahore district while lowest from Okara district.
In the wheat grain average CP percentage was 12.66 ± 0.89% while the value for CSC content was 59.75 ± 3.64%. While NDF and ADF averaged as 40.25 ± 3.64% and 4.86 ± 0.80%. Higher value for protein was reported from Lahore district while lowest from Gujranwala district.
In the Sorghum grain average CP percentage was 9.96 ± 0.42% while the value for CSC content was 49.11 ± 3.10%. While NDF and ADF averaged as 50.89 ± 3.10% and 22.06 ± 0.72%. Higher value for protein was reported from Hafizabad district while lowest from Muzafargarh district.
In the wheat bran average CP percentage was 12.53 ± 1.07% while the value for CSC content was 36.63 ± 2.24%. While NDF and ADF averaged as 63.37 ± 2.24% and 17.25 ± 2.63%. Higher value for protein was reported from Lahore district while lowest from Gujranwala district.
In the rice polish average CP percentage was 16.12 ± 0.69% while the value for CSC content was 47.58 ± 2.34%. While NDF and ADF averaged as 52.42 ± 2.34% and 18.28 ± 0.80%. Higher value for protein was reported from Hafizabad district while lowest from Kasur district.
In the cotton seed cake average CP percentage was 24.16 ± 0.36% while the value for CSC content was 40.91 ± 2.27%. While NDF and ADF averaged as 59.09 ± 2.27% and 27.65 ± 2.13%. Higher value for protein was reported from Khushab district while lowest from Sheikhupura district.
In the rape seed cake average CP percentage was 35.77 ± 0.42% while the value for CSC content was 57.42 ± 2.41%. While NDF and ADF averaged as 42.49 ± 2.59% and 24.61 ± 2.08%. Higher value for protein was reported from Sheikhupura district while lowest from Pakpatan district.
On the basis of the results of this study it is concluded that this study has generated compositional data of dry roughages, fodders and concentrates. This has lead to the preparation of dry roughages, fodders and concentrates composition tables for better feeding of livestock. The students, farmers and research workers may benefit from these tables.
It is suggested that further trials on palatability, feed intake and digestibility should be conducted to visualize practically the extent of digested nutrients in these dry roughages, fodders and concentrates.
Availability: Items available for loan: UVAS Library [Call number: 1320,T] (1).
23.
Effects Of Stair-Step Nutrition Regimen On Growth Rate, Nutrien Utilization And Pubertal Development In Nili-Ravi
by Muhammad Iqbal Anjum | Prof. Dr. Mukhdoom Abdul Jabbar | Prof. Dr | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2011Dissertation note: Under this study, effect of stair-step nutritional regimen compared to the standard NRC recommended energy levels on growth rate, nutrient utilization, some selected blood metabolites, pubertal age, conception rate and economic analysis in ili- Ravi buffalo heifers were measured. Study lasted for 18 months during the years 2008- 20 I O. Twenty-two heifers, 6-8 month old, 98.57±5.07 kg average ody weight were divided into two equal groups and randomly assigned either control or stair-step nutritional regimen (SSNR) diets. The SSNR was designed in three phase program each having 6 months duration i.e., postweaning (7 to 13 month age), repubertal (13 to 19 month age) and pubertal/breeding (19 to 25 month age). In each phase, the treatment group during step 1, was fed on low energy diet (80% ME of NRC) for 4 months followed by high energy diet (120% ME ofNRC) for 2 months in step 2. The heifers in ontrol group were fed according to NRC (200 I) requirements of Holstein Friesian heifers continuously for 6 months. For both the groups individual feeding was carried out. Daily feed intake and fortnightly fasting weights were recorded. Nutrients digestibility and N balance trials were conducted during last week of each step during each phase. Blood samples were collected at the end of each low or high energy diets for blood metabolites analysis. Oestrus detection was done with the help of a teaser bull at age of 15-16 months. Transrectal ultrasonography was done to assess uterus and ovarian structures development. Measured blood serum progesterone concentration collected every 10 days interval at 09.00-10.00 hours during 18-20 months age by ELISA using commercial kit. The age and live weight at onset of puberty was recorded when heifer tood to be mounted by the bull first time in her life. The heifers detected in oestrus were bred by natural mating at approximately 12-15 hours of the onset of oestrus activity. Heifers not returning to oestrus were examined for pregnancy diagnosis through rectal alpation of uterus at 70-90 days post breeding. Data of feed onsumption during postweaning, prepubertal and pubertallbreeding phases were used to calculate the feed cost used per kg gain between the SSNR and control heifers.
During postweaning phase, heifers fed SSNR low energy diet (2.03 Meal/kg) ained significantly (P<O.OS) lower daily weights than those fed control diet (2.SS Meal/kg), When heifers fed high energy diet (3.01 Meal/kg), daily weight gain was significantly (P<O.O 1) higher in SSNR compared to control. Average dry matter intake (DMI) was similar (P>O.OS) between the heifers of two groups. Feed conversion ratio (FCR) was poorer (P<O.OS) in SSNR heifers fed low energy diet compared to those fed control diet. But on high energy diet FCR was better (P<O.OS) in SSNR compared to control group.
During prepubertal phase, there was no difference (P>O.OS) in weight gain between the heifers fed SSNR low energy diet (1.89 Meal/kg) and control diet (2.3S Meal/kg). But on high energy diet (2.80 Meal/kg) weight gain was higher (P<O.OS) in SSNR compared to control group. Average dry matter intake (DMI) was similar (P>O.OS) between the heifers of two groups. On low energy diet there was no difference (P>O.OS) in FCR between the two groups. But on high energy diet FCR was significantly (P<O.OS) better in SSNR compared to control group. Average DMI in heifers of both groups was similar (P>O.OS). During pubertal/breeding phase, similar trend of weight gain, DMI and FCR was found in SSNR versus control group as reported in prepubertal phase.
Intake of DM, organic matter (OM) and crude protein (CP) as percent body weight were statistically non-significant (P>O.OS) differet between the SSNR versus ontrol groups during all phases. Metabolizable energy (ME) consumption was significantly P<O.OS) lower in SSNR group fed low energy diet than the heifers fed control diet. But ME consumption was significantly (P<O.O 1) increased in SSNR group fed high energy diet than control group. Similar, trend of ME consumption was observed in heifers fed SSNR (either low or high energy) and control diets during prepubertal and pubertal phases. Water to dry matter intake ratio in heifers during postweaning, prepubertal and pubertal phases were statistically similar (P>O.OS).
In all phases, apparent DM and OM digestibility did not differ (P>0.05) between the heifers fed SSNR (either low or high energy) and control diets. Neutral detergent fibre (NDF) digestibility was higher (P<0.05) when SSNR heifers fed low energy diet, but on high energy diet NDF digestibility was significantly (P<0.05) lower compared to control, respectively, during all phases with the exception of step I in the prepubertal phase and step 2 in pubertal phase where the differences were non-significant (P>0.05) between the groups. Acid detergent fibre (ADF) digestibility with SSNR low energy diet was significantly (P<0.05) higher than the heifers fed control diets during three phases. But on high energy diet, ADF digestibility was not different (P>0.05) between the two groups. Also N intake was not different (P>0.05) between the heifers fed SSNR (either low or high energy) diets and control diets, respectively, with the exception of step 2 in the postweaning phase when the control group showed a significant (P<0.05) increase in intake of N compared to the SSNR group. Faecal N as well as Urinary N losses in heifers fed SSNR (either low or high energy) versus control diets did not differ significantly (P>0.05).
All heifers have shown haematological values which are almost similar in heifers of two groups. Except total cholesterol, concentration of urea N, glucose and macro minerals in serum did not differ between the two groups.
There was no significant (P>0.05) differences in age and weight at onset of puberty and number of services per conception between the two groups. Pregnancy rate in heifers fed on SSNR diet was 50% while on control diet was 57%. Fifty percent of heifer fed SSNR and 60% of heifers fed control diet as per NRC requirement had serum progesterone concentrations> 1.0 ng/ml in two samples collected 10 days apart before reaching puberty.
The overall feed costs incurred (42660.88 vs 44509.96 Rs./animal) on SSNR heifers was significantly (P<0.05) less than the control heifers fed according to NRC recommendations from weaning to breeding age.
Availability: Items available for loan: UVAS Library [Call number: 1376,T] (1).
24.
Effect Of Multienzyme Supplementation And Acidification Of Diets On Nutrients Digestibility And Growth Performance Of Broiler
by Abdur Rahman | Dr. Saima | Prof. Dr | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Major portion of the poultry feed is composed of cereals and vegetable proteins, which cannot be fully digested and utilized by poultry due to lack of endogenous enzymes. However, it can be increased by use of exogenous enzymes. The major cost in the raising of poultry is feed. Utilization of most grains is influenced by the presence of indigestible complex carbohydrates, such as non-starch polysaccharides (NSP's) in poultry. It is possible to partially degrade these NSP's with selective exogenous enzymes acting on specific raw materials, e.g., in spite of having more gross energy in soybean meal than corn, its metabolizeable energy is less than that of corn because of -galactosides (raffinose and stachyose) that can not be digested in small intestine of broilers but these can be hydrolyzed by exogenous enzymes. Addition of organic acids in the feed has shown positive response in growth performance as they are bactericidal and reduce the incidence of Salmonellae in crop and carcass. Furthermore, organic acids are used in feed to get the favorable level of pH for the action of exogenous enzyme -galactosidase .Keeping in view the importance of supplementation of exogenous NSPs enzymes and organic acids, a 35 days trial was conducted in broiler chicks (n=240) by dividing them into eight groups and each group was having three replicates with 10 birds in each. Control group was fed NRC (1994) recommended diet (Diet A). Diet B was formulated with low metabolizeable energy (2630 Kcal/Kg). Diets C, D was formulated by adding two different levels( 0.25 gm/Kg feed and 0.5 gm/Kg feed) of NSP digesting multiezyme "Zympex" in diet B and diets E, F with different levels (0.5% and 1.5%) of citric acid in Diet B. While diets G, H was formulated by adding above mentioned different level of enzyme and citric acid in Diet B.
