Comparayive Efficacy Of Chemotherapeutic Trails Against Hypodermosis In Cattle In Rajanpur
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Publisher: 2011 Dissertation note: Study was carried out in district Rajanpur from February, 2011 to April, 2011 to find out percentage of infestation and to study chemotherapeutic trails against warble fly infestation in cattle in three tehsils namely Rojhan, Rajanpur and Jampur of district Rajanpur. In each tehsil 200 cattle were examined to record clinical signs of hypodermosis. For treatment of affected animals drug trail were done with Trichlorophon (Tagafon Star,Pakistan) and Ivermectin (Imec, SJG, Pakistan) 1% subcutaneously @ 0.2mg/kg body weight. Study included percentage of infestation in cattle in the households, veterinary hospitals and private farms. Average percentage of warbles in cattle was calculated and found 10 %, 17% and 16.5% in Rojhan, Rajanpur and Jampur respectively. It was found that percentage of infestation was 12% to 18% in hilly areas as compared to semi-hilly areas with 8% to16% and riverine areas 8% to 18% warble fly infestation in cattle. It was observed that warble fly infestation in cattle was absent in some plane areas in tehsil Jampur.
The comparison of different chemotherapeutic trails against Hypodermosis in cattle were observed and recorded. Three groups of cattle were made for chemotherapeutic trails against hypodermosis. Application of Tagafon in two different groups of cattle was carried out to evaluate their efficacy against Hypodemosis. In one group Trichlorophon (Tagafon Star,Pakistan) was applied with cotton gauzes by swabbing and spraying method with 5% solution of trichlorophon. In second group Trichlorophon (Tagafon Star, Pakistan) was given by drenching method with 2% solution at a dose rate of 0.2ml/kg body weight. In third group (Inj.Imec, SJG, Pakistan) Ivermectin 1% at dose rate of 0.2mg/kg body weight subcutaneous injection was carried out. Ivermectin1% was found to be 100% effective. Tagafon (Trichlorophon) was found 90% effective by swabbing and spraying method with 5% solution and Tagafon (Trichlorophon) by drenching method with 2% solution was found 85%effective against warble fly infestation in cattle in the study area. The data was analyzed statistically and found significant.
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Prevalence Of Dermatophytosis In Domestic Dogs And In-Vivo Evaluation Of Topical Anri-Fungal Agents
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Publisher: 2011 Dissertation note: The pet dog is a domesticated animal, belonging to the family Canidae and it comes within the order Carnivora. The given nomenclature is used for all varieties of pet dogs. Earlier, this pet animal was used for different purposes which include hunting, fighting and also different types of work had been taken from it. Dermatophytosis is the common skin infection in dogs especially in the young ones. A total of 300 samples were collected. The 36 samples were found positive and then divided into 3 groups. In group K, Ketoconazole was applied topically twice a week. Later on the efficacy of Ketoconazole was observed by collecting the samples on day 7, 14 and 21 after the commencement of treatment, which was 20%, 60% and 90% respectively. Similarly, Group L was treated with Lime sulphur and the efficacy of Limesulphur against M.canis infection, was found to be 60%, 80% and 100% on day 7, 14 and 21 respectively. Group M was treated with Miconazole. The efficacy of miconazole against M.canis was found to be 50%, 75% and 100% on day 7, 14 and 21 respectively. At the end, it was concluded that Lime sulphur was the best drug of choice among the three drugs used and the efficacy of ketoconazole was lesser than the others.
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Determinaton Of Multiple Drug Resistance Against Pasteurella - Multocida Isolates From Cattle And Buffalo In District Naseerabad, Balochistan
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Publisher: 2011 Dissertation note: Hemorrhagic Septicemia is one of the major problems from livestock point of view with high morbidity, mortality, loss of production and cost of treatment. The predisposing factors such as high temperature, high humidity and uncontrolled transportation of cattle and buffalo round the clock favor outbreak from time to time in the District Naseerabad, Balochistan. Forty samples were collected aseptically from Cattle and Buffalo and transported for further processing placing temperature 4 0C in to the Laboratory Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore.
Samples were cultured on blood agar for isolation and purification .These isolate were identified macroscopic, microscopic and biochemical profile characteristics test. The antibiotic sensitivity test of Ceftriaxone, Cephaloridine, Trimethoprim, Streptomycin, Cinoxacin, Colistin, Ciprofloxacin, Norfloxacin, Chloramphenical and Amoxycillin were determined for P. multocida isolates and also observed noticeable resistance against these antibiotics. The collected data was tabulated and analyzed statistically.
The aim and objective of present study was to determine multiple drug resistance against Pasteurella multocida so that proper antibiotic for the above said ailment with recommended dosage may be accomplished according to standard dosage.
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Chemotheraph Of Ancylostomiasis In Cats In Lahore.
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Publisher: 2012 Dissertation note: It has been a long time since the affection of cats is with human beings. Cats have recorded as most adoptable pet of the modern world. Cats can be found anywhere in the world. Many diseases of cats are zoonotically important and they may cause zoonotic problem in human and mainly these pathogen are involved in enteric problems. The relationship of feline with human does provide many benefits to them but also act as a cause of zoonotic diseases.
