1.
Geographical Distribution Of Dengue Fever Infected Patients And Its Related Risk Factors In And Aroun Lahore.
by Muhammad Usman Zaheer | Dr. Muhammad Hassan Mushtaq | Prof. Dr. Mansur-ud-Din Ahmad.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Dengue fever is the most common mosquito-borne viral infection of humans worldwide and is a major public health concern, especially in tropical and subtropical regions (Mackenzie et al., 2004). This life threatening disease is caused by a specific virus (ARBO-Flavivirus) transmitted by the bite of a female mosquito (Aedes aegyptii and Aedes albopictus) which is identified by the white bands and scale patterns on its legs and thorax. Trans-ovarial transmission of dengue virus has also been reported in different studies. The mosquito may bite at any time but the probability of bites increase at dusk and dawn (WHO 2009).
Dengue fever has emerged as a significant public health problem in Pakistan. In Pakistan, the first epidemic of dengue fever was reported in 1994 in Karachi (Anaari et al., 2001). Several years later, in 2006 another epidemic was reported in the southern part of Pakistan, predominantly caused by DENV-2 that led to 58 deaths (khan et al., 2008). In 2007, 22 people died of disease and in 2008 only five people were reported to die from it. Between October 2006 and January 2008, there were 3,242 laboratory confirmed cases of dengue fever in Pakistan. Since January 2009, 224 suspected cases were reported in Sindh province, of which 135 were the confirmed cases. During the epidemic of dengue fever in 2010 more than seven thousand cases were reported in Pakistan, of which 5 thousand cases were from Punjab and resulted in 63 deaths.
The mosquito-borne viral hemorrhagic diseases are prevalent throughout the world. Dengue hemorrhagic fever (DHF) is one of the most important diseases widely distributed in the tropics of South East Asia. It is an infectious malady of human beings characterized by fever and non-specific constitutional symptoms. The virus is transmissible to human beings by the bite of female Aedes aegypti and Aedes albopictus. The disease is spreading like a geometric rise of cases in a wide range of geographical regions.
The geographical factors and locality of patients may be a contributing factor to the outbreak of Dengue Fever. Climate change is likely to increase the area of land with a climate suitable for dengue fever transmission, and that if no other contributing factors were to change, a large proportion of the human population would then be put at risk.
The data on address of patients were collected from all the patients admitted in tertiary care hospital from August 01, 2011 to November 30, 2011. The geographical risk factors studied in this project were presence or absence of parks, trees, ponds, swimming pool, service station, tyre shop, source of stagnant water, pond and municipality garbage tank in vicinity of house of the cases and controls. For the study of risk factors we made 2 groups i.e. cases and controls; each comprising of 300 subjects and then data on presence or absence of various hypothesized risk factors in the vicinity of house (400 meters) and area (800 meters) were collected through a well structured and pre-tested questionnaire (Harrington et al., 2005; Honório et al., 2003; Schatzmayr 2000; Muir and Kay 1998; Reiter et al., 1995; McDonald 1977; Hausemann 1971 and Bond et al., 1970).
The statistical analysis of data was done by using SPSS 16.0. Qualitative data were presented in form of tables, bar charts, column and pie- charts. Chi-square test was applied to see the association between dengue fever and various hypothesized risk factors. P-value<0.05 was considered as significant. Odds ratio was calculated at 95% level of confidence (CI) for various hypothesized risk factors of dengue fever.
The results of present study revealed that the season of dengue fever in Pakistan is from August to November and the period prevalence of dengue fever to be 0.11%. Males (59%) were mainly the victim of dengue fever. The main affected age groups were 16-30 year (37.33%) and 31-45 years (29.67%). As far as the geographical distribution of dengue fever infected patients is concerned, it was concluded that dengue fever cases were reported from all the ten towns of Lahore metropolitan, however, most of the cases were reported from Ravi town followed by Data Ganj Baksh town and Cantt.
The results regarding various hypothesized risk factors are as follows: parks/trees/plants in vicinity of house OR=8.81, p-value <0.05; swimming pool in vicinity of house OR=5.O3, p-value <0.05; source of stagnant water in vicinity of house OR=8.25, p-value <0.05; fogging in your area OR=0.50, p-value <0.05; service station in the area OR=5.98, p-value <0.05; tyre shop in the area OR=6.11, p-value <0.05; municipality garbage tank in the area OR=5.88, p-value <0.05; any pond in the area OR=5.69, p-value <0.05.