The weekly weight gain results showed that broilers of basal diet attained maximum weight gain which was followed by low energy diet having 500g/ton zympex, low energy diet having 500g/ton zympex plus 1.5% citric acid, low energy diet having 250g/ton zympex plus 0.5% citric acid, low energy diet with 250gm/ton zympex, low energy diet, low energy diet having 250gm/ton zympex plus 0.5% citric acid and low energy diet having 500gm/ton zympex plus 1.5% citric acid respectively. The differences of average weights of various groups when compared statistically with group A, revealed that the weights of group B, C, E, F, G and H were significantly different (p<0.05) whereas, the differences of group A and D were found non significant. While comparison of various groups with B revealed that the weight of group A, D, G and H are significantly different with B, but groups C, E and F are non significant with B. Groups B and C, C and G, B E and F, A and D, G and H are non significant among each other . On comparison of various groups with group D it was found that weights of group B, C, E, F, G and H were significantly different (p<0.05). It is evident from the above results that there is increase in weight of broilers of group D, showing non significant difference from control group A, supplemented with enzyme complex.
The results showed that maximum feed consumption was by group B followed by group C, A, D, G, E, H and F. The data of feed consumption when put to analysis of variance showed significant difference (p<0.05) of group A, B, C, D, E and G with F and H while the feed consumption of group F and H was found insignificantly different from each other. When comparison of various groups was made with feed consumption of group A, the feed consumptions of group B, C, D, E, F, G and H were found significantly different(p<0.05). While Comparison of various groups with B showed significant difference in feed consumption with all other groups.
The overall FCR of group A was found to be the best among all groups, which was followed by group D, H, G, F, C, E, and B. The analysis of variance among different experimental groups showed a significant difference (p<0.05) when groups B, C, E, F, G and H was compared with A, while D was insignificant with A. While comparing B with all groups showed significant difference with all groups. Group D and H are non significant with each other which indicate that FCR is better in D followed by H among treatment groups which is very near to control group A, showing activity of enzyme complex and acid.
The results show the values of glucose level at different time intervals, before feeding the glucose level in groups A, B, C, D, E, F, G and H showed significant results. When the data of glucose was subjected to ANOVA it revealed that group C, D, E, F, G and H were significantly different with A. Group C, D, G and H showed significant difference with B. Group B, E and F were non significant among each other. Group A showed significant different with all groups. The data obtained 1 hour after feeding when subjected to analysis of variance when compared with group A showed that groups B, D, E, F, G and H were significantly different with A. Group A, C, D, F, G and H showed significant difference with B. Group H was significantly higher among all. Data recorded after 2 hour when subjected to ANOVA revealed significant difference of group C, D, G and H with group A and B. Groups A, B, E and F are insignificant among each other. While group is significantly different with all groups. Values of data collected after 3 hours of feeding when subjected to analysis of variance showed significant difference of groups B, C, D, E, G and H with A. Groups A, C, D, G and H were significantly different with B. Group B and F were non significant among each other. Glucose level was higher in H followed by D, G, C, A, F, C and E.
The data calculated for ME when subjected to ANOVA it reveals that groups E and F are significantly different with other groups and shows non significant difference among each other. Other groups A, B, C, D, G and H are showing no significant among each other. ME in groups D and G is equal to control group A which shows the action of enzyme or acid which results in more release of energy.
Availability: Items available for loan: UVAS Library [Call number: 1440,T] (1).
25.
Effect Of Protein Supplements Of Varying Ruminal Degradability On Milk Production, Composition And Nutrients
by Illahi Bakhsh Marghazani | Prof. Dr. Makhdoom Abdul Jabbar | Prof. Dr | Prof. Dr. talat Naseer Pasha.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: The study on "Effect of protein supplements of varying ruminal degradability on milk production, composition and nutrients utilization in early lactating Sahiwal cows and Nili-Ravi buffaloes" was carried out in three phases at three different experimental locations. The in situ study of animal and vegetable protein sources was conducted at the Department of Food and Nutrition, University of Veterinary and Animals Sciences, Lahore while the feeding trials with early lactating Sahiwal cows and Nili-Ravi buffaloes were carried out Government Livestock Farm, Kalurkot, Bukkar and Livestock Experimental Station, Khushab, respectively.
Different animal (n = 6) and vegetable origin (n = 15) protein sources were subjected to ruminal protein degradability analyses using the in situ technique. All these test feeds collected from ten different locations were subjected to ruminal incubation (in triplicate) for 0, 3, 6, 12, 24 and 48 h to determine the quickly soluble fraction (a), potentially degradable fraction (b), degradation rate (c) and effective degradability at different (2, 5, 8 %) ruminal passage rates. The degradability characteristics in animal protein sources (part 1, phase 1) showed significant differences in degradation kinetics and effective degradability (ED). In crude protein (CP) degradability, the quickly soluble fraction (a) was higher (P<0.05) in fish meal, PBM and meat meal and lower (P<0.05) in blood meal, feather meal and bone and meat meal. Potentially degradable fraction (b) among test feeds was maximum (P<0.05) in bone and meat meal and PBM and minimum (P<0.05) in blood meal and feather meal. The degradation rate (c) did not differ among the test feeds. Of all the animal protein sources investigated, meat meal showed maximum CP degradability at 0.05 rumen passage rate whilst, minimum (P<0.05) ED of CP was exhibited by blood meal.
Ruminal degradability characteristics in vegetable protein sources (part-2 of phase-1) showed variation in degradation kinetics and ED of CP. The quickly soluble fraction (a) was highest (P<0.05) in sesame cake and lowest (P<0.05) in CGM 60%, coconut meal and PKC. Potentially degradable fraction (b) was maximum (P<0.05) in CGM 60%, PKC, SBM and guar meal while minimum (P<0.05) in sesame cake and CGM 30%. Protein degradation rate (c) was highest (P<0.05) in CSC while lowest (P<0.05) in coconut meal, coconut cake and CGM 60%. Effective degradability of CP at 0.05 rumen passage rate was highest in sesame cake and lowest (P<0.05) in coconut meal.
All vegetable protein sources were treated (part-3 of phase-1) with formaldehyde (1 g/100 g CP) and heat treatment (1 h at 15 lb/100 g CP) to determine their effectiveness in reducing ruminal protein degradability. Both of these treatments decreased (P<0.05) rumen degradability of the vegetable protein sources investigated. Of the formaldehyde treated test feeds, quickly soluble fraction (a) was higher (P<0.05) in sesame cake and lower (P<0.05) in CGM 60%, SBM, CGM 30%, guar meal, canola meal and coconut meal. The highest value of potentially degradable fraction (b) was recorded (P<0.05) in CSC and RSC while CGM 60% had the lowest value (P<0.05). Degradation rate (c) was highest (P<0.05) in RSM, RSC, CSC, CSM coconut cake, PKC, sesame cake, SFM and CGM 60% and lowest (P<0.05) in CGM 30%, guar meal and canola meal. Effective degradability of CP was maximum in sesame cake at all the rumen passage rates. In contrast, CGM 60% had the lowest (P<0.05) ED at all of the rumen passage rates. Among the heat treated vegetable protein sources, quickly soluble fraction (a) was highest (P<0.05) in sesame cake and lowest (P<0.05) in CGM 60% and SBM. Potentially degradable fraction (b) had the highest (P<0.05) value in almond cake, RSM, RSC, CSC and SFM while CGM 60% had the lowest value (P<0.05). Effective CP degradability of the heat treated test feeds showed that almond cake and sesame cake had the highest (P<0.05) ED whilst CGM 60% had the lowest values (P<0.05). In comparing both treatments, similar influence (P>0.05) of increasing RUP level was recorded in CGM 30%, SFM, RSM, CSM, PKC and coconut meal. Formaldehyde treatment was found more effective (P<0.05) in increasing RUP level in guar meal, canola meal, RSC, CSC, coconut cake, almond cake and sesame cake whilst heat treatment increased (P<0.05) RUP level in SBM and CGM 60% at applied rates in this study.
In phase-2, a feeding trial with early lactating Sahiwal cows was conducted to investigate the effect of protein supplements of varying ruminal degradability on milk production, composition and nutrients utilization. Twenty four early lactating Sahiwal cows were selected and randomly divided into four groups. Four iso- caloric and iso- nitrogenous diets i.e., ration A (30% RUP), ration B (40% RUP), ration C (50% RUP) and ration D (60% RUP) were fed in a completely randomized design. Dry matter and CP intakes were significantly affected by ration composition (P<0.01), whereas NDF and ADF intakes did not vary among the four treatment groups (P>0.05). DM intake was higher (P<0.05) in cows receiving rations B and A than the cows fed rations C and D. There were significant differences in DM (P<0.05), CP (P<0.001) and NDF (P<0.05) digestibility due to the ration; however, ADF digestibility did not differ (P>0.05) between the rations. DM digestibility was higher (P<0.05) on ration B than rations C and D, but similar to that for ration A. Crude protein was higher (P<0.05) on rations B and A and lower (P<0.05) on rations C and D. Daily yields of uncorrected milk and protein were highest in early lactating Sahiwal cows fed ration B and lowest when fed ration D. Daily yields of 4% FCM and milk fat were higher (P<0.05) on rations B and A and lower (P<0.05) on ration D. In milk composition, fat, protein and total solids contents were the same across all diets. Nitrogen intake was highest (P<0.01) for rations B and A and lowest for ration D and C. Nitrogen balance (g/d) and as a percentage of N intake varied; with the cows consuming ration B retaining maximum (P<0.001) N. However, N balance did not vary between rations A, C and D. Nitrogen utilization was highest (P<0.001) in cows fed ration B, but there was no difference among cows fed rations A, C and D. Live weight and body condition score in cows were unaffected by the rations. Cost of milk production was least on ration B and highest on ration D.
In phase-3 a feeding trial using early lactating Nili-Ravi buffaloes was conducted. Twenty four buffaloes were selected and randomly divided into four groups. These groups were fed four experimental diets i.e., rations A, B, C and D having 30, 40, 50 and 60% RUP proportions, respectively in a completely randomized design. Results showed no differences (P>0.05) in the intakes of DM, CP, NDF and ADF intake between the rations. Likewise, DM, CP and ADF digestibility were the same (P>0.05) in buffaloes fed rations A, B, C and D; however, NDF digestibility was higher (P<0.01) on ration C and B while lowest on rations A and D. Milk yield was highest (P<0.001) on ration C while lowest (P<0.001) on rations D and A. Buffaloes given ration C produced more (P<0.05) FCM than those receiving rations A, B and D. Daily yield of milk fat was greater (P<0.001) on ration C compared to the other three rations. Milk protein yield was highest (P<0.001) on ration C and lowest (P<0.001) on rations A and C. Diet had no effect (P>0.05) on milk fat, SNF, lactose, salts and total solids percentages; whilst milk protein percentage varied among all four diets, viz ration C>ration B>ration D>ration A. Nitrogen, intake, nitrogen balance and nitrogen utilization were similar across all the diets. Live weight and body condition score in buffaloes were unaffected by the diet fed. The cost of milk production was highest (P<0.05) with rations D and B whilst lowest (P<0.05) on ration C.