A total of 300 fecal samples were collected from cats and were examined in the Clinical Medicine Laboratory, University of Veterinary and Animal Sciences, Lahore. The positive samples for Ancylostoma were 65 out of 300, indicating prevalence of Ancylostomiasis was 21.66%. For the chemotherapeutical trails 18 positive cats and 6 healthy cats were divided into four groups viz. A, B, C and D with 6 animals in each group. The cats of group A were treated with Pyrantel Pamoate @ 20mg/kg orally. The cats of Group B were treated with the Albendazole @ 50mg/kg orally. The cats of Group C were kept as positive control while the cats of Group D were kept as negative control.
The fecal samples were collected on day 0 pre-treatment and then on day 7th, 14th and 21st of post-treatment. The efficacy of the drugs was calculated on the basis of reduction of the eggs count from the fecal samples on the respective days. The efficacy of Pyrantel Pamoate on these days was 43.47%, 67.39% and 94.20% respectively, against Ancylostoma. The efficacy of Albendazole on these days was 30.77%, 60.44% and 86.81% respectively, against Ancylostoma. Thus it was recorded
that Pyrantel Pamoate was more effective against Ancylostoma than Albendazole as it caused more reduction of the egg count than albendazole.
Hematological study showed that the values of hemoglobin, packed cell volume and total erythrocyte count were lower in groups A, B and C as compared to non-infected control group. The values of total leukocyte count were higher in group A, B and C as compared to non-infected group. As a result of treatment in group A and B, the values of hemoglobin, packed cell volume and total erythrocyte gradually increased while that of total leukocyte count gradually decrease on day 7th, 14th and 21st post-medication. It has been observed during the present study that Pyrantel Pamoate and Albendazole showed their positive effect on blood parameters as a result of Ancylostomiasis with more efficacy of Pyrantel pamoate as compared to that of Albendazole.
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Prevalence And Chemotherapy Of Gastro-Intestinal Paradites In Deer In Punjab, Pakistan.
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Publisher: 2012 Dissertation note: The parasitic load on deer affects them badly and eventually leads to death. In the present study a total of 100 feacal samples were collected from deer and were examined in the Clinical Medicine Laboratory, department of CMS, University of Veterinary and Animal Sciences, Lahore. Out of 100 samples thirty eight were positive and among these thirty eight, 12 deer were infected with cestodes, 6 with nematodes, 12 with trematodes, and 8 with the mixed infection of parasites. So, the prevalence of the cestodes, trematodes, nematode and mixed infection was 12%, 12%, 6% and 8% respectively. For the chemotherapeutic trails thirty positive animals and 10 healthy animals were divided into four groups A, B, C and D with 10 animals in each. The animals of group A was treated with albendazole @ 7.5mg/kg orally. Those of group B was treated with the ivermectin @ 1mg/ 50kg orally. The group C animals were kept as positive control and group D were kept as negative control.
The feacal samples were collected on the 0 day (pre-treatment), 7th, 14th and 21st day (post-treatment). The efficacy of the drugs were calculated on the basis of reduction of the eggs count from the feacal samples on the respective days. The efficacy of albendazole 42.59 %, 71.3% and 87.96% against cestodes, 33.33%, 60.3% and 82.88% against nematodes and 40.18%, 68.22% and 89.72% against trematodes at day 7, 14 and 21 respectively.. The efficacies of ivermectin were 42.59 %, 73.15% and 88.88% against cestodes, 51.78%, 76% and 91.07% against nematode while 42.85%, 64.28% and 85.71% against trematodes at day 7, 14 and 21 respectively.
It is thus concluded that the ivermectin is more effective drug than albendazole as it caused more reduction of the egg count than albendazole.
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Prevalence And Chemotherapy Of Theileriosis In Equines In And Around Lahore
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Publisher: 2012 Dissertation note: Theileriosis is an infectious malady of equidae characterized by intermittent fever, loss of appetite, anemia, loss of weight, dyspnea, pale mucous membranes, jaundice and recumbency. Considering the significance and utilization of equines in our country and the substantial losses rendered by theileriosis, the present study was designed to study prevalence and chemotherapy of theileriosis in equines in and around Lahore.
For this purpose 300 equines (Horses=100; mules=100; donkeys=100) were examined for theileria infection.The blood was collected by ear tip puncture and from Jugular venipuncture. Then thin blood smear slide was prepared and dried up in air and stained with Geimsa's staining method and examined under microscope. Theileriaequiwere identified by their morphological characteristics as described by Rashid et al. (2009) and Guimaraeset al. (1997).Over all prevalence of Theileriaequiin equines was estimated as 58%. There was also a mixed infection of Theileriaequi and Babesiacaballiin some cases. Individually there was 54%, 64% and 56% prevalence of Theileriaequi found in horses, mules and donkeys respectively.
The effect of theileriosis on various blood parameters (Hb, ESR, TEC, TLC, DLC and PCV) was determined in 30 equines (Horses= 10; mules= 10; donkeys= 10). The remarkable decrease in hemoglobin, TEC, PCV and lymphocytes was observed. The value of ESR increased while there were no remarkable changes in TLC, monocytes, basophils, neutrophils and eosinophil.
