1.
Post Vaccination Antibody Profile Of Haemorrhagic Septicaemia In Adult Buffaloes
by Najam Raza Naqvi, S | Ata-Ur-Rizvi | Mubasher | Muhammed Amin Sheikh | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1989Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0151,T] (1).
2.
Comparative Efficacy Of Rose Bengal Plate Test (Rbpt) And Srum Agglutination Text (Sat)
by Shabbir Ahmad | Muhammed Ajmal | Mubasher | Muhammed Amin Sheikh | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1984Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0152,T] (1).
3.
The Role Of Escherichia Coli As Poultry Pathogen
by Rauf Ahmad | Sheikh Muhammed Amin | Asif Rabbani | Muhammed Ajmal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1986Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0153,T] (1).
4.
Incidence Of Rabies Virus In Saliva Of Apparently Normal Dogs In Lahore
by Ajmal Hussain, S | Muhammed Naeem | Asif Rabbani | Ata-Ur-Rehman Rizvi | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1986Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0154,T] (1).
5.
A Comparative Study On The Efficacy Of Two Newcastle Disease Vaccines Administered Through Aerosol Method,
by Saeed, M | Sheikh Muhammed Amin | Muhammed Ajmal | Sheikh Altaf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1984Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0155,T] (1).
6.
Preliminery Studies On Hydropericardium Syndrome In Broiler
by Khurshid Ahmad | Muhammed Ajmal | Muhammed Akram Muneer | Zafar Iqbal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1989Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0156,T] (1).
7.
Poultry Meat As A Source Of Human Salmonellosis
by Saba Mahmood | Muhammed Ajmal | Syed Ata-Ur-Rehman Rizvi | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1987Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0157,T] (1).
8.
Preparation Of Fowl Pox Vaccine On Tissue Culture
by Qamar-ul-Islam | Muhammed Yousaf Vaid | Muhammed Akram Muneer | Sheikh Altaf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1980Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0158,T] (1).
9.
Study Of The Immunomodulatory Effects Of Aflatoxins On The Development Of Immunity Against Newcastle Disease
by Khawar Mahboob | Ata-Ur -Rehman Rizvi | Asif Rabbani | Muhammed Ajmal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1987Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0159,T] (1).
10.
Comparison Of Indirect Haemagglutination (Ish) And Mouse Protection Test For Measuring Immunity
by Afzal, M | Sheikh Muhammed Amin | Muhammed | Muhammed Ajmal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0161,T] (1).
11.
Post Vaccination Antibody Profile Of Haemorrhagic Septicaemia In Adult Cattle
by Anjum Nisar | Muhammed Ajmal | Mubasher | S. Ata- Ur - Rehman Rizvi | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1988Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0163,T] (1).
12.
Studies On The Isolation And Serotyping Of Salmonellae From Faecal Material Of Sheep And Goats From Different Markets
by Imtiaz Ahmad, Bhuttar | Ata - Ur - Rehman Rizvi | Mubasher | Muhammed Narrm | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1986Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0166,T] (1).
13.
Sero-Epidemiological Survey Of Bovine Viral Diarrhea In Lahore Using Agar Gel Diffusion Technique, With An Attempt On Isolation of Virus
by Fahim Ahmad, Bhatti | Ata Ur -Rehman Rizvi | Muhammed | Muhammed Amin Sheikh | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1989Dissertation note: The seroloical study was carried out on one thousand serum samples collected randomly from abbatoirs at Lahore city. Of these serum samples 645 were from buffaloes while 55 were of cattle origin. This study was undertaken to study- the seroepidemiology off bovine viral diarrhea in and around Lahore city. Agar gel precipitation test was used for detecting antibodies in the Sera of animals. The primary bovine kidney cell cultures were used for culturing the bovine viral diarrhea virus and the antigen for immuno-diffusion tests was prepared from the infected cells.
An overall incidence of 8.6% was revealed by the agar-gel immuno-diffusion tests. The tests showed 914% buffaloes as having antibodies against bovine viral diarrhea, while '76% of the cattle showed positive results.
The influence of various epidemiologi- cal factors was proven. Higher prevalence rate was observed in the buffaloes as compared to the cattle. The age group of the animals was also found to be one of the factors affecting the distribution of bovine viral diarrhea antibodies, as maximum number of reactors were detected in the 10 years and above age group through agar-gel precipitation tests. Attempts were made on the isolation of virus on primary bovine kidney cell cultures, but were not successful.
The results of the study revealed few animals having precipitating antibodies in their sera. It shows that the disease is not widespread amongst the animals in our area; bit 8till calls for an emergent response from
experts and relevant agencies for mass scale surveys to get a better understanding of the disease and its epidemiology.
Bovine viral diarrhea - Mucosal disease complex has a great economic impact on the livestock industry in general and on the dairy and breeding programmes in particular. As Pakistan is a developing country and the economy relies heavily on the agriculture and livestock resources, therefore, control measures should be adopted without delay to have a proper check on this malady before it spreads to an un-controlable extent.
Availability: Items available for loan: UVAS Library [Call number: 0167,T] (1).
14.
A Comparative Study Of Brucellosis In Livestock And Human Beings
by Amra Akram | Muhammed Ajmal | Ata- Ur -Rehman Rizvi | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1991Dissertation note: Seroprevalence of brucellosis in 541 cattle, 708 sheep, 780 goats and 63 human beings of one farm and 189 cattle, 125 buffaloes, 68 goats and 51 human beings of the other farm was studied. The various serologic tests used for this investigation included the slide agglutination test for initial screening, and the standard tube agglutination test (SAT) and the enzyme-linked immunosorbent assay (ELIS1) for further processing of the sera i.e. quantitation of Brucella antibodies. The higher prevalence of the disease was observed in cattle than buffaloes while goats outnumbered sheep in this respect. The prevalence of the disease in human beings was found to be related positively with the prevalence of the disease in animals.
The overall prevalence of the disease in sheep of one farm was found to be 35(4.947.), 27(3.817.) and 29(4.097.), respectively, by the slide agglutination, standard tube agglutination test (SPIT) and ELISA. Goats of one farm displayed a prevalence of 202(25.897.), 173(22.187.) and 183(23.467.) and that of the other, 3(4.417.), 2(2.947.) and 2(2.947.), respectively, by the slide agglutination, SPT and ELISA. This remarkable difference in the incidence of the disease in two farms may be attributed to the difference of
sample size.