From the results of this project we concluded that dengue fever is most prevalent from August to November and it is a disease of mainly male gender and mostly affects people of age group 16-45 years. Besides this, dengue fever is almost equally distributed in all the towns of Lahore metropolitan. Also we concluded that presence or absence of parks, trees, plants, swimming pool, stagnant water, pond, service station, tyre shop and municipality garbage tanks in vicinity of house are the risk factors of dengue fever. Fogging proved to be a deterrent to dengue fever.
On the basis of results of this project there is need to implement a control program in which vector control and minimizing the exposure of people to risk factors should be the key points that need to be addressed.
Availability: Items available for loan: UVAS Library [Call number: 1444,T] (1).
2.
Effect Of (Estradiol Benzoate) On Estrus Response And Fertility In Cidr Treated Crossbred Heifers.
by Muhammad Usman Mehmood | Dr. Mian Abdul Sattar | Dr. Muhammad | Prof. Dr. Nasim Ahmad.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Commercial dairy farming in Pakistan is rapidly growing. Crossbred dairy animals hold promise because of two reasons, first that they are significantly high producer than buffalo, Sahiwal, Cholistani and Desi cows and secondly that they are much more adaptable as compared to exotic (Friesian or Jerseys) cows. Generally, replacement heifers remain ignored by the owners in term of feeding and early achievement of puberty as they are not producing. Estradiol promotes tighter synchrony and ovulation and provides opportunity for fixed time A.I as reported earlier in Holstein dairy cows. Use of CIDR has been reported to be effective for synchronization in variety of dairy animals including buffaloes, dairy and beef cows. However no such report has been published on crossbred heifers. The objectives of the present study were to investigate the effect of estradiol benzoate (EB) on estrus intensity, response and fertility in CIDR treated cross bred heifers.Crossbred heifers with average body condition of 3 ± 0.5, located at four farms were assigned throughout one year to receive one of the two treatments. Crossbred heifers with EB (n = 50) or without EB (n = 50). All the heifers were treated with Controlled Internal Drug Releasing Device (CIDR; 1.38 mg Progesterone; Pfizer, USA) on day 0 and were administered injection of PGF2?, (0.150 mg; Dalmazine®, Fatro, Italy; 2 ml; i.m.) on day 6 followed by removal of CIDR on day 7. Estradiol benzoate (400µg/4ml, i.m. Sigma Aldrich Germany) was administered 24 h after the CIDR removal. Estrus detection was carried out by visual observation; twice daily for at least 30 min, beginning after CIDR removal till insemination. All heifers were fixed time inseminated with frozen-thawed semen after 48 and 60 h after CIDR removal, respectively. Pregnancy status was determined by ultrasonography (HS 1500V; Honda; Japan, with 7.5 MHz probe) between days 30 and 40 post AI. Estrus response and pregnancy rate were analyzed using Chi-square test. Pregnancy rate was determined by number pregnant/number treated x 100. The estrus response was 100% in both the groups. The estrus intensity was higher 2.9 ± 0.1in the EB heifers compared to the controls 2.0 ± 0.7. The results showed that pregnancy rate was 54% (27/50) in EB and 36% (18/50) in without EB heifers. The overall pregnancy rate was 45% (45/100). It is concluded that estradiol benzoate in CIDR protocol intensified the signs of estrus without affecting the response, with higher fertility in crossbred heifers.
Availability: Items available for loan: UVAS Library [Call number: 1502,T] (1).
3.
Identification Of Snp Markers For Egg Productionand Egg Quality Traits In Aseel And Naked Neck Chickens
by Muhammad usman | Dr..Atia Basheer | Prof. Dr. Muhammad Akram.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1962,T] (1).
4.
Pathological Studies On Contagious Edthyma In Naturally Infected Small Ruminants
by Muhammad Usman ghani | Dr. Mati ur rehman khan | Dr. Muhammad | Prof. Dr. Asim aslam.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2070,T] (1).
5.
Enhancing Fertility Through Induction Of Ovulation In Mares
by Fayzan sultan awan | Dr. Muhammad Usman Mehmood | Prof. Dr. Aneela | Prof. Dr. Nasim Ahmad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2161,T] (1).