For chemotherapy, 36 equines (Horses=12; mules=12; donkeys=12) positive for theileriosiswere divided into three groups (A, B, and C). Each group was further subdivided into three subgroups comprising of 4 animals in each subgroup. The animals of group A (Horses=4; mules=4; donkeys=4) were treated with Imidocarbdipropionate (Imizol®, ICI, Pakistan) @ 2 mg/kg BW I/M, and the efficacy of drug was found 91.7% in equines against theileriosis.The group B (Horses=4; mules=4; donkeys=4) was treated with Buparvaquone (Butalex®, ICI, Pakistan) @ 2 mg/kg BW I/M alone and was observed 66.7% effective. While the group C (Horses=4; mules=4; donkeys=4) was treated with Auk(Calotropisprocera)@ 0.3mg/kg BW, 8 doses at alternate days (local treatment), which was found 58.3% effective. The efficacy of drugs was measured on the basis of disappearance of clinical signs and blood smear examination at day 2, 4, 6 and 10 of post-medication. The efficacy of each drug was measured through recovery rate of the animals.
Finally, the data on prevalence of theileriosis was estimated by Pearson's chi-square test for significance whereas data on hematology was analyzed by Student's T-test using statistical software package STATA 9.1 (College Station T×77845, USA), P < 0.05 was considered significant.
Considering the significance and utilization of equine in our country and the substantial losses rendered by theileriosis, the present project was designed to check prevalence and chemotherapy of theileriosis in equines in and around Lahore. The results of this study will help farmers and veterinary practitioners in field.
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Prevalence And Chemotherapy Of Trichuriasis (Whi Worm) In Dogs.
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Publisher: 2012 Dissertation note: In developing countries like Pakistan the trend of establishment of kennel clubs are increasing day by day. While the population of dogs increase the strong practice of vaccination and deworming increase as well. Parasitism is a major problem in our country. It causes great economic losses. The genus Trichiuris is one of the important nematode which affect the dogs. Therefore a study of genus Trichiuris was conducted in month of March to May, 2012. For this purpose 300 hundreds dogs of different breeds of dogs were selected for investigation of Trichiuris vulpis. Samples were collected and brought to the Clinical laboratory of Department of Clinical Medicine and Surgery in University of Veterinary and Animal Sciences Lahore. In laboratory positive samples for Trichiuris vulpis were recorded. The overall prevalence of Trichiuris vulpis was found to be 20% .The dogs positive for Trichiuris vulpis were divided into three groups A, B and C having 10 dogs in each group. Group A and B were treated with pyrantal pamoate and fenbendazole respectively. While the group C was positive control. The efficacy of both the drugs were measured by the decrease in egg per gram of faeces after medication. The egg counts were made at day zero, 7th and 14th.
The efficacy of pyrantal pamoat recorded on day 7th and 14th was 95.30 and 98.61% respectively while the efficacy of fenbendazole recorded on day 7th and 14th was 90.76 and 93.84% respectively. The obtained data was tabulated and analyzed by ANOVA.
Statistical analysis showed that there was significant (p<0.05) difference between the efficacy of payrental pamoate and fenbendazole after treatment of infected dogs at day 7th and 14th. This analysis showed that payrantal pamoate is significantly superior in its efficacy than fenbedazole.
The purpose of the study for prevalence and estimation of comparative efficacy of two drugs against Trichiuriasis in dogs was accomplished.
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Srudy Of Mycotoxicosis In Cattle And Buffalo In District Sheikhupura, Punjab.
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Publisher: 2013 Dissertation note: Abstract
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Incidence And Hematological Study Of Trichomoniasis In Domestic And Wild Pigeons In And Around Lahore
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Publisher: 2015 Dissertation note: Poultry industry is the most effective and economical source of animal protein. Because of increasing future demands, poultry industry is unable to narrow down the animal protein supply and demand gap. Poultry producers are looking forward for using the alternate source of chicken meat, which in the future will come from pigeon and quail meat. It will be very help full for increasing gross domestic production (GDP) through livestock sector (Basit et al. 2006).
Pigeons have been domesticated to live close with human beings. Pigeons originate from the rock dove in Europe, have been partially domesticated and carried to all parts of the world. Pigeons have been divided into three groups.
1) Poultry pigeons
2) Carrier and racing pigeons
3) Fancy and feral pigeon
Pigeons are easily bred to produce a variety of plumage or to provide squabs for the table (Basit et al. 2006).
It is very well known that internal parasites cause great loss to the host, by different ways. These parasites live at the expense of host depriving them from the nutrients essential for their growth. Moreover they cause mechanical harm by producing inflammation and tissue damaged. Protozoa inhabiting the digestive tract of birds are responsible for considerable economic losses. Heavy infestation of the parasites affect the health of birds with loss in the body weight, retarded growth, unthriftiness, damage to the gut epithelium, fertility disturbances, emaciation and death especially in young birds (Urquhart, 1996).