A prevalence of 127(23.48%), 87 (16.08%) and 91(16.82%) was recorded in cattle of one farm while 30(15.877.), 19(10.057.) and 20(10.587.) cattle of the other proved positive respectively to the slide agglutination, SpiT and ELIS. In buffaloes, a prevalence of 17(13.67.) and 11(8.87.) and 11(8.97.) was noted by the slide agglutination, ST and ELISA tests, respectively. While interpreting the age-group relationship of the disease, it was found that adult and old animals had a higher prevalence than the young animals. Owing to the small number of male serum samples, the sex- based analysis of the disease could not have been adequately discussed
The enzyme linked immunosorbent assay was found to be a sensitive approach in detecting anti-Brucella antibodies than the slide agglutination and standard tube agglutination tests. The ELISA titres were, on average, about 8 times higher than the corresponding SAT titres
The results of this study have revealed an alarming prevalence of brucellosis in animals of farms which calls for an emergent response of experts for reappraisal and reassessment of the present brucellosis control situation, especially when the disease is an important zoonosis and a potential threat to the human health.
Availability: Items available for loan: UVAS Library [Call number: 0168,T] (1).
15.
Isolation And Identification Of Clostridium Tetani From Oil And Dust Samples, From Various Sources In Lahore, and Study of Their Pathogenicity
by Neelam Muizzuddin | Mukhtar Ahmed Chaudry | Muhammed Naeem | Tufail | Faculty of Veterinary Sciences.
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Publisher: 1982Dissertation note: Tetanus is a sporadic disease caused by infection with Clostridiurn tetani, a rod shaped anaerobic organism which has been frequently isolated from soil and the faeces of man and animals especially horses.
Two hundred soil and dust samples from different areas of Lahore were studies for the occurance of Clostridiurn tetani. Out of these 28 (13%) were positive for the presence of Clostridium tetani. Of these 26 positive samples, 4 out of 50 (8%) were from roads and areas not frequently used by horses: 9 out of 50 (18%) from roads frequently used by horses; 6 out of 50 (12%) from horse stables and 14% positive from the Veterinary Hospital. The results were significant.
The samples were cultured on five different media namely cooked meat medium, nutrient broth, thioglycollate broth,nutrient agar and blood agar. A comparison of the growth media showed a highly significant result.
Study of pathogenicity indicated a total number of 17 pathogenic samples out of the 26 samples positive, for Clostridium tetani. LD titre indicated the highest frequency (35.3%) between range of io425 and 10475number of organisms per ml. of medium.
Availability: Items available for loan: UVAS Library [Call number: 0169,T] (1).
16.
Studies On The Isolation And Serotyping Of Salmonellae From Mesenteric Lymph Nodes And Faecal Samples Of Camels From Lahore Abattoir Lahore Abattoir
by Javaid Masood | Muhammed Naeem | Ata - Ur - Rehman Rizvi | Manzoor | Faculty of Veterinary Sciences.
Material type: ; Format:
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not fiction
Publisher: 1986Dissertation note: The study was undertaken to detrmine the Salmonellae carrier state in camel, in an attempt to understand the epidemiologica] role played by this animal in transmission of Salmonellosis to other animals and man
The faecal and mesenteric lymph gland samples were collected from 100 apparently healthy camel a slaughtered at Lahore abattoir. Each sample was enriched in selenite troth arid isolates passed through a series of differential and selective media for an effective primary isolation and purification of Saimonellae.
The identification of isolates was carried out on the basis of their morphology, biochemical characteristics and serology. The serology was carried out using standard polyvalent "0", and "H" group arid type specific antisera by rapid spot agglutination test.
The Salinonellae were isolated from isolated from any faecal samples, giving an isolation percentage of 2.0 in this species.
On the basis of biochemical studies and serology it was observed that both the isolated strains of Salmonella belonged to Salmonella typhimrium.
The camel was found to be carrier of Salmonella typhimurium and could act as a source of infection for animals and man. Further work on large scale is recommended.
Availability: Items available for loan: UVAS Library [Call number: 0171,T] (1).
17.
Immune Response In Chicks Administered Mukteswar And Lasota Strain Newcastle Disease Vaccines Through Different Routes (Intraocular, Drinking Water, Subcutanequsly And Intramuscularly)
by Raza-ul-Haq | Muham,med Akram Muneer | Ata Ur | Rashid Ahmed Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1989Dissertation note: This study was under-taken to compare the immune response of 6 weeks old chickens which were administered Mukteswar and LaSota Strain NDV Vaccines through different routes (intraocular, drinking water, subcutaneous or intramuscular).
Birds in groups E and G which were subcutaneously and intramuscularly vaccinated with the Mukteswar strain of ND Vaccine had GMT of 181. and 315 respectively. All the vaccinates resisted virulent NDV challenge and did not show any clinical signs or untoward reaction following challenge. The groups F & H which were vaccinated subcutaneously and intramuscularly with LaSota Strain of ND Vaccine had GMT of 74 and 91, respectively. All the vaccinates of groups F and H resisted challenge. Groups A and B were administered Mukteswar and LaSota Strain of ND Vaccine intraocularly. Their GMT was 84 and 45,. respectively. LaSota Strain Vaccine conferred 80% protection while the Mukteswar Strain Vaccine afforded 92% protection.
Groups C and D were administered Mukteswar and LaSota Strain ND Vaccines through drinking water. Their GMT on 21 days postvaccination was 74 and 6.9 respectively. The postchallenge protection rate of Mukteswar and LaSota Strain vaccines was 72 percent and 68 percent, respectively.
This study indicates that the Mukteswar strain induces higher HI titre and protection percentage than the LaSota Strain irrespective of the route of administration.
Availability: Items available for loan: UVAS Library [Call number: 0173,T] (1).
18.
Study On The Immunomodulatory Effects Of Aflatoxins On The Development Of Immunity Against Fowl Cholera Vaccine In Layer Chicks
by Hamid Jalal Mian | Muhammed Ajmal | Mubasher | S. Ata - Ur -Rehman | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1988Dissertation note: The irmmunomodulatory effects of aflatoxin on the development or acquired immunity n Layers against fo-wi cholera vaccine were studled A group of 72day old Hybred layer chicks was raised on alfatoxin free feed for 6-weeks, when the experiment was started. On 42nd day of age, the birds were divided into six groups, T1, T2, T3, T4 K1, and K2
With birds in each group. A dose of 2.1 ug/g of feed was added in the ration of all the groups i.e. T1, T2, T3, except T4, which was injected i/c with single LD50) dose of aflatoxin, while K1 and K 2 were kept as vaccine no-toxin and non-toxin - non-vaccine control respectively. Group T1 was fed, toxin continuously for 42 days i.e. 42 nd of age to 84th day of age while T2 and T3 were fed toxin for 21 days, before and after vaccination i.e. 42nd to 63rd dayand 63rd to 84th day respectively. All the groups except K2 were vaccinated on 63rd day.
