101.
Comparayive Efficacy Of Chemotherapeutic Trails Against Hypodermosis In Cattle In Rajanpur
by Muhammad Zahid | Dr. Aneela Zameer Durrani | Dr. Muhammad Hassan Saleem | Prof. Dr. Azhar.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Study was carried out in district Rajanpur from February, 2011 to April, 2011 to find out percentage of infestation and to study chemotherapeutic trails against warble fly infestation in cattle in three tehsils namely Rojhan, Rajanpur and Jampur of district Rajanpur. In each tehsil 200 cattle were examined to record clinical signs of hypodermosis. For treatment of affected animals drug trail were done with Trichlorophon (Tagafon Star,Pakistan) and Ivermectin (Imec, SJG, Pakistan) 1% subcutaneously @ 0.2mg/kg body weight. Study included percentage of infestation in cattle in the households, veterinary hospitals and private farms. Average percentage of warbles in cattle was calculated and found 10 %, 17% and 16.5% in Rojhan, Rajanpur and Jampur respectively. It was found that percentage of infestation was 12% to 18% in hilly areas as compared to semi-hilly areas with 8% to16% and riverine areas 8% to 18% warble fly infestation in cattle. It was observed that warble fly infestation in cattle was absent in some plane areas in tehsil Jampur.
The comparison of different chemotherapeutic trails against Hypodermosis in cattle were observed and recorded. Three groups of cattle were made for chemotherapeutic trails against hypodermosis. Application of Tagafon in two different groups of cattle was carried out to evaluate their efficacy against Hypodemosis. In one group Trichlorophon (Tagafon Star,Pakistan) was applied with cotton gauzes by swabbing and spraying method with 5% solution of trichlorophon. In second group Trichlorophon (Tagafon Star, Pakistan) was given by drenching method with 2% solution at a dose rate of 0.2ml/kg body weight. In third group (Inj.Imec, SJG, Pakistan) Ivermectin 1% at dose rate of 0.2mg/kg body weight subcutaneous injection was carried out. Ivermectin1% was found to be 100% effective. Tagafon (Trichlorophon) was found 90% effective by swabbing and spraying method with 5% solution and Tagafon (Trichlorophon) by drenching method with 2% solution was found 85%effective against warble fly infestation in cattle in the study area. The data was analyzed statistically and found significant.
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102.
Diagnosis And Treatment Of Strongylosis Using Neem Leaves And Ivermectin In Donkeys
by Muhammad waqas | Dr. Muhammad sarwar khan | Dr. aneela zameer durrani | Dr. Muhammad.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2011Dissertation note: Donkeys act as draught animals and are used for a variety of purposes. Donkeys are still the "beasts of burden". Helminthes parasites, particularly strongyle nematodes, are the common inhabitants of the gastro-intestinal tracts of equines. Strongylosis is one of the most important parasitic diseases of equines. Diarrhea, anorexia, weight loss and moderate anaemia are the clinical signs in infected animal resulting in heavy mortality. The present project was therefore designed to investigate the infection rate of nematodes in donkeys, to calculate the efficacy of Ivermectin and Neem against strongylosis and to study the effect of strongylosis on various blood parameters (DLC and Hb).
Three hundred donkeys (n=300) were examined coprologically for the presence of nematodes. 167 donkeys were found infected with various parasites with the overall infection rate being 55.66%. Out of 167, 85 donkeys were infected with strongylus, 29 with trichostrongylus, 19 with trichonema, 19 with gastrodiscuss and 15 with mixed infection. So the infection rate for strongylus, trichostrongylus, trichonema, gastrodiscuss and mixed infection was 28.33%, 9.66%, 6.33%, 6.33% and 5% respectively. For drug trails thirty donkeys naturally infected with strongylosis were selected. These animals were divided into three groups A, B and C, comprising of 10 animals each. Another group D comprising of 10 healthy animals was also made. Group A was treated with ivermectin at the dose rate of 0.2 mg/kg body weight (1 ml/50 kg b w) once. Group B was treated with neem dry leaves at the dose rate of 375 mg/kg body weight mixed with wheat bran for four days. Group C was kept as positive control (Infected-Untreated) and Group D was kept as negative control (Uninfected-Untreated).
The faecal samples were collected on day zero pre-medication and on day 7 and 14 post-medication. The efficacies of ivermectin and neem were calculated on the basis of reduction in number of eggs per gram (EPG) of the faeces. The efficacy of ivermectin was 73.21 and 96.42% on day 7 and 14 respectively. The efficacy of neem was noted to be 22.22 and 33.33% on day 7 and 14 post-medication respectively. It was concluded that ivermectin was most effective. The blood samples were also collected on day zero (pre-medication) and on day 7 and 14 (post-medication) and were examined for hematology. Groups A, B and C had low haemoglobin values. The values get improved in groups A and B after treatments but the value further decreased in group C because this group was kept as infected control. The Hb value remained normal in group D as the animals in this group were healthy. In group C there was significant increase in eosinophils.
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103.
The Efficacy Of Chemotherapeutic Agents Against Paramphistomum In Sheep
by Muhammad Mashhood Hassan | Dr. Jawaria Ali Khan | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The present project was carried out to diagnose the paramphistomum infection in sheep and to evaluate the efficacy of oxyclozanide and albendazole and to observe the effect of paramphistomum on various blood parameters, haemoglobin estimation, erythrocytes sedimentation rate and packed cell volume. For this purpose twenty four Sheep were selected and divided into 4 groups. Each group comprised of 6 animals.
Faecal samples were collected from Out Reach centre Sitar wala and its adjacent areas and out door clinic UVAS Lahore for the diagnosis of paramphistomum. The faecal and blood samples were collected on day zero pre-medication, and day 7 and 10 post-medication. The drug efficacy was checked at those animals which were positive for the paramphistomum. The animals in Group A were treated with Oxyclozanide (Zanil) at a dose rate of 15 mg/kg body weight. The animals in Group B were treated with Albendazole (Albensel) at a dose rate of 10 mg/kg body weight. The animals in Group C were kept as positive control (infected-untreated) and Group D were kept as negative control (uninfected-untreated).
Eggs per gram (EPG) of the faeces were noted on day 0 day before medication. The efficacy of these drugs was calculated on the basis of reduction of eggs per gram (EPG) of faeces after treatment. Zanil showed a progressive decrease in the faecal egg counts.
The efficacy of Oxyclozanide (Zanil) was 58.33% on day 7 and 83.33 % on day 10. The efficacy of Albendazole (Albensel) was 36.36% on day 7 and 56.36 % on day 10. It was observed that Oxyclozanide (Zanil) showed better efficacy than Albendazole.
Haematological parameters showed different results. A marked decrease in haemoglobin was observed after the study of infected sheep and the haemoglobin value was in normal range in healthy sheep on day zero. Packed cell volume was reduced on day zero in all infected groups, but was normal in the group of healthy sheep. After the treatment these values increased towards normal range in group A and B. The erythrocyte sedimentation rate was increased after 24 hours on day zero in all infected groups A, B, and C but was normal in the group D of healthy sheep.
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104.
Prevalence Of Dermatophytosis In Domestic Dogs And In-Vivo Evaluation Of Topical Anri-Fungal Agents
by Muhammad Mudassar Aziz | Dr. Asim Khalid Mahmood | Dr. khalid Saeed | Dr. Muhammad Hassan Saleem.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: The pet dog is a domesticated animal, belonging to the family Canidae and it comes within the order Carnivora. The given nomenclature is used for all varieties of pet dogs. Earlier, this pet animal was used for different purposes which include hunting, fighting and also different types of work had been taken from it. Dermatophytosis is the common skin infection in dogs especially in the young ones. A total of 300 samples were collected. The 36 samples were found positive and then divided into 3 groups. In group K, Ketoconazole was applied topically twice a week. Later on the efficacy of Ketoconazole was observed by collecting the samples on day 7, 14 and 21 after the commencement of treatment, which was 20%, 60% and 90% respectively. Similarly, Group L was treated with Lime sulphur and the efficacy of Limesulphur against M.canis infection, was found to be 60%, 80% and 100% on day 7, 14 and 21 respectively. Group M was treated with Miconazole. The efficacy of miconazole against M.canis was found to be 50%, 75% and 100% on day 7, 14 and 21 respectively. At the end, it was concluded that Lime sulphur was the best drug of choice among the three drugs used and the efficacy of ketoconazole was lesser than the others.
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105.
Efficacy Of Antifungal Agents Against Dermatophytosis In Cats
by Muhammad Irfan Saleem | Dr. Asim Khalid Mahmood | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The cat is commonly kept as pet all over the world. The dermatophyte Microsporum canis is the most common fungal agent of dogs and cats. In the presence study, the prevalence of Microsporum canis was recorded in pet cats in the area of district Lahore. The animals found positive with Microsporum canis were divided into three groups named, group A (griseofulvin), group B (ketoconazole) and group C (terbinafine). All the drugs were given orally and the efficacy of each drug was observed by collecting the samples on 15 days interval after the commencement of treatment until the mycological cure was achieved up till day 90th. Prevalence of M. canis infestation was 31%. The breed wise prevalence was highest in Persian cats as compared to Siamese and local breed and the prevalence were also higher in young ones as compared to old age cats. Later on the calculation of efficacy revealed that griseofulvin was the best drug among the three drugs used.
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106.
Study Of Incidence Rate And Chemotherapy Of Balantidiasis In Donkeys
by Adnan Khan | Prof. Dr. Muhammad Sarwar Khan | Dr. Asim Aslam | Dr. Muhammad Avais.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Equines have been serving the mankind for many purposes since as early as the human history. In equines parasitic infection is a major problem. Factors such as continuous exposure to parasites, climatic conditions, and lack of knowledge on the part of livestock farmers regarding parasitic transmission play an important role in sustaining parasitic diseases. Balantidium coli causes a protozoal disease known as Balantidiasis. It is normally found in large intestine of pigs (wild and domestic) and also explained in camels, primates including human, rodents, and equines but distinctly found in dogs. Balantidiasis is manifested by diarrhea, dysentery, severe colic pain, tenesmus, anorexia, nausea and vomiting. Balantidium coli may act as opportunistic pathogen in other infectious diseases. There is considerable information on B. coli in cattle and buffalo raising communities in the world. Investigations on Balantidium coli have not been conducted actively in the study area where equine rearing is one of the major occupations. This study will therefore is expected to serve as base line data for further work on this parasite.
The faecal samples of 400 donkeys irrespective of age and sex reared in and around Lahore were examined coprologically out of which 73 were found positive for Balantidium coli yielding the incidence rate of 18.25%. Chemotherapeutic trials of Secnidazole and Nigella sativa were performed on a group of 10 donkeys each (Group A & B) along with keeping 10 donkeys as positive & 10 donkeys as negative control (Group C & D). The animals of group A were treated with secnidazole (Dysen forte) at a dose rate of 10mg/kg orally for one day. The group A Showed the efficacy of 89.51% at day 14th (post treatment). The animals of group B were treated with Nigella sativa (Kalonji) at a dose rate of 200 mg/kg body weight orally for five days. This group showed the efficacy of 40.02% at day 14th after medication.
Similarly the effect of B. coli on various blood parameters (Hemoglobin and Packed cell volume) was studied in these animals. At day zero (pre-medication), the average Hb (g/dl) and PCV (%) values of groups A, B, C and D were 10.61 + 0.076, 10.61 + 0.13, 10.60 + 0.15, 11.01 + 0.068 and 31.47 + 0.27, 31.48 + 0.22, 31.47 + 0.24, 32.14 + 0.243 respectively. On day 14th after medication the average Hb (g/dl) and PCV (%) values of groups A, B, C and D were 10.79 + 0.094, 10.69 + 0.126, 10.60 + 0.15, 11.01 + 0.068 and 31.79 + 0.16, 31.64 + 0.13, 31.47 + 0.24, 32.14 + 0.243 respectively.
On day zero (pre-medication), the hemoglobin and pack cell volume values of groups A, B and C were below the normal (Hb = 11.0 - 11.6 g/dl, PCV (%) = 32 - 33) while of group D were approximately normal. On day 14th (post-medication), there was increase in Hb and PCV values of groups A and B. The Hb and PCV values of group C and D were not changed.
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107.