Common name of Trichomonas gallinae is canker, frounce and roup. Predilection site of this parasite is esophagus, crop and proventiculus. It belongs to the family Trichomondidae and class Zoomastigophorasida (Taylor et al. 2007).
Body of T. gallinae is elongated, ellipsoidal os pyriform. Its size is 5-19 × 2-9 μm. It has four anterior flagella that arise from blephroplast, having undulating membrane that does not reach the posterior end of body and free posterior flagellum is absent. Its axostyle is narrow and protrudes 2-8 μm from the body and its anterior portion is flattened into a spatulate captulum. Its parabasal body is hook shaped and parabasal filament is present (Taylor et al. 2007).
The host of T. gallinae is pigeon, turkey, chicken and raptors (hawks, falcons and eagle). As the method of reproduction is concerned it reproduced by longitudinal binary fission. There is no sexual stages and cyst are present in its life cycle. Lesion present in the turkey and chicken are most commonly in the area of crop, oesophagus, pharynx and no lesion are found in mouth (Taylor et al. 2007).
Size of trophozoites of T. gallinae is about 7-11 μm. Its shape is varied from oval to pyriform. It has four flagella and a fifth recurrent one, which did not become free at the posterior pole. Size of nucleus is about 2.5–3 μm, oval in shape and situated closely below the basal bodies of the flagella. Its axostyle consisted of a row of microtubules that is running from the region of the apical basal bodies to the posterior end of the cell (Mehlhorn et al. 2009).
Infected pigeons show wild signs of depression, lose weight, stand huddled with ruffled feathers and may fall over when forced to move. There is an accumulation of greenish fluid present in the mouth and crop containing large number of trichomonads in it. Yellow, necrotic lesions are present in the esophagus and crop (Taylor et al. 2007).
Trichomonas gallinae is a causative agent of trichomoniais in birds. It affects mostly to the young birds and causes death in them, especially in pigeons within 10 days. This Protozoa is present in the gastrointestinal tract of birds and causes greater financial losses. Clinical sign of diseased birds are dull, depress and having yellow color diarrhea. Morbidity rate of this disease is high in birds. If infected birds are not treated, it causes high mortality in diseased birds, due to this reason this problem become very important. Trichomonas gallinae presents in upper digestive system and respiratory system. So it affects both digestive system and respiratory system. It is mainly found in pigeons, but turkey, chicken, hawks, mourning doves, golden eagles, falcons and bustards may also be infested with this protozoa (Saleem et al. 2008).
Small, whitish to yellowish caseous nodules are found in the esophagus, pharynx and crop. Their size increased and may remain circumscribed and separate, or may become thick, caseous, necrotic mass present in lumen. The circumscribed disk shape lesion are known as yellow buttons. Size of nodule is 1 cm or more and found in liver, lungs and other organs (Taylor et al. 2007).
Infection spread to turkey and chicken by drinking contaminated water. The pigeon and other wild birds are also source of infection, which also use the water source. T. gallinae enters in the water through mouth and not from feces of the wild birds. Source of infection is direct contamination because this organism is very sensitive to drying and no cyst are found (Taylor et al. 2007).
There are more chances of trichomoniasis in young one than in adult pigeons. During feeding carrier pigeons transmit this disease to their young ones. The pathological lesions associated with trichomoniasis is inflammation, ulceration, and necrosis in nature. These lesion are more predominant in the oral cavity, esophagus, crop and proventiculus (Kennedy et al. 2001).
This disease is present worldwide. A clonal strain of previously described organism has been recently developed as the cause of widespread disease of birds in Europe and causes a grate economic losses (Ganas et al. 2014). In Britain, this infectious disease was first described in 2005. This disease causes significant mortality in birds which results in decreased population of green finches and passenines (Robinson et al. 2010).
T. gallinae affects upper digestive system of birds and results in pathological changes in structure of different parts of digestive system. It causes slight inflammation of mucosa to large caseous lesions of esophages. These lesion sometime block the lumen of esophagus. Due to this secondary infection of parasites, bacteria and virus takes place in diseased birds. Different strains of parasites move toward other organs such as liver, air sacs, lung, and brain. They causes necrosis of these organ, which leads to the death of birds (De Carli et al. 2002).
Prevalence of T. gallinae infection is different in different age of birds. Prevalence increased with the age of nestling (Krone et al. 2005). A higher prevalence of Trichomoniasis has been recorded in pigeons in Pakistan season wise prevalence has been recorded to be 43%, being non significantly higher in April (56%) than in March (30%). Trichomoniasis positive cases show a significant decrease in hemoglobin concentration, number of monocyte, packed cell volume, body weight than healthy birds (Saleem et al. 2008).
T.gallinae is a parasite of different species of birds ranging columbiformes, diurnal raptors and captive gallinaceous birds. It causes stomatitis, esophagitis and ingluvitis (Bunbury et al. 2007).
This disease is recently emerged in British passerines. This parasite has caused high mortality in finch and their population is decreased and this disease is also spread to continental Europe (Chi JF 2013).