The antibody titres were measured, using two serological techniques i.e. indirect haemagglutination test and enzyme linked immunosorbant assay.
The results have shown that in group T1 the antibody titires of the birds could not develop to a sufficient level as compared with the vaccine control (K1) group. While in group T2 , the antibody titres rose to a sufficient level in about 14 days post-vaccination (which persisted almost on the same level till 84th day) and were quite comparable with group K but significantly higher than group T. Birds in group T3, showed a temporary increase in antibody titres which sharply decline to a very low level in about 2 weeks post-vaccination and registered a fall after the challenge. In group T4, the birds did not show any immunosuppressive reaction. The titres obtained on 70th, 77th and 84th day of age were comparable with group K1, while significantly higher from the groups T1, T3, and K2.
On the 84th day of age, all the groups were challenged with virulent strain of P.multocida Robert type-IT, which killed 11 out of twelve birds in group K2, one out of 12 in group T and 3 out of 12 in group T4, showing typical lesions of fowl cholera, while the rest 'of the birds of all groups survived one week post challenge, when the experiment was termina- ted by slaughtering the survivors. Their titres were measured and a significant decrease in titres of all the groups was recorded.
The results showed that, when aflatoxin was given continuously before and after vaccination and even if only after vaccination for a certain period of time, could greatly depress the antibody formation, but did not reduce the protective effect of vaccine on birds who despite very low titres of antibodies survived the challenge with virulent P. multocida.
Availability: Items available for loan: UVAS Library [Call number: 0175,T] (1).
19.
Studies On Pseudomonas As A Pathogen In Poultry Industry
by Iqbal Siddiqui, M | Muhammed Yousaf Vaid | Altaf Hussain Sheikh | S.M.S Jaffery | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1978Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0176,T] (1).
20.
A Study On The Incidence Of Salmonellosis In Poultry In And Around Lahore
by Zubair Anjum | Muhammed Akram muneer | Muhammed Naeem | Tufail | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1983Dissertation note: Poultry has emerged ss an imtrnrtant industry in Pakistan in recent years. The Salmonellosis in poultry has become major hinderence in the development of this industry. The imnortsnt oathoens are Salmonella gallinarum and SalmoneHa nuilorurn which mainly effct noultry.
Snot agglutintion whole-blood ranid test was conducted on 1000 birds from breeding stocks at noultrv farms and noul try cliñiôs. 17q (17.9°) reacted oositively and 100 oositive birds were selected and slaughtered and internal organs such as liver, soleen ovary and ilium were collected and their morbid material was cultured on selenite broth for 18-24 hours and then on Salmonel1a- Shigella agar for 24-48 hours at 37°C. Nutrient and MacrConkey agars were used to obtain oure colonies. Salmonella isolations were successful from 54 (54%) cases out of 100. Salmonella gallinarum from i15 (45%) cases and Salmonella pullorum from 9 (9%). The incidence due to S.gallinarum (83.33%) was higher to S.pullorum (16.67%). Different biochemical and serological tests were conducted Serologically 'D' group was confirmed.
Availability: Items available for loan: UVAS Library [Call number: 0178,T] (1).
21.
Post-Haemorrhagic Septicaemia Vaccination Serum Antibody Titre Profile In Buffalo Calves
by Shafeeq Baig, M | Muhammed Amin Sheikh | Muhammed Ajmal | Zafar Iqbal | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1989Dissertation note: A total of 60 buffalo calves, 30 from Military Farm Lahore Cantt and 30 from Livestock Experiment Station Rakh Dera Chahi Lahore were examined for post-haemorrhagic septi- cania vaccination serum antibody titre profile. The animals maintained at Livestock Experiment Station Rakh Dera Chahi were divided into group A (Age-20 months) vaccinated second time and group B (Age 3-7 months), vaccinated for the first time. The Military Farm animals were also vaccinated for the first time. The indirect haemagglutination (IRA) test and enzyme linked immunosorbent assay (ELISA) were employed for determination of antibody titres. The animals were vaccinated with alum precipitated formaline killed bacterin. The serum was collected before and after 2,7,12,16,20 and 22 weeks of vaccination from animals of Military Farm. The serum of animals from Livestock Experiment Station Rakh Dera Chahi was collected after 10,12, 16, 20, & 24 weeks of vaccination.
On the basis of indirect haemagglutination test, the immune response was very poor upto 7th week postvaccination. The maximum GMT recorded being 1.751 for animals of Military Farm. An outbreak of the disease took place at this stage killing 4 animals with the consequent revaccination of animals; increasing the GMT upto 23.508 five weeks after revaccination. The Livestock Experiment Station Rakh Dera Chahl animals showed a variable immune response in group A and B animals with a better and quick response in the former as compared to the latter. The response remained unsteady upto 12th week postvaccination and thereafter registered an increase in titres, believed to be due to an antigenic stimulus either through a natural infection or revaccination of the animals.
The absorbence values of the sera obtained through ELISA gave the same immune response profile as detected by
IHA.
Availability: Items available for loan: UVAS Library [Call number: 0179,T] (1).
22.
Diagnosis Of New Castle Disease By Fluorescent Antibody Technique
by Zahida Parveen | Ata-Ur-Rehman Rizvi | Mubasher | Muhammed Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1985Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0180,T] (1).
23.
Comparative Study On Strain Characteristics Of Streptococcus Pyogenes Isolated From Human And Animal Sources
by Anwar ul Hassan | Ata ur Rizvi | Sheikh Muhammed Amin | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1984Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0181,T] (1).
24.
Isolation Of Infectious Bursal Disease (Gumboro Disease) Virus From Infected Birds Based Upon Lesions In Embryonated Eggs
by Khalid Iqbal | Muhammed Naeem | Sheikh Muhammed Amin | Faculty of Veterinary Sciences.
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Publisher: 1988Dissertation note: A number of 30 bursae of fabricius were collected from the birds suspected for Infectious Bursal Disease, brought to the Disease Diagnostic Section, Poultry Development Centre (P.R.I.), Rawalpindi and Animal Sciences Institute, National Agriculture Research Council, Islamabad. Each sample was processed and inoculated through allantoic sac route in five embryonated eggs of 9 to 11 days. Eggs showing embryo deaths between 48 hours post inoculatiom were discarded as it was non specific embryo mortality. Five embryonated eggs were kept as control. Five serial passages were done to check the embryo mortality pattern.
Out of 30 samples, nine samples showed a typical Infectious Bursal Disease embryo mortality pattern i.e. 100% mortality at first passage, 30 to 33% mortality at second passage and 0% in subsequent passages. Seven samples showed a positive reaction in Agar Gel Precipitation Test out of nine samples. The incidence of Infectious Bursal Disease was 3 .3% in and around Rawalpindi.