Studies On Cyanide Toxicity In Ruminants
by Muhammad Avais | Prof. Dr. Muhammad Sarwar Khan | Prof. Dr. Muhammad Arif Khan.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The present study was conducted with the objectives to: (a) determine the cyanogenic potential of various livestock fodder and grasses, (b) determine CN? content in blood of ruminants feeding cyanogenic plants, (c) develop a simple, reliable and inexpensive assay for the determination of CN? in blood, (d) evaluate the efficacy of various antidotes against CN? toxicity in a rabbit model, (e) find out the effect of CN? on hematological and biochemical profile, (f) study the postmortem and histopathological changes associated with CN? toxicity in various organs and (g) study alterations in tissue oxygenation and metabolic variables during acute CN? toxicity in pigs. For this purpose a total of 500 samples of various plants being used as fodder to livestock were collected from the field and analyzed for CN? content spectrophotometerically. To develop a simple picrate method for CN? estimation in blood, two goats were infused with KCN at 0.6mg/kg for 1 hours. Blood samples were collected at intervals and were subjected to the general method. Standard curve was developed using standard solutions of various concentrations of CN?. Additionally 6 rabbits were also given CN? orally for 40 days and blood samples were collected for CN? estimation. Later on, this method was successfully used to determine CN? levels from 500 blood samples of cattle, buffaloes, sheep and goats (n=125 each) feeding cyanogenic plants which were collected from the field. For antidotal studies forty two rabbits were randomly divided into seven groups viz. A, B, C, D, E, F and G each comprising of six animals. Rabbits in group A were given feed only and served as negative control, while the rabbits in group B received feed plus oral solution of potassium cyanide (KCN) and were positive control. Animals in group C were given feed, KCN and intraperitoneal (IP) injection of garlic extract. Rabbits in group D were treated with feed, KCN and IP injection of sodium thiosulfate (STS). Members in group E received feed, KCN and IP injection of both garlic extract and sodium nitrite (SNT). Animals in group F were treated with feed, KCN and IP injection of both STS and SNT whereas the rabbits in group G were given feed, KCN and hydroxocobalamin IP. The treatments were given to respective groups for a period of 40 days. At the end of 40 days, serum and fresh urine samples were drawn from each rabbit to study biochemical panel. Subsequently the rabbits were euthanized for postmortem and histopathological changes in various organs. For hematological and growth rate studies 12 rabbits were divided into two groups of six viz. A and B. Rabbits in group A were given feed only while members in group B were treated with feed and oral KCN at 3mg/kg for 40 days. The animals were weighed after every 10 days. Feed consumption rate, feed efficiency and weight gain for the members of each group were recorded. Blood samples were also collected for hematological studies at 0, 10, 20, 30 and 40 days. To study tissue oxygen and metabolic variables during acute CN? toxicity, 26 piglets were anesthetized. The non-invasive monitors were used to measured oxygen saturation, heart rate and rhythm and cerebral response to sedation. The invasive monitors were placed to measured beat to beat variability of the arterial blood pressure, pulmonary artery pressures. Mixed venous oxygen saturation (SvO2) and cardiac output were continuously monitored. Regional brain O2 (cerebral cortex) and skeletal muscle O2 saturation (rSO2) were also measured via sensors. After getting baseline metabolic and hemodynamic measurements that included arterial and venous blood gas analysis, lactates and cyanide levels, all pigs were started on an infusion of NaCN (0.55 mg/kg/hr). The infusion continued until the occurrence of sustained apnea (?3 minutes). A non-significant difference was found in the CN? content of Sorghum bicolor and S. sudanese, while a significant difference was observed between the CN? content of Jumbo grass (S. bicolor x S. Sudanese hybrid) and S. halepense. Jumbo grass and S. halepense were found to have significantly higher CN? concentrations than S. bicolor or S. Sudanese. Maize has significantly lower CN? content compared to S. bicolor, S. sudanese, Jumbo grass, or S. halepense. A gradual increase in CN? content with increasing height of plants was also observed. Highest CN? levels were observed at heights of 91-100 cm in sorghum varieties. At greater heights, a gradual decrease in CN? content was seen in all plant species, with the lowest levels at heights of 200 cm or above. No CN? was detected in maize at heights over 131 cm. In picrate method, the calibration curve was linear (R2=0.99) in the range of 0.3-120 mg CN?/L. In standard CN? solutions a color change in picrate paper from yellow to brown was observed at a concentration of 3 mg CN?/L and above. This method was sufficiently sensitive to quantify the low concentrations (0.3mg CN?/L) of CN? found in ruminant blood. In goats infused with KCN, the CN? concentration in blood was time-dependent and continued rising during infusion, gradually declining after infusion ceased after 1 h. Blood CN? levels showed a time-dependent increase in all experimental rabbits with the maximum concentration (1.34 mg/L) at day 40. The highest blood CN? concentrations were found in cattle, followed by goats and buffalo, with the lowest in sheep. When blood CN? levels of these species were compared statistically, a non-significant difference was observed. A non-significant difference was found between males and females. No relationship was observed between blood CN? levels and age of the animal for any species. Buffalo, cattle, sheep, and goats allowed to graze showed significantly higher blood CN? levels than animals kept in a stall feeding system. Animals grazing on jumbo grass were found to have significantly higher blood CN? levels than those fed S. bicolor or S. sudanese with a non-significant difference found between animals fed S. bicolor or S. sudanese. No CN? was detected in blood samples of animals fed maize. Hydroxocobalamine was found to be a significantly more effective CN? antidote than garlic, STS, SNT plus garlic extract, or SNT and STS, either alone or in combination. A combination of SNT and garlic extract was the second most effective CN? antidote. The efficacy of garlic alone was significantly higher than STS or SNT in combination with STS. The efficacy of combined SNT and STS was superior to STS alone in treating rabbits with CN? toxicity. No rabbits in any group demonstrated gross deviation from the normal organ structure. The activities of serum ALT, AST, ALP, and LDH enzymes, as well as serum bilirubin, were significantly increased in CN? treated rabbits compared to controls. Severe hepatocyte vacuolation and degeneration were present in liver of rabbits in the CN? treated group. Liver of rabbits in the control group showed normal morphological patterns. The concentrations of serum urea, uric acid and creatinine were significantly higher in CN? treated rabbits than in control group. Urinary thiocyanate levels were also significantly higher in the CN? group than in controls. Kidneys of rabbits in the CN? group demonstrated severe glomerular and tubular necrosis and congestion. Pyknotic nuclei were present in tubular epithelial cells, whereas a normal histological pattern was observed in kidneys of rabbits in control group. Serum T3 and T4 levels were significantly lower in the CN? group compared to controls. A non-significant difference in blood glucose levels was recorded between rabbits in control and CN? treated groups, and histological examination of pancreas revealed no microscopic lesions. No significant differences were observed in serum cholesterol levels of CN? and control group rabbits. The serum albumin and total protein concentration in CN? treated rabbits were significantly lower than in the control group. Heart of rabbits in both CN? and control group did not show histopathological changes under microscopic examination. The erythrocyte count, the hemoglobin concentration, packed cell volume and mean corpuscular hemoglobin were all found to be significantly lower in blood of CN? treated rabbits than control group. The mean corpuscular hemoglobin concentration was significantly higher in the CN? group rabbits than control group animals. On the other hand, the difference in leukocyte count, differential leukocyte count and platelets were non-significant. The difference in total and daily feed consumption between CN? and control group rabbits was non-significant, whereas the feed efficiency of rabbits in the control group was significantly higher than for rabbits in CN? fed group. The net weight gain of rabbits in the control group was significantly higher than in the CN? fed group. Cyanide infusion to pigs resulted in toxic levels of blood CN? accompanied by lactic acidosis. In addition, there was a progressive increase in cardiac output, Venous oxygen saturation, heart rate, elevation of central venous pressure and pulmonary artery blood pressure. Skeletal muscle rSO2 progressively and significantly decreased with increasing lactate and CN? levels. However, there was no significant change in brain rSO2.
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108.
Determinaton Of Multiple Drug Resistance Against Pasteurella - Multocida Isolates From Cattle And Buffalo In District Naseerabad, Balochistan
by Abdul Salam Baloch | Dr. Muhammad Hassan Saleem | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: Hemorrhagic Septicemia is one of the major problems from livestock point of view with high morbidity, mortality, loss of production and cost of treatment. The predisposing factors such as high temperature, high humidity and uncontrolled transportation of cattle and buffalo round the clock favor outbreak from time to time in the District Naseerabad, Balochistan. Forty samples were collected aseptically from Cattle and Buffalo and transported for further processing placing temperature 4 0C in to the Laboratory Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore.
Samples were cultured on blood agar for isolation and purification .These isolate were identified macroscopic, microscopic and biochemical profile characteristics test. The antibiotic sensitivity test of Ceftriaxone, Cephaloridine, Trimethoprim, Streptomycin, Cinoxacin, Colistin, Ciprofloxacin, Norfloxacin, Chloramphenical and Amoxycillin were determined for P. multocida isolates and also observed noticeable resistance against these antibiotics. The collected data was tabulated and analyzed statistically.
The aim and objective of present study was to determine multiple drug resistance against Pasteurella multocida so that proper antibiotic for the above said ailment with recommended dosage may be accomplished according to standard dosage.
Availability: Items available for loan: UVAS Library [Call number: 1333,T] (1).
109.
Sero Epidemiology Transmission Dynamics And Hematological Studies On Neospora Caninum In Dairy Buffaloes
by Amir Nasir | Prof.Dr.Muhammad Ashraf | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The current study was carried out in two districts of the Punjab province (pakistan),
viz. Lahore and Narowallocated;on the eastern part of the province bordering Indian Punjab
::.
and the first study assessing the epidemiology of N caninum in dairy buffalo in Pakistan.
Overall, the true sercprevalence of Neospora caninum in dairy buffalo (assessed by using
cELISA validated for buffalo by using IFAT as reference test) of the two districts was 39.4 %
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with no significant difference in the prevalence of N caninum antibodies between male
(29.5%) and female (41.5%) dairy buffalo. However, in the Lahore district alone, the
prevalence in the female (46.1 %) buffalo was significantly higher than in their male (23.8%)
counterparts. In contrast, in Narowal district, the prevalence in male (42.4%) buffalo was
higher than females (32.2%) but this difference was not statistically different. The other risk
factors evaluated in regards to the prevalence of N caninum in buffalo were similar overall in
both the districts; these included age-specific, season-specific, pregnancy-specific, parity-
specific, abortion-specific and dog contact-specific prevalence. The difference in sero-
prevalence was not significant amongst all the ages but was significant between the younger
" 3yrs.) and adult (> 3yrs.) age buffalo. The adult buffalo showed a higher prevalence of N
caninum antibodies compared with younger buffalo. Anyhow, the age group of buffalo >3-5
yrs. showed consistently the highest prevalence of N caninum antibodies overall and in both
the districts in the current study. The summer season was the one in which buffalo of the two
districts and overall showed the highest prevalence, followed by high prevalence in autumn; while
the lowest prevalence of N caninum antibodies was observed in buffalo sampled during the
winter. The prevalence in the higher parities buffalo was significantly higher than the lower parity
and pregnant heifers. The prevalence in pregnant dams was significantly higher than in non-
pregnant buffalo in the herds in different areas of the two districts.
Similarly, aborting buffalo in regular contact with dogs- at the dairy properties showed significantly
higher prevalence of N. caninum antibodies than non-aborting buffalo without the contact of dog.
The serological analysis-of dogs (using cELIS A) present at some dairy buffalo
'"
properties generally showed high prevalence of N. caninum; antibodies at the dairy properties
where the buffalo were in regular contact with dogs compared with farm premises and their
buffalo without any contact with dogs at the farm. This signifies the roleof the dogs in the
-
. transmission of N. caninum oocysts and infection to buffalo (intermediate host) as the isolates
of dog and bovine origin has been reported to be identical (Stenlund et al., 1997).
The analysis of milk samples from the dairy buffalo. also tested for serum antibodies
using iscom (immune stimulating complex) ELISA and showed a good level of agreement
(Kappa ratio=O.567) between the performance of two tests (iscom ELISA versus cELISA).
However, the cELIS A (VMRD, Pullman, W A) showed higher sero-positivity compared to
iscom ELISA (SV ANOV A, Uppsala, Sweden). Therefore, the use of either ELISA used on
milk for evaluating the prevalence of N. caninum antibodies in buffalo can give good results
. for lactating buffalo, but cELIS A appeared to have superior sensitivity in our study. The use
of iscom ELISA offers advantages regarding ease of sampling and repeated screening of
lactating herds but with little lower sensitivity than serum antibody cELISA.
The co-existence of N. caninum with Br. abortus, another important abortifacient
pathogen in buffalo, is quite significant as the probability of such infectious abortions is
increased in the susceptible herds resulting in more economic losses compared to those
caused by single pathogen. The co-existence of Br. abortus in N. caninum seropositive
buffalo in the current study was 13.2%, while the overall prevalence of Br. abortus antibodies
in buffalo of two districts was 12.2%. The prevalence trend of Bt: abortus was related to age,
~
though not exclusively; as prevalence continued to increase with age except in the age groups
of 6-7 yrs, and> 7 yrs. old buffalo. The higher prevalence in the adult age groups indicates
the association of Br. abortus with development of the reproductive system of the host
(buffalo). It also suggests the increase in the exposure of the buffalo to the pathogen with
increase.in age to some good extent. However, this prevalence was statistically not signific~
"0.
in all the age groups of buffalo. The prevalence of co-existence was highest (18.5%) in -,
buffalo >3-5 yrs. old followed by 18.2% in buffalo >7 yrs. age. Howe er, statistically, the
difference in the prevalence of co-existing antibodies against N caninum and Br. abortus in
buffalo was not significant.
Hematological analysis of the N caninum sere-positive and sero-negative dairy
buffalo for different haematological determinants, such as total leukocytic counts (TLC),
differential leukocytic counts (DLC), RBCs, haemoglobin, haematocrit, platelets and blood
plasma glucose level showed no difference in sero-positive and sero-negative two groups
particularly, regarding the effect of sero-positivity in buffalo. Albeit, some interactions such
as season x N caninum seropositivity showed significantly lower monocyte counts and high
blood glucose level in N caninum sere-positive buffalo compared with sero-negative buffalo.
Overall, in both groups no significant changes were observed in the seropositive buffalo
compare to sero-negative ones. Some interaction have independently affected certain
haematological parameters although, the animals were not seropositive. This suggests the
effect of some environmental factors and physiology states of the buffalo on its haematological
dynamics. These effects included the effect of lactation on the lymphocyte count in the lactating
and non-lactating buffalo, where lymphocyte counts were higher in non-lactating compared with
lactating buffalo most possibly due to the recruitment of lymphocytes in the udder for excretion in
milk. Similarly, blood haemoglobin was lower in the hot months and packed cell volume was
higher in the cooler months of the year in the buffalo.
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110.
Epidemiology, Serodiagnosis And Chemotherapy Of Anaplasmosis In Cattle
by Farhan Ahmad Atif | Prof. Dr. Muhammad Sarwar Khan | Prof. Dr..Muhammad Arif Khan.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2011Dissertation note: Anaplasmosis is globally distributed tick-borne disease of livestock with great
economic importance in cattle industry. The current project was designed to estimate the
prevalence of anaplasmosis, ticks and risk factors associated with seroprevalence of
Anaplasma marginale among cattle in Sargodha, Khushab and Rawalpindi districts,
Punjab, Pakistan. Moreover, haematological changes in A. marginale infected cattle and
efficacy of chemosterilization regimens were evaluated using locally available drugs for
the elimination of adult naturally infected carrier cattle.
A total of 1050 blood, serum and tick specimens were collected from randomly
selected small holders (n=90) and private livestock farms (n= 12) using multistage cluster
random sampling technique. A total of 30 union councils, 34 cattle farms (30 small
holders and 4 livestock farms) and 350 cattle were selected as primary, secondary and
elementary sampling units from each district. Sampling unit was indigenous and
crossbred cattle of both the sexes.
Microscopic examination of the Giemsa stained blood mears revealed an overall
prevalence of blood parasites as 21.14%. Anaplasma marginale was the highe t prevalent
(5.81 %) haernoparasite of cattle followed in order by Theileria sp. (5.14%) and Babesia
bigemina (4.76%), respectively. Crossbred cattle were more susceptible to TBDs as
compared to the indigenous cattle. Highest prevalence of TBDs was recorded in summer.
The prevalence of tick-transmitted diseases was higher in small holders (31.3%) than
private livestock farms (17.5%). Chi square analysis indicated a significant association
(P<0.05) among indigenous and crossbred cattle to selected TBDs. Wherea. non-
significant association between different age groups, seasons, sex and farm sizes were
revealed.
The overall seroprevalence of Anaplasma marginale in cattle using cELlSA was
31.05%. Seroprevalence was higher in crossbred cattle of more than 4 years of age and
there was a significant (P<O.OO I) association between different age groups and breed. The
seroprevalence was significantly (P<0.05) higher in summer season in Sargodha and
Khushab districts. Moreover, the seroprevalence was significantly higher in small holders
in all study districts.