6.
Molecular Epidemiological Analysis Of Pbi-F2 Protein Of Influenza A Virus Reported From Pakistan
by Muhammad Usman Basharat | Dr. Muhammad Hassan Mushtaq | Dr. Aqeel | Dr. Mamoona Chaudhry.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2179,T] (1).
7.
Effect Of Lactic Acid Hot Water And Tap Water Spraying On Microbiological Load And Shelf Life Of Beef Carcasses
by Muhammad Usman Badar | Dr. Muhammad Nasir | Dr. Zubair Farooq | Dr.Nasir Ahmed.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2184,T] (1).
8.
Comparison of The Effect of Ovsynch and G6G Synchronization Protocols on Ovulation and Pregnancy Rate in Nili-Ravi Buffaloes
by Muhammad Waqas (2008-VA-289) | Dr. Muhammad Usman Mehmood | Dr. Qaiser Shahzad | Prof. Dr. Mian Abul Sattar | Dr. Muhammad Afzal Rashid.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Thesis submitted with blank CD Availability: Items available for loan: UVAS Library [Call number: 2267-T] (1).
9.
Comparative Efficacy Of Anthelmintics Against Gastrointestinal Nematodes In African Lion (Panthera Leo)
by Muhammad Usman Munir (2008-VA-141) | Dr. Aneela Zameer Durrani | Dr. Muhammad ijaz.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: This study was conducted to compare the efficacy of Zental ( Albandazole) and Vermox (Mebandazole) against gastrointestinal nematodes like toxocara canis, toxocara mystax and toxascaris leonina in captive african lion (Panthera leo) kept in Lahore zoo, Lahor zoo safari, Bahawalpur zoo and Zain wildlife breeding farm Lahore because captive African lions are prone to different parasitic infestations.
A survey was carried out to estimate the prevalence of gastrointestinal nematodes in captive African lion and it was found that toxocara canis, toxocara mystax and toxascara leonina are the most commonly prevailing gastrointestinal nematodes in captive African lions in above mentioned places. The overall incidence rate of toxocara canis was 82.5 %, of toxocara mystax was 60% and of toxocara leonina was 50% respectively.
The Anthelmintics trials were conducted on 14 animals which were divided into 2 groups A and B each having 7 animals.
Group A was treated with Zental (Albandazole), group B was treated with Vormox (mebendazole), The percentage efficacy of each drug was determined on the basis of reduction on number of eggs per gram of discharged in faeces at day 7, 10, 13, 16 and 22 after deworming.
In group A, decrease in EPG of toxocara canis in group A was 21.1% at day 7, 37.2% at day 10, 54.7% at day 13, 74.6% at day 16 and 88.8% at day 22. Decrease in EPG of toxocara mystax was 15.9% at day 7, 38.1% at day 10, 56.6% at day 13, 70.6% at day 16 and 82.7% at day 22.similarly decrease in EPG on toxocaris leonina was 15.6% at day 7, 29.2% at day 10, 45.6% at day 13, 61.1% at day 16 and 83.1% at day 22 respectively
In group B, decrease in EPG of toxocara canis in group A was 7.24% at day 7, 14.57% at day 10, 23.26% at day 13, 31.27% at day 16 and 38.08% at day 22. Decrease in
Summary
25
EPG of toxocara mystax was 16.38% at day 7, 22.4% at day 10, 29.6% at day 13, 34.3% at day 16 and 41.2% at day 22.similarly decrease in EPG on toxocaris leonina was 13.6% at day 7, 21.29% at day 10, 30.8% at day 13, 39.1% at day 16 and 47% at day 22 respectively.
Results showed that efficacy of Zental (Albendazole) against gastrointestinal nematodes in African lion was higher as compared to that of Vermox (Mebendazole). Also it was more effective than Vermox (mebendazole) against different parasitic stages of nematodes as evident from EPG count at various days after medication. Availability: Items available for loan: UVAS Library [Call number: 2270-T] (1).
10.
Evaluation Of Antiviral Activity And Embryonic Toxicity Of Momordica Charantia Against Newcastle Disease Virus
by Muhammad Usman Ahmed (2013-VA-565) | Dr. Muhammad Adil Rasheed | Dr. Aqeel Javeed | Dr. Imran Altaf.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Plant products play a vital role in the management of various ailments due to their therapeutic activity. A wide range of active phytochemicals peptides have been found to have therapeutic uses against various functionally and genetically diverse viruses. Newcastle disease virus causes respiratory diseases in humans, birds and other mammals, representing one of the foremost threats to public health.