The sequence of T.gallinarum is different from Tetratrichomonas gallinarum that is another trichomonad of birds, but it is genetically similar to Trichomonas Caninistomae that affect dog and cat and causes oral infection. Pigeons are prey of dog, so there is possibility that T. gallinae may have infected (carnivorous) mammals in the past (Gasper et al. 2007).
This disease causes large economic loss of avain livestock and also cause problems for wild species of birds. In UK T. gallinae has caused the death of greenfinches (Lawson et al.2006). Trichomoniasis was first reported in 2005 in Britain. It was discovered in finches. It caused large scale mortality in finches with population decline (Robinson et al. 2010; Lawson et al. 2011). In 2007 this disease is reported in finches in the Canadian Maritime provinces, southern Fennoscandia and northern Germany (Lawson et al. 2011). This disease caused high morbidity and mortality in finch population in Britian. It is estimated that about 1.5 million greenfinches which represent the 35% of national population have been died with this disease (Lawson et al. 2011).
In this disease multiple foci of caseous necrosis is seen in oral, esophagus and crop mucosae. There is heavy infiltration of inflammatory cells especially heterophill in these areas. There is multiple foci of necrotic inflammation is seen on liver. Due to excessive infiltration of heterophils in there is thickening of mucosa of easophagus. Necrotic material is also seen in mucosa and submucosa of easophagus. There is necrosis on the tip of villi of intestine and necrotic materials is seen in the mucosa of intestine (Al Sadi et al. 2011).
Trichomoniasis occurred more frequently in young than adult pigeons. High prevelance of trichomoniasis have seen in male than female pigeons (Al Sadi et al. 2011). Nestling birds are more susceptible to this disease than other birds. In Tucson, Arizon study was conduct and this study show that T. gallinae was present in oral cavity of 85% nestling coopers hawks compared to
only 1% of breeding age hawks. This disease is more prevalent in young pigeons. T. gallinae is sensitive to environmental pH. Trichomonas gallinae develop well when pH is between 6.5 and 7.5 (optimum 7.2), but cannot survive in more acidic pH. In fledgling and breeding Coopers Hawks pH of their oral cavity is acidic, so they are less susceptible to trichomoniasis and this is very important in differential prevelance among age group of birds (Urban et al. 2014).
Trichomonas gallinae changes the blood picture of infected birds. It causes decreased values of Hb, PCV and monocytes in infected pigeons than the healthy pigeons, while TLC, heterophils, lymphocytes and eosinophils are increased in disease pigeons than the healthy pigeons (Seddiek et al. 2014).
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Effects Of Aflatoxin M1 (Afm1) On Sheep And Efficacy Of Mycotoxin Binders
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Publisher: 2015 Dissertation note: Livestock production is an important part of national economy and it plays a significant role in providing the high quality food for human beings. Dairy industry of Pakistan contributes up to 46.8% in the agriculture and about 10.8% of the GDP in the form of milk, milk products, meat, hides, skin and bone meal (Ghaffar et al. 2007). Molds are fungi consisting of filaments that are present in the feed of animals. Molds can cause many infections in dairy cattle, especially during period of stress when their immune system is depressed. They cause a special disorder that is termed as Mycosis. Specific mycotoxins are produced by these fungi and when these animals eat such type of feed then they also engulf such dangerous mycotoxins. Mycotoxins are produced by a wide range of various molds and can be classified as secondary metabolites meaning that their function is not essential to the mold’s existence (Whitlow and Hagler 2005).
In the European countries, sheep milk is mainly produced in the Spain, and it is about 403000 tons per year. The main purpose of this milk is to produce cheese. Manchego cheese is considered as the best quality cheese in the world. This is the only technique by which we can produce the high quality of cheese (Rubio et al. 2009). These Aflatoxins are the fungal main dangerous constituents that can contaminate the feed of animals very easily. If AFB1 contaminated ration is consumed by animals then metabolism takes place in these toxins and excrete AFM1 in milk which further reduces milk production. More long-lasting aflatoxin poisoning produces very dissimilar signs that may not be clinically superficial and decreased rate of production in young animals. Severe aflatoxicosis causes hepatitis, hemorrhage, and death. Decreased growth rate is the most delicate clinical symbol of chronic aflatoxicosis, and it may be the only readily obvious abnormality. Natural toxins considerably show the greater danger to human and animal health. One large group of natural toxins that are universally
documented as poisons of food and feed are the mycotoxins. The toxins occur naturally in various animal feeds, including corn and cottonseed. To control AFM1 in milk, it is essential to decrease the feed contamination by AFB1 (Prandini et al. 2009).
The livestock is playing major role in enhancing agricultural productivity and it has also major contribution to decrease the rate of poverty in the rural areas of Pakistan. Most farmers have domesticated sheep in their home which are their major sources of income (Mahmood et al. 2009). Aflatoxins adulterated rations prompted important reduction in daily feed consumption and the means of body weight, body weight increment and feed transformation rates were radically affected during the exposure stage to aflatoxins. Furthermore, serum ingredients and ruminal measurements showed lessened liver function and digestive turbulences in sheep fed aflatoxin (Jouany and Diaz 2005)
Aflatoxins are included in that group of toxins which are considered as highly toxic toxins. Aspergillus parasiticus, Aspergillus flavus and Aspergillus nomius are those types of fungi which can produce very strong type of toxins. Aflatoxin B1 (AFB1) is considered as the most dangerous type of aflatoxin and it is very strong hepato-toxin (damaging the liver as a whole). It can also cause cancer, can produce teratogens, and may also cause mutation in the both animals and in human beings.