The epidemiological data and relevant information regarding area, host and farm
management factors were collected on a questionnaire through interview from each dairy
farmer, attendant or manager from September, 2009 to August, 20 10. Multivariate
analysis of risk factors revealed that cattle of more than 4 year of age (OR=5.42), heavy
tick infested (OR =2.10), crossbred (OR = 1.59) cattle were significantly at higher risk for
seroprevalence to Anaplasma marginale. Presence of Rhipicephalus (Boophilus)
microplus (OR=3.70), use of ivermectin (OR=3.97), moderate interval of acaricide
frequency (OR= 16.50), stall feeding (OR=4.90) and use of unhygienic needles
(OR=24.00) were significantly associated with seroprevalence to Anaplasma marginale
in cattle (P<0.05). The Sargodha district was at higher risk (OR = 1.81) as compared to
Khushab and Rawalpindi.
The tick species identified from cattle were Hyalomma anatolicum anatolicum,
Rhipicephalus (Boophilus) microplus, Rhipicephalus sanguine us, Rhipicephalus
(Boophilus) annulatus and Haemaphysalis sp. The overall prevalence of tick infestation
among cattle was 54.76%. The highest prevalence (57.71%) of cattle tick infestation was tick infested sites in cattle followed by dewlap (92%), inner thighs (90%), neck & back (54%), tail (26%), ears (13%), around eyes (10%), flanks (4%) and legs (2%).
The haematological changes were studied at different levels of parasitaemia " 7%, >7-15% and> 15%) in Anaplasma marginale infected Sahiwal and crossbred cattle. There was a significant difference (P<O.OS) among total erythrocyte count (TEC), total leukocyte count (TLC), haemoglobin (Hb), packed cell volume (PCV), mean corpuscular
haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) at different levels of rickettsemia in both breeds. ignificant difference (P<O.OS) was noticed among RBCs, PCV and MCH blood parameters between Sahiwal and crossbred cattle.
A total of sixty Anaplasma marginale seropositive adult Sahiwal cattle were selected having their ages between 3-4 years ranging in weight from 246-341 kg. The animals were divided in four groups designated as OXY -group-I, E RO-group-II, IMC- group-III and control-group-IV, comprising IS animals each. The seropositive animals received oxytetracycline (22 mglkg IV once in a day for five days), enrofloxacin (S mglkg IV once in a day for five days) or imidocarb (S mglkg 1M twice, 7 days apart). Re ult of chemosterilization study indicated that oxytetracycline 13/1S (86.67%) and irnidocarb
dipropionate II/IS (73.33%) eliminated Anaplasma marginale infection in adult naturally infected carrier cattle on S6th day. The carrier clearance was confirmed by cELISA followed by subinoculation of blood in seronegative splenectomized calves.
It was concluded that TTBDs are widely distributed in Punjab, Pakistan. Host. management and area factors are involved with the seroprevalence of Anaplasma marginale in cattle. Haemolytic anaemia is the major haematological finding of Anaplasma marginale in cattle. Oxytetracycline is more effective and safe In chemosterilization of persistent Anaplasma marginale infection in cattle. There is a need for country wide epidemiological studies on ticks and TBDs using advanced serological
and molecular techniques. Moreover, the identification of the potential vector of anaplasmosis should be required for the effective prevention and control of anaplasmosis in Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 1368,T] (1).
111.
Seroepidemiology And Immunoprophylactic Trials Of Rabies In Dogs
by Uzma Farid Durrani | Dr. Muhammad Sarwar khan | Dr. Muhammad Arif khan | Factuly of veterinary science.
Material type: ; Format:
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Publisher: 2010Dissertation note: A field and an experimental study were carried out on pet and experimental stray dogs,
respectively. The objectives of these studies were to evaluate the rabies immunoprofile of dogs vaccinated with different rabies vaccines under field and experimental conditions and determine the relation between vaccine type, post vaccination intervals, health status, breed, age, gender and dewormers on rabies virus neutralizing antibody (RVNA) titer. Field study was carried out on 300 pet dogs including 1-108 months old males and females. Dogs were allocated to three groups; (I) regularly vaccinated (II) irregularly vaccinated (III) maternally immunized, each with 100 dogs bled at various stages. Experimental study was carried out on 16 adult, healthy stray dogs equally allocated to four groups; three groups for monovalent and polyvalent vaccine trials with Rabisin, Rabisyva-VP13 and Hexadog DHP-LR at day 0 and 21. Fourth group was negative control. Sample collection was carried out on day 0 and day 21 post vaccination followed by sampling at 30 days intervals till ten months, in each group. RVNA titers of the serum samples were determined by Rapid Florescent Focus Inhibition Test (RFFIT) at Rabies Lab., Kansas State University, USA. In field study 58% dogs regularly vaccinated with Rabisin, Hexadog DHP-LR and Rabisyva-VPl3 vaccines, were diagnosed with protective RV A titers while 33% dogs were suffering vaccine failure. Mean protective RVNA titers monovalent Rabisin, Rabisyva-VP13 vaccines were higher than polyvalent Hexadog DHP-LR vaccine without a significant difference. Among irregularly vaccinated dogs, only 25% dogs exhibited protective RVNA titers while 25% dogs were diagnosed with vaccine failure. 50% irregularly vaccinated dogs exhibited RVNA titer below minimum protective level. Maternal rabies immunity was minimum protective level. Maternal rabies immunity was diagnosed in 20% puppies with protective RVNA titers and 20% puppies were suffering immunity failure.
Remaining samples were either diagnosed with RVNA titers below minimum protective level or dcould not be assayed due to toxic effect on BHK cells. No relation was observed among RVNA titers and vaccine type, health status and gender in any field study group while a significant relation was observed among RVNA titers, breed, age and post immunization intervals with a significant difference in all groups.
In experimental study there was no case of vaccine failure in any group and all groups
exhibited high protective RVNA titers at the termination of study. Both the increasing and
decreasing trends were observed at various intervals of experimental study. Mean RVNA titers
Were 4.53±4.23, 3.48±3.40, 9.36±7.12 in Rabisin, Hexadog DHP-LR and Rabisyva-VP13
vaccinated groups, respectively. A relation was observed among RVNA titers, vaccines and post
vaccination time intervals with a significant difference. However health status, gender and use of imomodulatory levamisol were not found to be significantly affecting RVNA titers in any
group.
On the basis of the present study it is concluded that monovalent rabies vaccines
Rabisyva-VP13 and Rabisin generate higher RVNA titers as compared to polyvalent
Hexadog DHP-LR vaccine under the both field and experimental conditions however, a high
incidence rate of vaccine and maternal immunity failure is one of the factors that can be
responsible for high incidence of rabies especially in rabies endemic area. It is also
concluded that health status, gender and immunomodulatory effect of levamisol do not
significantly affect the rabies immunity however, there is the significant effect of age and
post immunization intervals on rabies immunity.
Availability: Items available for loan: UVAS Library [Call number: 1384,T] (1).
112.
Clinico-Epidemiological And Therapeutical Studies On Coccidiosis ( Isospora ) In Dogs In And Around Lahore;
by Awais Younas | Dr. Muhammad Sarwar Khan | Dr. Kamran | Dr. Muhammad Ijaz | Faculty of veterinary science.
Material type: ; Format:
print
Publisher: 2012Dissertation note: The present project was designed to find out the prevalence of coccidiosis (Isosporiosis), to
evaluate the comparative efficacy of Sulfadimethoxine and Furazolidone and to study the effect
of Coccidiosis on blood parameters.
For this purpose, the faecal samples of three hundred dogs were examined in the Medicine laboratory, University of Veterinary and Animal Sciences, Lahore by direct smear method and concentration technique. Out of three hundred dogs, forty nine were found positive for coccidiosis, showing the prevalence of 16.33%. For the chemotherapeutical trials, forty five naturally infected and fifteen non-infected were selected and divided randomly into four groups having fifteen dogs in each group wise A, B, C and D. Group A was treated with Sulfadimethoxine at the dose rate of 60 mg/kg (orally). Group B was treated with Furazolidone at the dose rate of 20 mg/kg (orally). Group C was kept as infected non-medicated control. Group D was kept as non-medicated and non-infected control.
The faecal samples of experimental dogs were examined for OPG (oocyst per
gram) counts on zero day (pre-medication), 3rd day, th day, and 14th day medication and percentage reduction of oocysts was' calculated. Sulfadimethoxine showed 66.69%,90.89% and 98.29% efficacy and Furazolidone showed 63.95%,
88.78% and 96.64% efficacy on 3rd, th and 14th day, respectively.
Haematological study showed that the values of haemoglobin, packed cell
volume and total erythrocyte count were lower in groups A, Band C as compared to non-infected control group. The values of total leucocyte count were higher in group A, B and C' as compared to non-infected group. As a result of treatment in group A and B, the values of haemoglobin, packed cell volume and total erythrocyte gradually increased while that of total leucocyte count gradually decrease on day 3rd ,7111 and 14th post-medication. It has been observed during the present study that sulfadimethoxine and Furazolidone showed their effect on blood parameters as a result of coccidiosis.
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113.
Chemotheraph Of Ancylostomiasis In Cats In Lahore.
by Awais Bokhari | Prof. Dr. Muhammad Sarwar Khan | Dr. Muhammad Hassan Saleem | Dr. Nisar.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 2012Dissertation note: It has been a long time since the affection of cats is with human beings. Cats have recorded as most adoptable pet of the modern world. Cats can be found anywhere in the world. Many diseases of cats are zoonotically important and they may cause zoonotic problem in human and mainly these pathogen are involved in enteric problems. The relationship of feline with human does provide many benefits to them but also act as a cause of zoonotic diseases.
A total of 300 fecal samples were collected from cats and were examined in the Clinical Medicine Laboratory, University of Veterinary and Animal Sciences, Lahore. The positive samples for Ancylostoma were 65 out of 300, indicating prevalence of Ancylostomiasis was 21.66%. For the chemotherapeutical trails 18 positive cats and 6 healthy cats were divided into four groups viz. A, B, C and D with 6 animals in each group. The cats of group A were treated with Pyrantel Pamoate @ 20mg/kg orally. The cats of Group B were treated with the Albendazole @ 50mg/kg orally. The cats of Group C were kept as positive control while the cats of Group D were kept as negative control.
The fecal samples were collected on day 0 pre-treatment and then on day 7th, 14th and 21st of post-treatment. The efficacy of the drugs was calculated on the basis of reduction of the eggs count from the fecal samples on the respective days. The efficacy of Pyrantel Pamoate on these days was 43.47%, 67.39% and 94.20% respectively, against Ancylostoma. The efficacy of Albendazole on these days was 30.77%, 60.44% and 86.81% respectively, against Ancylostoma. Thus it was recorded
that Pyrantel Pamoate was more effective against Ancylostoma than Albendazole as it caused more reduction of the egg count than albendazole.
Hematological study showed that the values of hemoglobin, packed cell volume and total erythrocyte count were lower in groups A, B and C as compared to non-infected control group. The values of total leukocyte count were higher in group A, B and C as compared to non-infected group. As a result of treatment in group A and B, the values of hemoglobin, packed cell volume and total erythrocyte gradually increased while that of total leukocyte count gradually decrease on day 7th, 14th and 21st post-medication. It has been observed during the present study that Pyrantel Pamoate and Albendazole showed their positive effect on blood parameters as a result of Ancylostomiasis with more efficacy of Pyrantel pamoate as compared to that of Albendazole.
Availability: Items available for loan: UVAS Library [Call number: 1391,T] (1).
114.
Prevalence And Chemotherapy Of Gastro-Intestinal Paradites In Deer In Punjab, Pakistan.
by Ruheeb Aslam | Dr. Jawaria Ali Khan | Dr. Muhammad Hassan Saleem | Dr. Nisar.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 2012Dissertation note: The parasitic load on deer affects them badly and eventually leads to death. In the present study a total of 100 feacal samples were collected from deer and were examined in the Clinical Medicine Laboratory, department of CMS, University of Veterinary and Animal Sciences, Lahore. Out of 100 samples thirty eight were positive and among these thirty eight, 12 deer were infected with cestodes, 6 with nematodes, 12 with trematodes, and 8 with the mixed infection of parasites. So, the prevalence of the cestodes, trematodes, nematode and mixed infection was 12%, 12%, 6% and 8% respectively. For the chemotherapeutic trails thirty positive animals and 10 healthy animals were divided into four groups A, B, C and D with 10 animals in each. The animals of group A was treated with albendazole @ 7.5mg/kg orally. Those of group B was treated with the ivermectin @ 1mg/ 50kg orally. The group C animals were kept as positive control and group D were kept as negative control.
The feacal samples were collected on the 0 day (pre-treatment), 7th, 14th and 21st day (post-treatment). The efficacy of the drugs were calculated on the basis of reduction of the eggs count from the feacal samples on the respective days. The efficacy of albendazole 42.59 %, 71.3% and 87.96% against cestodes, 33.33%, 60.3% and 82.88% against nematodes and 40.18%, 68.22% and 89.72% against trematodes at day 7, 14 and 21 respectively.. The efficacies of ivermectin were 42.59 %, 73.15% and 88.88% against cestodes, 51.78%, 76% and 91.07% against nematode while 42.85%, 64.28% and 85.71% against trematodes at day 7, 14 and 21 respectively.
It is thus concluded that the ivermectin is more effective drug than albendazole as it caused more reduction of the egg count than albendazole.
Availability: Items available for loan: UVAS Library [Call number: 1392,T] (1).
115.
Epidemiology And Prophylaxis Of Babesiosis In Felidae
by Syed Saleem Ahmad | Prof. Dr. Muhammad Sarwar Khan | Prof. Dr. Muhammad Arif Khan.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1425,T] (1).
116.
Prevalence And Chemotherapy Of Schistosomiasis In Cattle
by Mudassar Nazar | Prof. Dr. Muhammad Sarwar Khan | Dr. Syed Saleem Ahmad | Prof. Dr.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1433,T] (1).
117.
Molecular Diagnosis And Therapeutic Trials On Fasciolosis In Sheep And Goats
by Khalid Mehmood | Dr. Muhammad Ijaz | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Format:
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Publisher: 2012Dissertation note: The fasciolosis in sheep and goat is an important and infectious disease of ruminants characterized by dullness, weakness, lack of appetite, pallor and edema of mucosa and death of animal. The accurate diagnosis of Fasciolosis remained a difficult task for the field practitioners and the right choice of medicine.
For this purpose 600 sheep and goats was examined for fasciolosis. The fecal samples were collected directly from rectum while bile samples were collected from various private and public slaughter houses of districts Okara, Sahiwal and Lahore. Fasciola egg was identified by standard text while molecular conformation of Fasciola spp. was done by using PCR. For therapeutic trials, sixty animals (n=30 sheep; n=30 goats) positive for Fasciolosis was equally divided into A, B and C groups and each group comprising of 20 animals (n=10 sheep; n=10 goats). The animals of group A was given Zanil (Oxyclozanide) orally @ 1ml per 3kg body weight; the animals of group B was given Albendazole orally @ 1ml per 3kg body weight and the animals of group C was given the grounded powder of Neem leaves (Azadirachta indica) @ 5-6 teaspoon (25-30gm) orally. Efficacy of drugs was measured on the basis of disappearance of Fasciola eggs at 3, 7 and 14 day of post-medication. Data on the prevalence of fasciolosis was analyzed by Pearson's chi-square test while therapeutic trials data was analyzed with one way ANOVA using Statistical Package for Social Sciences (SPSS); P < 0.05 was considered significant.
