151.
Animal Adaptations: Evolution of Forms and Functions
by Saxena, R.K | Saxena, Sumitra.
Edition: 1st ed. Material type: ; Literary form:
not fiction
Publisher: India: MV Learning; 2015Availability: Items available for loan: Pattoki Library [Call number: 599.05 Saxena 31229 1st 2015 Zoology] (1).
152.
Statistical Methods / 8th ed.
by Snedecor, George W | Cochran, William G.
Edition: 8th ed. Material type: ; Literary form:
not fiction
Publisher: Delhi: Wiley Blackwell; 2014Availability: Items available for loan: UVAS Library [Call number: 519.5 Snedecor 31252 8th 2014 Statistics] (1).
153.
An Introduction to Genetic Engineering / 3rd ed
by Desmond, S. T. Nicholl.
Edition: 3rd edMaterial type: ; Literary form:
not fiction
Publisher: India: Cambridge University Press; 2010Availability: Items available for loan: Pattoki Library [Call number: 660.65 Nicholl 31650 3rd 2010 Genetics] (1).
154.
Molecular Biology of the Cell : The Problems book / 6th ed
by John Wilson | Tim Hunt.
Edition: 6thMaterial type: ; Literary form:
not fiction
Publisher: USA: Garland Science; 2015Availability: Items available for loan: Pattoki Library [Call number: 571.6 Wilson 31671 6th 2015 Genetics] (1).
155.
Genetic Polymorphism Of Prss12 Gene Responsible For Cognitive Dysfunction And Its Homology Analysis With Canine
by Hafsa Amjad (2014-VA-776) | Dr. Muhammad Yasir Zahoor | Dr. Muhammad Imran | Mr. Shahid Abass.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Neurotrypsin a multi domain serine protease predominantly expressed in brain is considered to be involved in cognition by the establishment and maintenance of synapses in mammals. Mutations in PRSS12 gene have been reported for cognitive disability in Algerian family.
In present study, DNA of 10 enrolled non-relative cognitive dysfunctioned patients was extracted through organic method. The normal individual samples of siblings and parents of relevant families was also included in this study as control. This amplification exon 7 of PRSS12 was done after designing primer by using Primer3 software. Exons was sequenced by using BigDye Terminator Cycle Sequencing Ready Kit(Perkin Elmer/ABI) and read in automated sequener, ABI Prism model 3730 (Perkin Elmer). No significant mutation was identified in affected individuals.
Computational comparative sequence analysis tools were used for the nucleotide and amino acid sequences to predict the homology in PRSS12 gene among mammals of well-developed cognition. PROSITE domain database search was performed to determine domain organization and Phyre software was used to develop secondary structural features and 3D protein models and ReptroX for multiple sequence alignment of tertiary structures. Using the generated alignments highly conserved regions in primary and secondary structures of neurotrypsin in mammals were identified. Phylogenetic analysis indicated highest similarity of human PRSS12 with non-human primates (chimpanzee, orangutan and monkey) followed by Catecians, Felis, and Canine evolving from the same ancestor. The predicted domain architecture shows the neurotrypsin consisting of kringle domain, four scavenger receptor cysteine-rich
CHAPTER 6
SUMMARY
Summary
68
domains and a serine protease domain named trypsin. Whereas mouse consists of only three scavenger receptor cysteine-rich domain. Prediction and comparison of domains in mammals indicated that primates and catecians protein domains have high similarity with humans. Computational analysis by using animal models can aid in evolutionary studies and. understanding the role of neurotrypsin in cognition. Availability: Items available for loan: UVAS Library [Call number: 2498-T] (1).
156.
Nucleotide Sequence Variation In Heat Shock Protein 70-1 Gene Of Capra Aegagrus Blythi
by Fehmeeda Fatima (2014-VA-775) | Dr. Asif Nadeem | Dr. Maryam Javed | Dr. Abu Saeed Hashmi.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Heat shock protein 70 (HSP 70) plays a vital role in survival of an organism by providing
cytoprotection against various kinds of stresses. Among all the HSPs present in the cell, the
ubiquitous HSP 70 proteins are the most abundant and temperature sensitive. Considering the
importance of HSP70-1 gene in conferring thermotolerance, present study has been designed to
characterize this gene in Sindh ibex which is a wild goat species of Pakistan. The
characterization of HSP70 gene might be helpful for deriving phylogenetic relationship among
different species and identifying new functions among the related species. Blood/meat samples
(n=25) were collected from Kirthar national park, Sindh. Standard DNA extraction method was
used for DNA extraction. PCR primers were designed by Primer3 software and amplification of
gene was done by Polymerase Chain Reaction. PCR product was sequenced bi-directionally by
Big DyeTM Terminator on ABI 3130XL Genetic Analyzer. Multiple sequence alignment was
performed for polymorphism identification. Genetic diversity was calculated by using DnaSP
v.5.0. Phylogenetic analysis using the MEGA v.6.0 software package was performed and
neighbor joining and UPGM trees were constructed. The results indicated that Sindh ibex
HSP70.1 gene was highly similar to of domestic goat, sheep, cattle, buffalo, camel and horse
which indicates their origin from a common ancestor. The results of this data might be helpful in
designing effective conservation strategies for Sindh ibex. Availability: Items available for loan: UVAS Library [Call number: 2524-T] (1).
