Dna Quantification Of Blood Stains Recovered From Washed Fabrics
By: Zeenat Salah-ud-din
| Dr. Muhammad Yasir Zahoor.
Contributor(s): Dr. Muhammad | Dr. Muhammad Wasim.
Material type: 
BookPublisher: 2013Subject(s): Department of Forensic ScienceDDC classification: 1663,T Dissertation note: Bloodstains are extremely important forensic evidences for DNA profiling. Bloodstains
recovered at crime scene can help to identify and confirm the credentials of the victim. Criminal
can make all efforts to destroy and hide possible evidence at crime scene therefore these
bloodstains on the crime place can hardly provide any evidence against the culprit. The criminal
will try to take away or change clothes with the bloodstains of the victim. Once perpetrator has
removed the bloodstains, culprit will feel secure and satisfied. But as a suspect if clothes are
recovered with washed bloodstains and forensic evidence of DNA of the victim can be recovered
from the washed clothes and can provide strong evidence against suspect. Present study has been
planned to quantiate DNA from 10 different types of bloodstained fabrics with possible ways of
washing to hide the blood from fabrics. 100 ìl of preservative blood was used in creating blood
spot on fabric. After that dried bloodstained fabrics were hand washed with tap water and in
detergent for 5 and 10mins and air dried at room temperature (25°C- 30°C) in laboratory. DNA
was extracted through Chelex® 100 extraction procedure from these fabrics and analyzed on
Real time PCR kit i.e. Quantifiler™ Human DNA Quantification kit which is specific for Human
DNA. Research study showed there was no statistically significant difference in DNA
concentrations among different washing methods (p-value = 0.318). There was statistically
significant difference in DNA concentrations among different types of fabrics divided into two
categories (p-value = 0.001, 0.002). Research findings showed that cellulose fibers recovered
highest quantity of DNA, followed by artificial fibers and then protein fibers (cellulose
>artificial>protein fiber) despite their washing methods. Thus provides a lead in solving such
type of criminal cases.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1663,T (Browse shelf) | Available | 1663,T |
Bloodstains are extremely important forensic evidences for DNA profiling. Bloodstains
recovered at crime scene can help to identify and confirm the credentials of the victim. Criminal
can make all efforts to destroy and hide possible evidence at crime scene therefore these
bloodstains on the crime place can hardly provide any evidence against the culprit. The criminal
will try to take away or change clothes with the bloodstains of the victim. Once perpetrator has
removed the bloodstains, culprit will feel secure and satisfied. But as a suspect if clothes are
recovered with washed bloodstains and forensic evidence of DNA of the victim can be recovered
from the washed clothes and can provide strong evidence against suspect. Present study has been
planned to quantiate DNA from 10 different types of bloodstained fabrics with possible ways of
washing to hide the blood from fabrics. 100 ìl of preservative blood was used in creating blood
spot on fabric. After that dried bloodstained fabrics were hand washed with tap water and in
detergent for 5 and 10mins and air dried at room temperature (25°C- 30°C) in laboratory. DNA
was extracted through Chelex® 100 extraction procedure from these fabrics and analyzed on
Real time PCR kit i.e. Quantifiler™ Human DNA Quantification kit which is specific for Human
DNA. Research study showed there was no statistically significant difference in DNA
concentrations among different washing methods (p-value = 0.318). There was statistically
significant difference in DNA concentrations among different types of fabrics divided into two
categories (p-value = 0.001, 0.002). Research findings showed that cellulose fibers recovered
highest quantity of DNA, followed by artificial fibers and then protein fibers (cellulose
>artificial>protein fiber) despite their washing methods. Thus provides a lead in solving such
type of criminal cases.
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