To study fasciolosis in sheep and goats, samples were collected from Lahore, Okara and Sahiwal. The collected samples were processed at the Medicine laboratory at UVAS, Lahore and LPRI laboratories of Bahadurnagar, Okara. In this study first of all the fecal and bile samples were examined through microscope then these samples were processed by PCR test. No study was conducted previously on the molecular diagnosis of fasciolosis in sheep and goats in Pakistan, this is the first report for the molecular diagnosis of fasciolosis. In all F. hepatica positive samples approximate 300-bp fragment was generated on gel tested with DSJF/DSJ3 primers for sheep and goats. All positive samples by microscope were also positive by PCR, but out of the 92 samples positive (43 in sheep; 49 in goats) by PCR test, out of these 14 (7 in sheep; 7 in goats) were negative by microscopy. This study provides the base line data for the molecular diagnosis of fasciolosis in sheep and goats in Pakistan.
Overall prevalence of fasciolosis in sheep in both fecal and bile samples in various districts of Punjab was calculated. Out of 300 samples (150 fecal; 150 bile) of sheep, F. hepatica prevalence rate was 12% (36 samples) by microscopic examination while 14.33% (43 samples) by PCR test. On the basis of microscopic examinations districts wise prevalence rate was 11, 12 and 13% in Lahore, Okara and Sahiwal respectively and by PCR test prevalence rate was 13, 14 and 16% in Lahore, Okara and Sahiwal respectively. Prevalence of F. hepatica was more in Sahiwal than Okara and Lahore in sheep in both fecal and bile samples on the basis of microscopic examination and PCR.
Overall prevalence of fasciolosis was also calculated in goats on the basis of microscopic examination and PCR in both fecal and bile samples. Among 300 samples (150 fecal; 150 bile) of goats F. hepatica prevalence was 14% (42 samples) by microscopic examination while 16.33% (49 samples) by PCR. On the basis of microscopic examinations the prevalence was 13, 15 and 14% in Lahore, Okara and Sahiwal districts respectively while on basis of PCR test prevalence was 15, 18 and 16% in Lahore, Okara and Sahiwal districts respectively. Prevalence of F. hepatica was more in Okara than Sahiwal and Lahore in goats in both fecal and bile samples on the basis of microscopic examination and PCR.
The eggs per gram (EPG) values and efficacy of Oxyclozanide (Zanil), Albendazole and Neem leaves against fasciolosis in 30 positives sheep was recorded at 3, 7 and 14 days after the treatment. As group A was treated with oxyclozanide (Zanil), its efficacy was 50, 75 and 90% at 3rd, 7th and 14th day respectively after treatment. The efficacy of albendazole was 46, 65 and 81% at 3rd, 7th and 14th day respectively while the efficacy of Neem leaves was lowest that was 15, 30 and 42% at day 3rd, 7th and 14th day respectively. The efficacy of oxyclozanide and albendazole was significantly higher (p= 0.000) than Neem leaves. In sheep against the fasciolosis Oxyclozanide significant effect on EPG followed by albendazole and Neem leaves.
The EPG and efficacy of Oxyclozanide (Zanil), Albendazole and Neem leaves were determined in 30 goats against fasciolosis at 3, 7 and 14th day of post treatment. The efficacy of oxyclozanide was 57, 76 and 90% at 3, 7 and 14th day of post treatment, respectively. The efficacy of oxyclozanide was higher than Albendazole against the fasciolosis. The efficacy of albendazole was 55, 68 and 79% at day 3, 7 and 14th, respectively. Efficacy of Neem leaves against fasciolosis was lower as compared to other two drugs, it was 15, 23 and 40% at day 3, 7 and 14th, respectively. When the efficacy was compared at different days, Oxyclozanide reduced the EPG significantly, followed by Albendazole and Neem leaves.
From this study it was concluded that PCR is more reliable technique than microscopic examination for the diagnosis of fasciolosis in sheep and goats. Microscopic technique provides the base line data for the diagnosis of fasciolosis in sheep and goats but PCR provides help to conduct further molecular diagnosis on fasciolosis as well as others parasitic diseases. Secondly fasciolosis which is an important parasitic problem of ruminants is significantly prevalent in sheep and goats in Lahore, Okara and Sahiwal districts of Punjab. Oxyclozanide is the most effective drug while albendazole and neem leaves (Azadirachta indica) are relatively less effective against fasciolosis in sheep and goats.
Availability: Items available for loan: UVAS Library [Call number: 1434,T] (1).
118.
Clinico-Chemotherapeutic Study On The Occurrence Of Navel Ill In Cow Calves
by Safdar Abbas | Dr. Syed Saleem Ahmed | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Navel ill occurs commonly in young calves. Calves less than 1 week of age are most commonly affected by infections of navel because the umbilical stump usually takes 7-10 days to dry up. Infection of navel develops when pathogenic bacteria contaminated the wet umbilicus under the unhygienic environmental conditions. It may also cause the death of newborns calves due to bacterimia and septicemic shock. The unhygienic environmental conditions like parturition of cow in dirty pen, poor navel hygiene practices, inadequate colostrum feedings within 6 hours after parturition or when umbilical cord is cut too close to abdominal wall of calf, may predispose a calf to navel ill.
The present study was conducted in and around Kahna region of Lahore to find out the disease percentage of navel ill in newborn calves, and chemotherapeutical trials were conducted to see the comparative effect of topical, parenteral and topical with parenteral therapy. For this purpose, 400 newborn calves of 15 days or less than 15 days age were randomly selected from small dairy farms and small holder farmers of study area.
Among 400 calves, 197 calves were randomly selected from different small holder farmers of study area and 203 were selected from small dairy farms. Among 197 calves from different local areas of Kahna, the occurrence of disease percentage of navel ill was 46.19% and among 203 calves from small dairy farms of study area, the occurrence of disease percentage of navel ill was 12.80%. The overall occurrence of diseased percentage of navel ill from dairy farms and small holder farmers of study area was 29.25 %. The overall percentage of infection at dairy farms and local small holder farmers of study areas was 21.73%, 37.50%, and 14.28%, 22.22% and 14.10% among Friesian, Sahiwal, Jersy, Cholistani and Crossbred female calves respectively. The overall percentage of infection at dairy farms and local small holder farmers of study area was 22.22%, 88.23%, 16.66%, 33.33% and 40.98% among Friesian, Sahiwal, Jersy, Cholistani and Crossbred male calves respectively.
For chemotherapeutical trials, calves were divided into five groups. Calves of group A (n=10) were treated with topical application of Pyodine solution and Cicatrin powder, applied to navel cord topically twice a day for five days. The average percentage recovery of group A among males was 41% and among females was 46.5 %, but the overall recovery in group A was 43.75%. Similarly, when calves of group B (n=10) were treated with parenteral therapy of Moxin and Meloxicam for five days, then the average percentage recovery of group B among males was 41.47 % and among females was 48.08 %, but the average recovery among male and female calves of group B was 43.60 %. Calves of group C (n=10) were treated with topical application of Pyodine solution and Cicatrin powder plus parenteral therapy of Moxin and Melacam for five days. The average percentage recovery of group C among males was 76 % and among females was 90 %, but the average recovery among male and female calves of group C was 83 %.
Calves of group D (n=10) were kept as positive control to observe complications. They were of diseased conditions. Among these calves, three calves got maggot wounds; one died due to septicemia, two calves got small hernia and remaining calves showed no further complications except existing signs. Calves of group E (n=10, healthy calves) were kept as negative control. Among these healthy calves, three calves became thin and emaciated but all others remained healthy and showed no clinical signs of any disease.
Finally, the obtained data were statistically analyzed by using Chi Square method (software stata). In conclusion, the statistical analysis showed that occurrence of disease percentage of navel ill was more in male as compared to female calves. Similarly, the occurrence of diseased percentage of navel ill was high in local areas i.e. small holder farmers as compared to dairy farms of study area. The chemotherapeutic trials conducted among different groups of calves showed variable results. Topical treatment showed some better results than parenteral treatment, but when topical treatment was given with parenteral therapy, the calves showed maximum recovery. On the behalf of this study, it was concluded that the occurrence of disease percentage of navel ill is high in calves reared under unhygienic conditions. At dairy farms, hygienic conditions were better due to some good calf rearing practices as compared to local small holder farmers, that's why the occurrence of disease percentage of navel ill was high at local small holder farmers than that of dairy farms. Similarly, the results of chemotherapeutic trials showed that simple topical or parenteral therapy alone was not effective much as compared to when both topical and parenteral therapy was given at the same time.
Availability: Items available for loan: UVAS Library [Call number: 1436,T] (1).
119.
Comparative Study Of Different Suturing Techniques For Cystotomy Closure In Canines
by Imdadullah Khan | Prof. Dr. Muhammad Arif Khan | Dr. Ayesha Hassan | Pror. Dr.
Material type: ; Format:
print
Publisher: 2012Dissertation note: The cystotomy is an incision in to the wall of urinary bladder which is commonly indicated for the removal of urinary stones and for the excision of invading tumors. Cystotomy wound is closed by varieties of suturing patterns which commonly include simple appositional and inversion types of suturing patterns. Appositional patterns are used in single-layer while double-layer appositional pattern is also used. Inversion types of suturing patterns are commonly used in double layers. Single-layer appositional patterns are commonly used in small and young animals while double-layer inverting pattern is preferred to apply in large animals. Double-layer inversion pattern has been found to decrease the size of bladder lumen when the bladder wall is thick. Bladder mucosa in all cases is not penetrated because of the danger of the formation of bladder stones. In three-layer closure mucosa is sutured as a separate layer and is indicated commonly when there is bleeding. The present project is aimed to conduct a comparative study of appositional versus inversion type of suturing patterns and two-layer versus three-layer of bladder wall repair.
The study was conducted on 24 dogs divided into four groups comprising 6 dogs each. In 18 dogs experimental cystotomies were performed which were closed in following manners. Group-A: the cystotomy wound was closed in two-layer simple interrupted suturing pattern, group-B: cystotomy wound was closed in two layers by simple continuous and continuous lembert suturing pattern, group-C: the cystotomy wound was closed in three layers and group-D was the control group. These suturing techniques were evaluated for leakage with the help of contrast radiography, ultrasonography, renal function tests and physical signs. These techniques were also evaluated for their effect on residual urine volume with the help of ultrasound machine.
All the suturing techniques provided water tight closure when evaluated through radiography, ultrasonography, kidney function tests and physical examination. The two-layer inversion patterns and three-layer closure techniques were found to effect residual bladder volume but the effect of three-layer closure technique was more pronounced. No crystals/stones were observed on postmortem examination of the urinary bladder in each operated dog. A well demarcated stricture was noted in dogs whose bladders were sutured in three layers. It was thus concluded form the research that appositional patterns do not affect the residual bladder volume, the catgut can be penetrated through the mucosa without endangering for cystolithiasis and the two-layer appositional suturing pattern is as effective as three-layer closure of cystotomy wound for the control of hemorrhage from the bladder mucosa.
Availability: Items available for loan: UVAS Library [Call number: 1445,T] (1).
120.
Study On Different Closure Techniques Of Nephrotomy In Dogs
by Muhammad Imran | Prof. Dr. Muhammad Arif Khan | Dr. Ayesha Safdar Chaudary.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Kidney is a vital organ of body. It plays an important role in whole-body homeostasis, regulating acid-base balance, electrolyte concentrations, extracellular fluid volume, and regulation of blood pressure. Kidney performs these functions in coordination of various endocrine functions; these include renin, angiotensin II, aldosterone, antidiuretic hormone, and atrial natriuretic peptide. There are different problems of the kidney like calculi lodged in the renal pelvis and neoplasia of the kidney in which nephrotomy is indicated. The project was designed to find out the most suitable technique of closing nephrotomy incision. For this purpose three groups A, B and C of dogs were arranged containing eight animals in each group. In group A after performing nephrotomy, 2/0 absorbable suture was placed through the cortex in horizontal mattress fashion and renal capsule was closed with 4/0 absorbable suture in a simple continuous fashion while in group B nephrotomy incision was apposed by applying gentle digital pressure for five minute and incision in the renal capsule was closed with 3/0 synthetic absorble suture. Whereas, in group C cut edges was apposed through tissue adhesive glue (cyanoacrylate). Physical evaluation, Urine examination i.e urine colour, Complete blood count, blood urea Nitrogen, serum creatinine and excretory urography at different intervals was carried out to evaluate the efficacy of these three techniques.
The present project clearly indicated that suturless nephrotomy closure technique was found to be more suitable and compatible technique with excellent clinical superiority in terms of good weight gain, better hemostais, minimal post operative complication and maintaining kidney function.
Availability: Items available for loan: UVAS Library [Call number: 1447,T] (1).
121.
A Study On Different Placements (Onlay, Inlay & Sublay) Of Prolene Mesh For The Repair Of Incisional Hernia
by Aftab Hussain | Prof. Dr. Muhammad Arif Khan | Dr. Ayesha Safdar Chaudary.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1448,T] (1).
122.
Clinico-Biochemical Study After Partial And Complete Blockage Of Ureters In Dogs.
by Naveed Hussain | Prof. Dr. Muhammad Arif Khan | Prof. Dr. Muhammad Sarwae Khan.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Ureteral obstruction is one of the most common problems associated with urinary system. It imparts serious consequences on the system, if not treated sharply. The present study was performed in twenty dogs after producing experimental obstruction through ureteral ligations of variable nature.
The aim of the experiment was to identify the effects of variable ligations on obstructed and contralateral kidney with the help of blood parameters including hematology and serum analysis along with diagnostic imaging i.e. radiography and ultrasonography. It was noted that hematology was of least significance as diagnostic aid while serum analysis i.e. BUN and S/Cr were of some worth in early stages. As far as diagnostic imaging was concerned IV urography is considered as first line diagnosis in advanced cases revealing kidney size, parenchyma and hydronephrosis, while Ultrasonography is more sophisticated and advanced which is helpful in initial diagnosis and can study deep structures of the kidney.
Results revealed that in partial obstructions, in obstructed kidneys severity of hydronephrosis and kidney damage was less than complete obstructions. In complete ureteral obstruction, deterioration of total internal structures including medulla , cortex and renal pelvis was observed. Role of contralateral kidney was also evident during the experiment, which compensated the physiological changes due to obstruction and helped the patient in returning to normal life but structural damage of variable nature was also noted depending upon the severity of obstruction in the ureter.
It was observed during experiment that the revival of renal function is time dependent which is inversely related to duration of obstruction. If obstruction, mild or severe, is released earlier, recovery is quick and complete renal function restoration is possible in 24 to 48 hours of obstruction, depending upon its severity. But if delay occurs, it results in irreversible damage to the obstructed kidney and after three to six weeks no functional parenchyma is detectable in severe and mild obstructions respectively.