157.
Development Of Dna Based Diagnosis Of Theileriosis In Cattle And Its Specificity With Blood Smear Microscopy
by Uzma Sarwar (2014-VA-777) | Dr. Wasim Shehzad | Dr. M. Yasir Zahoor | Dr. Ali Ahmed Sheikh.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Theileria annulata and Theileria parva are intra-erythrocytic parasites which are responsible for
causing tropical theileriosis and East Coast fever in cattle respectively. This parasite is
transmitted by ticks to vertebrate host i.e. cattle. Currently used diagnostic methods for diagnosis
of bovine theileriosis are clinical symptoms, peripheral blood smear microscopy and serological
tests (IFAT and ELISA).
Current study was conducted to compare the specificity and sensitivity of blood smear
microscopy and PCR techniques to diagnose bovine theileriosis. This study is comparative as
well as developmental in nature. Although peripheral blood smears microscopy is cost effective
and quick method of diagnosis in case of high or moderate parasitaemia in blood. But the
limitations associated with microscopy include false negative diagnosis in case of low
parasitaemia in chronic and asymptomatic infection, morphological similarity of Theileria with
other species of Plasmodium and Babesia. These limitations may lead to misdiagnose the
infection due to which disease may remain unnoticed. PCR based method, developed in this
study, and is found to be more specific and sensitive than conventional microscopy. Fifty blood
samples were collected from September, 2015 to November, 2015. These samples were screened
microscopically as well as with PCR for presence of Theileria. Nine samples were found to be
positive microscopically but 18 samples were found positive by PCR. The results obtained from
the study clearly show that PCR is more reliable, precise and sensitive assay for diagnosis of
bovine theileriosis then microscopy. It is hoped that proposed method to diagnose Theileria will
help to nullify the problems associated with microscopy. This will ultimately facilitate in the
formulation of effective treatment control and vaccine development strategies. Availability: Items available for loan: UVAS Library [Call number: 2547-T] (1).
158.
Molecular Exploration Of Zbed6 Gene For Growth Trait In Lohi Sheep
by Usman Sagheer (2014-VA-03) | Dr. Maryam Javed | Dr. Akhtar Ali | Prof. Dr. Tahir Yaqub.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: ZBED6 gene is a central transcription factor. It is as a repressor of IGF2 (insulin-like growth factor II) interpretation in skeletal muscle myogenesis and development. It is essentially included in organism development, signaling, cell to cell collaboration, hepatic fibrosis, clathrin intervened endocytosis and tight intersection signaling falls. Chromatin immune precipitation (ChIP) sequencing utilizing C2C12 cells recognized around 2,500 ZBED6 binding locations in the genome, and the derived accord theme gave an immaculate match with the set up tying site in IGF2. Silencing of ZBED6 in myoblast cells influences IGF2 expression, wound healing, cell proliferation and myotube arrangement. Genes connected with ZBED6 binding sites demonstrated a very huge advancement for certain Gene Ontology groupings, including improvement and transcriptional regulation.
Forty two blood samples were collected. DNA extraction was done by using organic extraction method. Primers for PCR amplification designed using Primer3 software. PCR products were sequenced and then analyzed by using BioEdit software. Expasy translational tool for translation and POPGENE 32 software for analysis of population genetics at all the loci were used. Using this software the overall allele frequency, heterozygosity, probability using Chi-square test and Likelihood ratio test and Hardy-Weinberg equilibrium, genotype distribution at all SNP position, summary of genetic variation statistics for all loci and association were calculated. After this, for the association one way ANOVA was performed. Single nucleotide polymorphism within ZBED6 could be potential candidate gene to be serving as genetic marker for the selection of animals with higher tendencies towards weight gain. Availability: Items available for loan: UVAS Library [Call number: 2539-T] (1).
159.
Molecular Exploration Of Zinc Finger Bed-Type Containing 6 Gene For Growth Trait In Beetal Goat
by Kanwal Rashid (2014-VA-496) | Dr. Maryam Javed | Dr. Asif Nadeem | Dr. Abu Saeed Hashmi.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Zinc finger, BED-type containing 6 (ZBED6), is a novel transcription factor.It acts as a repressor of IGF2 transcription in skeletal muscle myogenesis and development. it is mainly involved in organism development, signaling, cell to cell interaction, hepatic fibrosis, clathrin mediated endocytosis and tight junction signaling cascades. Chromatin immunoprecipitation (ChIP) sequencing using C2C12 cells identified about 2,500 ZBED6 binding sites in the genome, and the deduced consensus motif gave a perfect match with the established binding site in Igf2. Silencing of Zbed6 in myoblast cells affect Igf2 expression, cell proliferation, wound healing, and myotube formation. Genes associated with ZBED6 binding sites showed a highly significant enrichment for certain Gene Ontology classifications, including development and transcriptional regulation.Functional and signaling assays of BED6 gene indicate its probable role in controlling growth traits in Goat. Blood samples (n = 40) were collected. Inorganic method of DNA extraction used. Primers for PCR amplification will be designed using Primer3 software. PCR products will be sequenced bi-directionally on ABI 3130XL Genetic analyzer. The results of sequencing were analyzed using CHROMAS software. Sequence alignment tools (blast 2)were used for SNPs identification. Difference between allele and genotype frequency of studied gene evaluated by chi square test, likelihood test and analysis was done by POPGENE and one way ANOVA.Novel Variations identified which have probable implementation in selection of superior goats with higher tendencies towards weight gain. Availability: Items available for loan: UVAS Library [Call number: 2554-T] (1).