It is concluded that among animal protein sources rumen CP degradability was least in blood meal and maximum in meat meal. In vegetable protein sources, coconut meal showed least ruminal CP degradability while sesame cake recorded with highest ruminal CP degradability. Both formaldehyde and heat treatments protected protein from ruminal degradability with varied levels of effectiveness in different feeds. Production performance improved with the use of RUP sources in early lactating cows and buffaloes. Sahiwal cows showed better yield performance in diets having 40% un-degradable protein in the diet, while Nili-Ravi buffaloes showed high yield performance in diets with 50% un-degradable protein sources.
The use of latest technology and methods needs to be applied for minimizing variations involved in evaluating CP degradability of feeds through in situ procedure. Influence of RDP and RUP based rations in mid and late lactation of Sahiwal cows and Nili-Ravi buffaloes are also fertile areas of research. The studies on degradability of amino acids for compiling 'internal standards' of feed resources and production performance of lactating cows/buffaloes based on ruminal degradability of amino acids rather than protein degradability would be better approach for future studies.
Availability: Items available for loan: UVAS Library [Call number: 1470,T] (1).
26.
In-Vivo Efficacy Study Of Camel Milk In Alloxan Induced Type-1 Diabetic Rabbits
by Hina Ijaz | Dr. Muhammad Nasir | Dr. Ahmad Ali | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1473,T] (1).
27.
Effect Of Different Treatments On Tannin Contents Of Two Local Cultivars Of Sorghum Grain
by Muhammad Asad Iqbal | Dr. Saima | Dr. Athar | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
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not fiction
Publisher: 2011Dissertation note: Over the last few years, use of cereals in the poultry and livestock feed increased greatly. To meet the requirement of the feed industry, the production and proper utilization of the grains is much more important. Some grains due to presence of some anti nutritional factors limit their use in desired quantity. Similarly, their low amino acid profile and fewer digestibilities also hindered the utilization of these nutrient sources. Sorghum cereal is one of them which provide a considerable amount of protein and energy to the animal. Some other grains, sorghum contain a phenolic compound "Tannin" which is an anti nutritional component for both poultry and livestock. Tannin make a complex with protein, starch and some minerals thus reduce there availability for living beings. The present project has been planned with their objective to reduce tannin contents and increase digestibility of nutrients. The purpose of the study is the nutritional evaluation and up gradation of indigenous feed stuffs for Livestock and Poultry, so we can increase the use of sorghum grain in these industries. For this purpose two varieties of sorghum, red and white were used. Red and white sorghum varieties were treated with sodium hydroxide and formaldehyde of 0.05%, 0.1% and 0.2% solution and were kept at ambient temperature for 8, and 16 hrs. At the end of treatment, samples of each variety were tested for tannin contents and proximate analysis. In the last phase of experiment, all the treated samples of NaOH, formaldehyde and distilled water were monitored for in situ digestibility for 12, 18, 24, and 30 hrs. The data thus obtained were statistically analyzed by using completely randomized design through ANOVA Technique. The difference among treatment means were tested through Duncan's Multiple Range Test. The results of the present experiments showed that the treatment of high tannin sorghum grain with alkali or simple distilled water is the most effective method for reducing the total tannil contents in sorghum grain. It is further noticed that the alkali treatments also affect the overall nutritive value of grain and enhance the dry matter digestibility of the grain.
Availability: Items available for loan: UVAS Library [Call number: 1485,T] (1).
28.
Nutritional Characterization Of Spinach, Fenugreek And Mustard Leaves And Evaluation Of Their Glycemic Index
by Tahira Naz | Dr. Muhammad Nasir | Dr. Ali Raza | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1512,T] (1).
29.
Quality Analysis Of Drinking Water Form Various Sources Of Sahiwal City
by Farrukh Ali Ashraf | Dr.Muhammad Nasir | Prof .Dr | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1552,T] (1).
30.
Effect Of Supplemented Phytase On Phytate Phosphorus From Feed Stuffs Of Vegetable Origin In Broiler Rations.
by Hafiz Rizwan Akram | Dr. Saima | Prof. Dr | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1573,T] (1).
31.
Effect Of Inoculatied Corn Silage On Milk Production Performance Of Lactating Sahiwal Cows.
by Zahid Khan | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Makhdoom Abdul Jabbar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: Fodder and forage production in Pakistan is seasonal and this leads to erratic supply of feedstuffs to the animals. After a rich legume season, a depression period occurs, which is replaced by non-legume forages and followed again by a scarce fodder supply season. These changes in the forage supply have unfavorable effect on livestock performance. The key solution to this problem is the conservation of forages for the time, when there is feed scarcity. Keeping in view the existing availability of green fodders and silage additive products, the methods of developing alternative means to ensure availability of quality feed to animals having high nutritive value.
The Experiment was carried out at Livestock Production Research Institute, (LPRI), Bahadurnagar, Okara. Two types of corn silages were prepared, one was be untreated corn silage (Control as diet-b) and other corn silage was treated with inoculants; a commercial product "Sil-All" by ALL-TECH, (diet-a). Eighteen healthy lactating Sahiwal cows at the same milking stage were randomly distributed to two groups A and B (9 each) and were fed individually. Data was collected during the feeding experiment of 60 days duration on feed intake, daily milk production, milk composition, silage composition and body weight gain. Sahiwal cows belonging to all groups were offered concentrate according to milk production @ 1 Kg for each 3 liter of milk produced. Milk samples were analyzed for Lactose, Fat, SNF and Protein using "Milk-O-Scan" in the Dairy Laboratory of LPRI, Bahadurnagar, Okara. The feed samples were analyzed in the laboratory for Dry Matter, Crude Protein, Ether Extract, Crude Fiber, ADF, NDF and Ash contents. The data thus obtained was analyzed through Paired Sample T-test.
Daily silage intake in Sahiwal cows was 30.12±0.601 and 26.29±0.614 kg fed on inoculated silage and untreated corn silage, respectively. Inoculated silage intake was higher (P < 0.05) in Sahiwal lactating cows than cows on untreated corn silage. Milk production was higher in cows of group A fed with inoculated Silage. The results showed that Sahiwal cows fed on inoculated corn silage and untreated (control) corn silage produced 6.11±0.40 and 5.94±0.28 liters milk daily, respectively. It is also evident from the data that the cows on inoculated silage diet produced milk significantly higher (P < 0.05) than the cows under control.
Mean milk fat contents were 5.65±0.11 and 4.95±0.03 % in the milk of Sahiwal cattle fed on inoculated and untreated corn silage diets, respectively. The results showed that the cows under inoculated silage feeding produced significantly more milk fat than the cows under control. Higher (P < 0.05) milk fat contents were recorded in cows on inoculated silage than on control. Mean milk protein in the milk of Sahiwal cows fed on inoculated and untreated corn silage were 3.11±0.02 and 2.85±0.05 %, respectively. Lactose content were also higher (4.62±0.04 %) in cows on treated corn silage diet as compared to cows on control diet (4.22±0.03 %). Significantly higher (P < 0.05) solids not fat contents were recorded in cows on inoculated silage diet than the cows on untreated corn silage diet (7.72±0.14 %).
Sahiwal cows given inoculated corn silage diet had higher (329.09±5.028 kg) body weight than the cows under control (322.96±5.08 kg). A non-significant difference (P>0.05) was observed among treatment. Sahiwal cows showed a fortnightly weight gain of 01.029±0.017 and 00.712±0.010 Kg per cow fed on inoculated and untreated corn silage, respectively.
Silage pH was also improved in the diet containing inoculated silage (3.90±0.05) than the untreated silage showing a pH level at 4.08±0.35 in the untreated corn silage, showed non-significant difference (P>0.05).Dry matter contents in the inoculated corn silage had higher (30.40±0.60 %) DM % while untreated corn silage had the lowest (28.14± 0.84 %), statistically significant (P < 0.05). Mean crude protein level was 6.27±0.33 and 5.39±0.28 % in the diets having inoculated corn silage and untreated silage, respectively.
Highest value (9.17±0.75 %) of Ether Extract was recorded in inoculated corn silage diet, while untreated silage diet showed lowest (7.69± 0.24 %) value, and significant difference (P <0.05) was observed. Inoculated corn silage diet had highest (25.17±0.35 %) crude fiber level, while untreated corn silage diet showed lowest (24.51±24.51 %) crude fiber level. Inoculated silage diet had the highest (53.41±0.79 %) NDF, while untreated silage diet showed the lowest (49.34± 0.58 %) feed NDF. A significant difference (P<0.05) was observed in the inoculated diet as compared to untreated silage. The inoculated silage diet had the highest (30.23±1.00 %) ADF, while untreated silage diet had the lowest (28.26± 0.63 %) level showed a significant difference (P<0.05).The diet untreated had the highest (9.16±0.82 %) ash level while the level of Ash contents was lowest (7.69± 0.24 %) in the inoculated diet.
The results of the trial indicated significantly higher daily intake and increased milk production in Sahiwal cows was recorded when fed on inoculated silage diets. Milk constituent (Fat, Protein, Lactose and SNF) were also improved significantly in cows under inoculated corn silage diets. Sahiwal cows given inoculated corn silage diet had higher body weight than the cows under control. The composition of inoculated silage diet (DM, CF, NDF, ADF and Ash %) was also improved significantly as compared to untreated silage diet. On the basis of results presented in the study it is concluded that the use of additive or inoculant like Sill-All can be used to enhance milk production and overall performance of dairy cows.
Availability: Items available for loan: UVAS Library [Call number: 1574,T] (1).
32.