AFB1 being very toxic, can be activated by the special metabolism of liver and through cytochrome P450. The cancer causing ability of AFBI is ten times more than the AFM1. The conversion of AFB1 to AFM1 in the milk producing animals is in the range of half percent to about six percent. In milk producing animals the level of aflatoxin M1 is greatly altered by the contamination rate of aflatoxin B1 (Bognanno et al. 2006).
It is observed that the excretion pattern of aflatoxin M1 in the milk of sheep is lower than the excretion pattern of aflatoxin M1 in the milk of cows. Due to presence of aflatoxin B1 in the feed of animals and hence appearance of aflatoxin M1 in the milk of animals shows that
milk of such animals becomes more toxic and more un-hygienic. The young ones having milk as their main feed ingredient may have much more chances of getting various infections. (Var and Kabak 2009). To check the level of aflatoxin M1 in the milk of sheep, various experimental studies have been performed in the whole world (Battacone et al. 2005). In Pakistan, there are many favorable environmental conditions in which various types of fungi can easily prompted (Iqbal et al. 2011). The contamination level of aflatoxin M1 in the milk of dairy animals and in the dairy products has been found by (Hussain et al. 2008).
Aflatoxins can be produced from fungal metabolites. Aflatoxin M1 is produced as the complete metabolism of aflatoxin B1 has taken place. Aflatoxin B1, when converted into aflatoxin M1 is appeared in the milk of that animals and the same aflatoxin M1 can also be appeared in the dairy products which can contaminate the feed of human too. Transformation of aflatoxin B1 into the aflatoxin M1 is around one to two percent. This transformation is highly dependent upon the feed of animals, transformation from one affected animal to other healthy animal, transformation from the milking of affected animal to the milking of healthy animal and from one day to another day also. It is also noted that as the level of aflatoxin B1 in the feed of animal is decreased down then the level of M1 in the milk of animals is also dropped down significantly but it takes about 72 hours for this dropped down (Nilchian and Rahimi 2012).
AFM1 had a resistant to thermal inactivation used during food processing procedure such as pasteurization and autoclaving. Storage of various dairy products was not effective in the reduction of this toxin (Ozdemir 2007). Aflatoxin M1 level in the milk of animals can also be found in 12 to 24 hours after the ingestion of aflatoxin B1. After this, its level can be raised within the few days. The estimation of conversion rate of aflatoxin B1 in the contaminated feed of animals into aflatoxin M1 in the milk that animal is elaborated as 1 to 3 percent in a specific range (Ozdemir 2007).
Aflatoxin B 1 being very much dangerous mycotoxin, it is very much important to know about such methods by which the level of aflatoxin B1 in the feed of animals can be controlled. For this purpose we should first come to know the basic source of feed contamination of animals, and after this we should adapt such easy method to determine the level of aflatoxin B1 in the animal’s feed and such methods should be very cost effective. The best accepted and recognized method to determine aflatoxin M1 level in the milk of infected animals is HPLC high-performance liquid chromatography and TLC thin-layer chromatography (Thirumala-Devi et al. 2002). Aflatoxin M1 can be found in the milk of infected animals and can also be found in the products that are prepared from that contaminated milk. This contamination is the main problem arising now a days in the whole world (Fallah et al. 2009).
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Effect Of Different Treatment Trials On The Carrier Status Of Streptococcus Equi In Horses Recently Recovered From Strangles
Material type: Book ; Format:
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Publisher: 2015 Dissertation note: Strangles is characterized by upper respiratory tract infection, dysponea, anorexia, regional suppurative lymphadenitis causing high morbidity and low mortality in horses as well as in mules and it is an infectious problem of equine. Considering the significance and utilization of equines in our country and the substantial losses rendered by Strangles, the present project was designed to study epidemiology, diagnosis and chemotherapy of strangles in Lahore, Okara and Sargodha districts of the Punjab province in Pakistan. Streptococcus equi subspecies equi is highly prevalent in animals recently recovered from strangles and antibiotics along with immune stimulants and non steroidal anti-inflammatory drug became helpful in the elimination of carrier status of animals for S. equi. Samples were collected from animals recently recovered from Strangles. Samples were collected with the help of sterile cotton swabs dipped in normal saline from nasopharynx & oropharynx followed by culturing of samples on blood agar plates which were incubated anaerobically for a time period of 24-48 hours respectively and S. equi were isolated on the basis of colonies characteristics and growth pattern. Streptococcus equi was confirmed with the help of Gram staining and biochemical tests Catalase reaction, Methylene blue reduction test and Sugar fermentation test. In vitro antibiotic sensitivity test were also performed to select three antibiotics showing best efficacy against S. equi. Carrier animals were subjected to treatment with the help of antibiotic along with combination of immune stimulants and non steroidal anti-inflammatory drug. Carrier status was considered eliminated with disappearance of S. equi from nasopharynx. Blood samples were collected from carrier as well as from healthy animals to check out hematological parameters such as TLC (total leukocytes count), TEC (total erythrocytes count) and MHC (mean hemoglobin concentration) etc. Out of
hundred samples collected from Sargodha, Lahore and Okara districts of Punjab the prevalence of S. equi in horses and mules was 15%. Highest prevalence in equines was found at Remount Depot Mona, was recorded which is 18.3% followed by Okara 13.33%. No case was found to be positive for Strangles out of ten samples collected at UVAS, Lahore.