160.
Molecular Phylogeny And Diversity Analysis Of Bovidae (Boselaphus Tragocamelus, Antilope Cervicapra) And Cervidae (Axis Axis, Axis Porcinus) In Pakistan
by Ghulam Abbas (2011-VA-748) | Dr. Asif Nadeem | Prof. Dr. Mansoor Ellahi Babar | Dr. Muhammad Tayyab.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Many species of mammals have declined within the past two centuries due to human
caused disturbances and the unsustainable use of natural resources. Molecular methods have an
important role in phylogeny and diversity analysis. The present study was designed for diversity
analysis of Boselaphus tragocamelus & Antelope cervicapra (Bovidae) and Axis axis & Axis
porcinus (Cervidae) family in Pakistan. A total of 25 samples from each of the four species were
collected from different parks, zoos and natural habitats. DNA was extracted, PCR primers were
designed and cytochrome-b, cytochrome-c gene and d-loop regions were amplified by PCR.
PCR products were sequenced bi-directionally by Big DyeTM Terminator. Bioinformatics tools,
Blast 2 sequences, Clustal-W, MEGA-6, Bioconductor in “R” were applied for analysis. The
clustering of the samples indicates that each species contains less within-population genetic
variability. Same pattern was observed when sequence of three genes was combined and MDS
plot was constructed. Phylogenetic analysis of the gene sequences revealed that each species
comprised a clade that is clearly distinct from the clade comprised of other species of deer
selected for this study. Finding of this study indicated that these species of deer have significant
genetic variations among-species that differentiate them from each other. This is the first report
from our region. The information of selected species of deer is prerequisite for designing
effective strategy in future conservation practices. However further genomic investigations
should be carried out at larger scale. Availability: Items available for loan: UVAS Library [Call number: 2560-T] (1).
161.
Identification Of Genetic Variants In The Low Density Lipoprotein Receptor Gene Causing Familial Hypercholesterolemia And Its Sequence Homology With Mus Musculus
by Ameer Hassan (2014-VA-504) | Dr. Wasim Shehzad | Dr. Muhammad Yasir Zahoor | Dr. Muhammad Tayyab.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Familial hypercholesterolemia (FH) is caused by mutations in the genes coding for the low-density lipoprotein receptor (LDLR), apolipoprotein B-100, or proprotein convertase subtilisin/kexin type 9 (PCSK9). In this study, a molecular analysis of LDLR gene was performed in 20 unrelated patients from Pakistan. All patients were clinically diagnosed with definite or possible hypercholesterolemia according to a uniform protocol and internationally accepted WHO criteria. Preferable study was made to highlight the Genetic variation in Exon 4 of LDLR gene associated with defective catabolism of cholesterol effecting lipid metabolism which results in Familial Hypercholesterolemia. The extraction of genomic DNA was done from all selected blood samples. By selecting primers they were synthesized and optimized on extracted DNA samples. PCR product was sequenced and aligned. Mutations in the LDLR gene and its sequenced homology with Mus musculus were analyzed. We didn’t found any polymorphisms in the LDLR gene exon 4. So we concluded that there is no association between SNPs and increased levels of cholesterol in Pakistani population. More research should be carried out in Pakistan by increasing the sample size and considering the other regions of LDLR gene. This study will help the early detection and treatment of such cases and may ultimately reduce the incidence of mortality due to myocardial infarction. Apart from diagnosis, we also suggest it will be a potential therapeutic strategy to manage FH. Availability: Items available for loan: UVAS Library [Call number: 2538-T] (1).
162.
Molecular Investigation Of Low Density Lipoprotein Receptor Gene Causing Familial Hypercholesterolemia And Its Evolutionary Relationship With Pan Troglodytes
by Rida Zainab (2014-VA-808) | Dr. Maryam Javed | Dr. Asif Nadeem | Prof. Dr. Tahir Yaqub.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Familial Hypercholesterolemia (FH) phenotype is related to improper metabolism of low density lipoproteins due to mutations in Low-density lipoprotein receptor (LDLR) gene with increased risk of ischemic heart disease. Genetic variants in LDLR gene are associated with defective catabolism of cholesterol effecting lipid metabolism which results in familial hypercholesterolemia. It occurs in both forms: Homozygous Familial Hypercholesterolemia and Heterozygous Familial Hypercholesterolemia.
Patients having high cholesterol were identified by observing the values of their serum lipid profile test reports. Their detailed history was taken and blood samples from the identified patients of familial hypercholesterolemia were collected. DNA extraction was done by Organic method. Primers were synthesized and PCR was conducted using optimized recipe and conditions. PCR products were sequenced.
