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Efficiency Of Nitrogen Utilization In Response To Level Of Crude Protein Supplies In Primiparous Lactating Nili-Ravi Buffaloes

By: Saba Anwar (2006-VA-68) | Dr. Muhammad Naveed ul Haque.
Contributor(s): Dr. Fayyaz Ahmed | Prof. Dr. Anjum Khalique | Dr. Muhammad Qamar Shahid.
Material type: materialTypeLabelBookPublisher: 2015Description: 35p.Subject(s): Department of Animal NutritionDDC classification: 2453-T Dissertation note: Background: In lactating buffaloes the protein requirements essentially depend on milk production & milk protein content together with the efficiency of conversion of dietary nitrogen to milk nitrogen. Lactating buffaloes utilize crude protein efficiently but still excrete some nitrogen (N) in feces. This inefficient N conversion results in increased feed cost and environmental pollution. There is need for the estimation of optimum level of protein required for lactating buffaloes for its maximum utilization and minimum wastage. Hypothesis: To check the efficiency of 3 different levels of crude protein this is better utilized for milk production of buffaloes. Methodology: The experiment was carried out at BRI, Pattoki. Six (6) Nili Ravi primiparous buffaloes in mid lactation were used for this experiment. Animals received 3 treatments in a 3×3 latin square design with a 21 days (d) period (total duration of experiment will be 63d). The treatments contain diets are 9% (CP9), 11% (CP11), and 13% (CP13) of crude protein content. Diet distributed as well as orts (refused) was noted on daily basis. Fodder samples were collected after every week in each period for evaluating the DM and for proximate analysis. Concentrate and wheat straw was analyzed once in each period. Milk yield of buffaloes were recorded daily and milk samples were collected for determining composition of milk. Body weights were taken on fortnightly basis. Blood samples were collected for analysis of different physiological metabolites. Statistical Analyses: Data was analyzed through General Linear Model by using PROC GLM command (SAS, 2004). Further polynomial linear and quadratic regression were used to determine the effects. Outcome: It was concluded from the study that high protein intake in the diet did not increased the DMI and milk parameters except fat contents. Level of BUN increased at high CP diet which depicts the inefficiency of nitrogen utilization by buffaloes. Buffaloes are less efficient in nitrogen utilization than cows. The low protein treatment appears sufficient to support milk production of 9 kg/d.
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Background:
In lactating buffaloes the protein requirements essentially depend on milk production & milk protein content together with the efficiency of conversion of dietary nitrogen to milk nitrogen. Lactating buffaloes utilize crude protein efficiently but still excrete some nitrogen (N) in feces. This inefficient N conversion results in increased feed cost and environmental pollution. There is need for the estimation of optimum level of protein required for lactating buffaloes for its maximum utilization and minimum wastage.
Hypothesis:
To check the efficiency of 3 different levels of crude protein this is better utilized for milk production of buffaloes.
Methodology:
The experiment was carried out at BRI, Pattoki. Six (6) Nili Ravi primiparous buffaloes in mid lactation were used for this experiment. Animals received 3 treatments in a 3×3 latin square design with a 21 days (d) period (total duration of experiment will be 63d). The treatments contain diets are 9% (CP9), 11% (CP11), and 13% (CP13) of crude protein content. Diet distributed as well as orts (refused) was noted on daily basis. Fodder samples were collected after every week in each period for evaluating the DM and for proximate analysis. Concentrate and wheat straw was analyzed once in each period. Milk yield of buffaloes were recorded daily and milk samples were collected for determining composition of milk. Body weights were taken on fortnightly basis. Blood samples were collected for analysis of different physiological metabolites.

Statistical Analyses:
Data was analyzed through General Linear Model by using PROC GLM command (SAS, 2004). Further polynomial linear and quadratic regression were used to determine the effects.
Outcome:
It was concluded from the study that high protein intake in the diet did not increased the DMI and milk parameters except fat contents. Level of BUN increased at high CP diet which depicts the inefficiency of nitrogen utilization by buffaloes. Buffaloes are less efficient in nitrogen utilization than cows. The low protein treatment appears sufficient to support milk production of 9 kg/d.

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