In this study, the antiviral activity of Momordica charantia L. and Ribavirin against Newcastle disease virus was evaluated in-ovo. For each extract of the plant M. charantia and ribavirin 40 embryonated eggs were assigned to 8 groups containing 5 eggs in each group (six groups for antiviral, six groups for embryonic toxicity, and two groups were kept positive and negative control respectively) and marked them with lead pencil. The aqueous and ethanolic extracts of Momordica charantia L. was prepared by using soxhlet extraction technique. From the extract, six different dilutions i.e. 160mg/ml, 80mg/ml, 40mg/ml, 20mg/ml, 10mg/ml and 5mg/ml of the aqueous and ethanolic extracts were prepared in normal saline whereas; six different dilutions i.e. 15μg/ml, 20μg/ml, 25μg/ml, 30μg/ml, 35μg/ml and 40μg/ml of ribavirin were made in normal saline.
With ND virus the different concentrations of the extracts of plant were mixed and 0.2 ml of this suspension was injected to 9th to 10th day embryonated eggs along with positive and negative controls having only virus and normal saline correspondingly. Ribavirin, standard drug, was inoculated by following the mentioned manner. These inoculated embryonated chicken eggs were incubated at 370C and were checked after 12 – 72 hours. After 72 hours of post inoculation, all the eggs were chilled at 40C in fridge for overnight stretch of time and the allantoic fluid was collected.
Summary
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The embryo survival percentage, positive or negative spot haemagglutination activity and determination of virus titre by haemagglutination test confirmed the antiviral activity. The embryonic toxicity effects of Momordica charnatia aqueous and ethanolic extracts and ribavirin was assessed by merely inoculating the extracts of respective concentrations as used for antiviral activity in embryonated chicken eggs and incubating for 72 hours. The outcomes were analyzed by ANOVA by means of SPSS. Availability: Items available for loan: UVAS Library [Call number: 2384-T] (1).
11.
Molecular Characterization of Pakistani Common Leopard
by Muhammad Usman Ijaz (2012-VA-908) | Dr. Sehrish Firyal | Dr. Ali Raza Awan | Dr. Muhammad Tayyab.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: CD not available. Availability: Items available for loan: UVAS Library [Call number: 2379-T] (1).
12.
Effect Of Alpha Lipoic Acid On Post Thaw Quality Of Jack Semen
by Muhammad Umair (2009-VA-49) | Dr. Mushtaq Ahmad | Dr. Muhammad Usman Mehmood | Dr. Hammad Bin Rashid.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Improvement of post-thaw quality of Donkey (Equus asinus) semen is essential to augment the in-vivo and in-vitro fertilization rate and to be used for mule production. By the help of cryopreservation sperm cells can be stored for the long time but it causes lethal sub-lethal damage to the sperm. In most species including Donkey and horses sperm cryosurvival rates are not optimal because of its plasma membrane composition. One of the major cryopreservation damage is produced by Reactive oxygen species (ROS) generating oxidative stress caused by ROS are important for normal sperm function but in normal concentration. When they are produced in more quantity they cause damage to Acrosome, DNA and plasma membrane. . Alpha lipoic acid is a non-vitamin coenzyme that helps in significant metabolic and antioxidant functions in the body. Alpha lipoic acid has been reported to have extra functions by which they are able to synthesize vitamin C from its reduced form in the presence of glutathione. It is matchless among biological antioxidants, because it is equally lipid and water soluble. This allows it to nullify free radicals almost everywhere in the body, inside as well as outside the cells. Therefore, the objective of present study was to determine the effect alpha lipoic acid on post thaw quality of jack semen. Alpha lipoic acid scavenge on reactive oxygen species formed in semen during the process of cryopreservation, so it maintained good semen quality during post thaw. Two adult donkeys (Equus asinus) (4-6 years old) kept at animal shed Ravi campus pattoki were used in the study. All the animals were managed under optimal condition of feeding and management. Donkeys were offered green fodder with ad libitum supply of water. Semen collection was done twice a week (one ejaculate/collection) using an equine artificial vagina having temperature of 45-50 ºC. Five collection from each donkey were done (n=10). Ejaculates were filtered with muslin cloth to remove gel. Semen volume was measured by collecting semen in a graduated collection tube after