Sequenced data was analyzed using Chromas or BioEdit software. BLAST was performed and sequences were aligned individually by comparing it to the reference sequence. This showed difference in any specific position of a mutated sequence against the reference sequence. CLUSTALW aligned all the sequences together in one time. Sequences were compared with reference sequence to detect the presence of any mutation or SNPs.
SNPs were identified manually and the peaks were observed in order to determine if the genotype is heterozygous or homozygous. Statistical Analysis was done and any amino acid change due to the observed SNPs was determined by using Expasy Translate Tool. It was found that both the SNPs showed amino acid changes. In the end, homology analysis was done which showed that Homo sapiens had their LDLR gene closest to that of Gorilla gorilla gorilla. Availability: Items available for loan: UVAS Library [Call number: 2551-T] (1).
163.
Trypanosomiasis and Leishmaniasis
by Hide, G.
Edition: 1stMaterial type: ; Literary form:
not fiction
Publisher: Oxon: CABI; 1997Availability: Items available for loan: Pattoki Library [Call number: 616.9363 Hide 15543 1st 1997 Zoology] (1).
164.
Polymorphism Analysis Within Tata-Box Of Bovine Lactoferrin Gene And Its Association With Mastitis In Sahiwal Cows
by Kashmala Haroon (2014-VA-04) | Dr. Sehrish Firyal | Dr. Immad Rashid | Dr. Muhammad Tayyab.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Mastitis is one of the most important diseases in dairy cows throughout the world, and is responsible for significant economic losses to the dairy industry especially in Pakistan. Several factors are responsible for this disease and about 20% bovines are suffering with this disease. Mastitis susceptibility and resistance is influenced by genetic variation of animals. Variations to polymorphisms in LF gene assume critical part of the immune response to mastitis. Polymorphism within LF gene may influence immune response to the mastitis in bovines. Recent study shows that promoter region of LF gene is highly polymorphic among bovines.
Present study was planned to identify polymorphism analysis within TATA-box of bovine LF gene and its association with mastitis. Multiple blood samples were collected from Sahiwal cows having clinical and sub-clinical mastitis. 10 samples were collected as a control. DNA extraction was done by organic extraction method and then quantification was done by Nanodrop. Amplification and sequencing was performed to get desire sequence of the gene. Comparative study of obtained sequence results were analyzed by using NCBI blast. Bioinformatics analysis was done with the help CLUSTAL W and BioEdit softwares.
Two novels and one reported SNPs were discovered within TATA-box of LF gene that might be having strong genetic association with mastitis in Sahiwal cows. This gene is strong candidate gene to differentiate between mastitis susceptible and resistant Sahiwal cows.
Availability: Items available for loan: UVAS Library [Call number: 2584-T] (1).
165.
Polymorphism Analysis Of Exon 2,5 And 10 Of Bovine Lactoferrin Gene And Its Association Within Mastitis In Sahiwal Cows
by Sidra Mukhtar (2014-VA-223) | Dr. Sehrish Firyal | Dr. Sultan Ali | Dr. Muhammad Wasim | Dr. Muhammad Avais.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: A few factors militate against understanding the milk production capability of bovines. Mastitis is the most costly and the prevalent production-limiting disease of dairy animals in Pakistan and somewhere else on the world. Susceptibility and resistance to mastitis is a complex characteristic and impacted by hereditary variation of animals. Among these variations, the polymorphisms in LF assumes critical part in the immune responses to mastitis.
Susceptibility and resistant to mastitis is a complex trait and influenced by genetic variation of the immunity genes of the animals. Among these variations, polymorphism in Lactoferrin gene (LF) play important role in immune responses to mastitis. Polymorphism in exons 2, 5 and 10 of Lactoferrin gene are associated with mastitis susceptibility and resistance. It is a potential candidate gene for screening of the mastitis susceptible and resistant dairy cows.
The present study was designed for the identification of polymorphism in LF gene associated with mastitis. Blood samples from 20 Sahiwal cows having clinical and subclinical mastitis were sampled. Blood samples of 10 normal Sahiwal cows were also collected. DNA was extracted. Specific primers for amplification of LF gene were designed by using Primer 3 software.
LF gene was amplified and sequenced to get the full length sequence of the gene. Comparative analysis of resulted sequences was done with the help of NCBI BLAST. Multiple sequence alignment was done by using CLUSTAL W and BioEdit softwares. Protein analysis was done with ExPasy translate tool and the development of 3D structure were using PYMOL software.
Availability: Items available for loan: UVAS Library [Call number: 2582-T] (1).
166.
Sequence Analysis Of Mitochondrial Atpase 8/6 Gene Variants In Sindh Ibex (Capra Aegagrus Blythi)
by Javeria Zafar (2014-VA-222) | Dr. Asif Nadeem | Dr. Maryam Javed | Prof. Dr. Abu Saeed Hashmi.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: ATPase 8/6 gene plays a vital role in survival of an organism by generating energy in the form of ATP synthase. Considering the importance of ATPase8/6 gene in energy generating, present study has been designed to characterize this gene in Sindh ibex. The characterization of ATPase8/6 gene might be helpful for deriving phylogenetic relationship among different species and identifying new functions among the related species. Tissue/blood samples (n=15) were collected from Kirthar National Park, Sindh. Standard DNA extraction method was used for DNA extraction. PCR primers were designed by Primer3 software and amplification of gene was done by Polymerase Chain Reaction. PCR product was sequenced bi-directionally by Big Dye TM Terminator on ABI 3130XL Genetic Analyzer. Multiple sequence alignment was performed for polymorphism identification. Genetic diversity was calculated by using DNAsp. Phylogenetic analysis using the MEGA6 software package and an equally weighted maximum parsimony analysis was performed using the close-neighbor-interchange algorithm. The results indicated that Sindh ibex ATPase8/6 gene was highly similar to Capra caucasica. The results of this data might be helpful in designing effective conservation strategies of different species of wild animal. Availability: Items available for loan: UVAS Library [Call number: 2587-T] (1).