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123.
Prevalence And Therapeutic Studies On Diabetes Mellitus In Pet Dogs At Lahore.
by Salman Khalid | Prof. Dr. Muhammad Sarwar Khan | Dr. Muhammad Ijaz | Prof. Dr.
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Publisher: 2012Dissertation note: Diabetes mellitus is one of the most important maladies of endocrine glands in dog and cats. It is due to the insufficient production of insulin by the pancreas. The multi-factors such as obesity, feeding, exposure to toxic drugs etc. are involved for the onset of this chronic disease. The basic energy fuel of the body is glucose. In the absence of insulin the glucose level in the blood is increases which can be noticed as hyperglycemia (high blood glucose level). There are the three types of diabetes mellitus which includes insulin-dependent diabetes mellitus (type I or IDDM), noninsulin-dependent diabetes mellitus (type II or NIDDM) and secondary diabetes mellitus. The dogs play a vital role in our society.
The present study was conducted on diabetes mellitus to study its prevalence, its effect on various blood parameters and to evaluate the efficacy of insulin therapy in pet dogs in Lahore, Pakistan. The 500 blood samples were collected from the dogs of age ?5 years, presented at Pet centre of the University of Veterinary and Animal Sciences (UVAS) Lahore and various private clinics in Lahore, and was processed in Medicine Laboratory of UVAS, Lahore. The blood glucose level was measured with the aid of glucometer. Out of 500, 16 (n=5 male & n=11 females) were positive for diabetes mellitus. The 3.2% prevalence of diabetes mellitus was recorded in pet dogs in Lahore. The study concluded that the prevalence of diabetes mellitus in female dogs (68.75%) was significantly (p<0.05) higher than that of male dogs (31.25%). The results of this study showed that the prevalence of diabetes mellitus was higher in old age dogs (>9 years) as compared with other age groups. Diabetes mellitus was found to be more prevalent in winter season same as in human beings. The clinical signs like Polyuria (81.25%), Polydipsia (68.75%), Hyperglycemia (100%), Dehydration (43.75%), Anemia (56.25%) and cataract (18.75%) were commonly observed in diabetic dogs. In the present study the pet dog breed like Yorkshire terrier, Samoyed, Pomuranian and Cross bred were prone to have diabetes mellitus whereas German shepherd, Bull dogs, Pugs and Labrador retriever were at low risk for this malady.
In the present project, the haematological study was conducted on 28 dogs (n=14 diabetic and n=14 healthy dogs). The five ml blood sample were collected from each dog directly from the cephalic vein and were shifted into a sterilized plastic bottle coated with EDTA@1mg/ml and were further analyzed through haemtological analyzer. The results showed the decreased number of RBCs (4.63±0.42) and an increased in PCV values (62.91±4.18). The results also showed dehydration and anemia in diabetic dogs.
In the present study the 21 dogs (n= 14 diabetic dogs, n= 7 healthy dogs) were selected for therapeutic trials. The dogs were divided into further three groups; A, B and C. Each study group was comprised of 7 animals. Group A (comprised of diabetic dogs) was treated with Humulin-N (Insulin) @ 0.5 U/Kg, q12h, SC for 7 days. The group B (comprised of diabetic dogs) was kept as positive control while the healthy dogs were kept in group C for negative control. After the seven days treatment of group A, the blood samples were collected every 2 hr interval for 12 hrs for measuring the glucose value. The process was repeated once in a week for one month. The four blood glucose curves were made. Each blood glucose curve has its own nadir value. The time of glucose nadir value was indicated the peak action of insulin. The blood glucose curves were made to evaluate the effectiveness of insulin. The therapeutic results of this study concluded that the Humulin-N (insulin) was much effective for management of diabetic dogs.
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124.
Prevalence And Chemotherapy Of Theileriosis In Equines In And Around Lahore
by Muhammad Azhar | Dr. Muhammad Ijaz | Dr. Muhammad Hassan Saleem | Prof. Dr. Azhar.
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Publisher: 2012Dissertation note: Theileriosis is an infectious malady of equidae characterized by intermittent fever, loss of appetite, anemia, loss of weight, dyspnea, pale mucous membranes, jaundice and recumbency. Considering the significance and utilization of equines in our country and the substantial losses rendered by theileriosis, the present study was designed to study prevalence and chemotherapy of theileriosis in equines in and around Lahore.
For this purpose 300 equines (Horses=100; mules=100; donkeys=100) were examined for theileria infection.The blood was collected by ear tip puncture and from Jugular venipuncture. Then thin blood smear slide was prepared and dried up in air and stained with Geimsa's staining method and examined under microscope. Theileriaequiwere identified by their morphological characteristics as described by Rashid et al. (2009) and Guimaraeset al. (1997).Over all prevalence of Theileriaequiin equines was estimated as 58%. There was also a mixed infection of Theileriaequi and Babesiacaballiin some cases. Individually there was 54%, 64% and 56% prevalence of Theileriaequi found in horses, mules and donkeys respectively.
The effect of theileriosis on various blood parameters (Hb, ESR, TEC, TLC, DLC and PCV) was determined in 30 equines (Horses= 10; mules= 10; donkeys= 10). The remarkable decrease in hemoglobin, TEC, PCV and lymphocytes was observed. The value of ESR increased while there were no remarkable changes in TLC, monocytes, basophils, neutrophils and eosinophil.
For chemotherapy, 36 equines (Horses=12; mules=12; donkeys=12) positive for theileriosiswere divided into three groups (A, B, and C). Each group was further subdivided into three subgroups comprising of 4 animals in each subgroup. The animals of group A (Horses=4; mules=4; donkeys=4) were treated with Imidocarbdipropionate (Imizol®, ICI, Pakistan) @ 2 mg/kg BW I/M, and the efficacy of drug was found 91.7% in equines against theileriosis.The group B (Horses=4; mules=4; donkeys=4) was treated with Buparvaquone (Butalex®, ICI, Pakistan) @ 2 mg/kg BW I/M alone and was observed 66.7% effective. While the group C (Horses=4; mules=4; donkeys=4) was treated with Auk(Calotropisprocera)@ 0.3mg/kg BW, 8 doses at alternate days (local treatment), which was found 58.3% effective. The efficacy of drugs was measured on the basis of disappearance of clinical signs and blood smear examination at day 2, 4, 6 and 10 of post-medication. The efficacy of each drug was measured through recovery rate of the animals.
Finally, the data on prevalence of theileriosis was estimated by Pearson's chi-square test for significance whereas data on hematology was analyzed by Student's T-test using statistical software package STATA 9.1 (College Station T×77845, USA), P < 0.05 was considered significant.
Considering the significance and utilization of equine in our country and the substantial losses rendered by theileriosis, the present project was designed to check prevalence and chemotherapy of theileriosis in equines in and around Lahore. The results of this study will help farmers and veterinary practitioners in field.
Availability: Items available for loan: UVAS Library [Call number: 1487,T] (1).
125.
Prevalence And Chemotherapy Of Trichuriasis (Whi Worm) In Dogs.
by Muhammad Ramzan | Dr. Muhammad Hassan Saleem | Prof. DR. Muhammad Sarwar Khan.
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Publisher: 2012Dissertation note: In developing countries like Pakistan the trend of establishment of kennel clubs are increasing day by day. While the population of dogs increase the strong practice of vaccination and deworming increase as well. Parasitism is a major problem in our country. It causes great economic losses. The genus Trichiuris is one of the important nematode which affect the dogs. Therefore a study of genus Trichiuris was conducted in month of March to May, 2012. For this purpose 300 hundreds dogs of different breeds of dogs were selected for investigation of Trichiuris vulpis. Samples were collected and brought to the Clinical laboratory of Department of Clinical Medicine and Surgery in University of Veterinary and Animal Sciences Lahore. In laboratory positive samples for Trichiuris vulpis were recorded. The overall prevalence of Trichiuris vulpis was found to be 20% .The dogs positive for Trichiuris vulpis were divided into three groups A, B and C having 10 dogs in each group. Group A and B were treated with pyrantal pamoate and fenbendazole respectively. While the group C was positive control. The efficacy of both the drugs were measured by the decrease in egg per gram of faeces after medication. The egg counts were made at day zero, 7th and 14th.
The efficacy of pyrantal pamoat recorded on day 7th and 14th was 95.30 and 98.61% respectively while the efficacy of fenbendazole recorded on day 7th and 14th was 90.76 and 93.84% respectively. The obtained data was tabulated and analyzed by ANOVA.
Statistical analysis showed that there was significant (p<0.05) difference between the efficacy of payrental pamoate and fenbendazole after treatment of infected dogs at day 7th and 14th. This analysis showed that payrantal pamoate is significantly superior in its efficacy than fenbedazole.
The purpose of the study for prevalence and estimation of comparative efficacy of two drugs against Trichiuriasis in dogs was accomplished.
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126.
Therapeutic Studies Of Cryprosporiduim In Sheep In And Around Lahore, City
by Bushra Saleem | Dr. Jawaria Ali Khan | Prof. Dr. Muhammad Sarwar Khan.
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Publisher: 2012Dissertation note: Keeping in view the importance of sheep in global agricultural economy valued by humans for its multiple uses. The present study was conducted on cryptosporidiosis, and to evaluate the comparative efficacy of paromomycin and Kalvanji therapy in lambs in and around Lahore Pakistan. In this plan of research work 400 lambs, less than 1 month of age suspected for cryptosporidium infection on the basis of clinical signs were included. The fecal sample were collected from sheep presented at medicine outdoor hospital of University of Veterinary and animal sciences, various veterinary hospitals in and around Lahore and was processed in medicine laboratory UVAS ,Lahore for observing the oocyst of cryptosporidium by using Ziehl-neelsen technique. The prevalence was found to be 24.25%., The 40 sheep were divided into four groups=designated as A, B, C & D. Each group was comprised of 10 sheep. In group A the infected sheep was treated with paromomycin@ 100 mg/kg orally for 5 days, Group B infected sheep was treated with Kalvanji@1/2 tea spoon for 5 days. Group C infected sheep kept for positive control, whereas healthy sheep group D were kept in negative control. After the 5 days treatment The effect of drugs were checked, by reduction of oocyst, at 0, 7 and 14 day. Oocysts of cryptosporidium were counted by using Mc Master Technique. The therapeutic results of this study concluded that paromomycin was much effective for cryptosporidiosis. we also done hematological studies on 40 sheep(n=30 sheep with cryptosporidiosis and n=10 healthy sheep) was included to see the effect of drugs on various blood parameters i.e PCV,TLC, The 5 ml blood were collected from each lamb directly from jugular vein, and were shifted into a sterilized plastic bottle coated with EDTA@1mg/ml were further analyzed through hematological analyzer . In disease condition the TLC and PCV was increased. The results showed the decrease number of TLC and PCV. One way ANOVA was used to analyze the data.
The outcome of this study would helped to control cryptosporidiosis in lambs and reduce their substantial losses rendered by cryptosporidium. It was concluded that paromomycin is much effective drug against cryptosporidiosis, as compared to Kalvanji oil.
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127.
Comparative Evaluation Of Conventional Method And Staple Suture Technique For Intestinal Aanastomosis In Dogs
by Hafiz Kashif Shahzad | Prof. Dr. Muhammad Arif Khan | Dr. Aneela | Dr. Shehla Gul Bokhari.
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Publisher: 2013Dissertation note: Intestinal resection and anastomosis is frequently essential during abdominal surgeries. Indications for intestinal resection are malignancy, inflammation, stricture formation, injuries and ischemia. Several surgical situations necessitate the resection of intestinal segments and the formation of consistent anastomoses. Conventionally, a large diversity of suture materials has been used to make hand-sewn anastomoses. Surgical stapler was made for the first time at the beginning of 20th century but its use remained limited in gastrointestinal surgery. Currently, stapled anastomoses is an essential part of the majority of the gastrointestinal surgeries. The simple-interrupted, single-layer, approximating technique, in which absorbable monofilament suture material is used, is presently believed to be the technique of choice for end-to-end bowel anastomosis. This procedure is mostly used in gastrointestinal surgeries with comparatively lesser complications. Intestinal anastomosis with skin staples was started by military surgeons for the soldiers suffering from bowel trauma caused by gunfire. In this technique, stainless steel skin staples were used to create a seromuscular intestinal anastomoses. There are several types of staplers available in markets, e.g. circular ligators, clip appliers, endoscopic staplers etc. The circular stapler is a disposable anastomotic instrument that places a round, double staggered row of titanium staples connecting intervening tissues. It can generate an end-to-end, end-to-side, or side-to-side anastomosis. This round stapler is available with a variety of staple line diameters to be used according to need. In current years, there has been a large shift from hand-sewn method towards the use of intestinal stapler in gastrointestinal surgery. They present a possible reduction in operational timing and are easy to learn and use than for hand-sewn method. So, staplers are very popular in the trainee. This research project was tested on 12 mongrel dogs divided randomly into two different groups (A and B). Each group comprised of 6 dogs. Conventional hand-sewn method of end-to-end anastomosis was applied on group A and stapled anastomosis was made in the dogs of group B. Two surgical techniques were applied for end to end anastomosis of jejunum. In group-A conventional hand-sewn anastomosis technique was applied. In this method a piece of jejunum was removed and then anatomosed with simple interrupted, single-layer, approximating technique. The absorbable, monofilament suture material was applied manually. This is currently considered the method of choice for end-to-end intestinal anastomosis in small animal surgery. This technique is well described and has been used throughout the canine gastrointestinal tract with a relatively low complication rate. In group-B a circular mechanical stapler was used to construct anastomosis. In this method a piece of jejunum was removed and end-to-end anatomosis was made using a mechanical stapler. Comparison of both techniques was made on the basis of clinical parameters (temperature, pulse, respiration, vomiting, diarrhea and blood in feces), radiographic evaluation, exploratory laparotomy, change in lumen diameter and duration of surgical procedure. There is a significant difference in change in diameter within each group before and after surgery but there is no significant difference in change in diameter or stenosis formation between these two groups. There is a significant difference in the duration of operation; stapled group significantly requires an average of 10 minutes less than conventional hand-sewn method. Conclusively it was inferred that there is no significant difference in change in diameter between stapled group and hand sewn group but the anastomosis construction time is shorter in stapled group. Stapled anastomosis is safe and faster technique than hand-sewn method. The prevalence of low complication rate makes it all the more ideal for application in routine practices.
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128.
Prevalence And Chemotherapy Of Coccidioosis In Small Ruminants In Dera Ghazi Khan
by Ghazanfar Rashid | Dr. Muhammad Avais | Dr. Muhammad Ijaz | Prof. Dr.