Summary
28
filtration and the sperm concentration was measured by using a phase contrast microscope (40 x, Nikon) and was scored with a coverslip and then immediately was kept in water bath having 37 ºC temperature after collection until evaluation and processing. Semen quality parameters like volume, concentration and motility were recorded. After initial evaluation, semen samples were extended with centrifugation extender in 1:1 and seminal plasma was removed after centrifugation. Supernatant was removed so that seminal plasma up to 20% will remain with sperm pellet and was maintained at 37 ºC temperature in water bath and was extended with extender having different concentrations of Alpha lipoic acid (0mM, 0.5mM, 1mM, 1.5mM, and 2mM) and cooled for 2 hours and then equilibrated for 2 hrs at 4oC. Then, French semen straws of 0.5ml capacity were filled with semen (100x106/straw). All semen straws were arranged on a rack and then placed at 4cm height above liquid nitrogen vapors for 10 minutes. Finally semen straws was put in liquid nitrogen, Total five replicates per donkey were performed. Now post thaw quality was checked in which various tests were performed, like %age motility, Acridine orange assay for DNA integrity, HOST for plasma membrane integrity, FITC-PNA/PI for viability and acrosomal integrity. It was expected that Alpha lipoic acid shown positive effect on post thaw quality of donkey semen, in the meaning of increased percentage motility, Less DNA damage during cryopreservation and incubation, Increased acrosomal and plasma membrane integrity. So alpha lipoic acid shown positive effect by counter acting on ROS during cryopreservation by reducing melondialdehyde production as indicated by MDA test carried out in this study. Results acquired from this study shown that an increase in sperm motility, plasma membrane integrity, DNA integrity, Acrosomal integrity, viability and survival was caused by ALA competences in energy production and anti-oxidant properties, when used at the concentration of 1.5mM. In summary, based on the results
Summary
29
of our study, it can be concluded that an optimal concentration (1.5mM) of ALA improved PMI, sperm motility and viability, minimize DNA damage and improved sperm survival. Availability: Items available for loan: UVAS Library [Call number: 2494-T] (1).
13.
Effect Of Age On Lipid Peroxidation Of Fresh And Frozen-Thawed Semen Of Nili-Ravi Buffalo Bulls
by Sohail Ahmed (2014-VA-917) | Dr. Muhammad Irfan-ur-Rehman Khan | Dr. Sajid Iqbal | Dr. Muhammad Usman Mehmood | Dr. Muhammad Ijaz.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Buffalo spermatozoa are rich in polyunsaturated fatty acids and prone to lipid peroxidation. Malondialdehyde (MDA) is a byproduct of lipid peroxidation and causes irreversible damage to sperm structure and function. In buffalo, blood plasma MDA level increases with age. Therefore, we hypothesized that MDA level in buffalo bull semen will increase with age and will affect the semen quality. The objective of the study was to compare MDA level and quality of fresh and frozen-thawed semen in aged vs. young Nili-Ravi buffalo bulls. Single ejaculate was collected on weekly basis for four weeks from aged (13.6±1.0 years; n=3) and young (3.4±0.3 years; n=3) Nili-Ravi buffalo bulls. MDA level was estimated through thiobarbituric acid assay (TBA) in fresh and frozen-thawed semen. The quality of fresh and frozen-thawed semen was estimated through sperm motility, viability, DNA and acrosome integrity. MDA level (nmol/ml) did not differ (P>0.05) between aged vs. young bulls in fresh (2.3±0.2 vs. 2.9±0.7) and frozen-thawed (53.1±2.8 vs. 48.4±2.6) semen, respectively. In fresh semen, sperm motility and concentration did not differ (P>0.05) in aged vs. young bulls; however, the volume of fresh semen increased (P<0.05), while sperm viability and DNA integrity decreased (P<0.05) in aged vs. young bulls. In frozen-thawed semen, sperm motility, viability, and DNA integrity decreased (P<0.05) in aged vs. young bulls. In frozen-thawed vs. fresh semen, MDA level (nmol/ml) increased within young (48.4±2.6 vs. 2.3±0.2) and aged bulls (53.1±2.8 vs. 2.9±0.7), while motility and viability decreased (P<0.05) within the age groups. In conclusion, 1) lipid peroxidation (MDA) does not increase due to age in buffalo bull semen, and 2) freezing causes increase in lipid peroxidation irrespective of age and deteriorates semen quality of Nili-Ravi bulls.