Effect Of Enzymatic Complex On Growth Performance Of Broilers At Defferent Dietary Levels Of Wheat.
by Irfan Ahmed | Sr. Saima | Dr. Athar | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2013Dissertation note: This study was conducted at environmentally controlled shed of poultry research and training centre, department of poultry production, UVAS-Ravi campus, Pattoki. The objectives of the study were to see the growth performance of broilers by use of enzymatic complex diet replacing different dietary levels of wheat with corn. Enzymatic complex (allzyme SSF) contained xylanase, beta-glucanase, pectinase, cellulase, phytase, protease and amylase was used to counteract indigestion of fibers and improved utilization. There are some evidences that the inclusion of supplemental phytase, xylanase, and the complex of enzymes might have a positive effect on broilers growth and increased the nutrients. The trial was containing 525 birds, 7 treatments and 35 replicates. Treatment 'A' is controlifor all treatments there were different levels of wheat and same quantity of enzymatic complex added to observe its effects on growth performance. Duration of trial was 35 days. 7itypes of feedsuwith unique formulation were prepared. Feed 'A' was control diet having the profile same as that of commercial feed (crude protein =21%, ME=2900 kcal/kg). The parametersiof thisiexperiment were feed intake,ibody weight gain,ifeed conversion efficiency (FCR),irelative organs weight, immune organs weight and visceral organs weight, length, mortality. The parameters were collected during the trial as well as at the end of trial. Finally,idata wasicollected, analyzedistatistically using ANOVA inicompletelyirandomized design withihelpiofia computerbbasedistatistical package (SAS). In first week the birds being offered feed 'D' were observed more feed intake and significant difference was observed among groups. In 2nd week, feed intake was decreased in birds fed enzyme supplemented feed. In first four weeks, significant difference was found between groups while last group was non-significant difference. Groups supplemented with enzyme than non-supplemented groups. In 1st, 2nd, 3rd and 4th week, control group weight gain was the best and 5th week was non-significant for all groups. The average body weight in groups supplemented with enzyme recorded was significant difference than non-supplemented with enzymes.
In case of FCR, group 'A' was observed to be the best in all groups. Analysis of variance among all groups in fifth groups found to be non-significant difference. FCR in groups supplemented with enzymes was recorded better than non-supplemented with enzymes.
Mortalities in groups A, B, C and E was the less than D, F and G. More mortality was recorded in group 'F' supplemented with 25% wheat and without enzymes. Dressing percentage was best in A and followed to B, E, F, C, G and D.
Results obtained from immune organs of bursa showed that bursa weight was found to be better in group 'G' supplemented with 25% wheat and enzyme supplemented than rest of groups in following sequence i.e. D, A, E, B, F and C. Pancreas weight of group 'B' was found to be better than result groups. Results were non-significant because weight of non-supplemented was better than supplemented with enzyme groups. While results obtained from thymus weight recorded showed that group 'G' was best supplemented with enzymes than all of other groups as followed D, A, B, C, E and F.
Results of heart weight showed that results were significant between groups except F and G groups. Best weight was observed in group 'A' control diet group. Results of liver weight showed significant difference among groups except D and E. Liver weight of control diet group was lower than other groups. Results of gizzard weight filled showed that groups supplemented with enzymes were significantly lower than groups of non-supplemented with enzyme except in groups G and F respectively. While results of empty gizzard weight of supplemented groups were found to be significantly difference increased as compared to non-supplemented with enzymes. Results of spleen weight were non-significant for all groups. Results of intestinal weight showed significant difference among the groups. Intestinal length of group 'G' found to be more than other. Intestinal length of groups supplemented with enzymes was more than non-supplemented groups. In case of intestinal weight filled and empty, non-significant difference was found among groups except in D and E.
Conclusions:
Based on finding of study, it is stated that effect of dietary supplementation of wheat and enzymatic complex in broilers diets has improved weight gain and decreased feed consumption. Similarly, dietary supplementation of wheat and enzymatic complex performed better in case of survival and less mortality was found. So it is recommended that for fibers' digestion, enzymes showed be used in diets to improve the growth performance in modern day broilers.
Availability: Items available for loan: UVAS Library [Call number: 1576,T] (1).
33.
Rheological And Microstructural Study Of Commercial Cheddar And Mozzarella Cheeses By Using Farinograph
by Saima Inayat | Prof. Dr. Muhammad Ayaz | Prof. Dr | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: A series of five experiments were conducted using Brabender Farinograph-E to study rheological properties of different brands of cheeses. This is a computerized machine having data recording capacity. It was found that Farinograph was a use full machine for preparing cheese and studying its rheology. The data recorded in the form of Farinogram showed that torque (resistance against flow of farinograph paddles) depended on fat content, temperature employed and time given to cheese formation. Also, the texture of cheese was influenced by these factors.
Sensory tests are not capable of measuring results more accurately as compared to Instrumental tests. To study cheese properties and effects of many manufacturing factors the fundamental methods will help researchers to develop cheeses with required and persistent textural and rheological properties. The instrument most frequently used all over the world for determining water absorption and mixing characteristics of wheat and rye flour in baking industry is Brabender Farinograph®. The present study was conducted by using Farinograph-E as a major tool to measure rheology of cheeses. In this study cheeses of different ages, and kinds e.g., Mozzarella, medium Cheddar, mild Cheddar, old Cheddar, extra old Cheddar, Ricotta and Parmesan were included. The parameters for operating Farinograph-E were developed and initial trials were conducted in various directions to finalize the procedure. Farinograph-E (Brabender GmbH, Duisburg, Germany) was used in this study by using its bowl W-50.
The tests were performed by cutting whole cheese bars into small pieces and shifted into air tight containers. The grated cheese was loaded with the help of spatula into Farinograph bowl. Water bath was adjusted at various temperatures like 20, 30, 40, 50 and 60°C. The temperature was continuously monitored through a temperature probe, inserted into the bowl contained cheese sample. The speed of paddles/ spindles was fixed in Newton meters (Nm) and was kept as constant for all the trials. The lid was closed after filling the bowl and clamped in order to avoid any disturbance. The test was allowed to run for specified time for 35 and 60 minutes. After completion of time durations the test was stopped automatically. The readings were recorded in the form of a graph (torque, time and temperature) of cheese dough resistance over mixing time.
Besides Farinographic studies, the results of Mozzarella and medium Cheddar Farinographic samples of (brand No name) were examined through Cryo-scanning electron microscopy and Fluorescence microscopy to study their microstructure at different stages and their relationship with quality of cheeses. The present study revealed that temperature, time and different fat percentages of different cheese brands shows significant effects on torque values. The results indicated that by increasing fat percentage the torques value decreases.
Cryo-scanning electron microscopy revealed finer details of cheeses. Shape, size and distribution of fat globules were observed through fluorescence microscopy. The changes in globule sizes and their interaction with casein matrix was also observed. Size of globule was estimated using image analysis technique. Aggregation of globules and their rupture was also observed. These changes in fat globules shape and sizes affected flowability, meltability and viscosity of cheeses and thus affected production of torques which were observed in graphs produced by Farinographs.
By studying microstructure it was obvious from micrographs that Stage 1 showed smaller fat globules in large numbers. In Stage 2 the globules became larger in size and lesser in number and like bubbles in shape, as shown in plates. At stage 3, there was no particular change from Stage 2 texture, except slight change in colour. The same changes are depicted in the shape of curve, that moved up and downwards and then upwards.
Full fats at stage 1, showed smaller fat globules those enlarged at stage 2. In stage 3, only enlarged globules were observed, and the resistance increased against paddles of farinograph and sharp increase was seen in the slope of graph. Globules retained their features at next stage and slope in graph became horizontal to x-axis after reaching maximum value.
These results suggest that size distribution of fat globules tended to impose influence on Farinographic results. Overall it is indicated that Farinograph is a suitable instrument for measuring rheology of cheeses.
Availability: Items available for loan: UVAS Library [Call number: 1578,T] (1).
34.
Influence Of Early Weaning On Growth Performance, Plasma Metabolites And Rumen Fermentation Indices In Neonatal
by Muhammad Afzal Rashid | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Makhdoom Abdul Jabbar.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Rearing of young calves is a labor intensive and costly segment of livestock production. From birth to weaning, young calf undergoes a transition from monogastric to adult ruminant. The concept of weaning from milk at an early age is based on early development of functional rumen enabling calves to utilize low quality roughages. In current study, a series of experiments were conducted to refine the more effective weaning regime for buffalo calves and use of by-products of the ethanol production industry in early weaned cattle calves.
Conventionally, buffalo calves are kept with the dam, allowed to suckle a little amount of milk along with seasonal green forages, and weaned around the age of one year. To date, limited published work was available on growth performance and economics of buffalo calves weaned from milk at an early age. Therefore, the experiment was conducted to reduce the weaning age and evaluate the growth performance of male Nili-Ravi buffalo calves. Twenty-four male buffalo calves were assigned to one of the three treatments: continuous milk feeding (CMF), limited milk feeding (LMF), and early weaning (EW). After colostrum feeding, calves were individually fed whole milk at 10% of their BW, adjusted weekly until 6 wk of age. Thereafter, milk allowance was gradually tapered to zero in CMF, LMF and EW treatments at 12, 10 and 8 wk of age, respectively. Calf starter feed was provided ad libitum from wk 2 through wk 12 and individual intakes were recorded daily. Blood sampling was carried out form wk 6 through 12, on a weekly basis. The BW and structural measurements (HG, WH, and HW) were carried out at the start of experiment and later on a weekly basis. In young buffalo calves, the regimen of weaning at 8 weeks of age was more effective. The early weaned calves showed similar growth rate to those in the CMF and LMF by consuming more calf starter and saving a substantial amount of high priced milk. On the basis of the results of this experiment, buffalo calves successfully adapted to early weaning that might help to mitigate issues like poor growth and low returns associated with traditional calf rearing practices. Furthermore, this study effectively reduced the weaning age from 1 year to 8 weeks of age. Hence, reducing weaning age did not affect the growth performance of Nili-Ravi buffalo calves by 12 weeks of age.