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Publisher: 2013Dissertation note: Coccidiosis is one of the most economically important parasitic diseases of small ruminants and infection may cause subclinical production losses to sheep and goat industry.Coccidiosiscause a serious threat to livestock productivity, due topoor feed utilization, stunted growth, poor weight gain, associated morbidity, mortality, and cost of treatment and control measures. The disease is worldwide in distribution and is caused by many species of Eimeria which invade cells of intestinal epithelium. To treat and control coccidiosis in sheep and goats, numerous drugs were found to be effective including Amprolium, Toltrazuril, Decoquinate, Diclazuril and Sulphadimidine.
The present study was conducted to determine the prevalence of coccidiosis in Dera Ghazi Khan, for this 752 fecal samples (376 from sheep and 376 from goats) were collected and examined through direct smear method and salt flotation method. Overall prevalence of coccidialinfection was 48.67%, with prevalence of 52.92% for sheep and 44.41% for goats. The prevalence of coccidiosis in sheep was significantly higher (P<0.05) compared to goats. Eleven Eimeria species were identified 6 from sheepand 5 from goats. In sheep the common Eimeria species wereE. ovinoidalis(56.78%), E. ahsata(47.23%), E. parva(35.67%), E. intricate (30.15%), E. faurei(26.63%) andE. pallid (19.09%). In goats the prevalent species wereE. ninakohlyakimovae(68.86%), E. alijevi(59.88%), E. arloingi(53.29%), E. caprina(46.70%) andE. hirci(22.15%). Eimeria infection was significantly high (P<0.05) in female (54.57%; 310/568) compared to male (30.43%; 56/184).The prevalence of coccidiosis was significantly higher (P<0.05) in ?6 month as compared to above 1 year age.A significant difference was found in three feeding systems (i-e) in stall feeding, grazing and both (grazing + stall) feeding system.Coccidiosis was significantly high (P<0.05) in animals with poor and week body condition compared to medium, good and fat animals. A strong association (P<0.05) was observed between fecal score and prevalence of Eimeria infection. Peak prevalence was observed in month of August (68.08% and 60.63%) while low in October (38.29% and 30.85%) in sheep and goats, respectively.
In present study threeaniticoccidial drugs were used and the percent efficacy of Furazolidon, Sulphadimidine and Amproliun was 96.34, 95.40 and 98.08 percent, respectively at day 7. Whereas 98.58, 98.03 and 99.55 percent,respectively at day 21.It was concluded from the results that there was no significant difference (P>0.05) among the percentage efficacy of Furazolidone, Sulphadimidine and Amproliun used in these trials.
Hematological analysis was performed using Automated Hematological Analyzer. Hematological analysis showed a significant reduction (P<0.05) in total erythrocyte count (TEC) and hemoglobin (HB) content due tococcidiosis. Results show a significantincrease (P<0.05) in TLC, MCV and PCV of infected animals as compared to control negative. Mean lymphocytes, Monocytes, MCHC and MCH counts were also decreased in infected animals. Hematological values of treated animals become normal at day 21stafter treatment.
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129.
Prevalence And Chemotherapy Of Bovine Coccidiosis In River Region
by Muhammad Shoaib Jahanzaib | Dr. Muhammad Avais | Prof. Dr. Muhammad Sarwar Khan.
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Publisher: 2013Dissertation note: Bovine coccidiosis-is an infectious-disease affecting cattle and buffaloes resulting in great economic losses to the beef and dairy sector all over the world. Coccidiosis is an intestinal disease and usually associated with poor growth, bloody diarrhea and sometimes death of young animals. As bovine coccidiosis is commonly found in cattle and buffaloes, therefore it severely affects the production of milk and beef in Pakistan.
500 fecal samples-were collected (250 from-cattle and 250 from buffaloes) randomly to investigate the prevalence of coccidiosis in River Ravi Region Lahore. Overall prevalence of coccidiosis was recorded 57.2 % and 58.8 % in cattle and buffaloes, respectively. Slightly high prevalence was found in female animals. Highest prevalence (66.66% and 70.31%) was observed in animals below 6 months of age, while lowest (44.87% and 48.91%) prevalence was found in animals above 1 year of age in cattle and buffaloes, respectively. The study further revealed that highest prevalence occurs in August, followed by September and October while lowest prevalence was observed in July. The infection rate was a little high in crossbred (59.26 %) and Sahiwal cattle (57.89 %) as compare to imported breeds. Seven Eimeria species were identified from fecal-samples of cattle while Six species were identified from buffaloes, where E.bovis and E.zurnii were found most prevalent among them. Coccidiosis was prevalent significantly higher (P < 0.05)-in animals housed in confined-and dirt yard (72.13 % in cattle, 74.13 % in buffaloes) as compare to animals housed on paved (58.49 % in cattle, 61.22 % in buffaloes) and those having outdoor access. Coccidiosis was most prevalent in stall fed (71.64% and 73.68 %) as compare to grazing (28.58% and 31.91%) cattle and buffaloes. Highest (73.07 % and 72 %) prevalence was found in animals with Poor body condition (score-1), while animals with body condition score 3 (Good) were found with lowest prevalence (37.5% and 47.76%). Highest (82.35 % and 84.21 %) prevalence was found in diarrheic while lowest (36.19 % and 42.85%) prevalence was observed in animals with normal fecal score (1) both in cattle and buffaloes, respectively.
Efficacy of different chemotherapeutic agents against coccidiosis in buffalo calves was also determined in current study. Non-significant difference-(P > 0.05)-was observed-in percentage efficacy of all three anticoccidial drugs at day 28 onward, i.e. 100 % for Amprolium (Amprovil), 99 % for Furazolidone (Furasym) and 99 % for Trimethoprim+Sulphadiazine (Trisym). All the three drugs showed satisfactory results against coccidiosis in buffalo calves and no toxic effects were found during and after treatment.
The current study also determined the effects of coccidiosis on various blood parameters of buffalo calves. A significant reduction in Total Erythrocytic Count (TEC), Hemoglobin concentration, PCV, MCH, MCHC and statistically significant increase-(P < 0.05) in TLC, DLC (except Lymphocytes) and MCV was observed in infected animals. Also a statistically significant difference-(P<0.05)-was observed in mean hematological-values of infected not-treated and treated animals at day 28, where treated groups showed an improvement to the normal values.
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130.
Therapeutic Trials Of Trypanosomiasis In Equines Of Gujranwala And Sialkot District.
by Syed Manuchahar Ali | Dr. Muhammad IJaz | Prof. Dr. Muhammd Sarwar Khan.
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Publisher: 2013Dissertation note: Trypanosomiasis is an infectious malady of equines characterized by weight loss, anemia, progressive paresis, enlarged lymph nodes, petechiae of serous membranes, conjunctivitis, edema of the legs and lower part of the body, urticaria, and alopecia. Considering the significance and utilization of equines in our country and the substantial losses rendered by trypanosomiasis, the present study was designed to study prevalence and chemotherapy of trypanosomiasis in equines of Gujranwala and Sialkot districts.
For this purpose, 300 equines (horses=100; mules=100; donkeys=100) were examined for Trypanosoma infection. The blood was collected by ear-tip puncture and from Jugular venipuncture. Then thin blood smear slide was prepared and dried up in air and stained with Geimsa's staining method and examined under microscope. T. evansi was identified by their morphological characteristics as described by Smyth, (1996). Overall prevalence of T. evansi in equines was estimated as 13%. Individually there were 16%, 13% and 12% prevalence of T. evansi found in horses, mules and donkeys respectively.
The effect of trypanosomiasis on various blood parameters (Hb, ESR, TEC, TLC, DLC, and PCV) was determined in 30 equines (horses=10; mules=10; donkeys=10). The remarkable decrease in Hb, TEC, PCV, and lymphocytes were observed. The value of ESR increased, while there were no remarkable changes in TLC, monocytes, basophils, neutrophils, and eosinophils.
For chemotherapy, 36 equines (horses=12; mules=12; donkeys=12) positive for trypanosomiasis, were divided into three groups (A, B, and C). Each group was further subdivided into three subgroups, comprising of 4 animals in each subgroup. The animals of group A (horses=4; mules=4; donkeys=4) were treated with diaminazine aceturate (Fatrybanil®, Fatro, Pakistan) @ 3.5 mg/kg BW I/M, and the efficacy of drug was found 83.3% in equines against trypanosomiasis. The group B (horses=4; mules=4; donkeys=4) was treated with isometamedium chloride (Trypamidium Sumarin®, Merial, Pakistan) @ 0.25 mg/kg BW I/M alone, and was observed 91.6% effective. While the group C (horses=4; mules=4; donkeys=4) was treated with Auk (Calotropis procera) @ 0.3mg/kg BW, 8 doses at alternate days (local treatment), which was found 41.6% effective. The efficacy of drugs was measured on the basis of disappearance of clinical signs and recovery rate of the animals, and blood smear examination at day 2, 4, 6 and 10 of post-medication.
Finally, the data on prevalence of trypanosomiasis was estimated by Pearson's chi-square test for significance whereas data on haematology was analyzed by Student's T-test using statistical software package STATA 9.1 (College Station T×77845, USA), P < 0.05 was considered significant.
Considering the significance and utilization of equine in our country and the substantial losses rendered by trypanosomiasis, the present project was designed to check prevalence and chemotherapy of trypanosomiasis in equines of Gujranwala and Sialkot districts. The results of this study will help farmers and veterinary practitioners in field.
Availability: Items available for loan: UVAS Library [Call number: 1579,T] (1).
131.
In-Vetro And In-Vivo Anti-Theilerial Activity Of Medicianal Plants
by Mukhtar Ahmad | Prof. Dr. Muhammad Ashraf | Prof. Dr. Muhammad Sarwar Khan.
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Publisher: 2013Dissertation note: In vitro study was carried out in order to estimate the anti-theileria effect of Calotropis procera and Peganum harmala. Water and chloroform extracts of each plant were used in-vitro study along with standard drug Buparvaqoune (Butalex®). For this four concentrations i.e.4, 8, 12 and 16mg/ tested solutions of each extracts of each plant were applied on cultured lymphocytes exposed to theileria parasite infection. ELISA reader findings showed that the mean OD were found less in treated theileria infected lymphocytes cell culture as compared to untreated culture wells mean OD values. The highest cell reduction (94.36%) with C. procera chloroform extract treatment was observed at a concentration of 16 mg/ml solution. Lowest concentration (1mg/ml solution) of C. procera chloroform extract reduced non-significant (P>0.05) lymphocytes cell proliferation (40.97%) as compare to control negative group. P. harmala water extract was effective against the theileria parasite as significantly lower (P<0.05) mean OD value (1.802 ±0.341) was measured at a concentration of 4mg/ml solution and maximum inhibitory effect (92.20) was seen at a concentration of 16 mg/ml solution. ELISA reader findings showed that P. harmala chloroform extract treatment failed to inhibit lymphocytes cells propagation even at highest concentration. The highest inhibitory effect (85.33%) against theileria infected lymphocytes propagation was seen at a concentration of 16 mg/ml solution.
Plant extract was evaluated in respect of feed intake in rabbits. It showed that when administered extracts of C. procera in rabbits at dose of 3 mg and 5 mg/kg body weight, did not affect on feed intake in rabbits. However the chloroform and water extracts of both plant i.e. C. procera and P. harmala when were administered in rabbits parentally at dose 10 of mg/kg body. It showed that the feed intake of rabbits was non-significantly reduced as compared to other treatments groups. Hematological parameters such as WBC X103 count, RBC X 106 count and Hb g/dl values were measured at various days. Findings showed that significantly lesser RBC X 106 count was in group A3 and D3 than control at day 30 of experiment. A non-significant difference (P>0.05) was seen in RBC X 106 count and Hb g/dl measurements in all treatments groups. Kidney and liver functions were evaluated by measuring biochemical parameters, uric acid, creatinine and ALT at 0 days, 9 days and 30 days. Findings showed that serum creatinine and urea enzyme levels were significantly higher (P<0.05) in group A3 as compare to control group at day 30 of experiment. Serum level of urea was also significantly higher (P<0.05) in group B3 and D3 at day 30 of experiment. A non-significant difference (P>0.05) was seen in ALT in all treatment groups at day 30 than control. Post-mortem was performed at day 30 of experiment. Gross lesions consisting of hemorrhages, congestion, and lung emphysema were seen in rabbits treated with high dose i.e. 10 mg/kg of both extract of C. procera. Rabbits treated with P. harmala chloroform extract at dose 10 mg/kg showed moderate gross lesions. Histopathology of organs such as lungs, kidney, liver and heart was performed. Toxicity lesions were seen in rabbits treated with high dose i.e. 10 mg/kg of both extract of C. procera. Rabbits treated with P. harmala chloroform extract at dose 10 mg/kg showed histopathological lesions in lungs, liver and kidney.
Theileria infection was studied in vivo by developing through theileria infected Hayalomma ticks in crossbred calves (n=30) through. At day 15 of infection maximum increase in mean rectal temperature (105.24 ± 0.46F) was observed, twenty four calves had pyrexia (104.1- 105.6 F) and six claves were showing pyrexia > 105.6 F. A significant increase (P<0.05) in pre-scapular lymph node enlargement score of challenged calves was seen by day 7 of infection and maximum lymph node score (grossly enlarged size) was noticed in twenty calves (Table 4.14 , Plate 4.16) with peak mean score (2.73±0.44) on day 13 of infection. The piroplasm peak score (3.80±0.83) was observed in challenged calves at 22 day of infection and remained significantly higher (p<0.05) (2.60±0.54) in untreated calves until the 36 day of infection (Fig.4.29 and Table 4.16). A significant increase (P<0.05) in mean schizonts was observed in pre-scapular lymph node biopsy smear from day 7 of infection to onwards. Blood samples of challenged calves (n =30) were confirmed theileria positive through PCR test. The amplification of Theileria species were amplified at 1098 bp (Plate 4.20 and Theileria annulata was amplified at 721 bp (Plate 4.21).
In order to estimate the pattern of disease severity, severity score was measured by summation of mean score of piroplasms, schizonts, lymph node swelling and rectal temperature. From day 7, mild response (3-5 score) was seen in infected calves (n=10).
With increase in the severity of disease a significant decrease (P<0.05) was observed in mean values of the Hb g/dL amount, WBC and RBC count, Hct (%) concentration and lymphocytes percentage from day15 of infection onward to 36 day of infection. A non-significant decrease (P>0.05) in the mean values of MCH pg was seen throughout the experiment. A significant decrease (P<0.05) in mean values of MCHC g/dL along with significant increase (P<0.05) in the mean measurement of MCV fL (64.14±3.53) values was seen at day 36 of infection as compare to day 0 values, indicating macrocytic hypochromic anemia in challenged calves. These findings showed a significant increase (P<0.05) in excretory products (uric acid and creatinine) from day 15 of infection and onward as compared to day 0 values, indicating damaged kidney in infected calves. Biochemical analysis showed the significant increase (P<0.05) in liver enzymes (ALT, AST) from day 15 infection and onward.