Availability: Items available for loan: UVAS Library [Call number: 2707-T] (1).
14.
Effect Of Insemination Timing Following 5 Vs. 7 Day Cidr + Co-Synch In Nili Ravi Buffalo Heifers
by Sajjad haider(2014-va-767) | Dr. Muhammad usman mehmood | Dr.Muhammad usman mehmood | Dr.Muhammad zahid tahir | Dr.Muhammad awais.
Material type: Publisher: 2017Dissertation note: Pakistan’s economy is agricultural based in which livestock has a major contribution. Livestock contributes 11.8% to total national GDP. Nili Ravi is established breed of Pakistan. Nili Ravi has high lactation yields (1800-2500 liters with a 6.5% fat) and their males are more suited to ploughing and drafting on dry plane land. Increased calving interval and estrus detection are the biggest bottleneck in achieving the goal “a calf per 13 months” and high conception rate in dairy animals ultimately this leads to in economic losses. To overcome the problem of poor breeding in buffalo various exogenous hormonal intervention have been made to control estrous cycle of buffalo. Several ovulation synchronization protocols offer to bypass the estrus detection and artificially inseminate the animal at pre-established time. Co-synch is modified form of ovulation synchronization. Incorporation of CIDR in Co-synch improves the efficacy of protocol. It is easier in management and cost effective.
Present study was designed to compare the efficacy of 5 or 7 day CIDR + Co-synch in Nili Ravi buffalo and find appropriate time of A.I in Nili Ravi using 5 or 7 day CIDR + Co-synch protocol. Study was conducted on Military Dairy Farm Okara. Selected animals (N = 80) were divided into two treatments. Treatment 1) 7 day CIDR + Co-synch (n =40): The animals received an intravaginal CIDR insert containing 1.38 g of progesterone (P4) for 7 days. On the day of CIDR removal, 150 µg of PGF2α was injected intramuscularly (IM); Half of these animals (n=20) received 100 µg of GnRH IM and TAI after 72 hrs of CIDR removal/ PGF2α administered, the remaining half (n=20) received GnRH and TAI after 84 hrs of CIDR removal/ PGF2α administration. Treatment 2) 5 day CIDR + Co-synch (n=40): The animals received an intravaginal CIDR insert containing 1.38 g of P4 for 5 days. The animals were administered 150 µg of PGF2α IM at the time of CIDR removal. Half of these animals (n=20) received 100 µg of GnRH IM and TAI after 72 hrs of CIDR removal/ PGF2α administration, the other half (n=20) received 100 µg GnRH and FTAI after 84 hrs of CIDR removal/ PGF2α administration. Data is analyzed using IBM SPSS Statistics 20 software program. Follicular growth rate, pre-ovulatory follicular size is analyzed through independent t-test. Interval from CIDR removal to ovulation and interval from GnRH to ovulation is analyzed by Mann Whitney U test. Pregnancy rate is compared by binary linear regression. Ovulation rate and estrus response is analyzed through Chi square. A probability level of (P < 0.05) is considered as significant.
The results of this study showed the pregnancy rates were high (P < 0.05) in buffalo heifers of subgroups of both treatments inseminated after 84 hrs of CIDR removal (50% in 5 day CIDR + Co-synch and 65% in 7 day CIDR + Co-synch) than the subgroup inseminated after 72 hrs interval of CIDR removal (30% in 5 day CIDR + Co-synch and 25% in 7 day CIDR + Co-synch).