Early development of the rumen is the main objective of a successful early weaning program which depends upon the amount of starter intake, VFA production, and ruminal papillae development. Studies have shown that grains in starter feed can be replaced by DDGS up to 28% of DM without compromising the growth performance and rumen development. Second experiment was planned to evaluate the effects of replacing grains and soybean with DDGS and ammonia treated DDGS at 25% of DM. Study was conducted in collaboration with dairy science department SDSU (USA). Twenty one neonatal male Holstein calves were assigned to one of the three of dietary treatments: C = 0% DDGS, DDGS = 25% DDGS, CAFEX-DDGS = 25% CAFEX treated DDGS. In a 10 week experiment, calves were fed 680 g MR through 4 week, reduced to half during wk 5, and weaned at the end of wk 5. Starter intakes were conducted daily; whereas, body weights, structural measurements were conducted at the start of experiment and then on a weekly basis. Jugular blood samples were taken on a weekly basis using EDTA and NaFl coated evacuated tubes. Rumen samples were collected from a subset of 15 calves (n=5 calves/ treatment) at wk 5, 7 and 10. At the end of experiment, four calves from each treatment were also slaughtered to determine rumen morphometric measurements (PL, PW, RWT and PC). Experiment illustrated that weight gain, structural measurements, total starter intake, DMI and feed efficiency were not affected by the inclusion of DDGS and CAFEX treated DDGS at 25% of DM in starter feeds. CAFEX treatment of DDGS improved the CP contents of DDGS from 29.5% to 40%; however, inclusion of CAFEX-DDGS in starter reduced feed intake during the pre-weaning period. Whereas, overall starter intake was higher in calves fed DDGS based starter feed indicating the effect of ammonia treatment on palatability. Lower pre-weaning starter intake, slow rumen fermentation of CAFEX-DDGS resulted in lesser BHBA concentration leading to lesser development of rumen papillae growth (PL and PW). However, there was a tendency for higher weight gain in calves fed DDGS based starter due to increase in starter intake. In the light of these results it is concluded CAFEX-DDGS can be included in starter feeds at 25% of DM without affecting the growth performance. However, further research is required to evaluate the digestibility of DDGS after CAFEX treatement. Similar, growth performance indicates that CAFEX-DDGS can replace the corn and soybean meal in starter feeds.
In third experiment, microbial diversity in developing rumen and intestine of young calves fed DDGS and CAFEX treated DDGS at 25% of starter was investigated. Experiement was carried out at SDSU dairy research station (USA). Fifteen calves with n=5 per treatment, fed according to protocols described in Experiment II. Calves were sampled for rumen contents at wk 5, 7 and 10 of age; whereas, intestinal contents were collected at the time of slaughter. The DNA was extracted subjected to PCR-DGGE and dendogram was constructed using cluster analysis software. Results revealed that microbial population was highly different from each other at wk 10 indicating the effect of age and dietary treatment on rumen micro flora. Whereas, intestinal and rumen bacterial diversity at wk 5 and 7 of age was not affected by inclusion of DDGS and CAFEX-DDGS in starter feed. The changes in intestinal microflora of DDGS and CAFEX-DDGS fed calves compared with control group showed that the effect of dietary treatments on post-ruminal availability of nutrients and microbial proteins. In conclusion, rumen bacterial population changes with the advancing age and the type of ingredients used in the diet. Further, research is required to identify the effect of feeding DDGS on growth of particular bacteria like methanogen and their impact on methane production and feed efficiency.
Availability: Items available for loan: UVAS Library [Call number: 1595,T] (1).
35.
Mineral Profile In Ruminants Of Canal Lrrigated Districts Of Punjab And The Effect Of Dietary Cation Anion Diffference on Lactational Performance of Nili Ravi Buffaloes and Beetal Goats
by Umar farooq | Prof. Dr. Talat naseer pasha | Prof. Dr. Makhdoom abdul jabbar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1855,T] (1).
36.
Comparative Assessment Of Omega-3 Enriched Eggs And Supplements Against Hyperlipidemia In Human Subjects
by Amina chughtai | Dr.Muammad Nasir | Prof. Dr.Habib | Prof.Dr Talat naseer pasha.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1941,T] (1).
37.
Comparative Study Of Different Levels Of Neutral Detergent Fiber In Sahiwal Cattle And Nili-Ravi Buffalo Heifers
by Usama Tayyab | Prof. Dr. Anjum Khalique | Prof. Dr. Talat Naseer Pasha | Prof.Dr.Khalid.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1979,T] (1).
38.
Slow- Release Non-Protien Nitrogen Impact On Performance Of Weaned Crossbred Calves
by Shahzad naveed | Prof. Dr. Talat naseer pasha | Prof. Dr | Prof. Dr. Anjum khalique.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2030,T] (1).
39.
Slow-Release Non -Protien Nitrogen Impact On Performance Of Weaned Crossbred Calves
by Shahzad naveed | Prof. Dr. Talat naseer pasha | Prof. Dr.Anjum khalique.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2071,T] (1).
40.
Performance Evaluation Of Nili Ravi Buffalo Calves In Response To Wholemilk Vs Milk Replacer
by Muhammad Atif saeed | Prof. Dr. Talat naseer pasha | Dr. Muhammad Naveed ul Haque.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2072,T] (1).
41.
Productive And Physiological Performance Of Nili-Ravi Buffaloes Under Various Housing Management Practices During Summer
by Umair Younas (2002-VA-58) | Prof. Dr. Muhammad Abdullah | Dr. Jalees Ahmad Bhatti | Prof. Dr. Talat Naseer Pasha.
Material type: ; Literary form:
not fiction
Publisher: 2014Dissertation note: Back ground: Among various factors that are affecting buffalo productivity, heat stress is challenge for the dairy farmers of Pakistan since the geographical location of Pakistan is sub-tropic as it is situated 23.6 degree above the line of equator between Tropic of Cancer and Tropic of Capricorn and summer season prevail for long duration with high ambient temperature and relative humidity. Information on production potential of mature Nili-Ravi buffalo against hot-dry and hot-humid season and its adaptability to the sub-tropical conditions of central Punjab has not been documented before. In this regard, study is designed to understand the relationships of environmental stress with physiological, behavioral and production responses in Nili-Ravi buffaloes under different housing and cooling conditions.
Hypothesis: Developing and implementing housing and cooling systems to mitigate heat stress may decrease this effect but must be cost effective. To test these hypotheses, the proposed study was carried out at Buffalo Research Institute (BRI).
Methodology: Four experiments were conducted to evaluate the physiological, productive, serum biochemical and behavioral profile of mature Nili Ravi buffaloes under subtropical conditions, at BRI (Buffalo Research Institute), Pattoki. Experiments were conducted during proposed duration of March-April; May-June; July-August and September-October on various physiological and productive parameters. Lactating Nili Ravi buffaloes (n=20) were divided in to four groups with five animals in each group. Group A buffaloes were kept under roof shades
EXPERIMENT 4
157
only (control). Group B was supplemented with yeast powder under roof shade; C group buffaloes were raised under ceiling fans and group D was treated with ceiling fans and showers.
During all summer periods, fans with showers (D) showed highest milk production followed by fans only (C), then control A and B have been similar for most of times. However, in early summer (March-April), the additional cost of supplement feed and cooling strategies was higher than revenue generated from increased milk. Cost per liter of milk produced was higher and thus marginal revenue was lower than expected. Whereas, shaded group A showed comparatively less milk production which was offset by low production cost as there were no additional costs of supplementing feed or cooling strategies.
In all other periods of mid-summer (hot-dry: May-June and hot-humid: July-August) and late summer (July-August), higher milk production was noticed in group D followed by group C despite additional costs of cooling strategies. Lower cost/ liter of milk were noticed as well as high marginal revenue. During these periods, fans were noticed as more effective strategy to alleviate thermal load compare to supplemental feed as cost per liter was high and marginal revenue was less in group B. Looks like feed additive may only be applicable in early summer compare to fans group but shaded group A had best performance in terms of cost per liter and revenue. Group D was found best followed by C in terms of lower cost per liter and high marginal revenue in mid and late summer.
Statistical Analysis: The recorded data was subjected to statistical analysis by using analysis of variance technique (ANOVA) under completely randomized design (CRD). The difference of means among treatment groups were determined by using Duncan Multiple Range Test (DMRT;
SUMMARY
EXPERIMENT 4
158
Steel et al., 1997) for the interpretation of results and portraying conclusions with the help of statistical software (Statistical packages for social sciences; SPSS).
Conclusion: However, it is noted that during March and April control group buffaloes performed better economically but they had lower production and higher physiological responses. Improved performance and health of animals under fans with or without showers did not produce enough marginal income to pay for the additional costs. Shaded group A showed comparatively less milk production which was offset by low production cost as there were no additional costs of supplementing feed or cooling strategies. Therefore, small scale farmers may use shade only for their buffaloes during early summer only but progressive farmers better go for shower and fans strategy since early summer as this approach works best throughout other periods of summer season. Since, the group D was found best followed by C in terms of lower cost per liter and high marginal revenue therefore, fans with showers would be a quite useful technique for progressive farmers and also necessary from animal’s health point of view. Also, small scale farmers may use fans to avoid drastic decrease in milk production and take maximum achievable measures according to their affordability during mid and later summer.
Availability: Items available for loan: UVAS Library [Call number: 2490-T] (1).
42.
Impact Of Digestible Lysine, Methionine + Cysteine Ratios On Growth Performance, Ileal Digestibility Of Protein And Gut Health In Broilers Fed Different Protein Sources
by Ch. Ahsan Akram (2009-VA-397) | Prof. Dr. Talat Naseer Pasha | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry production is a vibrant segment of agriculture in Pakistan. Genetic selection and nutritional improvements resulted in accelerated growth rate and better FCR in modern broilers. This improved broiler performance requires greater amount of digestible nutrients. Since feeding cost accounts about 70% of total production cost and protein is the most expensive component of poultry diets. Future poultry diets, therefore, will be formulated by using cheaper and poorly digestible protein sources such as CM and SFM due to price volatility of highly digestible but expensive sources like SBM. The undigested protein in hind gut is subjected to microbial fermentation, results in formation of nitrogenous products that are not only harmful to birds, but also excreted into litter bedding and generate ammonia, nitrous acid and nitrous oxide, the latter one is a powerful greenhouse gas with an effect about 300 times compared with carbon dioxide. The potential solution to this problem is supplementation of synthetic amino acids in low nutrient density (CP) diets formulated by using cheaper and poorly digestible protein sources. Therefor a study was designed with the objectives to determine the impact of dLys, M + C ratios on growth performance, apparent Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM). An experiment with 432 mixed sex (Ross 308) 1-d-old broilers was conducted to test the hypothesis that supplementation of Meth may improves growth performance, apparent Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM). The interaction effects of the protein source (CM vs. SFM), CP levels (high vs. low) and dM+C/d Lys (recommended vs. supplemented) were tested in a factorial arrangement of 8 (2 × 2 × 2) dietary treatments. The supplementation of Meth positively influenced FI (P = 0.042), BWG (P = 0.003) and FCR (P <0.001). The broilers fed Meth supplemented diets had, on an average, 10% greater apparent Ileal digestibility of protein, 34% longer villi, 24.5% shorter crypts and 60% greater VCR compared with those fed diets with recommended levels of Meth. The levels of CP, similarly, influenced (P <0.001) the FI, BWG and FCR. The broilers fed high CP diets had 9% improved apparent Ileal digestibility of protein compared with those fed low CP diets. In conclusion, supplementation of Meth improves the broiler growth performance, apparent Ileal digestibility of protein and gut health fed poorly digestible protein sources (CM and SFM). The negative effects of poorly digestible protein sources thus may be partially ameliorated by supplementation of Meth.