In-vitro antibiotic sensitivity test was performed on above isolates and it was found that ceftiofur Na was found to be most effective drug followed by norfloxacine and ampicillin. In vivo treatment trials showed that Group C animals treated with ceftiofur Na, norfloxacine, and ampicillin along with Vit E & Selenium supplemented by Phenyl butazone were found to be negative for post treatment carrier status. This study had aided in diagnosis as well as in treatment of strangles and was also provided us with the understanding of hematological parameters.
Data on prevalence of S. equi in carrier animals were analyzed by Chi square test.
While comparison of different treatments trials was done by Z test.
Hematological parameters were analyzed by mean ± SED using SPSS software 16.0.
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A Study On The Correlation Of Serum Electrolytes And Trace Elements Along With Associated Risk Factors In Diarrheic Foals
Material type: Book ; Literary form:
Publisher: 2015 Dissertation note: Foal diarrhea is one of the major threats to equine industry that causes high morbidity and mortality. Electrolytes (Na, K, Ca, Fe, Cu and Li etc) and water losses reduced dietary intake and probable translocation between intracellular and extra cellular compartments. Electrolytes are essential for controlling membrane potential, muscle contraction, nerve condition, and enzyme reactions, and they play a central role in the physiological processes. Serum electrolytes and trace elements deficits are the major causes of fluid and acid-base imbalance in diarrheic foals. Keeping in view the importance of foal diarrhea and its impact on the equine industry the current study was designed to determine serum concentration of electrolytes (Ca, K, Na) and trace elements (Li, Cu, Fe) along with the associated risk factors in diarrheic foals.
The present study was designed to examine diarrhea in foals at various equine stud farms, private and public veterinary hospitals. For this purpose a total of (n=100 foals) suffering from clinical diarrhea irrespective of the cause of diarrhea selected randomly after clinical examination and (n=10 foals) negative for diarrhea as control were included. Data of each diarrheic foal was collected separately in a data capture form. Blood and serum sample were collected for hematological and serum biochemical studies, respectively. Concentrations of serum electrolytes (Ca, Na, and K) were analyzed by Flame Photometry and trace elements (Cu, Li, and Fe) were estimated through Atomic Absorption spectrophotometer. PCV was determined by micro hematocrit method. Risk factors including species and age were analyzed for association.
Foals which were suffered from diarrhea showed increase in Pack cell volume. Serum concentration of Sodium, potassium, calcium, iron, copper and lithium were measured in diarrheic foals by atomic absorption and flame photometer method and were compared with healthy group of foals.Foals having diarrhea showed an acid baseimbalance as a result decrease in serum electrolytes and trace elements. The blood acid-base balance is precisely controlled because even a minor deviation from the normal range can severely affect many organs. The body uses different mechanisms to control the blood's acid-base balance.Sodium and potassium and calcium are important electrolytes imbalance in diarrhea. Potassium level was increased in foals having diarrhea. Calcium concentration in ppm significantly decreased from its normal value. In some diarrheic foals increased in level of calcium was also observed because these foals have blood in their feces that result in the calcium to come in blood as a clotting factor. Trace elements such as Cu, Fe and Li are needed in a very little concentration for many physiological functions. The serum iron was measured in foals having diarrhea.The iron concentration was less than normal value.The deficiency of iron lead to anemia. Clinical signs of anemia because of irondeficiency are rarely seen in foals. Therefore, mineral imbalances influence iron metabolism and may cause iron deficiency in foals. The serum copper concentration was significantly decreased in the diarrheic foals. Copper concentration is also related to the packed cell volume as in anemia copper deficiency is a significant feature. Copper is important element and its deficiency lead to decrease in serum concentration of iron. This decreased level might be because most of the circulating copper in plasma is attached to the serum glycoprotein, ceruloplasmin. Ceruloplasmin has ferroxidase activity and may be required to deliver iron into the circulation Therefore it was observed that low iron concentrations might have resulted from copper deficiency.The concentration of Lithium was also decreased in diarrheic foals when compared to healthy foals.
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Seroprevalence And Molecular Detection Of Brucellosis In Animals In Mirpur, Azad Kashmir Pakistan
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Publisher: 2015 Dissertation note: Brucellosis is declared as one of the most widespread zoonoses in the world by the world's reknowned organizations. It is defined as a contagious systemic bacterial disease primarily of ruminants. The disease is manifested by late term abortions, weak calves, still births, infertility and also associated placentitis, epididymitis and orchitis, with excretion of the organisms in uterine discharges and milk. With the intensification of the import of animals and the establishment of big farms in the last few years, the incidence of brucellosis increased sharply in many countries, both in man and animals.