Follicular growth rate (mm/day) was tended to be high in 7 day CIDR treatment as compared to 5 day CIDR treatment (1.5±0.3 vs. 1.3±0.4, P=0.06). Pre-ovulatory follicle size in 7 day CIDR + Co-synch was significantly high in animals inseminated after 84 hrs as compared to subgroup inseminated after 72 hrs interval of CIDR removal (12.29mm vs. 10.74mm, P < 0.05). Similar trend was found in 5 day CIDR + Co-synch, the subgroup inseminated after 84 hrs of CIDR removal had larger pre-ovulatory follicular size than subgroup inseminated at 72 hrs interval (10.63mm vs. 11.73mm, P < 0.05). Interval from PG/CIDR removal to ovulation in 7 day CIDR treatment was 99 ± 0.9 hrs while in 5 day CIDR treatment it was 96 ± 1.6 hrs which did not differ significantly (P = 0.14). The interval from A.I/GnRH administration to ovulation in 7 day CIDR + Co-synch was significantly lower in animals inseminated after 84 hrs as compared to subgroup inseminated after 72 hrs interval of CIDR removal (15±1.2 vs. 27.±1.4, P < 0.05). Similar trend was found in 5 day CIDR + Co-synch, the subgroup inseminated after 84 hrs of CIDR removal/PG administration had lower A.I/GnRH administration to ovulation interval than subgroup inseminated at 72 hrs interval (16.2±1.3 vs. 21.6±1.6, P < 0.05). Ovulation rate was significantly high (P = 0.05) in 7 day CIDR treatment (95 %) than the 5 day CIDR treatment (80%). The estrus response in buffalo heifers was not significantly different between the treatments (90% in 7 day CIDR vs. 80% in 5 day CIDR treatments, P > 0.05).
In conclusion higher pregnancy rates were achieved in 7 day CIDR + Co-synch when animals were inseminated after 84 hrs interval of CIDR removal/PG administration. Acceptable pregnancy rates were resulted when timed AI was done after 84 hr of CIDR removal/PG administration using 5 day CIDR + Co-synch regimen. However further studies with larger sample size may be carried out to establish a strong statistical analysis between 5 and 7 day CIDR + Co-synch while inseminating animals after 84 hrs of CIDR removal/PG administration. Results of this study suggest that high pregnancy rate is primarily attributed to larger pre-ovulatory follicular size and appropriate time of AI in relation to time of ovulation. This study provides a way out to large dairy farmer to increase fertility with optimized management.
Availability: Items available for loan: UVAS Library [Call number: 2778-T] (1).
15.
Relationship Of Estradiol And Progesterone With Standing Estrus, Size Of Preovulatory Follicle And Interval To Ovulation In Beetal Goats
by Dr. Muhammad Irfan-ur-Rehman Khan | Dr. Muhammad Usman Mehmood | Dr. Muhammad Avais.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The objective of the current study was to determine follicular dynamics and plasma
concentrations of ovarian steroid in response to single PGF2α, given randomly during the luteal
phase in Beetal goat. A total of seven Beetal goats were given a single dose of PGF2α at the
unknown day of luteal phase upon confirmation of corpus luteum via ultrasonography during the
breeding season (November). Follicular dynamics were monitored using 7.5MHz transrectal
transducer at every 12 h following PGF2α (0 h) until ovulation. Plasma samples for estradiol 17β
and progesterone were collected at 0, 24, 36, 48, 60, 72, 84 and 96 h. An apronized buck was
used at every 6 h to detect standing estrus. Relative to PGF2α, all goats (n = 7) exhibited onset of
standing estrus at 50.6 ± 4.8 h and ovulation occurred at 82.3 ± 4.0h. However, the onset of
standing estrus and ovulation staggered (P < 0.05) among the goats. The onset of standing estrus
after PGF2α varied (P < 0.05) among early (n = 3), intermediate (n = 2) and late (n = 2)
responding goats i.e., 44 ± 2.0 vs. 51 ± 3.0 vs. 60 ± 0 h, respectively. The ovulation time among
early, intermediate and late responding goats was 72 vs. 84 vs. 96 h, respectively. The peak
plasma concentration of estradiol 17β was observed 12 h prior to ovulation in the goats. Mean
diameter of ovulatory follicle and duration of standing estrus were similar among the groups.
The corpus luteum regressed rapidly following PGF2α in early responding goats followed by
intermediate and late responding goats. Although plasma concentration of progesterone did not
differ (P = .065) among early, intermediate and late responding goats, but the change in
progesterone concentration over time differed (P < 0.05) among the groups. In conclusion, this
study indirectly shows that the onset of standing estrus and interval to ovulation following PGF2α
may vary in Beetal goats due to follicular and hormonal dynamics during the luteal phase. Availability: Items available for loan: UVAS Library [Call number: 2947-T] (1).