Anti-Theileria activities of drugs were estimated by evaluating clinical manifestation of the disease and parasitological findings. Beside this treatment effect on hematological and biochemical reactions of liver and kidney functions was determined. A significant difference (P<0.05) in rectal temperature of calves groups (B and E) was observed than control positive (group F) at day 21 of post-treatment. On other hand calves treated with treatments A, C and D had a non-significant difference (P>0.05) in rectal temperature compared with untreated calves (group F). It was found that calves (n=5) dosed with C. procera chloroform extract (group A) had rectal temperature in normal range by the day 7 of post-treatment. Similarly calves (n=5) treated with Butalex were found with normal rectal temperature from the day 7 of pos-treatment. On other hand, at day 21 of treatment 40%, 20%, 40% and 80% calves were found with pyrexia in treatments groups B, C, D and F, respectively (Table 4. 46).
By the day 14 of treatment, calves of treatment groups B and E showed no parasitemia (piroplams ?1). Disease severity was estimated on accumulative score of rectal temperature, lymph node swelling and parasitological findings (piroplasms and schizonts score). It was found a significant decrease (P<0.05) occurred in the disease severity of score of disease in calves of groups B and E as compare to A, C and F at day 3 of post-treatment. At day 21 of treatment all treated calves were recovered from anemia.
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132.
Prevalence And Therapeutic Studies On Diabetes Mellitus In Cats In & Around Lahore
by Asma Rashid | Dr. Jawaria Ali Khan | Dr. Muhammad Ijaz | Dr. Shehla Gul.
Material type: ; Format:
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; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1592,T] (1).
133.
A Study On Ticks And Hemoprotozoan Infections In Camels (Camelus Dromedarious) In District Dera Ismail
by Naimat Ullah | Dr. Aneela Zameer Durrani | Dr. Muhammad | Dr. Muhammad Ijaz.
Material type: ; Format:
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; Nature of contents: ; Literary form: Publisher: 2013Dissertation note: Background
Pakistan is gifted with a large population of small ruminant including 58.3 and 27.4 million heads of goats and sheep respectively 2010.According to Livestock Census 2006, there were about 92164 sheep and 221912 goats' populations in Bannu district. Sheeps and goats are playing a leading role in the national economy by producing wool, skin, meat, milk and farm yard manure. Fasciolosis, parasitic gastrioenteritis, hydatidosis, coccidiosis, ectoparasitism, theleriosis and babesiosis are the major parasitic problems of small ruminants .There was an urgent need to carry out research on applied aspect leading control of parasites.
Study Objectives: The main objectives of the present study were;
(i) To have an overview on the overall prevalence of ectoparasites and endoparasites in small ruminants throughout the district by means of fecal tests.
(ii) To observe the affect of ecto and endoparasites on blood profile of the infected individuals.
Hypothesis: It was hypothesized that
¢ Parasitic diseases are highly prevalent in sheep and goat in District Bannu..
¢ External and internal parasites have significant effect on blood parameters.
Methodology and Results
During this study period 500 sheep and 500 goats were examined for endo and ectoparasites while using various fecal tests such as using direct microscopic examination, simple test tube flotation method and sedimentation method for endoparasites. Out of 500 goats and 500 sheep, 47 goats and 63 sheep were found positive for ectoparasites and percentage ratio was 9.4% in goats and 12.6% in sheep. Mostly, three types of ectoparasites were observed in both of cases i.e. goats and sheep (ticks, lice and mites). The total no. of positive cases found for ectoparasites in goats were 244 (ticks 10.2%, lice 30.4% and mites 8.2% in district Bannu). This result indicates a significant load of ectoparasites in this area of the country. Out of 149 positive cases of ecto-parasites in sheep; tick infection was 7.8% (39/500), lice 15.2% (76/500) and mite infection was 6.8% (34/500). Fecal samples of all 1000 animals were analyzed for the presence of endoparasites, out of which 90.6% goats and 87.4% sheep were found positive for endoparasites. Overall goats were more likely to be positive for Haemonchus Spp. and almost 50.2% (251/500) animals were found positive. The prevalence of other endo-parasites like Trichurus 5.6% (28/500), Strongyloid 4% (20/500), Coccidia 8.6% (43/500), Fasciola 12% (60/500) and Trichostrongyloide were 10.2% (51/500) while in case of sheep Haemonchus Spp. positivity was 33.2% (166/500). The prevalence of other Endo-parasites like Trichurus 6.8% (34/500), Strongyloid 2.4% (12/500), Coccidia 6.8% (34/500), Fasciola 10.6% (53/500) and Trichostrongyloide were 13.6% (68/500). The hematological profile of infected animals indicates high TLC and low hemoglobin level.
Conclusion
It is concluded that high prevalence was due to poor management and lack of knowledge about feeding, vaccination, hygienic conditions and lack of proper deworming programme. To overcome the problem it was necessary to educate the farmers about deworming programme and proper use of insecticides.
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134.
Identification Of Ticks And Tick-Borne Hemo-Parasitic Diseases Along With Therapeutic Trial Of Tick Infestation
by Sadaqat Ali | Dr. Muhammad Ijaz | Dr. Aneela Zameer Durrani | Prof. Dr. Azhar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1601,T] (1).
135.
Prevalence And Molecular Diagnosis Of Staphylococcus Aureus Subclinical Mastitis Innili Ravi Buffaloes At Livestock Experiment Station Bahadurnagar, Okara.
by Muhammad Altaf | Prof. Dr. Muhammad Sarwar Khan | Dr. Muhammad Avais | Prof. Dr.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1484,T] (1).
136.
Epidemiology Of Endo And Ecto Parasites Of Small Ruminants And Its Effect On Their Hematology Profile In District Bannu
by Niomat Ullah Khan | Prof. Dr. Muhammd Sarwar Khan | Prof. Dr. Muhammad Athar Khan.
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print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1607,T] (1).
137.
Comparative Efficacy Of Finney Pyloroplasty And Jaboulay Pyloroplasty As Method Of Treatment For Pyloric
by Mumtaz Ali Khan | Prof. Dr. Muhammad Arif Khan | Dr. Muhammad | Dr. Shehla Gul Bukhari.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1608,T] (1).
138.
Prevalence And Chemotherapy Of Anaplasmosis In Clinically Affected Small Ruminants Of Distric Lahore.
by Akhtar Ali | Dr. Muhammad Ijaz | Dr. Aneela Zameer Durrani | Prof. Dr. Azhar.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1610,T] (1).
139.
Srudy Of Mycotoxicosis In Cattle And Buffalo In District Sheikhupura, Punjab.
by Salman Arshad | Dr. Aneela Zameer Durrani | Dr. Muhammad Hassan Saleem | Ms. Sehrish.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1618,T] (1).
140.
Comparative Efficacy Of Herbal Preparations Of Leaves Of Neem (Azadirachta Indica) And Guava (Psidium Guajava) and Anticoccidial Drug on Coccidiosis in Pigeons (Columba livia)
by Abdul Qudoos | Dr. Syed Saleem Ahmad | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Format:
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drama
Publisher: 2013Dissertation note: The background of this study is that world health organization (WHO) reports that neurological disorders affect one billion people worldwide, including 50 million affected by epilepsy. Epilepsy is a common neurological disorder characterized by recurrent, periodic, spontaneous and unprovoked seizures. Childhood absence epilepsy (CAE) is an autosomal dominant disorder and a heterogeneous familial condition in which family members express absence seizures initially, and then show multiple phenotypes of myoclonic epilepsy including partial or absence seizures and generalized tonic conic seizures. Molecular genetics techniques have identified various CAE associated mutations in many genes i.e. ion channels (CACNA1H, CACNA1G, CACNA1N), sodium channel genes (SCN2A, SCN1A, and SCN1B) and some GABA receptor genes (GABRG2 and GABRD). CACNA1H ion channels are the principal intermediaries of fast neurotransmission in the CNS and have been frequently reported to play a significant role in a number of seizures. CACNA1H gene encodes the alpha (?) subunit and is usually located in post synaptic.
Various parameters are used in the present study was aimed to investigate coding regions of CACNA1H gene for analyzing the mutations involved in epilepsy. Blood samples of unrelated true representative of CAE were collected from psychiatry departments of different hospitals of Lahore. DNA were extracted with the standard protocol and amplifications of the CACNA1H regions were done with specially designed primers.
Later on, analysis of the results is done by sequencing of target fragments is carried out. Sequences are analyzed through BioEdit software and then aligned with the help of clustalW2 software.
It has been identified by the recent study on the absence epileptic patients of Pakistan that the gene CACNA1h has SNPs in the exon 9 and 10 at the position (2025G>A) and (4867 G>T) respectively which eventually alters the protein, making it hyperactive as the mutations are in the sensor regions of the protein, thus giving a 'gain in function' property to the ion channel.
In the conclusion, we must say that further study, with much larger sample number, is required to revise the effects of this polymorphism and accurately identifying the associated factors. There is a need to explore the other gene mutations causing epilepsy in local population of Punjab and Pakistan that will ultimately help to develop genetic counseling strategies, gene therapies and prenatal diagnostic procedures for the population of Pakistan.
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141.
A Study On Variable Degrees Of Angles In Z-Plasty Technique To Evaluate Extent Of Relaxation Of Contracted Skin
by Muhammad Asif | Prof. Dr. Muhammad Arif Khan | Dr. Muhammad | Dr. Shehla Gul Bukhari.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1648,T] (1).
142.
Identification Of Ticks And Tick Bornehemoparasitic Diseases In Equines Of District Lahore.
by Khadija Javed | Dr. Muhammad Ijaz | Dr. Aneela Zameer Durrani | Prof. Dr. Azhar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1651,T] (1).
143.
Prevalence And Molecular Genetic Characterization Of Different Isolates Of Hydatid Cyst In Ruminants In Okara
by Ali Abbas | Dr. Muhammad Avais | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Format:
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Publisher: 2013Dissertation note: Cystic hydatid disease has a worldwide distribution. Echinococcusis cause great economic loses in Pakistan and lead to the loss of worth 276.20$ per 100 sheep and goats, as well as 165.72$ per 100 infected buffaloes, cattle and camels. It has zoonotic importance and also have well-recognized zoonosis in Pakistan and numerous cases have been reported in the medical literature. Cystic hydatid disease is caused by Echinococcus granulosus, (E. granulosus), tapeworms. Adult tapeworms of this specie are small in size. Their measurement revealed that they reach upto 2-11 mm in length and contain protoscolex, the cephalic end for attchment and 2-5 proglottids segments. Two rows of keratinized hooks and four number of suckers are present on scolex. The last gravid proglottids segments bear the large number of fertilized eggs, which are shed after every 7-14 days. Eggs are oval in shape, having clearly distinct oncophore and 30-36 micrometer in diameter. There are several different strains of E. granulosus, which are genetically distinct. These strains vary phenotypically and therefore, this feature can be used for the control of this parasite. 10 genotypes of E. granulosus are identified up till now. Therefore, in this study we used PCR technique for prevalence determination and molecular characterization of specific strain of E. granulosus. The primers specific for E. granulosus were used in this study. The E.g.ss1for (5¡Ç-GTA TTT TGT AAA GTT GTT CTA-3¡Ç) worked as forward primer, while E.g.ss1rev (5¡Ç-CTA AAT CAC ATC ATC TTA CAA T-3¡Ç) worked as reverse primer. For this purpose, total 200 numbers of cyst samples (100 from each district) were collected from liver and lungs of cattle, buffaloes, goats and sheep slaughtered at different private and public abattoirs in Okara and Jhang. An antimortem examination was performed on each animal
LIV
and data regarding each animal entered in data capturing form before slaughter. Whole cyst sample was collected without rupturing and preserved in ice packs. Then these isolates were transferred to laboratory and stored at -20 ¢ªC for further processing. For the genetic analysis of E. granuous (Hydatid cyst) DNA was extracted from germinal layer and cystic fluid by using DNA extraction reagent (TRIREAGENT¢ç, Molecular Research Center, Ohio, USA) according to the manufacturer.s instructions. PCR was carried out by using primers specific for G1 strain of E. granulosus. The whole three steps (Denaturation, Anealing, Amplification) of PCR was carried out in PCR thermo cycler under conditions specific for E. granulosus. The final PCR product was electrophoresed in a 1% agarose gel containing 0.5 ug/ml of ethedium bromide. Electrophoresis was completed by applying 90 volts for 40 min. After required time, gel was placed in UV trans-illuminator to visualize the band shown by specific genotype of E. granulosus. Specific bands at 254 bp confirmed the G1 strain of E. granulosus. Results indicated the prevalence of 37% in Okara and 65% in Jhang district of Punjab. While in cattle (58.00%), buffalo (76.00%), sheep (36.00%) and goats (34.00%) prevalence was observed. As well as, (44.7%) prevalence of E. granulosus was calculated in liver and (54.8%) was observed in lungs in both districts. So, this study predicted the prevalence of hydatid cystic disease in ruminants in Okara and Jhang. PCR technique was used for the diagnosis of hydatid cyst in animals. This molecular characterization technique enables us to know the specific strain of E. granulous existing in these two districts. Finally the data gathered from this study help to understand the disease structure and to develop future plan.
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144.
Sero-Prevalence And Zoonotic Potential Of Toxoplasma Godnii In Camels In Cholistan Desert Area Of Bahawalpur.
by Hafiz Zia-ur-Rehman | Dr. Muhammad Avais | Dr | Dr. Jawaria Ali Khan.
Material type: ; Format:
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; Literary form:
drama
Publisher: 2013Dissertation note: Toxoplasmosis is a parasitic zoonosis with worldwide distribution caused by Toxoplasma gondii and is demonstrated in domestic livestock, wild life, other animals and humans. Keeping in view the zoonotic importance of the disease, the current study was conducted to find out the epidemiological status of toxoplasmosis in camels and human population in Cholistan desert area of Bahawalpur and to determine the possibility of transmission of toxoplasmosis from camels to their owners.
Serum samples from camels (n=226) and humans (n=113 camel owners and n=113 people having no contact with camels) were collected and analyzed by using Latex Agglutination Test (LAT) to determine the prevalence of Toxoplasma gondii in camels and humans.
Overall 9.73% camels were seropositive for anti-toxoplasma antibodies. In the month of April camels had the high prevalence of 11.5% (9 out of 78). . The highest prevalence (12%) was determined in the camels having age of 11 years or above. The prevalence of toxoplasmosis was high in the third trimester of pregnancy (27.2%). Although, the difference in prevalence of T. gondii infection was significantly different (P<0.05) at pregnancy of she camel. Overall 27 (12%) humans were seropositive for anti-toxoplasma antibodies. The highest prevalence of toxoplasmosis (15.25%) was detected in the people in 30-40 years or above age group. The highest sero-positivity was observed in camel owners (15.92%) and the lowest sero-positivity (7.96%) was observed in the people having no contact with camels. However, the difference in prevalence of T. gondii infection was significantly different (P<0.05) at the contact of humans with the camels.