CONCLUSIONS
In conclusion, the negative effects of poorly digestible protein sources thus may be ameliorated by supplementation of Meth. Supplementation of Meth above the recommended levels improves the growth performance, Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM).
SUGGESTION AND RECOMMENDATION
Further research is needed to evaluate the different strategies to improve the digestibility of poorly digestible protein sources including guar meal. Threonine also has an important role in gut health so there is a need to study the effects of supplementation of threonine above the recommended levels in broilers fed poorly digestible protein sources. Feed form has pronounced effects on gut development, digestibility of nutrients and subsequent growth performance of birds. There is a dire need to evaluate the other cheaper protein sources, supplemented with synthetic amino acids and to find out a possible interaction between feed form and poorly digestible protein sources that may replace SBM in broilers.
Availability: Items available for loan: UVAS Library [Call number: 2567-T] (1).
43.
Effect Of Methionine And Mannan Oligosaccharides Supplementation On Growth Performance, Carcass Characteristics, Immunity And Selected Blood Metabolites In Broiler Chicken Under Conditions Of High Ambient Temperature And Humidity
by Asif Mahmood (2009-VA-379) | Dr. Muhammad Afzal Rashid | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry sector is one of the most organized and vibrant segment of agriculture sector in Pakistan and is claimed to be among the largest industries of Pakistan with over more than 200 billion rupees investment and offering employment directly or indirectly to 1.5 million people. Broilers exposed to high environmental temperature and humidity exhibit several physiological and performance related setbacks. Heat stress cause high mortality, poor FCR, reduced feed consumption, body weight gain, and visceral development. Heat stress has been linked to a decrease in intestinal immune activity and to impaired intestinal morphology following pathogenic bacteria invasion of the body through the intestinal epithelium. Nutritional manipulations with its low cost are preferred to alleviate the negative effects of heat stress on performance. Nutritional strategies such as the addition of prebiotics, some amino acids e.g. methionine are reported to be beneficial in alleviating heat stress. As a nutritional intervention, synthetic methionine added to the diet has been explored as method of ameliorating the effects of ROS associated with high environmental temperature. Keeping above in view, the present study has been planned to evaluate the effect of supplementing methionine and MOS on the growth performance, carcass characteristics and immune status against ND under field conditions. In this experiment a total of n=200 day old chicks were procured from a commercial hatchery and randomly assigned to dietary treatments with n=40 chicks per treatment. Each treatment was further divided into four replicates with n=10 birds/replicate. Dietary treatments were : 1) negative control (NC) without any supplementation, 2) positive control (PC) containing antibiotic, 3) MOS supplemented (MOS) containing 800 and 400 g/ton of MOS in the starter and grower, respectively, 4) High Methionine supplemented treatment (HM) containing Methionine 1% of the diet in both starter and grower feeds, 5) Methionine and MOS supplemented (HM+MOS) treatment containing high methionine
SUMMARY
42
(1% in starter and grower) along with 800 and 400 g/ton of MOS in the starter and grower, respectively. The temperature and humidity were recorded daily at 8:00, 14:00 and 18:00 h and means were calculated to determine the daily changes in temperature humidity index. Feed intake, body weight and FCR were recorded at the end of each week. At the end of experiment n=3 birds per replicate were selected, weighed and slaughtered. At the time of slaughtering blood samples were collected in evacuated tubes. Serum was harvested after centrifugation, dived into aliquots and stored at –20 oC until further analysis. Serum samples were used to determine antibody titer against ND. At the time of slaughtering organ weighed and jejunal sample were collected to determine the gut morphology. The data collected were analyzed using analysis of variance (ANOVA) technique in a Completely Randomized Design (CRD). Results were declared statistically significant at P < 0.05 and compared using Tukey, s multiple comparison test. The statistical analysis were carried out using SAS 9.1 (SAS Institute, 2006). Live BW was higher (P<0.05) in HM+MOS compared to NC and HM. Feed intake was higher (P<0.05) in HM+MOS and PC than those in NC. However, FCR was lowest in HM+MOS compared to NC and PC birds. Relative organ weights were similar (P>0.05) among treatments; however, bursa of fabricius was heavier in MOS compared to the NC and HM+MOS. Villus height and villus to crypt ratio was highest (P<0.05) in HM, than the NC treatment birds CONCLUSIONS In conclusion, supplementation of methionine at 1% of diet and MOS improved the weight gain, feed intake and feed conversion ratio in birds reared under conditions of high temperature and humidity. Gut morphology of the bird also improved by supplementation of Methionine and MOS in the broiler diet.
SUMMARY
43
SUGGESTION AND RECOMMENDATION
Further research is needed to re-determine the recommended levels of methionine in the diet when bird are reared under the conditions of high temperature and humidity. Availability: Items available for loan: UVAS Library [Call number: 2606-T] (1).
44.
Protease Supplementation Influences Growth Performance, Gut Health And Ileal Digestibility Of Protein In Broilers Fed Low Protein Diets
by Shahzad Ahmad (2008-VA-240) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Proteases are protein-digesting enzymes that are used to break down the proteins of various plant materials and proteinaceous anti-nutrients in plant protein feedstuffs. The use of exogenous proteases can help in the reduction of the protein contents of a given feed by improving the digestibility and availability of the protein thereby reducing the cost of feed contributed by the protein feedstuff. It will also help to reduce the nitrogen excretion by the poultry and will contribute to mitigate the climate change.
In the present study the protease from a bacillus lachniformis spp was supplemented to evaluate the effect of enzyme on protein digestibility, gut health and birds performance. For this three experimental diets with 21, 19 and 17 % CP (HP, MP and LP) with six treatments with and without exogenous protease were used in this trial to evaluate the effect of protease. Each treatment was replicated to 6 pens and 10 birds were allocated to each pen. Parameters studied regarding birds performance were feed intake, weight gain and FCR. All the growth performance parameters were recorded on weekly basis while the Ileal digestibility and gut health parameters were recorded at the 35th day of the trial. Data was analysed through completely randomized design (CRD) under factorial arrangement using ANOVA technique (Steel et al. 1997). Means were separated through Duncan’s Multiple Range test (Duncon’s, 1955) with the help of SAS 9.1.
The results of feed intake, weight gain and feed intake: gain on weekly basis showed inconsistent results, however the overall performance of the birds fed low protein diets supplemented with protease showed significant results. The gut health parameters showed the significant increase in villus height. The apparent Ileal digestibility of protein was significantly increased in the MP supplemented with protease enzyme.
36
CONCLUSION
Based upon the findings of this study it can be concluded that;
Supplementation of protease improves growth performance of the broilers however the results on weekly basis were inconsistent but overall growth performance data shows significant results.
Protease supplementation enhances the CP digestibility in 19 % diets compared to 21 % and 17 % CP diets.
The supplementation of protease enzyme improves the villus height and crypt depth: villus height in broilers.
SUGGESTIONS AND RECOMMENDATIONS
Future researchers can further evaluate the subsequent effects of the protease on growth performance and immune response in broilers.
In future the research can be conducted to determine the effect of protease on energy digestibility and immune response in broilers. Availability: Items available for loan: UVAS Library [Call number: 2601-T] (1).
45.
Efficacy Of Different Microbial Phytases On Growth Performance Carcass Characteristics And Mineral Retention In Broilers
by Muhammad Faisal Rafique (2014-VA-918) | Dr. Talat Naseer Pasha | Dr. Saima | Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Cd Crupt Availability: Items available for loan: UVAS Library [Call number: 2762-T] (1).
46.
Effect Of Feed Form And Particle Size Of Corn On Growth Performance, Protein Digestibility And Gut Health Of Broiler
by Shahzada Muhammad Zeshan Haider (2014-VA-919) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The presented study was conducted at control house of Department of Poultry Production, C-block, UVAS Ravi Campus. In total 480, day-old Hubbard Classic broiler chicks were procured from local hatchery, weighed at 1st day and randomly distributed to eight treatment groups in such a way that there were six replicates in each group and each replicate had 10 chicks. Eight isocaloric and isonitrogenous experimental diets were formulated. Half birds were offered crumble form of feed in whole life while half birds were offered crumble form in starter phase and pellet form in finisher phase. Four levels (<1, 1-1.5, 1.5-2 and 2-2.5mm) of corn particle size were used in each form of feed.Body weight and feed consumptionwere recorded weekly in order to calculate feed conversion ratio.Data collected were analyzed by ANOVA using factorial arrangement under CRD. Experimental means were compared using DMR test.
Birds fed diet having higher particle size (2-2.5mm) of corn had lower feed consumption, greater body weight gain and improved FCR compared to those fed smaller particle size during starter, finisher and overall phase. Further, birds fed pelleted feed had lower feed consumption, greater weight gain and improved FCR than those fed crumble form of feed.Further, different particle size of corn resulted in non-significant effect on villus height and crypt depth. Feed form also had non-significant influence on villus height and crypt depth of duodenum.Birds fed diet having higher particle size (2-2.5mm) of corn had higher apparent ileal digestibilityof protein than those fed smaller particle size. Feed form had non-significant effect on apparent ileal digestibilityof protein.
It can be concluded from the results that birds fed pelleted diet containing coarse particle size had improved growth performance and protein digestibility without adverse effect on gut health of broiler.
Availability: Items available for loan: UVAS Library [Call number: 2879-T] (1).
47.
Studies On Growth Performance And Total Tract Protein Digestibility In As Influenced By Different Dietary Inclusion Levels Of White, Par Boiled And Brown Rice Broken In Broilers
by Omar Javed (2007-VA-154) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima.