167.
Principles of Cell Biology
by Plopper, George.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: USA: Jones & Bartlett; 2016Availability: Items available for loan: UVAS Library [Call number: 571.6 Plopper 31735 2nd 2016 Genetics] (1).
168.
Biology / 12th ed.
by Mader, Sylvia S.
Edition: 12th ed. Material type: ; Literary form:
not fiction
Publisher: USA: McGraw Hill; 2016Availability: Items available for loan: Pattoki Library [Call number: 570 Mader 31727 12th 2016 Zoology] (1).
169.
Development Of Novel Mtdna Metabarcodes For The Species Differentiation Of Class Aves
by Syeda Rida Mehak Sherazi (2010-VA-477) | Dr. Muhammad Imran | Dr. M. Yasir Zahoor | Mr. Shahid Abbas.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: The Folmer COI mtDNA universal primers that are considered standard for DNA barcoding of life contain so many mismatches against the target sequences of vertebrate origin that they often end in failure to amplify many of vertebrate DNA extractions. This discrepancy favors for the selection and designing of new metabarcode primers that can be used to identify all individuals of vertebrates or at least all individuals represented in a class of Vertebrata such as Class Aves. The current study embarks on such an endeavor. In this study development of new mtDNA metabarcode (16SrRNA) that can be used as universal primers to amplify almost all species of Class Aves for different forensic and molecular biodiversity analyses.
Blood/feather/tissue samples were collected from Class Aves (one specimen from every order reported to be present in Pakistan). DNA was extracted from the collected specimens through standard organic method, qualified and quantified and then PCR-amplified using novel universal primers selected from aligned mtDNA sequences originating from all Aves mitochondrial DNA genomes submitted to different online sequence databases such as NCBI nucleotide database. The sensitivity of PCR also be assessed using a range of DNA concentrations. The amplified products were sequenced on ABI Genetic Analyzer following Sanger’s dideoxy method of sequencing. The correctness of obtained mtDNA sequences were examined visually in Chromas Lite 2.1 software and then alignment of these sequences were performed against highly similar DNA sequences in NCBI nucleotide databases using BLAST in order to identify origin of unknown mtDNA sequences. With the help of sequencing and phylogenetic studies specificity of the universal primer set confirmed and presented as a novel metabarcode (16SrRNA) for species level identification of large number of Avian species
In summary, we present universal method for species classification of Aves using a targeted parallel sequencing approach. Both sequencing and phylogenetic studies experiments confirm
Summary
82
specificity of universal primer set. Although promising results were obtained with current settings, rapid improvement of bench top instruments will further develop method with less hands-on, fewer sequencing errors and lower detection limit. So, in future, this barcode can be used for species identification in various fields of study such as meat adulteration, illegal trade, food mislabeling and molecular estimation of biodiversity. Availability: Items available for loan: UVAS Library [Call number: 2617-T] (1).
170.
Identification And Expression Analysis Of Genes Involved In Obsessive Compulsive Disorder In Pakistani Population
by Javeria (2008-VA-627) | Prof. Dr. Masroor Ellahi Babar | Dr. Muhammad Wasim | Prof. Dr. Muhammad Abdullah.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: The background of this study is that WHO reports that psychiatry disorders affect worldwide 0.8 to 2% population. Anxiety illnesses are a class of illness associated with unreasonable and disturbing sensation of fear and tension. There are several types of anxiety disorders, such as panic disorder, agoraphobia, specific phobia, social phobia, OCD. Obsessive-compulsive disorder is a chronic disabling condition. OCD is characterized by repetitive, intrusive thoughts, images, and impulses and by repetitive, ritualistic physical or mental acts performed to reduce the attendant anxiety. The severity of OCD depends on the amount obsessions and compulsions. The Yale-Brown Obsessive Compulsive Scale (Y-BOCS) is a reliable and consistent scoring system that can be used to categorize OCD. The major genes involve in OCD are SLC6A4, BDNF, SLC1A1 and COMT genes.
The study was enrolled patients treated for OCD. Blood samples have been collected from the patients. DNA extracted from fresh blood. Primers were designed. Then DNA amplification have done by Bio-Rad thermal cycler. Then gel electrophoresis was done for PCR product quantification. PCR products precipitated and sequenced. SNPs were identified. Real-time quantitative RT-PCR was performed for each sample with TaqMan Universal PCR mastermix which showed down regulation of COMT gene in OCD patients in Pakistani population.
The aim of this study was SNP identification in Pakistani Population in Obsessive Compulsive disorder and to analyze the gene expression of COMT gene involved in OCD in Pakistani Population.