Availability: Items available for loan: UVAS Library [Call number: 0183,T] (1).
25.
Studies On Duration Of Maternally Derived Antibodies Against Pasteurella Multocida In Buffalo Calves
by Fayyaz Mahmood | Muhammed Amin SHeikh | M.Sarwer Khan | Muhammed Akram Muneer | Faculty of Veterinary Sciences.
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Publisher: 1990Dissertation note: Haemorrhagic septicaemia, an imrortant bacterial disease of dairy animals (buffaloes and cattle), results due to infection of Pasteurella inultocida. Undoubtedly improved managemental practices and regular vaccination program has significantly contributed to lowering the incidence of the disease in our country, however, the pattern of the diseases has under gone changes and contrary to the past, presently the outbreaks are mostly experienced in young animals, especially, calves. The latter are invariably left unvaccinated thinking that they are safe by virtue of their passive immunity.
The present project was designed to have an idea regarding the actual period for which maternally derived antibodies were able to afford protection against any possible attack of the infection. The study was conducted on fifty pregnant, randomly selected buffaloes, maintained at livestock production and research institute Bahadarnagar Okara (LPRI).
The serum samples of the vaccinated mothers were collected a month before parturition. The serum samples of the calves were collected once before taking colostrum then at the interval of two weeks for the first four weeks. All subsequent sampling was done at an interval of four weeks. The processing of the samples for antibody titre was carried out through ELISA and haemagglutination tests.
The indirect haemgglutination and enzyme linked immunosorbent assay tests showed that the titres of maternally derived antibodies, received by taking colostrum, attained a peak during the first two weeks and gradually decreased in the following two weeks i,e up to the age of one month. At the age of 8 weeks calves were found with 0 titres.
Aanalysis of IHA revealed that the IHA titres of calves rose from 1.932 GMT to 6.824 GMT from birth to 2 weeks of age and gradually faded to 6.714 GMT after four weeks of age. calves were found with 0 titres at the age of 8 weeks.
The analysis of ELISA titres showed highest antibody titre (36.225 GMT) was in calf on 2nd week and then declined (32.633 BMT) gradually upto 4th week. At the age of 8th week calves were found with titres (4.366 GMT) almost negligible.
Buffalo dams (407.) were found with low titres when examined for their antibody level 30+10 days before parturition, despite the fact that they received vaccination according to the normal schedule of the farm.
Results of the study indicated that there was great need for improvement of vaccine and vaccine schedule i,e animals should be immunized thrice a year. Pregnant dams should be vaccinated late in gestation period so as to raise their antibody levels against the disease, ensuring a sound passive immunity of their off springs. Calves should also be regularly tested for their maternal antibodies and should be immediately vaccinated if passive protection is over. The funding of the present study necessitate the vaccination of calves at 2 months of their age.
Availability: Items available for loan: UVAS Library [Call number: 0184,T] (1).
26.
Isolation And Serotyping Of Salmonella From Wild Animals And Birds At Lahore Zoo
by Iftikhar Ahmad, Malik | Muhammed Ajmal | Ata_Ur- Rehman | Mubasher | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1989Dissertation note: The occurrence of salmonellae in a variety of zoo animals including carnivores, primates, ruminants, avifauna, rodents etc was investigated.
The faeoal samples of 500 wild animals and birds examined yielded 9 strains of salmonella, giving an incidence of 1.8%. Of these '7 strains from pigeons and rabbits were confirmed as Salmonella ty-phimuriuni and 2 strains from pheasants were confirmed as Salmonella pullorum. The relative percentage of each species was found to be 1.4% and 0.4% respectively. Salmonella typhimurium have a vex wide host range including human being. It Is therefore recommended to adopt proper hygienic measures at Lahore Zoo. Further work on a much large scale is recommended.
Availability: Items available for loan: UVAS Library [Call number: 0187,T] (1).
27.
Role Of Doves, Parrots And Quails In The Epizootiology Of Newcastle Disease
by Rauf, A | Muhammed Naeem | Manzoor | Muhammed Ajmal | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1984Dissertation note: Three species of birds i.e. doves, parrots and quails, 100 from each species were studied to investigate their role in the epizootiology of Newcastle disease. The serum of each bird was examined for the presence of antibodies to Newcastle disease virus by Haemagglutination Inhibition method using a standard Newcastle disease virus obtained from Veterinary & Research Institute, Lahore. It was observed that 46% of parrots, 29% of doves and 7% of quails had experienced Newcastle disease. The average titre for three species of birds was 76 with a range of 20-1280, being highest in parrots and lowest in quails. Attempts were made to isolate Newcastle disease virus from the faecal material and pooled organs of each bird in developing chick embryo. Five isolates of Newcastle disease virus were recovered from parrots, two from doves and none from quails. The recovered isolates possessed a mild to moderate pathogenicity for day old chicks. From these trials it was evident that doves and parrot could act as carriers of Newcastle disease and disseminate the disease to other birds over vast areas while despite their susceptibility the exact role of quails could not be determined in the epizootio- logy of Newcastle disease. Further studies on larger scale were recommended.
Availability: Items available for loan: UVAS Library [Call number: 0190,T] (1).
28.
Studies On The Isolation And Serotyping Of Salmonella From Faecal Material Of Buffaloes
by Rashid, M | Muhammed Ajmal | Mubasher | S. Ata-Ur- Rehman Rizvi | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1987Dissertation note: Health of human population alongwith animals is always at risk when Salmonella shedding carriers are present. The modern trend of Farm industry makes it more important to look out for the carriers and treat them to save the other animals and man from Salmonellosis.
The present work Was under-taken to determine the Salmonella carrier state in healthy buffaloes, in an attempt to understand the epidemiological role played by these animals in the transmission of Salmonella infection to other animals and man.
The faecal samples. were collected from 1000 animals. A series of enrichment, selective and differential media, biochemical& 6ro1ogy teste were used for isolation,iden- tification and serotyping of Sa.iionella from the faecal specimens.
It was observed that 18 animals out of 1000(1.8%)were carrier of Salmonella.
Salmonella typhimurium was isolated from 12 cases while Slamonella butantan was isolated from 6 cases. Occurrence of Salmonella typhimurium and Salmonella butantan are important from Veterinary Public Health point of view.
Availability: Items available for loan: UVAS Library [Call number: 0191,T] (1).
29.
Studies On The Isolation And Serotyping Of Salmonella From Gall-Bladder Of Buffaloes Slaughtered At Abattoirs In Lahore
by Munir Ahmad, M | S.Ata-Ur-Rehman Rizvi | Muhammed Naeem | Zafar Iqbal | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
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Publisher: 1987Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0192,T] (1).