Material type: Publisher: 2017Dissertation note: Poultry sector is playing substantial role in agriculture sector of Pakistan. In the present era extensive research in poultry sector has brought vital changes which are accelerating growth and lowering feed conversion ratio. Nutritional improvements in the poultry dietsare improving the growth performance and nutrient digestibility. Poultry diets are majorly formulated cost effective for the sustainability of poultry industry and its consumers. For this reason, the main focus is on using nutritionally rich and locally available raw feed ingredients in broiler diets. Cereal grain are main energy sources used in broiler diets and among cereal grains maize is being used as a major energy source in broiler diets, but its availability, price fluctuations, incidence of mycotoxins are the major concerns and hence, a suitable alternate energy source is required. Rice broken may serve as the substitute, but, a little data is available about the efficacy of different types of rice broken at different dietary inclusion levels regarding growth performance and total tract protein digestibility studies. Therefore a study was designed with the objectives to determine the impact of different types of rice broken (white, parboiled and brown) at different dietary inclusion levels (10%, 30% and 50%) in replacement with maize on broiler growth performance and total tract digestibility of protein. An experiment with 1-d-old 600 Cobb-500 broilers was conducted to test the hypothesis that rice broken from different sources may successfully substitute maize in broiler diets. The effects of the energy sources (white broken rice, parboiled broken rice and brown broken rice) and dietary inclusion levels(10%, 30% and 50%) were tested by using one way ANOVA under Completely Randomized Design and red maize as a control diet was used. The broilers fed different energy sources had significant variation in body weight gain and feed conversion ratio. Better body weight gain and feed conversion ratio was observed in control group followed by white broken rice and lower body weight gain and highest feed conversion ratio was observed in birds fed brown rice broken in diets, similarly total tract digestibility of protein was observed higher in birds fed 50 % maize (control group) and birds fed white rice in diets up to 30% inclusion level and lower total tract digestibility of protein was observed in birds fed brown rice broken in diets. The results of the study showed that red maize is the most beneficial energy source used in broiler diets and white rice broken could be used as good alternative energy source as compared to other types of rice broken i.e. parboiled and brown.
CONCLUSIONS
In conclusion, white rice broken can easily substitute maize up to 30% dietary inclusion level in broiler but other types of rice broken i.e. parboiled and brown give comparatively good results in terms of growth performance and total tract protein digestibility up to 10% dietary inclusion level in broilers.
Availability: Items available for loan: UVAS Library [Call number: 2878-T] (1).
48.
Studies On Growth Performance And Total Tract Protein Digestibility In As Influenced By Different Dietary Inclusion Levels Of White, Par Boiled And Brown Rice Broken In Broilers
by Omar Javed (2007-VA-154) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima | Jibran Hussain.
Material type: Publisher: 2017Dissertation note: Poultry sector is playing substantial role in agriculture sector of Pakistan. In the present era extensive research in poultry sector has brought vital changes which are accelerating growth and lowering feed conversion ratio. Nutritional improvements in the poultry dietsare improving the growth performance and nutrient digestibility. Poultry diets are majorly formulated cost effective for the sustainability of poultry industry and its consumers. For this reason, the main focus is on using nutritionally rich and locally available raw feed ingredients in broiler diets. Cereal grain are main energy sources used in broiler diets and among cereal grains maize is being used as a major energy source in broiler diets, but its availability, price fluctuations, incidence of mycotoxins are the major concerns and hence, a suitable alternate energy source is required. Rice broken may serve as the substitute, but, a little data is available about the efficacy of different types of rice broken at different dietary inclusion levels regarding growth performance and total tract protein digestibility studies. Therefore a study was designed with the objectives to determine the impact of different types of rice broken (white, parboiled and brown) at different dietary inclusion levels (10%, 30% and 50%) in replacement with maize on broiler growth performance and total tract digestibility of protein. An experiment with 1-d-old 600 Cobb-500 broilers was conducted to test the hypothesis that rice broken from different sources may successfully substitute maize in broiler diets. The effects of the energy sources (white broken rice, parboiled broken rice and brown broken rice) and dietary inclusion levels(10%, 30% and 50%) were tested by using one way ANOVA under Completely Randomized Design and red maize as a control diet was used. The broilers fed different energy sources had significant variation in body weight gain and feed conversion ratio. Better body weight gain and feed conversion ratio was observed in control group followed by white broken rice and lower body weight gain and highest feed conversion ratio was observed in birds fed brown rice broken in diets, similarly total tract digestibility of protein was observed higher in birds fed 50 % maize (control group) and birds fed white rice in diets up to 30% inclusion level and lower total tract digestibility of protein was observed in birds fed brown rice broken in diets. The results of the study showed that red maize is the most beneficial energy source used in broiler diets and white rice broken could be used as good alternative energy source as compared to other types of rice broken i.e. parboiled and brown.
CONCLUSIONS
In conclusion, white rice broken can easily substitute maize up to 30% dietary inclusion level in broiler but other types of rice broken i.e. parboiled and brown give comparatively good results in terms of growth performance and total tract protein digestibility up to 10% dietary inclusion level in broilers.
Availability: No items available
49.
Evaluation Of Different Strategies To Improve The Dietary Nitrogen Efficiency In Lactating Dairy Cows In Pakistan
by Muhammad Imran (2005-VA-09) | Prof. Dr. Talat Naseer Pasha | Dr. Muhammad Naveed ul Haque | Dr. Muhammad Qamer Shahid.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The objectives of this study were to optimize the protein supplies and replacement of SBM with locally available ingredients in the rations of high producing Holstein Friesian cows at mid lactation. On the basis of these objectives, three experiments were conducted. Multiparous cows in mid-lactation received three treatments in a 3×3 Latin square design with a period length of 20 d. Number of animals used were nine in 1st and 3rd experiments and 12 in 2nd experiment (Table 6.1). The trials were conducted at a corporate dairy farm. When we compare the initial and final values of milk yield, milk fat and milk protein contents, there is not a big difference of our diets with that currently being practiced in Pakistan (Table 6.1). This also reveals that the experimental milk production was close to pre-experimental milk production indicating that a successful dietary transition was achieved. Table 6.1: Demonstration of parameters before and during the experiments Exp.
Cows
No.
Initial Parameters During Experiment Parameters
DIM Milk
yield (kg)
Milk
fat (%)
Milk
protein (%)
Milk
yield (kg)
Milk fat
(%)
Milk
protein (%)
1 9 113±25 32±4.1 3.65 3.25 29.7±3.1 3.70 3.27
2 12 153±44 23.3±2.1 3.99 3.34 24.7±1.8 3.98 3.31
3 9 109±19 34±3.7 3.71 3.19 30.7±2.5 3.64 3.21
Exp., experiment; DIM, days in milk In the 1st experiment, three dietary treatments were designed to provide similar energy and increasing supply of MP (g/d)—2371 (low), 2561 (medium), and 2711 (high). Increasing the MP supplies did not modify DMI; however, it increased milk protein, fat, and lactose yield linearly. Similarly, FCM increased (9.3%) linearly due to an increase in both milk yield (5.2%) and milk fat content (7.8%). Milk nitrogen efficiency decreased from 0.26 to 0.20, whereas, the
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102
metabolic efficiency of MP decreased from 0.70 to 0.60 at low to high MP supplies and it average value across the treatments was 0.64 (Table 6.2). In conclusion, increasing the MP supplies resulted in increased milk protein yield; however, a higher BUN and low MNE indicated an efficient utilization of dietary protein in low MP supplies. Milk nitrogen efficiency ranges from 20 to 30% in dairy cows at mid stage of lactation. Milk nitrogen efficiency increases slightly but linearly with the increase of dietary protein up to a certain level of supply of protein. At high protein levels of dietary protein MNE is low and vice versa. In the 2nd experiment, the response of balancing metabolizable Lys to Met ratio (3:1) in low protein diets was investigated. Three experimental diets; 1) LP−: low protein diet (13.6% CP) with imbalanced Lys to Met ratio (3.33), 2) LP+: low protein diet (13.5% CP) with balanced Lys to Met ratio (2.94) through HMBi; and 3) HP−: high protein diet (14.7% CP) without balancing Lys to Met ratio (3.39) in a 3×3 Latin square design were designed. Milk yield of LP- was 0.85 kg/d less as compared with the average milk yield of LP+ and HP-. Dry matter intake decreased by 0.7 kg/d in LP+ compared to HP- treatment whereas milk yield tended to be higher by 0.7 kg/d and protein yield by 23 g/d. Balancing the Lys to Met ratio by supplementing HMBi through feed increased feed, N, and MP conversion efficiencies to milk by 4.4, 1.6, and 13.1% respectively compared to the HP- diet. Similarly, 4% FCM was increased by 4.4% in LP+ diet as compared to HP- diet. Moreover, plasma urea concentration was numerically less in LP+ compared to LP- and HP- treatments whereas no effect was observed on plasma glucose and TG concentrations. In the 3rd experiment, three diets 1) Control: with low protein with SMB as a protein source, 2) SBMD: high protein diet with SBM as a major protein source and 3) CGMD: high
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protein diet with CGM as a major protein source. Increasing the protein supplies did not affect DMI, milk fat yield, and milk fat and lactose contents in SBMD and CGMD diets compared to the control diet. Similarly, MP balance and MP/NEL increased by 31.5 and 9.1%, respectively. Increasing the protein supplies tended to increase milk yield. Similarly, milk protein and lactose yield increased by 3.5 and 3.3%, respectively. Milk protein contents tended to increase by 1.5% in SBMD and CGMD treatments compared to the control. Increasing the dietary protein supplies increased FE in SBMD and CGMD treatments compared to control, whereas, MNE decreased by 10.9%. No effect was observed on DM, N and NEL intakes when SBM was partially replaced with CGM. Consequently, milk yield, milk components’ yield, milk composition and feed efficiency remained unaffected. Contrary to this, MNE decreased by 5% in CGM treatment compared to SBM. There were no dietary treatment effects on blood metabolites including BUN, glucose and TG concentrations, which means neither replacement of SBM nor concentration of protein in the diet affected the blood metabolites profile. There was no change in lactation performance of cows by the partial replacement of SBM with CGM. Therefore, SMB could be partially replaced with CGM with urea without affecting animal performance, and saving the feed cost. Table 6.2: Effects of experimental diets on different parameters Exp.