Availability: Items available for loan: UVAS Library [Call number: 2620-T] (1).
171.
Development Of Novel MtDNA Metabarcodes For Species Differentiation Of Class Reptilia
by Imran Tariq (2014-VA-505) | Dr. Muhammad Imran | Dr. Wasim Shehzad | Dr. Saadat Ali.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: The FoImer COI: mtDNA. universal primers that are considered standard for DNA barcoding of life contain so many =matches against the target sequences of vertebrate men tat they often end in failure to amplify many of vertebrate DNA eurections. This disaepancy fawn foe the seaman and designing of new metabarcode panes that can be used m ideally all inditdrals of vertebrees or at least all individuals represented in a class of Vertebrate such as Cass Reprilia. The current study was embadang on such an endeavor
The proposed study was develop new m5DNA membarc ode that may be used as universal Kilns; to amplify almost all species of Class Repalia for different formic and molectdr biodivesity analyses.
Blood and tissue samples were collected from Class Repdha (at :east 24 species from every ceder reported to be present in Pakistan) DNA was extracted from the collected specimen through stacdasd organic method. qualified and =meted and then PCR-amplified using novel universal primers selected from aligned =DNA sequences origtadng from all repdlian mitochondria DNA pnomes submitted to diens online sequence databases such as NCB: micleotide database. Tne sensitiviry. of PCR was assessed using a range of DNA come:madam. The amplified products were sequenced on A131 Genetic Analyzed following Sarge's dideacy method of sequencing.
The correctness of obtained croDNA sequences were examined visually in Chromes Lite 2.1 software and then alipmmt of these sequences were per: waxed agitinc highly similar DNA sequences in NCBI nu6eonde databases using BLAST in order to identify the coigin of la-noun =DNA sequences
sequencing everimeas and phyla...net< studies was confirm the specificity of the universal primer set developed and present a novel metabarcode for species level identification of large number of reptelian species. So, In future this barcode can be used for species identification in various fields of study such as illegal trade and molecular estimation of boidiversity.
Availability: Items available for loan: UVAS Library [Call number: 2628-T] (1).
172.
Homology & Polymorphism Analysis Of Cc2d1a Gene In Human And Canine For Cognitive Function
by Hafiz Qamar Abbas (2014-VA-214) | Dr. Muhammad Yasir Zahoor | Dr. Wasim Shehzad | Dr. Saadat Ali.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Cognitive disability is a group of genetically heterogeneous abnormality that leads to variable degrees of cognition deficits. It has been shown that inherited disorders can be caused by mutations in large number of different genes and there is evidence for the presence of as yet unknown genes in a significant proportion of patients. This disease can affect 1-3% of overall population and higher in consanguineous families. We aimed to identifying the homology and polymorphism of the gene CC2D1A between human and canines. The present research work was carried out in four phases. The first phase was including enrolment of 10 affected non relevant families with disease history and consent was taken on consent forms as approved by IRB, UVAS. Secondly DNA extraction was done by using standard lab protocols. Thirdly amplification of the selected domains of selected gene (CC2D1A) was done through PCR amplification after designing primers of the selected domains. Sequencing of the amplified products has to be done through Sanger method and mutation analysis was conducted for variants We found two new asynonymous mutation one is deletion of c. 1664_1664delA which lead to the change in the normal function of protein (88%) and other is heterozygous mutation c.1921A/T that result in amino acid change from R to W (12%). Whereas homology analysis shows that deletion region is partially conserved as it code different amino acid but some key domains are conserved. This homology shows that deletion in this region can change the protein expression which can relate to unconscious condition like behavioral or mental retardation. This will be helpful in providing genetic counseling services to indigenous population for intellectual disability cases. Availability: Items available for loan: UVAS Library [Call number: 2627-T] (1).
173.
Introduction to Biotechnology / 3rd ed.
by Thieman, William J | Palladino, Michael A.
Material type: ; Literary form:
not fiction
Publisher: India: Pearson Education, 2014Availability: Items available for loan: UVAS Library [Call number: 660.6 Thieman 31793 2nd 2014 Biotechnology] (4). Checked out (1).
174.
Biochemistry, Molecular Biology And Genetics / 6th ed.
by Lieberman, Michael A.
Edition: 6th ed.Material type: ; Literary form:
not fiction
Publisher: New Delhi: Wolters & Kluwers; 2014Availability: Items available for loan: UVAS Library [Call number: 572.8076 Lieberman 31789 6th 2014 Biochemistry] (1).
175.
Calculations in Molecular Biology and Biotechnology : A Guide to Mathematics in the Laboratory / 2nd ed.
by Stephenson, Frank H.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: India: Academic Press; 2014Availability: Items available for loan: UVAS Library [Call number: 572.80151 Stephenson 31847 2nd 2014 Biotechnology] (1).
176.