30.
Cultural Evidence Of Organism And Specific Antibody Levels Of Past Multocida Carriers In Buffaloes
by Tahir Yaqub | Muhammed Amin Sheikh | Ata-Ur- Rehman Rizvi | Zafar Iqbal | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1988Dissertation note: Blood samples and laryngeotracheal material of 500 buffaloes, slaughtered at various abattoirs of Lahore, in the month of February, 1988 through June, 1998, were collected and processed for ascertaining carrier condition for Past. Multocida and its effect on the serum antibody levels.
Of the total number of buffaloes examined, the organism was isolated from 23 (4.6 per cent) animals when the laryngeotracheal region was swabbed directly after slauther.
The erum samples from 23 past. Multocida carrier animals were examined for the presence of specific antibodies. Consequently 19 (82.60%) animals proved positive. A similar examination was conducted on sera of 60 non-carrier animals, where, only 5 were found positive. It is hopefully thouth that the findings of this project will provide utilitarian information regarding the role of carriers in epidemiology of HS and its control.
Availability: Items available for loan: UVAS Library [Call number: 0193,T] (1).
31.
Studies On The Occurrence Of Rabies Virus In Saliva Of Apparently Normal Dogs In Lahore
by Aftab-ur-Rehman | Ata-Ur-Rehman Rizvi | Muhammed Ajmal | Faculty of Veterinary Sciences.
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Publisher: 1985Dissertation note: Rabies, is invariably a fatal viral infection of Central Nervous System effecting almost all species of warm blooded animals and man. The disease is causing considerable loss of human and animal life in the country. Sporadic cases of rabies occurred throughout the year, but during the Spring and fall the disease assumes an epidemic form. How the disease perpetuates in nature is not yet clearly understood as mortality rate is almost 100% in affected animals. Some references were available in the literature regarding the possibility of existence of survivors and carriers of' rabies in different animal species.
In order to understand the epidemiology of disease an attempt was made, in the present work, to look for the possibilities of existance of carriers among dog population in the city.
A total of 214 apparently healthy domestic dogs together with 100 stray dogs were tested for the presence of rabies antigen in the salivary gland and brain by Fluorescent antibody test. In case of stray dog rabies antigen was detected in 5 animals including 2% animals secreting virus in salivary gland and 3% animals with antigen both in salivary gland and brain, while in case of domestic do6 animals showed rabies antigen in saliva and 4 animals both in saliva and brains.
Simultaneously 48 cases suspected for rabies including 3 carcases, one cat two dogs and one human brain were also examined for rabies antigen and 50% cases were found positive.
The presence of rabies antigen in the saliva of 2 stray dogs and 2 domestic dogs was highly significant of the carrier role of dogs in rabies. Further work on much larger scale were recommended.
Availability: Items available for loan: UVAS Library [Call number: 0194,T] (1).
32.
Studies On The Isolation And Serotyuping Of Salmonella From Mesenteric Lymmphnodes Of Sheep And Goats
by Ahmad Usman Zafar | Muhammed Ajmal | Ata-Ur- Rehman Rizvi | T.M Khan | Faculty of Veterinary Sciences.
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Publisher: 1986Dissertation note: Five hundred samples of mesenteric lymph nodes of sheep and goats , 20 from each species , slaughtered at Lahore abattoir, were bacteriologically examined to determine the Salmonella carrier state in healthy sheep and goats. Nine strains of Salmonellae belonging to two serological types i.e. Salmonella typhimurium (l.40%) and Salmonella anatum (0.40%), were isolated. The incidence of Salmonellosis in sheep and goats were recorded as 2.40% and 1.20% respectively.
The media used for primary isolation and purification were Selenite broth, Bismuth sulphite agar, Brillient green agar, MacConkey agar and Nutrient agar. Results of Triple sugar iron agar and Urease test gave a preliminary confirmation of Salmonella organisms. Sugar fermentation reactions, Methyl red and Voges Proskauer tests were also employed for the confirmation of isolates. The Serological identification was carried out with the help of standard antisera manufactured by ' Research Institute of Infectious and Parasitic Diseases Sofia, Bulgaria. Further work on much larger scale is recommended.
Availability: Items available for loan: UVAS Library [Call number: 0195,T] (1).
33.
A Comparative Study Regarding The Immunogenicity Engendered By Three Strains Of Newcastle Disease Vaccine
by Abdus Sattar | Muhammed Akram Muneer | M.Ajmal | T.M.Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1983Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0196,T] (1).
34.
Comparative Studies On The Antibody Titer Of Pigeon Pox Fowl Pox Vaccines In Poultry
by Sajjad Zaheer, malik | Muhammed Yousaf Vaid | Ata-Ur- Rizvi | Muhammed Tufail Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
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Publisher: 1980Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0197,T] (1).
35.
A Study On The Incidence And Types Of Salmonella From Intestines And Liver Of Coturnix Quail Slaughtered At Different
by Fazal Ahmad | Ata-ur- Rehman Rizvi | Mubasher | Muhammed Amin Sheikh | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
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Publisher: 1988Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0200,T] (1).
36.
Comparative Immunogenic Evaluation Of Live Sheep Pox Vaccines Prepared In Vitro And In Vivo
by Anwar Mahmood, M | Ata Ur Rehman Rizvi | Muhammed Yousaf Vaid | Sheikh Altaf | Faculty of Veterinary Sciences.
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Publisher: 1977Dissertation note: Romanian sheep pox virus was pronagate1 in milk teeth susceptible sheep using Borrel techniqne.
The vaccine has a titre of 10-5 in sheep.
A dose of 0.1 ml. of dilution 1:20 was recommended for each sheep. Vaccinated sheep showed a medium size (1 ½ cm- 2 cm) skin reaction at the site of inoculation and mold fever which remained for two days.
Non of the vaccinated sheep showed any secondary lesions or ill effects.
Confluent primary monolayer of testis cells was achieved after 48 hours, CPE started 96 hours post-infection, and 80 percent CF! was noticed on 7th post-infection day.
Vaccine had titre upto 10-4.5 in vitro which correlates The titre in vivo.
0.5 Tissue culture vaccine Containing 100 TCID50 was injected sub/cut to each sheep. It conferred solid immunity.
Both the vaccines withstood challenge infection with a dose of 1 ml (10,000RD) for animal given on 14th post vaccination day.
0.5 ml dose of 1:25 dilution of Romanian virus vaccine gave larger skin reaction which lead to necrosis. Therefore, 0.1 ml dose of 1:20 dilution was used in the study.