In this study 360 serum samples were examined from four groups of animals in district Mirpur Azad Kashmir. Blood samples of 3ml from buffaloes, cattle, sheep and goat (n=30) each were taken from three sub-divisions of Mirpur separately. The serum samples were screened by RBPT which is a screening test for brucellosis, and it was observed that 8.6% animals were seropositive by RBPT. The serum samples of cattle were 17.8%, buffalo were 8.9%, goat were 2.2%, and sheep were 5.6% positive respectively.
The serum samples positive by RBPT and some randomly taken samples were further confirmed by the use of most specific and sensitive serological test known as i-ELISA. 6.87% animals were confirmed as seropositive by i-ELISA including cattle (17.5%), buffalo (10%), sheep (0%) and goat (0%).
All RBPT positive samples were further subjected to RT-PCR. Among these 31 samples 24 were positive for Brucella genus and only 7 samples were negative. Samples were further tested for confirmation of Brucella species. All 24 samples were having Brucella abortus.
The data were analyzed by using the Statistical package for the social sciences (SPSS) program version 22. Data were analyzed using Chi-square test and Z-test statistics.
Availability: Items available for loan: UVAS Library [ Call number: 2378-T] (1).
Experimental Treatment Of Bovine Brucellosis Using Phyto-Chemo-Immuno Theraputic Agents
Material type: Book ; Literary form:
Publisher: 2015 Dissertation note: Bovine brucellosis is very important zoonotic disease in respect of outbreak that resulted in reproductive loses through storm of abortions in herds and infertility in bulls. Currently, no phyto treatment for complete recovery from brucellosis is available.
Phyto Chemo-immune therapeutic agents are effective for recovery from bovine brucellosis (Hypothesis)
Group A was comprise five adult cows (n=5) positive for brucellosis. Animals in this group were given 3 shots of Oxytetracycline LA at 20mg/kg BW (IM) repeated every 48 hrs. Streptomycin @ 13mg/kg BW IM was given for six consecutive days. Furthermore, flunixin meglumine @ 2mg/kg BW IM for 3 days in combination with antibiotic. Selevit injection containing selenium and vitamin E was administered (IM) for consecutive five days. At next day all the animals in this group were vaccinated with a combined vaccine of RB51 and FMD (Oil based) at a dose of 3mL/animal through deep IM route. In group B, all the animals were given Saafi (Herbal Product) orally consecutively for six days. Furthermore, flunixin meglumin @ 2 mg/kg BW IM was administered for three days. All these treatments were given simultaneously. Then injection Selevit containing selenium and vitamin E were administered (IM) for consecutive five days. After treatment all animals were vaccinated with RB51 + FMD (oil based) 3mL/ animals deep intramuscularly. In group C, flunixin meglumin @ 2 mg/kg BW IM was administered for three days. After NSAID, Selevit injection containing vit. E and selenium was administered (IM) for five days regularly. After treatment, animals were vaccinated with RB51 + FMD (oil based) 3 mL/ animals (IM). In D group five negative adult animals were included in this group and vaccinated subcutaneously with RB51 and FMD (Oil based) at a dose of 3 mL/animal.
Data regarding this study was analyzed with ANOVA and completely randomized design was used to compare the mean between different groups using statistical package for social science (SPSS) version 20. P < 0.05 was considered significant.
The results showed OD values after 1st, 3rd and 6th month were significantly different in all groups. The mean values depicted that OD values was significantly higher (2.886±0.099) in vaccinated (positive) animal’s vs the other three groups. There was no significant difference in OD values of herbal treated, antibiotic treated and health control animals. The lowest OD values was found in healthy control animals, though significantly similar with herbal treated, antibiotic treated. The mean OD value at 3rd month after treatment is significantly higher (1.794±0.090) in herbal treated and vaccinated positive groups but the OD value of healthy group was lowest (1.794±0.090) after 3rd month of treatment. After 6th month of treatment the OD value of positive vaccinated group was found significantly highest (1.146±0.194). On the other hand lowest value (0.595±0.079) was found in healthy control group.
All the groups have found non-significant difference in all group. Regarding Real time PCR the value range from 0-40 Ct values. In the present study the Ct values of the control positive was found to be 13 which indicate control positive. The antibiotic treated group had a Ct value found in this range 35.61 to 38.13. The herbal treated group were all positive and the values were 31.34 to 37.42 Ct. The vaccinated immune booster group. The Ct values in the group was found to be 30.83 to 34.21. The birth weight and placenta dropping time was normal in antibiotic treated animals. There were no significant results found in all groups.
This study concluded that herbal regimen is effective in vitro against brucellosis but in vivo it is yet not to be evaluated. The antibiotics can be effective to treat the Brucellosis with this protocol. This was help to control bovine brucellosis, and extensive economic losses. Comparative efficacy of four commonly-immune therapeutic agents were help in choosing the most effective therapy/method for the recovery of bovine brucellosis.
Availability: Items available for loan: UVAS Library [ Call number: 2388-T] (1).