Molecular Characterization Of Canine Babesiosis In Ticks And Dogs
by Tahira Sarwar (2014-VA-523) | Dr. Wasim Shehzad | Dr. M. Yasir Zahoor | Dr. Ali Ahmed Sheikh.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Babesia canis is an intra-erythrocytic parasite which cause canine babesiosis in both animals and humans. Currently, there are three sub-species of Babesia canis has been identified i.e Babesia canis canis , Babesia canis vogeli and Babesia canis rossi. Currently used diagnostic methods are clinical symptoms, peripheral blood smear microscopy and serological tests (IFAT and ELISA).Current study was conducted to compare the specificity and sensitivity of blood smear microscopy and PCR techniques to diagnose canine babesiosis. This study is comparative as well as developmental in nature. Although peripheral blood smear microscopy is cost effective and quick method of diagnosis in case of high or moderate parasitaemia in blood. But the limitations associated with microscopy include false negative diagnosis in case of low parasitaemia in chronic and asymptomatic infection,morphological similarity of Babesia with other species of Plasmodium and Theileria these limitations may lead to misdiagnose the infection due to which disease may remain unnoticed.Total 50 samples comprising of 25 blood samples and 25 ticks were collected randomly from infected dogs from June, 2015 to November, 2015. These samples were screened microscopically as well as with PCR. Out of 50 samples of dogs and ticks, 18 samples found to be positive for the Babesia canis. 11 samples are Babesia canis vogeli and 07 samples are Babesia canis canis were to be identified in positive samples of dogs and ticks.The results obtained from the study clearly show that PCR is more reliable, precise and sensitive assay for diagnosis of canine babesiosis then microscopy. It is hoped that proposed method to diagnose babesiosis will help to nullify the problems associated with microscopy. This will ultimately facilitate in the formulation of effective treatment control and vaccine development strategies which may eradicate babesiosis. Availability: Items available for loan: UVAS Library [Call number: 2642-T] (1).
177.
Campbell Biology / 9th ed.
by Reece, Jane, B.
Edition: 9th ed. Material type: ; Literary form:
not fiction
Publisher: USA: Pearson; 2005Availability: Items available for loan: Pattoki Library [Call number: 570 Reece 29599 9th 2005 Biology/Wildlife] (1).
178.
Bioinformatics: Principals and Applications
by Bal, Harshwardhan P | Harshawardhan P. Bal.
Edition: 1stMaterial type: ; Literary form:
not fiction
Publisher: New Delhi: Tata McGraw Hill 2005Availability: Items available for loan: UVAS Library [Call number: 570.285 Bal 24512 1st 2005 Managment] (1).
179.
Genetic Identification And Characterization Of Pakistani Birds Of Perdicinae Subfamily (Partridge) Through Dna Barcoding Method
by Asim Iqbal Jutt (2013-VA-557) | Dr. Ali Raza Awan | Dr. Muhammad Wasim | Prof. Dr. Kamran Ashraf.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Pakistani birds of Perdicinae sub family are cage and game birds. Birds includes Altectoris chukar, Ammoperdix heyi, Ammoperdix griseogularis, Francolinus francolinus and Francolins pondicerianus. Traditional methods of identification were based on the phenotypical characterization of birds, which may lead to incorrect identification, so there was need to explore their characters at DNA level for accurate identification and to establish a DNA reference.
Birds of sub-family Perdicinae have not been genetically characterized in Pakistan. A new precise method “DNA barcoding” was applied using COI gene of mDNA for authentic identification and classification of these birds. Blood and tissue samples of five species (fifteen samples) were obtained. DNA of each sample was extracted by organic method. Amplification of CO1 gene was done by using a universal set of primers BIRDF1, BIRDR1. Sequence of 450bp were analyzed using bioinformatics softwares. Each sample was aligned with its reference sequence of COI gene available on NCBI. Every nucleotide position which did not align with the reference sequence was studied to identify SNPs. A common phylogenetic tree of all partridges showed that they have common ancestor about 0.7 million year ago, F.francolinus, F.pondicerianus and A.heyi shared a common clade whereas A.chukar made a separate clade from the ancestor. A.heyi and F.pondicerianus showed closed resemblance. It has been proved that DNA barcoding is an efficient and accurate molecular tool for species identification and phylogenetic implication. This study established a DNA Data Bank that helped scientists to investigate the biodiversity, taxonomic classification, species identification and also established foundations for molecular biologists to study taxonomic uncertainties at sub species level using SNP based identifying marker. Availability: Items available for loan: UVAS Library [Call number: 2714-T] (1).
180.
Molecular Biotechnology
by Dehlinger, Carolyn.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: USA: Jones and Bartlett; 2016Availability: Items available for loan: UVAS Library [Call number: 572.838 Dehlinger 32226 1st 2016 Biotechnology] (1).
181.
Organic Evolution / 2nd ed.
by Kavita, Dr | Arora, Dr. M.P.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: India: A.I.T.B.S Publishers; 2017Availability: Items available for loan: Pattoki Library [Call number: 575 Kavita 32215 2nd 2017 Zoology] (1).
182.
Dictionary of Developmental Biology and Embryology / 2nd ed.
by Dye, Frank J.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: USA: Wiley Blackwell; 2012Availability: Items available for loan: UVAS Library [Call number: 571.803 Dye 32287 2nd 2012 Theriogenology] (1).
183.
Statistical Methods in Molecular Evolution
by Nielsen, Rasmus.