MP
efficiencies
Δ MP
efficiencies (%)
Δ MY
(kg)
Δ DMI
(kg)
Δ milk
fat (%)
Δ milk
protein (%)
1 0.64 14.3 5.20 0.10 7.80 5.30
2 0.65 11.6 1.20 0.70 3.93 1.50
3 0.68 9.85 1.10 0.20 2.18 1.10
Exp., experiment; MP, metabolizable protein; MY, milk yield; DMI, dry matter intake
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In conclusion, balancing Lys to Met ratio at low protein diets and partial replacement of SMB with CGM is a mean to improve the MNE and reduce feed costs. 6.1 Conclusion and Recommendations Diets with low MP supply result in high MNE and better utilization with low levels of BUN. Although there was less milk yield in low protein diets but utilizing efficiency was high. Low protein corn-soy-based diets supplemented with rumen protected Met (HMBi) result in increased utilization of protein and low levels of BUN. Partial replacement of SBM with CGM plus urea showed no change in DMI, milk yield. Milk nitrogen efficiency was slightly decreased in CGM diet as compared to SBM diet. Feed cost could be saved by replacing 35% SBM with CGM provided that RDP is balanced by using NPN sources. Diets should be given with possible lowest protein levels having balanced AA particularly Lys and Met, which should be 3:1. High levels of protein could result into increased emission of gases to the environment. Soybean meal replacement with CGM along with some NPN source results in similar outcomes. First strategy is the best out of three currently tested and it can save money. 6.2 Future Perspectives Studies must be conducted to investigate the effects of further lowering the dietary protein levels without affecting milk production in Holstein cows. It will help to improve the dietary N utilization for milk synthesis. The above-mentioned strategies can also be tried simultaneously for improved protein/N utilization in dairy cows. Lysine can also be tried along with Met to balance the low protein corn-soy-based diets. On the basis of RDP and RUP values, other ingredients can also be tried to partially replace SMB. Availability: Items available for loan: UVAS Library [Call number: 2920-T] (1).
50.
Moringa Oleifera As Feed Additive In Poultry: Influence On Production Efficiency, Meat And Egg Profile
by Shakeel Ahmad (2011-VA-542) | Prof. Dr. Anjum Khalique | Prof. Dr. Talat Naseer Pasha | Dr. Shahid Mehmood.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Phytogenic feed additives gained considerable interest in the strategic replacement of antibiotic growth promoters (AGP) after European regulations for animal feed production which imposed a complete ban on antibiotics in animal feed used as growth promoters. Natural phytogenic growth promoters modify animal metabolism and gut microbiota population and positively affect the production and growth performance. Research in the animal feed production has established the basis for better feed efficiency, desired carcass traits and manipulation of bioactive compounds in the poultry eggs and meat, which resulted in the idea of functional foods and phytogenic feeds additives. In response to the above challenges a lot of research on aromatic plants, herbs, and spices was conducted in comparison with conventional antibiotic growth promoters. The data were recorded and analysed regarding gut morphology, gut ecology, feed digestibility, nutrients availability and meat and eggs bioactive compounds index of meat and eggs. Moringa oleifera might be used as phytogenic feed additives on the basis of diverse volatile compounds present in its leaves, pods and roots. Moringa belongs to family Moringaceae and is commercially grown in many tropics and subtropics countries globally. Moringa oleifera leaves and pods are very nutritious and retain their nutrients even when dried and converted to leaf powder. They can be utilized in both fresh as well as dried forms. Moringa leaves contain bioactive compounds like β-carotene, Quercetin and selenium in addition to basic nutrients (crude protein, metabolizable energy, ether extract, and ash). Moringa leaf meal contains 29.7, 22.5, 14.7, 4.3, 2.7, 0.26, 10.6% and 7.86Mj/Kg of CP, CF, Ash, EE, Ca, P, NFE and ME respectively. On the basis of above nutritional and bioactive compounds Moringa oleifera leaves meet the needs of a growth promoter and phytogenic feed additive as lot of studies on antimicrobial, anti-coccidial, AGP replacer and feed ingredient attributes have been conducted. Even then, very limited data was available regarding its phytogenic feed additive attributes. On the basis of above addressed issues the below experiments were designed. For this purpose following experiments were planned and executed. In the first experiment, authors took 200 day old broiler chicks and assigned to four dietary treatments in a completely randomized design replicated five times having ten birds each. Four iso-caloric and iso-nitrogenous diets were formulated for both the starter as well as finisher phases of production and four levels (0, 0.5, 1.0 and 1.5%) of Moringa leaf meal (MLM) were used over and above. Data regarding growth performance were collected and subjected to Analysis of Variance (ANOVA) techniques under Completely Randomized Design and means were compared using Duncan’s Multiple Range (DMR) test. Growth was monitored by feed intake, body weight, total gain, FCR and liveability. Feed intake was linearly decreased whereas quadratic response was observed in FCR (P≤0.05). Body weight, total gain and liveability remained same among the treatment groups. Dressing percentage showed quadratic response and highest values was observed in the group offered 0.5% of supplementation, whereas all other parameters including live weight, carcass weight, digestive organs (liver, gizzard) and heart size showed no effect. Bioactive compounds β-carotene, Quercetin and selenium in breast meat samples were lineally increased resulting in higher DPPH values (P≤0.05). Cholesterol, SGPT, creatinine and glucose levels in serum and breast meat samples were linearly decreased resulting in improved animal as well as consumer health. Moisture level of breast meat samples was linearly decreased whereas all other nutrients including Crude protein (CP), Ash, Ether extract (EE) and all minerals were linearly increased with the supplementation level (P≤0.05). Newcastle dis□ease titers remained unchanged while linear increase in IBD titers was observed. In second trial Moringa oleifera pods meal was added in the diet (starter and finisher) with same levels 0, 0.5, 1.0 and 1.5%. Two hundred broiler birds were purchased from commercial market and assigned to four treatments with five replicates and ten birds per replicate. Feed conversion ratio and feed intake was decreased (P≤0.05) with the increase in the supplementation level, whereas total gain was improved. Dressing percentage was negatively affected (P≤0.05), whereas organs weights (liver, hear & gizzard) were improved with the supplementation levels. β-carotene and Quercetin content of broiler breast meat were respectively 0.08μg/100g and 8μg/100g breast meat samples. Cholesterol level was decreased whereas selenium content was significantly increased (P≤0.05) in the treatment groups. Highest DPPH radicals scavenging activity was recorded in the treatment groups. When proximate analysis of meat was done, the moisture, ether extract, crude protein and ash contents were decreased (P≤0.05). Moreover biochemical indicators SGPT, Creatinine, Glucose and cholesterol were recorded significantly lowest (P≤0.05) in the treatment groups. In the third experiment Moringa oleifera leaf meal was used as feed additive in layers diet to its impact on performance, bioactive compounds and nutrient profile of eggs. For this purpose two hundred, fifty weeks (50) old, HyLine W36 layers were purchased from the commercial market and assigned to four treatments in a completely randomized design with five replicates and ten birds per replicate. Four iso-caloric and iso-nitrogenous diets were formulated and Moringa oleifera leaf meal (MLM) was supplemented at 0, 0.5, 1.0 and 1.5% over and above of basal diet. Results of this experiment significantly affected the production. Egg mass, production percentage and FCR were significantly affected whereas total feed intake and egg weight remained unchanged (P≤0.05). Egg shape and yolk index showed a quadratic response, whereas Haugh units and shell thickness was linearly decreased (P≤0.05). Bioactive compounds like β-carotene, Quercetin and Selenium content were enriched up to the levels 4906 and 241 and 56.82 μg/100g of egg yolk respectively (P≤0.05). Linear decrease in the serum biochemical compounds SGPT, Glucose, Creatinine and cholesterol
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143
levels (serum and eggs) at 4th and 6th week of supplementation was recorded (P≤0.05). Antibody titers against Newcastle disease were also significantly improved
(P≤0.05). Nutrients and minerals profile of egg yolk was also significantly changed (P≤0.05). In experiment No 4, effect of Moringa oleifera pods meal was investigated on 200 HyLine W36 layer birds of 50 weeks age. Diets were formulated having same caloric and protein levels and Moringa oleifera pods meal was added at top with the dose levels of 0, 0.5, 1.0 and 1.5% of basal diet. The results of this study showed quadratic response on egg mass and FCR per dozen eggs whereas feed intake, egg weight and production percentage remained same. Haugh unit showed a quadratic response whereas shell thickness was decreased linearly in the treatment groups (P≤0.05). Antioxidants like, β-carotene, Quercetin and Selenium showed same trend as in the previous experiment. Serum biochemical profile (SGPT, Glucose, Creatinine and cholesterol) were improved along with lower cholesterol content in egg yolk (P≤0.05). Proximate analysis of egg yolk showed that moisture and ether extract was decreased whereas CP, Ash and minerals (Na, K, Ca, Mg, P) profile was improved with the supplementation (P≤0.05). Results of all these four experiments support our hypothesis that Moringa oleifera can be a good phytogenic functional feed additive and can even perform at these lower supplementation levels. Moreover it can also be stated that eggs and meat produced from Moringa oleifera leaf and pods supplemented birds may serve as a functional food.
Conclusion
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On the basis of above experiments, it can be concluded that Moringa oleifera leaf and pods meal could be used to enhance the growth and production performance of broilers and layer bird along with bioactive compounds, like antioxidants, minerals and vitamins index of meat and eggs which could meet the advance concept of phytogenic feed additives.
Suggestions and recommendations
Outcomes of the present study including positive impact on growth performance, carcass traits, serum biochemical profile, immunity in broilers, and production percentage, egg geometry, and egg quality in layers in addition to enrichment of β-carotene, Quercetin and selenium in meat eggs suggest that Moringa oleifera can be considered as phytogenic feed additive.
Recommendations for the farmers
Keeping in view the above study following recommendation are given for the poultry producers
1. Moringa oleifera on the basis of its availability and nutrient profile can be a good phytogenic feed additive but there are multiple limitations most important are anti-nutritional compounds like saponins, phytosterols and high fibre and ash content in the leaves and pods meal inclusion in the feed.
2. Moringa pods are available once in a year and its availability is scarce so proper storage should be monitored.
3. Depending on the soil composition and climatic condition and varieties the nutrient especially ash content and bioactive compounds differ in its concentration so proper validation of nutrients is required before supplementation.
4. Supplementation is dependent on the nutrient profile so proper protocol and care must be considered while its use.
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5. In present study it is suggested that Moringa oleifera could be an option to be used as phytogenic feed additives to meet the condition including ban on AGP, better growth, production performance and safe and healthy meat and eggs.
Suggestions for the researchers
1. In the present study four levels of supplementation were used but future researchers can explore different levels in their studies.
2. Comparative studies with synthetic antioxidants, carotenoids and organic selenium can be designed.
3. Effect on shelf life can be studied in the animal products can be investigated in future studies.
Subsequent effect on acceptability of consumers regarding effect on the health including cholesterol levels, hypertension and cancer diseases can be studied. Availability: Items available for loan: UVAS Library [Call number: 2940-T] (1).