5 ml (1.00 TCID 50) of Tissue vaccine when given to sheep did not show any untoward reactions.
Both vaccines gave cross protection against local sheep pox virus and are antigenically related.
Both vaccines showed cross immunity with each other.
The propagation of sheep pox virus in tissue culture is most economical and reliable method.
Tissue culture sheep pox virus vaccine is easy to administer and is recommended for a mass vaccination/sheep pox control programme.
Availability: Items available for loan: UVAS Library [Call number: 0201,T] (1).
37.
Immunogenic Studies On Tissue Culture Rinderpest Vaccine In Buffaloes And Cattle
by Saeed Ahmad | Muhammed Yousaf vaid | Ata ur Rehman Rizvi | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
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Publisher: 1980Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0202,T] (1).
38.
Effect Of Storage Temperature On Pathogenicity On Hydropericardium Syndrome Virus In Chickens
by Arshad Dar, M | Muhammed Akram Muneer | Muhammed | Muhammed Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1991Dissertation note: Present study was designed to work out effect of different storage temperatures, and storage periods on pathogenicity of UPS virus. Observations on demonstration of inmiunosuppresive effect of UPS virus and its antigenic relationship with viruses producing similar type of problems in meat type chickens, were also made. Results of this study have shown no significant difference in virulence upto six weeks of storage at the given temperatures. Disease induction was done in relatively younger birds. Histopathological study of different organs from morbid birds showed changes similar to those reported by other workers.
Immunosuppressive effect of HPSV was demonstrable by low titers of antibodies against NDV and UPS. Indirect haemagglutination test with sensitized human "0" positive erythrocytes for detection of antibodies against HPSV worked effectively. The test is expected to become more sensitive with use of more purified antigen. Sheep RBCs sensitized without use of chromium chloride did not work in IHA test. Slight antIgenic similarity between HPSV and IBD virus iàs demonstrable in Agar gel diffusion test.
Availability: Items available for loan: UVAS Library [Call number: 0210,T] (1).
39.
Immunomodulatory Effects Of Coccidiostats In Broiler Chicks
by Khalid Munir, Ch | Muhammed Ajmal | S.Ata- ur- Rehman Rizvi | tufail | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1991Dissertation note: The birds that received salinomycin at recommended dosage
levels had higher body weight than the monensin treated, cyclophosphamide treated or untreated control birds.
Both salinomycin and monensin treatments did not adversely affect the weights of bursa of Fabricius, spleen, thymus, and livers of birds. Cyclophosphamide treatment of birds in early life resulted in bursal atrophy and slight depression of splenic weight gain.
As compared to cyclophosphamide treated and non-medicated control birds, the sera of NDV vaccinated birds fed salinomycin/monensin had higher antibody titers on day 42. The post-challenge sera of NDV vaccinated birds fed salinomycin, and monensin also had higher antibody titer as compared to NDV vaccinated cyclophosphamide treated birds. The NDV vaccinated birds on salinomycin or monensin medicated rations and those on non-medicated rations did not have any post virulent NDV challenge mortality.
Availability: Items available for loan: UVAS Library [Call number: 0211,T] (1).
40.
Comparative Efficacy Of Haemagglutination Inhibition Test And Enzymne Linked Immunosorbent Assay In Detection Of Antibody Response To Newcastle Disease Virus
by Shafiq Ahmad Noori | Muhammed Akram Muneer | Ehtisham | Muhammed Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1991Dissertation note: The present study was conducted to observe the effects of various temperatures on the immune system, growth rate and various lymphoid organs of chickens.
Five hundred day-old chicks were reared upto six weeks of -age under various temperature ranges. These birds were randomly divided into 5 groups (each consisting of 100 chicks), A, B, C, D and E. Birds in group A were kept at 25°C and given NDV vaccine, birds in group B were kept at 37°C and administered NDV vaccine, birds in group C were housed at 42°C and inoculated with NDV vaccine, birds in group D were kept at ambient temperature and given NDV vaccine and those in group E were kept at 37°C and not vaccinated with NDV.
Blood samples from all the birds were collected on days 1, 7, 14, 21, 28, 35 and 42. Live body weights of the birds were recorded on days 1, 21, 28, 35 and 42. At the termination of the study all the surviving birds were sacrificed to study the various lymphoid organs such as thymus, bursa of Fabricius, spleen and liver.
Heat stress at higher temperature enhanced the immune response of the chicken vaccinated against NDV as compared to the birds kept at lower temperature and non-vaccinated birds. The heat stress at various temperatures did not significantly affect:, the bursal and body weight gain, however, heat stress significantly affected the liver, splenic and thymic weight gain.
Availability: Items available for loan: UVAS Library [Call number: 0216,T] (1).
41.
Role Of Pseudomonas As A Poultry Pathogen
by Khalid Hassan | ata _ Ur- Rehman Rizvi | Muhammed Afzal | S. Shahid | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1991Dissertation note: This research work was undertaken to assess the importance of Pseudomonas aeruginosa as a poultry pathogen. For this purpose 4000 samples comprised of spoiled eggs 1000, dead in shell chicks 1000, chicks dying during first week 1000, subsequent deaths 1000, were prodessed.
Pseudomonas aeruginosa was isolated from 302 birds giving a percentage of 15.1. While examining spoiled hatching eggs Pseudomonas aeruginosa was revealed in 131 eggs giving a percentage of 13.1 and from 134 dead in shell chicks giving a percentage of 13.4.
The agewise incidence of Peudoinonas aeruginosp infection was zero percent in day old chicks, 25.6 percent in one week old, 24.84 percent in 2 weeks old, 12.69 percent in 3 weeks old, 11.76 percent in 8 weeks and 7.46 in 9 weeks, 7.69 percent in 11 weeks old and 9.32 percent in 12 weeks old and no incidence was observed in chicks of less than one week and in chicks beyond 12 weeks of age.
Pathogenicity of the isolate was studied in one day old chicks and 10 days old developing chick embryos. Twenty six out of 30 chicks died within one to 7 days whereas all the embryos died within 24 hours to 48 hours post-inoculation. The post-mortem examination in dead chicks showed haemorrhagic oedema at the site of inoculation and in dead embryos generalized congestion below the skin. Pseudonionas aeruginosa was re-isolated from heart blood and liver of these chicks and from yolk and chorioallantoic fluid of the embryos.
Availability: Items available for loan: UVAS Library [Call number: 0217,T] (1).
42.
Sero-Epidemiology Of Brucelosis In Sheep And Goats At Livestock Production Research Institute (Bahadarnagar Farm)
by Shaukat Yab Khan | MUhammed Akram Muneer | Sheikh Muhammed Amin | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
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Publisher: 1991Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0218,T] (1).