Edition: 1st ed. Material type: ; Literary form:
not fiction
Publisher: New Delhi: Springer; 2005Availability: Items available for loan: UVAS Library [Call number: 576.8 Nielsen 32281 1st 2005 Genetics] (1).
184.
Cell and Molecular Biology : Concepts and Experiments / 6th ed
by Karp, Gerald.
Edition: 6th ed.Material type: ; Format:
print
; Literary form:
not fiction
Publisher: New York : John Wiley & Sons, 2010Availability: Items available for loan: UVAS Library [Call number: 575.1 Karp 32259 6th 2010 Genetics] (1).
185.
Molecular Data Analysis Using R
by Ortutay, Csaba | Ortutay, Zsuzsanna.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: Singapore: Wiley Blackwell; 2017Availability: Items available for loan: UVAS Library [Call number: 572.33 Ortutay 32257 1st 2017 Statistics] (1).
186.
Handbook on Ingredients for Aquaculture Feeds
by Hertrampf, Joachim W.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: India: Springer; 2000Availability: Items available for loan: Pattoki Library [Call number: 577.6 Hertrampf 32260 1st 2000 Fisheries] (1).
187.
Developmental Biology / 11th ed.
by Gilbert, Scott F.
Edition: 11th ed.Material type: ; Literary form:
not fiction
Publisher: USA: Sinauer Associates Inc; 2016Availability: Items available for loan: UVAS Library [Call number: 571.8 Gilbert 32252 11th 2016 Theriogenology] (1).
188.
Molecular Biology / 4th ed.
by Weaver, Robert Franklin.
Edition: 4th ed. Material type: ; Literary form:
not fiction
Publisher: USA: McGraw Hill; 2008Availability: Items available for loan: UVAS Library [Call number: 572.8 Weaver 32245 4th 2008 Genetics] (1).
189.
Plant Cell Biology: From Astronomy to Zoology
by Wayne, Randy.
Edition: 1st ed. Material type: ; Literary form:
not fiction
Publisher: USA: AP; 2009Availability: Items available for loan: Pattoki Library [Call number: 571.62 Wayne 32246 1st 2009 Wildlife] (1).
190.
Animal Diversity
by Hickman, Cleveland P., Jr | Larson, Allan | Roberts, Larry S.
Edition: 1st ed. Material type: ; Literary form:
not fiction
Publisher: USA: McGraw Hill; 2003Availability: Items available for loan: Pattoki Library [Call number: 590 Hickman 32244 1st 2003 Wildlife] (1).
191.
The science of life/ 1st ed.
by Lawrence S. Diloon.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: USA: The Macmilan Company; 1964Availability: Items available for loan: UVAS Library [Call number: 570 Lawrence 11001 1st 1964 Biology] (1).
192.
World animal science
by Morely | Morley.
Edition: 1st Material type: ; Literary form:
not fiction
Publisher: New York; Elsever; 1981Availability: Items available for loan: UVAS Library [Call number: 636.085 Morley, F H W 11602 1st 1981 Biology] (1).
193.
The dictionary of the biological sciences / 1st ed.
by Gray Peter.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: USA: Reinhold publishing corporation; 1967Availability: Items available for loan: Pattoki Library [Call number: 574.03 Peter 12173 1st 1967 Genetics] (1).
194.
Introduction to molecular immunology/ 1st ed.
by Alfred Ninsonoff.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: USA: 1982Availability: Items available for loan: UVAS Library [Call number: 616.079 Alfred 12373 1st 1982 Molecular.Biology] (1).
195.
The Molcelar Biology Of Vireses
Edition: 1stMaterial type: ; Literary form:
not fiction
Publisher: Cambridge; general Microbiology; 1968Availability: Items available for loan: UVAS Library [Call number: 576.64 10654 1st Biology] (1).
196.
Vertebrate adaptations; readings from Scientific American/ 15th ed.
by Norman K | Wessells.
Edition: 15th ed.Material type: ; Literary form:
not fiction
Publisher: USA: 1968Availability: Items available for loan: Pattoki Library [Call number: 596.05 Norman 12206 15th 1968 Fisheries] (1).
197.
Introduction To Virology
by Smith, Kenneth M.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: London: Chapman and Hall; 1980Availability: Items available for loan: UVAS Library [Call number: 576.64 Smith 11480 1st 1980 Biology] (1).
198.
Comparative Immunology
by Cooper | Edwin L.
Edition: 1st Material type: ; Literary form:
not fiction
Publisher: London: Prentice Hall International; 1976Availability: Items available for loan: UVAS Library [Call number: 591.29 Cooper 10745 1st 1976 Micro.biology] (1).
199.
Statistical methods in biology
by Norman | Norman | Bailey, Norman T. J.
Edition: 2ndMaterial type: ; Literary form:
not fiction
Publisher: London; Hodder And Stoughton; 1981Availability: No items available
200.
The problem of chemical and biological warfare : a study of the historical, technical, military, legal and political aspects of CBW and possible disarmament measures
by Stockholm | Stockholm | Stockholm International Peace Research Institute.
Edition: 1stMaterial type: ; Literary form:
not fiction
Publisher: London; Stockholm International Peace Institute; 1973Availability: Items available for loan: UVAS Library [Call number: 358.38 Stockholm 11105 V.31st 1973 Biology] (1).