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145.
Clinicao-Bacteriological Characterzation Of Mastitis In Cholistani Camel Breeds In Rahim-Yar-Khan, Pakistan.
by Noor-ul-Ain Sarwar | Dr. Aneela Zameer Durrain | Dr. Aftab | Dr. Muhammad Awais.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1696,T] (1).
146.
Comparative Efficacy Of Different Therapeutic Agents Against Pre In Goats
by Zunaira Akhter | Dr.Aneela Zameer Durrani | prof. Dr. Muhammad Sarwer Khan.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1703,T] (1).
147.
Comparative Efficacy Of Limited Contactdynamic Compression Plate (Lc-Dc) And Dynamic Compression Plate
by Farah Ijaz | Dr. Shehla Gul Bukhari | Prof. Dr. Muhammad Arif Khan.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1753,T] (1).
148.
Assessing The Efficacy Of Aqueous Garlic Extract Against Cyanide Toxicity In Mice Using Righting Reflex Recovery
by Sajid Ali | Dr. Muhammad Avais | Dr. Aneela Zameer Durrani | Prof. Dr.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: The KCN intake has been linked to central nervous system (CNS) syndromes and thyroid in animals as well as humans. It is very potent toxin even at very narrow dose ranges. Prolonged KCN exposure has also been associated with reduced growth rate in animals, disturbance in thyroid metabolism, lesions in liver, kidneys, lungs and also CNS pathology.
Male mice weighting about 20-40 grams were purchased from Veterinary Research Institute, Lahore, National Institute of Health, Islamabad, Animal house, University of Veterinary and Animal Sciences, Lahore and divided into 30 treatment groups. After acclimatization of mice, carefully, the mice were grouped as follows:
Control group
A group of 6 mice were treated with potassium cyanide (KCN) intra-peritoneally at 5.5mg/kg that induced a knock down state with recovery period of approximately 1 hour. Prior to each experiment, three or more mice were treated with KCN at this dose to verify that the recovery time was close to 1 hour.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) at 250 mg/kg: Group 1 (5-min pre-KCN)
A group of 6 mice was given AGE intraperitoneally (i.p) at 250 mg/kg. After 5 minutes of AGE injection, an i.p. injection of KCN at 5.5 mg/kg was given and righting reflex recovery time was recorded.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) at 250 mg/kg: Group 2 (5-min post-KCN)
A group of 6 mice was administered with KCN intraperitoneally (i.p) at 5.5 mg/kg. After 5 minutes of KCN injection, each mouse was treated with AGE at 250 mg/kg i.p. and righting reflex recovery time was noted.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) at 500 mg/kg: Group 3 (5-min pre-KCN)
A group of 6 mice was given AGE intraperitoneally (i.p) at 500 mg/kg BW. After 5 minutes of AGE injection, an i.p. injection of KCN at 5.5 mg/kg was given and righting reflex recovery time was recorded.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) at 500 mg/kg: Group 4 (5-min post-KCN)
A group of 6 mice was administered with KCN intraperitoneally (i.p) at 5.5 mg/kg. After 5 minutes of KCN injection, each mouse was treated with AGE at 500 mg/kg i.p. and righting reflex recovery time was recorded.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) at 750 mg/kg: Group 5 (5-min pre-KCN)
A group of 6 mice was treated with AGE intraperitoneally (i.p) at 750 mg/kg. After 5 minutes of AGE injection, an i.p. injection of KCN at 5.5 mg/kg was given and righting reflex recovery time was recorded.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) at 750 mg/kg: Group 6 (5-min post-KCN)
A group of 6 mice was administered with KCN intraperitoneally (i.p) at 5.5 mg/kg. After 5 minutes of KCN injection, each mouse was treated with AGE at 750 mg/kg i.p. and righting reflex recovery time was recorded.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) at 250 mg/kg: Group 7 (30-min post-KCN)
One more group of 6 mice was given AGE at 250 mg/kg orally. After 30 minutes of AGE administration, an intraperitoneal (i.p) injection of KCN at 5.5 mg/kg was given and righting refelx recovery time was noted.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) at 500 mg/kg: Group 8 (30-min post-KCN)
A group 6 mice was given AGE at 500 mg/kg orally. After 30 minutes of AGE administration, an intraperitoneal (i.p) injection of KCN at 5.5 mg/kg was given and righting reflex recovery time was noted.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) at 750 mg/kg: Group 9 (30-min post-KCN)
One more group of 6 mice was given AGE at 750 mg/kg orally. After 30 minutes of AGE administration, an intraperitoneal (i.p) injection of KCN at 5.5 mg/kg was given and righting reflex recovery time was recorded.
Garlic Extract Group: (Group 10-15)
Three groups 10 - 12 each with 6 mice were injected intraperitoneally (i.p) with AGE at 250, 500 and 750 mg/kg respectively and righting reflex recovery time of each mouse in respective dose group was noted to observe any effects of AGE on righting reflex recovery time. Three groups 13 - 15 each with 6 mice were treated with AGE orally at 250, 500 and 750 mg/kg respectively and righting reflex recovery time of each mouse in respective dose group was noted to observe any effects of AGE on righting reflex recovery time.
Potassium Cyanide (KCN) + Sodium Nitrite (SN): Group 16 (5-min pre-KCN)
A group of 6 mice was given SN at 20 mg/kg intraperitoneally (i.p). After 5 minutes of SN injection, an i.p. injection of KCN at 5.5 mg/kg was given and righting reflex recovery time was recorded.
Potassium Cyanide (KCN) + Sodium Nitrite (SN): Group 17 (5-min post-KCN)
Another group of 6 mice was administered with KCN intraperitoneally (i.p) at 5.5 mg/kg. After 5 minutes of KCN injection, each mouse was treated with SN at 20 mg/kg i.p. and righting reflex recovery time was noted.
Potassium Cyanide (KCN) + Sodium Thiosulphate (ST): Group 18 (5-min pre-KCN)
A group of 6 mice was given ST at 600 mg/kg intraperitoneally (i.p). After 5 minutes of ST injection, an i.p. injection of KCN at 5.5 mg/kg was given and righting reflex recovery time was recorded.
Potassium Cyanide (KCN) + Sodium Thiosulphate (ST): Group 19 (5-min post-KCN)
A group of 6 mice was administered with KCN intraperitoneally (i.p) at 5.5 mg/kg. After 5 minutes of KCN injection, each mouse was treated with ST at 600 mg/kg i.p. and righting reflex recovery time was recorded.
Potassium Cyanide (KCN) + Sodium Nitrite (SN) & Sodium Thiosulphate (ST): Group 20 (5-min pre-KCN)
A group of 6 mice was given SN and ST at 20 mg/kg and 600 mg/kg intraperitoneally (i.p) respectively. After 5 minutes SN and ST injection, an i.p. injection of KCN at 5.5 mg/kg was given and righting reflex recovery time was recorded.
Potassium Cyanide (KCN) + Sodium Nitrite (SN) & Sodium Thiosulphate (ST): Group 21 (5-min post-KCN)
A group of 6 mice was administered with KCN intraperitoneally (i.p) at 5.5 mg/kg. After 5 minutes of KCN injection, each mouse was treated with SN and ST at 20 mg/kg and 600 mg/kg i.p. respectively and righting reflex recovery time was noted.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) & Sodium Nitrite (SN): Group 22 (5-min pre-KCN)
A group of 6 mice was given AGE at 750 mg/kg and SN at 20mg/kg intraperitoneally (i.p). After 5 minutes of AGE and ST injection, an i.p. injection of KCN at 5.5 mg/kg was given and righting reflex recovery time was noted.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) & Sodium Nitrite (SN): Group 23 (5-min post-KCN)
A group of 6 mice was administered with KCN intraperitoneally (i.p) at 5.5 mg/kg. After 5 minutes of KCN injection, each mouse was treated with AGE at 750 mg/kg and SN at 20 mg/kg i.p. and righting reflex recovery time was recorded.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) & Sodium Thiosulphate (ST): Group 24 (5-min pre-KCN)
A group of 6 mice was given AGE at 750 mg/kg and ST at 600mg/kg intraperitoneally (i.p). After 5 minutes of AGE and ST injection, an i.p. injection of KCN at 5.5 mg/kg was given and righting reflex recovery time was recorded.
Potassium Cyanide (KCN) + Aqueous Garlic Extract (AGE) & Sodium Thiosulphate (ST): Group 25 (5-min post-KCN)
A group of 6 mice was administered with KCN intraperitoneally (i.p) at 5.5 mg/kg. After 5 minutes of KCN injection, each mouse will be treated with AGE at 750 mg/kg and ST at 600 mg/kg i.p. and righting reflex recovery time was noted.
Sodium Nitrite (SN) alone (Group 26)
6 mice in this group were treated with SN at 20 mg/kg intraperitoneally (i.p) and any effects on righting reflex recovery time were noted.
Sodium Thiosulphate (ST) alone (Group 27)
A group of 6 mice in group 27 were treated with ST at 600 mg/kg intraperitoneally (i.p) and any effects on righting reflex recovery time were noted.
Aqueous Garlic Extract (AGE) + Sodium Nitrite (SN) (Group 28)
The mice in group 28 were treated with AGE + SN at 750 mg/kg and 20 mg/kg intraperitoneally (i.p) respectively and righting reflex recovery time was noted.
Aqueous Garlic Extract (AGE) + Sodium Thiosulphate (ST) (Group 29)
A group of 6 mice in this group were treated with AGE at 750 mg/kg and ST at 600 mg/kg intraperitoneally (i.p) and righting reflex recovery time was recorded.
Sodium Nitrite (SN) + Sodium Thiosulphate (ST) (Group 30)
A group of 6 mice in group 30 were treated with SN and ST at 20 mg/kg and 600 mg/kg intraperitoneally (i.p) respectively and righting reflex recovery time was recorded.
The efficacy of the antidote estimated was based upon the reduction in righting reflex recovery time.
The righting reflex recovery time that was close to 1 hour was determined against KCN regimen. AGE was tested as potential antidote against sub-lethal KCN toxicity at dose regimens 250, 500 and 750 mg/kg intraperitoneally as well as orally at dose regimens 250, 500 and 750 mg/kg 5 minutes before and 5 minutes after KCN administration. SN and ST were tested at 20, and 600 mg/kg respectively as antidotes against sub-lethal KCN toxicity, 5 minutes before and 5 minutes after KCN administration. These were also tested in comparison and a dose with minimum recovery time was noted. A comparison was made between the efficacy of AGE and SN or ST or SN + ST as potential antidote against sub-lethal KCN toxicity. After 48 hours 3 mice from each group was sacrificed for postmortem examination.
For this purpose the righting reflex recovery time and survival data was collected for each mouse challenged with KCN. A group of 3 mice was tested with each increasing dose of KCN to create dose response curves for both righting reflex recovery times and percent survival. Increasing doses of KCN increased the recovery time of the righting reflex. The dose 5.5 mg/kg of KCN showed the righting reflex recocery time mean 64.66 ± .333 min and was selected for trials.
AGE (750 mg/kg) showed the most significant results as compared to ST and SN alone as well as in combination. Second most effective drug was ST as it showed better results than SN. Treatment results were more pronounced in 5 min pre-KCN groups as compared to 5 min post-KCN groups.
From this study it was concluded that the aqueous AGE is an effective antidote against sub-lethal KCN toxicity as the recovery times indicated that its effects are more pronounced than SN and ST, secondly, as it is easily available and very much cheaper in Pakistan and due to easy unavailability of SN and ST, it is effective antidote that could be used in the field against sub-lethal KCN toxicity with comparatively better results both orally and intraperitoneally.
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149.
Levels Of Maternal Antibodies Against Pasteurella Multocida In New Born Buffalo Calves
by Muhammad Bilal | Dr. Aneela Zameer Durrani | Dr. Muhammad | Dr. Muhammad Ijaz.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Buffalo is called black gold. The buffalo is raised for meat and milk purpose in Pakistan.The buffalo calves are at risk of many infectious diseases. One of the most important diseases is Hemorrhagic septicemia (HS). Hemorrhagic septicemia is caused by Pasturellamultocida important strains in Asia are B:2 and B:2.5. Buffalo calves are more suspected to the Hemorrhagic septicemia than adults one. In Pakistan the high prevalence of 49% is in the rainy season (Farooqet al. 2007)
Clinically the signs of Hemorrhagic septicemia includes profuse salivation,pyrexia, respiratory distress, swelling in throat, discharge through nostrils, protrusion of tongue, edema in the brisket area as well as in forelegs. Hemorrhagic septicemia can be diagnosed on the bases of clinical signs and laboratory conformation by Gram staining and. Serum agglutination test, Counter immune-electrophoresis and ELISA techniques are also used.Enrofloxacine was found to be effective treatment in case Hemorrhagic septicemia. Best protection measure vaccination by alum precipitated vaccine (Boudewijn et al. 2008).
During the present study blood samples were collected from the calves at different intervals of times. Serum was separated from that blood and was analyzed by using Indirect Haemagglutination(IHA) Test to observe the antibodies titer in the blood.The data was analyzed to calculate geometric mean titer (GMT) of the antibodies. Immunity status is much important in the defense of disease especially in the newly born claves which are more at risk of infections.The result of present study showed geometric mean titer 16 in colostrum of vaccinated dams and their newly born claves showed 0, 3. 4, 7 and 3 at 0 hour, 72 hour, 7, 15 and 30 days of their age. Colostrum of non-vaccinated dams showed GMT 8 and their newly born claves showed 0, 1. 3, 3 and 1 at 0 hour, 72 hour, 7, 15 and 30 days of their age.The maternal antibodies which are produce in the body of mother are transferred to their calves.The calves given birth by the vaccinated dams receive much higher level of antibodies from their mother than the calves born from non-vaccinated dams. This antibodies production in non-vaccinated dams is because of the carrier status of hemorrhagic septicemia.The non-vaccinated dams which are not expose to the any type of HS infection or not expose to the vaccines, they do not produce antibodies against Pasturellamultocidaand also not transfer to their young babies after birth.
The present study showed that claves of vaccinated dams are much protected in their early period of life against hemorrhagic septicemia. Buffalo calves need quick and intense care at early age and they need quick vaccinations.
The high maternal antibodies level against Pasturellamultocidain vaccinated dams then in non-vaccinated dams antibodies levels in newly born buffalo calves remain effective for longer period.
This study suggested for the vaccination of dams not only for the protection of themselves but also for the protection of their newly born claves at the crucial period of their young age.
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150.
Epidemiological Intelligence On Distribution & Dynamics Of Main Transboundary Diseases Of Ruminants In The Central Districts Of Punjab
by Muhammad Akram | Prof. Dr. Muhammad Athar Khan | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Format:
print
Publisher: 2007Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1844,T] (1).