43.
Studies On Clostridial Flora Of Ovine Gut
by Zarar Ahmad | S .Ata -Ur- Rehman Rizvi | Muhammed | Muhammed Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
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Publisher: 1991Dissertation note: Clostridial organisms are a part of the normal flora of animal intestine. Under conditions of stress and intestinal stasis bacteria multiply at a very rapid rate and produce intestinal disorders and other acute septicaemic diseases which may result in high mortality and condemnation of carcass and abortion and loss of milk yield in less severe cases.
Faecal samples of 500 sheep of various ages and both sexes, slaughtered at different abattoirs of Lahore were examined during the present study. Clostridial organisms belonging to different species were isolated from 320(64%) ou,t of 500 animals examined. Based upon morphological, colonial and biochemical characteristics these isolates were identified as Clostridium perfrinens 180(36%), Clostridium apcroenes 113(22.6%), Clostridium tetani 10(2%), Clostridium chauvoei 9(1.8%) and Clostridium botulinum 8(1.6%).
Pathogenicity of the isolates was determined in mice and it was observed that 36.25% of the isolates were pathogenic in this species producing acute septicaemic diseases. Out of the different species isolated 11.11% Clostridium chauvoei, 58.33% Clostridiuin prfrinens and 100% Clostridluin tetani were found pathogenic. Pure cultures of isolated organisms were obtained from the livers, tissues and blood of the inoculated mice.
Clostridia are an important group of pathogenic bacteria of veterinary public health significance, further work is recommended for the identification of the toxigenic groups within each species.
Availability: Items available for loan: UVAS Library [Call number: 0219,T] (1).
44.
Study On Antibody Profile Against Brucella Melitensis (Rev-1 Strain) Vaccine In Sheep And Goat
by Manzoor Ahmad Ghumman | M.Akram Muneer | Mubasher | Muhammed Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1992Dissertation note: A total of 213 lambs/kids (60 lambs and 33 kids at livestock experiment station, Jahangirabad 40 lambs and 26 kids at livestock production research institute, Bahadarnagar and 60 lambs at livestock experiment station Qadarabad) were vaccinated with Brucella melitensis Rev. I vaccine. The lambs/kids, 4-6 months of age, that demonstrated zero antibody titre were selected for the experiment. Each lamb/kids recieved 2 ml of brucella melitensis Rev. I vaccine subcutaneously behind the shoulder. Vacenated lambs/kids were bled on 20,30,60,30,120, 150, 180 and 210 days post-vaccination. Sera were separated to determine the antibody profile using serum agglutination tube test. The antibody titre ranged 1/40 to 1/640, On day 20 post_vaccination which declines sharply. On days 210 post _vacination the antibody titre were from 1/10 to 1/40 in sheep at Livestock experiment station Jahangirabad, Oadirabad and livestock production research institute, Bahadurnagur.
The rate of abortion was 1.36% zero and 0.27% in sheep before vaccination at livestock experiment station, Jahangirabad, Livestock production research institute, Bahadurnagur and Livestock experiment station, Qadirabad respectively. The rate of abortion after vacitnation was nill at all the 3 livestock farms.
The rate of abortion in goat are 24.20% and 0% before vaccination at Livestock experiment station, Jahangirabad and 1ivestock production research institute - Bahadarnagar, respectively. The rate of abortion at livestock experiment station, Jahagirabad and Livestock production research institute, Bahadurnagur was 1.7% and 0% in goats after vaccination, respectively.
Antibody titre of brucella melitensis Rev. I vaccine falls sharply, within two months post-vaccination. A single dose of vaccine provided sufficient field immunization.
Availability: Items available for loan: UVAS Library [Call number: 0228,T] (1).
45.
Serosurveillance Of Brucellosis And Leptospirosis In Horses At Lahore
by Amir Altaf, M | Muhammed Ajmal | M. Akram Muneer | Rashid Ahmed | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
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Publisher: 1992Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0231,T] (1).
46.
Sero-Epidemiological Study Of Brucellosis In Domestic Animals Using Standard Plate Test, Standard Tube Test,
by Chani, M | Muhammed Naeem | Muhammed | Syed Ata- ur - Rehman rizvi | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1992Dissertation note: The sero-epidemlological study of brucellosis was carried out to observe the incidence of brucellosis in 1000 slaughtered end 1000 healthy animals in Peshawar district of N.W.F.P. All the serum samples were subjected to four serological tests I.e. Standard Plate Test, (SPT) standard Tube Test, (STT) Rivanol Test, (RV) and 2,tlercapto-ethanol Test. (2,ME).
The incidence of brucellosis In 1000 healthy animals tested by Standard plate test, standard tube test, rivanol test, end 2,Mercopto-ethanol test was 2.4%, 1.46%, 1.05% end 0.52%, respectively. While the incidence of brucollosis in 1000 slaughtered animals from Peshawar abettior was 2.5%, 1.5%, 1.2% and 0.8% by standard plate test, standard tube test, rivanol test, and 2, Mercopto-ethonol test.
The incidence of the disease was higher in slaughtered animals as compared with healthy animals. The disease was more prevalence In goats as compared to buffaloes, cattle, end sheep.
The Incidence of brucellosis in male animal at slaughter house Peshawar was 5% by standard plate test.
While other three tests were found to be negative. Also the same result was recorded In live male animals.
The Incidence of brucellosis In female animals at slaughter house Peshawar was 2.29%, 1.56%, 1.25% and 0.83% by standard plate test, standard tube test, rivanol test and 2,Mercapto-ethenol test. Similarly it was seen that the disease was more common In aged animals as compares to Youngers stocks.
The efficacy of standard plate test was found more as compared to standard tube test, rivanol test, and 2,Mercepto-ethanol test In slaughtered as well as in apparently healthy animals at Peshawar district. Standard plate test detected 2.45%, where as standard tube test detected 1.80%, rivanol test detected 1.55%, and 2,Mercapto- ethanol test detected 1.05% positive cases in slaughtered as well as In healthy animals.
So the standard plate test was found to be more sensitive, reliable, and can be easily apply.
Availability: Items available for loan: UVAS Library [Call number: 0241,T] (1).
47.
Isolation & Characterization Of Newcastle Disease Virus Strains In Poultry
by Shazia Afzal | Sheikh Muhammed Amin | Asif Rabbani | Muhammed Ajmal | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1992Dissertation note: A study was undertaken to isolate and characterize Newcastle Disease Virus Strains involved in field outbreaks.
A total of 1000 diseased/dead birds ( broilers, layers arid non-discript birds ) were examined for this purpose. The isolation of the virus was attempted from composite samples comprising lungs, liver, spleen, brain and proventriculus of the suspected birds, which was inoculated in embryonated eggs through allontoic cavity route. Out of the total birds examined only 6(0.06%) yielded ND Virus. All the strains present in allontoic fluid gave a positive reaction to spot haemagglutination test, on fowl RBCs. The isolates were confirmed as Newcastle disease pathogen through a positive reaction to haemagglutination inhibition test conducted with known positive sera against Newcastle disease.
Various value values obtained for mean death time (MDT) were 48, 60, 70, 72, 48 and 62 hours for isolates.
No. 1, 2, 3, 4, 5, and £ respectively.. Values of intracerebral pathogencity index far isolated No. 1, 2, 3, 4, 5, and 6 were 1.71, 1.68, 1.58, 1.46, 1.7 and 1.65 respectively. The intravenous pathogenecity index varies recorded were 230, 200, 1.91, 1.88, 2.08 and 1.93 for isolate No. 1, 2, 3, 4, 5, and 6 respectively.
Availability: Items available for loan: UVAS Library [Call number: 0242,T] (1).
48.
Role Of Maternal Antibodies In Protection Against Infectious Bursal Disease (Ibd) In Commercial Broilers
by Zaheer Ahmad | Muhammed Akram Muneer | s. Ata-ur-Rehman Rizvi | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1992Dissertation note: In this study, the decay rate of maternal antibodies to infectious bursal disease virus (IBDV) protective efficacy of maternal antibodies to experimental IBDV challenge and the protective efficacy of a live IBD virus vaccine (Bursine 11) to I8DV experimental challenge were studied. Enzyme Linked Immunosorbent 1ssay (ELISP) was standardized and performed to analyze the serum samples.
The level of maternal antibodies to (IBDV) was found to be positive till the fourth week of age. The line of best fit to this decay rate within the study period was found to be Y=0.43-O.O53 (week) and has the coefficient of determi- nation 0.904. The positive level of maternal antibodies after second week of age were found insufficient to protect broiler chicks against the IBDV challenge. Maximum mortality (207.) was observed after experimental IBDV challenge at 35 days of age in broiler chicks. This suggested that the offsprings need to be vaccinated with the live IBD virus vaccine before that age.
The maternal antibodies were demonstrated to interfere with the live vaccination.
vaccination. Live virus at 14 days of age were found to be protective and a booster with the same vaccine in the fifth week of age prevented the chicks from infection.
Availability: Items available for loan: UVAS Library [Call number: 0250,T] (1).
49.
Studies On Anaerobic Ruminal Bacterial In Nili-Ravi Buffaloes
by Sajjad Hussain, S | S.Ata-Ur-Rehman Rizvi | Muhammed Akram Muneer | Rashid Ahmed | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Nature of contents: ; Literary form: Publisher: 1992Dissertation note: 1000 samples of ruminal fluid, 500 from each of the two experimental Nili-Ravi buffaloes, kept in Nutrition Section of College of Veterinary Science Lahore were examined for ruminal flora. One quarter of sample 1rom each animal were used for the isolation of proteolytic, amylolytic and lypolytic bacteria.
From these samples 617 species of bacteria were Isolated and identified on the basis of morphological, cultural and biochemical characteristic. Number of species of different bacteria isolated were Streotococcus bovis 229 (109 from tiuffalne A and 120 from buffaloe B.), Selenomonas ruminantium 16 (9frorn buffaloe A and 7 from buffaloe B), Bacterolde amylophilus 83 (28 from buffaloe A and 55 from buffaloe B); Bacteroides ruminicola 79 (36 from buffaloe A and 43 from buffaloe B), Anaerovibrio lvoolvtica 47 (19 from buffaloe A and 28 from buffaloe B), Bacteroides succiriopenes 59 (31 from buffaloe A and 28 from. buffaloe B), Butyrivibrio fibrisolvens 40 (25 front buffaloe from A and 15 from buffaloe B), and Ruminncoccus fjvifacieris 64 (26 from buffaloe A and 38 from buffaloe B).
It was observed that both the experimental buffaloes, maintained on the same diet, had a similar type of bacterial flora, the differences being in number only.
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50.
Studies On Isolation, Identification And Pathogenicity Of Bacteria Isolated From The Respiratory Tract Of Cattle And Buffaloes At Lahore Abattoir
by Yousaf, M | Muhammed akram Muneer | Ata-Ur- Rizvi | Imtiaz Hussain Khan | Faculty of Veterinary Sciences.
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Publisher: 1992Dissertation note: total of 1200 samples consisting of 300 nasal, 300 tracheal, 300 bronchial swabs and 300 pieces of lung tissue from 150 randomly selected cows and buffaloes (75 from each species) were collected from abattoir of Lahore Corporation. Of the total samples 702 isolates were isolated and identified on the basis of their morphological, cultural, and biochemical characteristics. The isolates included Pdsteurellp multocida 89 strains, (45 strains from buffaloes and 44 strains from cows), Pasteurella haemolvtica 98 strains (59 from buffaloes and 39 strains from cows), Corynebacterium ovocenes 76 strains (54 strains from buffaloes and 22 strains from cows), Escherichia coli 146 strains (80 strains from buffaloes and 66 strains from cow), Staphylococcus epidermidis 152 strains (75 strains from buffaloes and 77 strains from cows), Bacillus subtilit 135 strains (67 strains from buffaloes and 68 strains from cows), and Mycobacterium bovis 6 strains (2 strains from buffaloes and j strains from cattle).
The isolation percentage of Pasteurella. multocida was 19.66% in buff aloes and 13% in cattle, 15% Pasteurella haemolytica was present in buffaloes and 14.66% Pasteurella haemolvtica in cows. 18% and 7.33% Corvnebacterium pyogenes could be isolated from buffaloes arid cattle respectively. 26.66% and 22% Escherictiia coli was isolated from buffaloes and cattle respectively. From buffaloes and cows 25 and 25.66% of Staohvlococcus epidermidis was isolated respectively. Bacillus subtilis was 22.33% and 22.66% in buffaloes and cows respectively 2.66% and 5.33% Mvcobacterium bovis was isolated from buffaloes and cattle respectively.
There was small difference in the number of isolates from buffaloes and cattle. Pasteurella haemolytica, Staphyloicoccus epidermidis and Bacillus subtilis proved to be nonpathogenic while Pagsteurella multocida, Corynebacterium pyogenes ai.d Escherichia coli caused l00, 50 and 59 percent mortality on experimental inoculation. Mycobacterum bovis was non pathogenic for mice. However upon intraperitoneal inoculation in rabbit it caused 57% mortality.
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